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1 OVINE AND CAPRINE BRUCELLOSIS PROGRAMS IN CYPRUS- 2013 SCOFCAH June 2014
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OVINE AND CAPRINE BRUCELLOSIS PROGRAMS IN CYPRUS- 2013

SCOFCAH June 2014

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Infected herds: Elimination program

Non infected herds: Declaration and maintenance of the Brucellosis officially free status

APPLIED PROGRAMMES

• Vaccination prohibited since 1978

• Treatment of brucellosis in farm animals is prohibited by law

• Notifiable disease since 1978

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• A Committee considers depopulations, declaration of holdings as infected or non infected and assessment of laboratory results in cooperation with DVOs.

• The Animal Health and Welfare Division is designing the sampling programme based on the Brucellosis and the animal registration and identification databases for each District. The programs are followed up and revised at least three times a year.

• The Animal Health and Welfare Division has created a system of documents and instructions for uniform implementation of the program in all districts

• One Veterinary Officer in each District is responsible for the application of the programs, under the supervision of the District Veterinary Officers.

• Veterinary Inspectors and Veterinary workers apply the program

Director

Serology and Bacteriology Laboratory

Veterinary Laboratories

Animal Health and Welfare Division

Animal Health and Welfare Division

Veterinary Public Health Division

District Veterinary Offices

BRUCELLOSIS INFORMATION AND WORK FLOW CHART

COMMITTEE

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SYSTEM OF DOCUMENTS

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SHEEP AND GOAT FLOCKS: EXAMINATION SCHEMES

DECLATION OF OFFICIALLY FREE STATUS:Negative Rose Bengal Test at all animals over 6 months old twice at an interval of more than six months and up to 12 months

All the animals have been free from clinical signs of brucellosis

MAINTENANCE OF OFFICIALLY FREE STATUS:Rose Bengal Test in representative number of animals according to Annex 4, Chapter 1, Par B of Directive (EC) 91/68.

POSITIVE FLOCKS:CFT and culture

PREMOVEMENT TESTIf animals originate from an officially free herd then only the animals moving are examined with Rose Bengal test

If animals originate from a non officially free herd then all animals in the herd are examined with Rose Bengal test

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NON-NEGATIVE RESULTS PROTOCOL IN SHEEP AND GOATS

INITIALLY LOW RISK FLOCKS: Flocks with one RB +, CFT-animal.

INITIALLY MEDIUM RISK FLOCKS: Flocks with more than one animal RB+, CFT – or RB+, CFT to the limits of the laboratory uncertainty (20-33.3 ICFTU/ml)

INITIALLY HIGH RISK FLOCKS (POSITIVE FLOCKS) : Flocks with one or more animals RB+, CFT+ (>33.3 ICFTU/ml)

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INITIALLY LOW-MEDIUM RISK FLOCKSIn initially low and medium risk flocks:

Movement restrictionsCulling of animal/animalsRepeat blood test of the culled animal/animals Cultures from the culled animal/animals Epidemiological investigation

Interpretation of all information from the Committee and FINAL ALLOCATION to: false positive A (final allocation only for initially low risk flocks) : If epidemiological information is negative for brucellosis, culture is negative and the serology the same or negative) then movement restrictions are withdrawn. False positive B (final allocation for initially low and medium risk flocks): If epidemiological information is negative for brucellosis, culture is negative, and blood tests the most RB+ and CFT+ (<33.3ICFTU/ml), then whole flock examinations follow, with confiscation of RB+ and CFT+ (<33.3ICFTU/ml) animals, until the whole flock is negative. Then measures are withdrawn. Positive (final allocation for all flocks): All flocks that show (initially or at the animal/flock re examination of the initially low and medium risk flocks) at least one animal RB+, CFT + (>33.3ICFTU/ml) or show a positive culture. Withdrawal of restrictive measures after four consecutive negative tests in a period of six months

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POSITIVE FLOCKS- measuresRestriction of all farm movementsEpidemiological investigationCulling of seropositive animals and compensation of the ownersCollection of milk from negative animals in the infected farms in separate milk tanks and reassurance of pasteurizationCleaning and disinfection of infected farms, burial of the pasture or collection and disinfection in a place far from establishments Serological examination of dogs or other species of animals susceptible to Brucellosis found in infected farmsSerological reexamination (Rose Bengal and CFT in parallel) of all bovine over twelve months old or sheep and goats over six months old every month after the slaughter of positive animalsWithdrawal of restrictive measures after four consecutive negative tests in a period of six monthsIn case of depopulation restocking at least after 6 months

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Sheep and Goat Flocks 2013

Total sheep and goat herds, under the program : 3.035% coverage of herds tested: 97,5%

No. of positive herds: 0No. of new positive herds: 0

Total animals under the program : 505.500% coverage of animals tested: 41,20%

No. of positive animals:0Total number of animals slaughtered: 30 (false positives)

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GRAPH 1: BRUCELLOSIS IN SHEEP AND GOATS IN CYPRUS

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GRAPH 2: OFFICIALLY FREE SHEEP AND GOAT FLOCKS AND ANIMALS

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Brucellosis Officially Free Member Staterequest

On 30/4/2013, after 13 years of program implementation, Cyprus requested from the European Commission the Brucellosis (Brucella melitensis) Officially Free Member State StatusCurrently we are working on the additional data requested from the Commission

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BOVINE BRUCELLOSIS PROGRAMS IN CYPRUS- 2013

SCOFCAH June 2014

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BOVINE HERDS: EXAMINATION SCHEMESDECLATION OF OFFICIALLY FREE STATUS:

Farms with over 10 animals: three negative tests in bulk milk samples (at least 30% of cows in lactation) at three-monthly intervals followed at least six weeks later by negative Rose Bengal test at all animals over 12 months old

Farms with less than 10 animals: two negative Rose Bengal tests at an interval of more than three months and up to 12 months at all animals over 12 months old

All the animals have been free from clinical signs of brucellosis

MAINTENANCE OF OFFICIALLY FREE STATUS:Three negative tests in bulk milk samples (at least 30% of cows in lactation) at three-monthly intervals or two negative Rose Bengal tests at all animals over 12 months old annually

POSITIVE HERDSIn case of a positive bulk milk test, Rose Bengal test, CFT test

Culture in serologically positive animals

PREMOVEMENT TESTIf animals originate from an officially free herd then only the animals moving are examined with Rose Bengal test

If animals originate from a non officially free herd then all animals in the herd are examined with Rose Bengal test

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Total bovine herds, under the program : 306 % coverage of herds tested: 99,02%

No. of positive herds: 0No. of new positive herds: 0

Total animals under the program : 39.689coverage of animals tested: 99,92%

No. of positive animals: 0Total number of animals slaughtered: 0

Bovine Herds 2013

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Prevalence and Incidence of Brucellosis in Cattle in Cyprus(last outbreak in 2008)

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