of 23
8/18/2019 PAA Efficacy Time Temp
1/23
EFFICACY OF
PEROXYACETIC ACID
TIME VS. CONCENTRATION
VS. TEMPERATURE AGAINSTBACTERIA, YEASTS, MOLDS, AND SPORES
8/18/2019 PAA Efficacy Time Temp
2/23
These charts include 6 bacteria species, 5 yeasts, 4 molds, and 6 types of
spores. The 100% Kill Times represent the time to completely eradicate
(100%) of each indicated species. This chart is unusual in that it does notreport the time needed to reduce the given organism to particular log
reductions, which typically would be 5-6 logs.
All challenge organisms started with levels of the challenge from 107 to
108 populations.
These challenge tests would be appropriate for peroxyacetic acid
formulas containing approx. 5.5% PAA and 26-27% hydrogen peroxide.
Other formulas may not perform to the standards and results reported
herein.
Intro
8/18/2019 PAA Efficacy Time Temp
3/23
100% Kill Time of Staphylococcus aureus(gram-positive bacteria)
0.00.5
1.0
1.5
2.0
2.53.0
3.5
4.0
4.5
5.0
5.5
110 275
Active PAA (ppm)
E l a p s e d T i m
e ( m i n s . ) 41 F
50 F
68 F
104 F
Staphylococcus aureus-GP bacteria
8/18/2019 PAA Efficacy Time Temp
4/23
Streptococcus faecalis-GP bacteria
100% Kill Time of Streptococcus faecalis(gram-positive bacteria)
0.0
0.5
1.0
1.5
2.0
2.5
3.0
110 275
Active PAA (ppm)
E l a p s e d T i m e ( m i n s . )
41 F
50 F
68 F
104 F
8/18/2019 PAA Efficacy Time Temp
5/23
Aerobacter aerogenes-GN bacteria
100% Kill Time of Aerobacter aerogenes(gram-negative bacteria)
0.0
0.5
1.0
1.5
110 275
Active PAA (ppm)
E l a p s e d T i m e (
m i n s . )
41 F
50 F
68 F
104 F
8/18/2019 PAA Efficacy Time Temp
6/23
Pseudomonas aeruginosa-GN bacteria
100% Kill Time of Pseudomonas aeruginosa(gram-negative bacteria)
0.0
0.5
1.0
1.5
2.0
2.5
3.0
110 275
Active PAA (ppm)
E l a p s e d T i m e
( m i n s . )
41 F
50 F
68 F
104 F
8/18/2019 PAA Efficacy Time Temp
7/23
Salmonella typhimurium-GN bacteria
100% Kill Time of Salmonella typhimurium(gram-negative bacteria)
0.0
0.5
1.0
1.5
2.0
2.5
3.0
110 275
Active PAA (ppm)
E l a p s e d T i m e ( m i n s . )
41 F
50 F
68 F
104 F
8/18/2019 PAA Efficacy Time Temp
8/23
Salmonella dublin-GN bacteria
100% Kill Time of Salmonella dublin(gram-negative bacteria)
0.0
0.5
1.0
1.5
2.0
2.5
3.0
110 275
Active PAA (ppm)
E l a p s e d T i m e
( m i n s . )
41 F
50 F
68 F
104 F
8/18/2019 PAA Efficacy Time Temp
9/23
Sacchromyces cerevisiae-yeast
100% Kill Time of Sacchromyces cerevisiae(yeast)
0.0
5.0
10.0
15.0
20.0
25.0
110 275
Active PAA (ppm)
E l a p s e d T i m e
( m i n s . ) 41 F
50 F
68 F
104 F
8/18/2019 PAA Efficacy Time Temp
10/23
Sacchromyces diastaticus-yeast
100% Kill Time of Sacchromyces diastaticus(yeast)
0
1
2
3
4
5
6
110 275
Active PAA (ppm)
E l a p s e d T i m
e ( m i n s . )
41 F
50 F
68 F
104 F
8/18/2019 PAA Efficacy Time Temp
11/23
Candida mycoderma-yeast
100% Kill Time of Candida mycoderma(yeast)
0
20
40
60
80
100
120
140
110 275
Active PAA (ppm)
E l a p s e d T i m e
( m i n s . )
41 F
50 F
68 F
104 F
8/18/2019 PAA Efficacy Time Temp
12/23
Hansenula anomala-yeast
100% Kill Time of Hansenula anomala(yeast)
0
20
40
60
80
100
120
140
110 275
Active PAA (ppm)
E l a p s e d T i m e (
m i n s . )
41 F
50 F
68 F
104 F
8/18/2019 PAA Efficacy Time Temp
13/23
Pichia membranaefaciens-yeast
100% Kill Time of Pichia membranaefaciens(yeast)
0
20
40
60
80
100
120
140
110 275
Active PAA (ppm)
E l a p s e d T i m e
( m i n s . )
41 F
50 F
68 F
104 F
8/18/2019 PAA Efficacy Time Temp
14/23
Penicillium camerunense-mold
100% Kill Time of Penicillium camerunense(mold)
0
20
40
60
80
100
120
140
110 275
Active PAA (ppm)
E l a p s e d T i m
e ( m i n s . )
41 F
50 F
68 F
104 F
8/18/2019 PAA Efficacy Time Temp
15/23
Aspergillus niger-mold
100% Kill Time of Aspergillus niger (mold)
0
50
100
150
200
250
300
110 275
Active PAA (ppm)
E l a p s e d T i m e
( m i n s . )
41 F
50 F
68 F
104 F
8/18/2019 PAA Efficacy Time Temp
16/23
Mucor spececium-mold
100% Kill Time of Mucor spececium(mold)
0
50
100
150
200
250
300
110 275
Active PAA (ppm)
E l a p s e d T i m e
( m i n s . )
41 F
50 F
68 F
104 F
8/18/2019 PAA Efficacy Time Temp
17/23
Geotrichum candidum-mold
100% Kill Time of Geotrichum candidum(mold)
0
10
20
30
40
50
60
70
110 275
Active PAA (ppm)
E l a p s e d T i m e
( m i n s . )
41 F
50 F
68 F
104 F
8/18/2019 PAA Efficacy Time Temp
18/23
Bacillus cereus-spores
100% Kill Time of Bacillus cereus(spores)
0
10
20
30
40
50
60
70
275 412.5 550
Active PAA (ppm)
E l a p s e d T i m e
( m i n s . )
41 F
50 F
68 F
104 F
8/18/2019 PAA Efficacy Time Temp
19/23
Bacillus subtilis-spores
100% Kill Time of Bacillus subtilis(spores)
0
10
20
30
40
50
60
70
275 412.5 550
Active PAA (ppm)
E l a p s e d T i m e
( m i n s . )
41 F
50 F
68 F
104 F
8/18/2019 PAA Efficacy Time Temp
20/23
Bacillus mesentericus-spores
100% Kill Time of Bacillus mesentericus(spores)
0
10
20
30
40
50
60
70
275 412.5 550
Active PAA (ppm)
E l a p s e d T i m e ( m i n s . )
41 F
50 F
68 F
104 F
8/18/2019 PAA Efficacy Time Temp
21/23
Thermophilic species-spores
100% Kill Time of Thermophilic spore-formers
0
10
20
30
40
50
60
70
275 412.5 550
Active PAA (ppm)
E l a p s e d T i m e
( m i n s . )
41 F
50 F
68 F
104 F
8/18/2019 PAA Efficacy Time Temp
22/23
Clostridium perfringens-spores
100% Kill Time of Clostridium perfringens(spores)
0
10
20
30
40
50
60
70
275 412.5 550
Active PAA (ppm)
E l a p s e d T i m e ( m i n s . )
41 F
50 F
68 F
104 F
8/18/2019 PAA Efficacy Time Temp
23/23
Clostridium specialis-spores
100% Kill Time of Clostridium spec.(spores)
05
10
15
20
25
30
35
40
45
275 412.5 550
Active PAA (ppm)
E l a p s e d T i m e ( m i n s . )
41 F
50 F
68 F
104 F
