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Paleopalynology
By: Group 5Analisa
Ramnarine Ronny
Rahman
PangaeaSupercontinent
Triassic Period. Mixed mesophytic forest
China to the middle section of the North American continent.
Mixed Mesophytic
Forest•Grown in/ adapted to a moist environment. •Fossil bed in Idaho is a result of the forest, and has left behind remains of plant and animal species
PurposeTo separate pollen, spores
and remains of once living organisms
No Hydrofluoric Acid
To match the pollen and spores to their original
Materials. Clay . Hydrogen
peroxide. Computer .
Hyposodium Chlorite
. Distilled water
. Meshed screen (300)
. Electric scale
. Microscope
. Filter . Mortar and pestle
. Glacial Acetic Acid
. Pipettes
. Glycerin . Slides/ cover slips
. Glycerin . Sodium Hydroxide
. Hydrochlori
c Acid
. Test tubes
Protocol And Analysis #1 Mortar and pestle 300 mesh
screen1 part hydrochloric Acid to .2g clay4 part glacial acetic acid to .2g clayAdd a drop of the diluted acid to slide
+ clay.Observe under microscope
Protocol And Analysis #1 Mortar and pestle 300 mesh
screen1 part hydrochloric Acid to .2g clay4 part glacial acetic acid to .2g clayAdd a drop of the diluted acid to slide
+ clay.Observe under microscopeNot very usefulGlacial acetic acid attacks anything
with calcium in it
Protocol And Analysis # 2
3% hydrogen peroxide added to the 300 mesh screened clay
pipette to add a few drops of the diluted acid to the slide
Protocol And Analysis # 2
3% hydrogen peroxide added to the 300 mesh screened clay
pipette to add a few drops of the diluted acid to the slide
hydrogen peroxide did not change much
Protocol # 3Trial 1 Trial 2
Add 35 drops of glacial acetic acid
Add 35 drops of Sodiumhypo Chlorite
Set time limits of 5 min/30 min/45 min/ 1 hour/ 1 day
Set time limits of 5 min/30 min/45 min/ 1 hour/ 1 day
Dilute with tap water Dilute with tap water
Filter, then let it air dry over night.
Filter, then let it air dry over night.
Add a small amount of the treated clay to two clean slides.
Add a small amount of the treated clay to two clean slides.
Use a pipette to add 1 drop of distilled water to one slides, and 2 drop of glycerin to the other.
Use a pipette to add 1 drop of distilled water to one slides, and 2 drop of glycerin to the other.
Analysis # 3Same broken pieces of barrels and pollen in slides Chemicals did not destroy pollen, but the mortar and pestle
Protocol And Analysis #4
Clay from the block + a drop or two, of distilled water unto the slide.
Place a cover slip on it and observe under microscope.
Protocol And Analysis #4
Clay from the block + a drop or two, of distilled water unto the slide.
Place a cover slip on it and observe under microscope.
Simple and easy to do so many preserved parts of
organisms and pollen that we have captured