ParasitologyHeamofragellaeJMJ

O Great St. Joseph of Cupertino who while on earth did obtain from God the grace to be asked at your examination only the questions you knew, obtain for me a like favors in the examinations for which I am now preparing. In return I promise to make you known and cause you to be invoked.

Through Christ our Lord.St. Joseph of Cupertino, Pray for us.

Amen.

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LEICHMANIA

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Life Cycle

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Leichmania amastagote stage• Found within phagocytic vacuoles of

• macrophages and other mononuclear phagocytes.

• Size : 2-5um x 1-3um.

• Shape : Oval or rounded.

• Other features : Parasitic nuclei and kinetoplasts are arranged to give the “Dot and Dash” appearance.

• Demarcation of each amastigote is not apparent.

• No external flagellum.

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Leichmania amastagote stage

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Leichmania promastagote stage• Found both in midgut and pharynx of sandfly vector and in

culture media.

• Size : 15-20um in length, 1.5-4.0um in width.

• Shape : varies from pyriform to long slender spindle shape.

• Kinetoplast : Placed anterior to the nucleus.

• Flagellum : Arises at the anterior end.

• No undulating membrane.

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Leichmania promastagote stage

Anterior Kinoplast

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Leichmania promastagote stage

Anterior Kinoplast

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Leichmania mucocutaneous lesion

Anterior Kinoplast

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Leichmania cutaneous lesion

Anterior Kinoplast

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TRYPANASOMABRUCI

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Trypansoma bruci life cycle

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Trypamastagote stage

• Size : 15-35um x 1.5-4.0um.

• Shape : Fusiform protozoa, flattened from side to side.

• Pleomorphic with long and slender to short and stumpy forms.

• Tapering anterior end and blunt posterior end.

• Nucleus : Pleomorphic, varies with regard to size and position.

• Kinetoplast : Not prominent.

• Flagellum : Free flagellum, can vary in size.

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Trypanasoma bruci

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TRYPANASOMACRUCI

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Trypansoma cruizi life cycle

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Trypamastagote stage

• Size : 17-30um in length.

• Shape : Fusiform slender protozoa, flattened from side to side.

• Typically ‘C’ shaped.

• Tapering anterior end and short

• pointed posterior end.

• Nucleus : Deeply staining, centrally placed, oval shaped nucleus.

• Kinetoplast : Large and prominent. Situated at the posterior end (Terminal).

• Flagellum : Projects from the anterior end, after passing along the undulating membrane.

• Free flagellum about 1/3 rd of the body length.

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Trypanasoma cruizi

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HemofragellaeLeichmania Trypanasoma bruci Trypanasoma cruzie

AmastagotePromastagote

Epimastagote Trypamastagote

AmastagoteEpimastagoteTrypamastagote

Sand fly Tsetse fly Tryatominae bug

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Hemofragellae

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