P I O N E E R I N G D I A G N O S T I C S
Pathogen Detection and Quality Indicators
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Raw MaterialEnvironmental SamplesSemi-finished Products
Finished ProductsMicrobial detection
LEADER IN INDUSTRIAL MICROBIOLOGYFOOD INDUSTRY APPLICATIONS
Media Preparation
Sample Preparation
Enumeration Identification
The food microbiology pathway: from sample to decision
Tempo®
Masterclave®
APS ONE®
Dilumat® Smasher® Labguard®
VIDAS®
VITEK® MS
VITEK® 2 Compact
API
IT Solutions
ALOA Plate
D-Count®
Gene-Up*
* Available soon
Pathogen Detection
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GENE-UP®
GENE-UP®
Real-Time PCR Pathogen Detection System• PCR-based molecular diagnostic system for the detection of
bacteria and viruses in food, raw materials and the production environment
• 6-Channel Multiplex qPCR Thermocycler with Routine Software and Open Software
• Combination of BioFire’s know-how in molecular biology and bioMérieux’s expertise in microbiology
• Added value: detection of food pathogen bacteria and viruses, precision of PCR molecular biology, improved lab productivity and limited risk of inter-sample contamination
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GENE-UP REAGENTS
• Generic 5 min. Mechanical Lysis• 192 Ready-to-Use lysis tubes
per kit• Room Temperature Storage• Patented Magic Cap• Red Lysis ensures traceability
• Salmonella, Cronobacter, Listeria spp, Listeria mono, E. coli O157:H7, STEC
• 192 PCR tests per kit• Room Temperature Storage• Internal Control in every test• Blue dye ensures traceability
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Amplification & Detection
SPIN DOWN(10 sec)
ENRICHMENT in X mL BPW Broth
Selective AGAR
ConfirmationSample Prep
Transfer 10µl Lysate intoPCR TUBES
PCR Tubes
Transfer20µL into
LYSIS TUBES(Red Dye)
MECHANICAL LYSIS
(15 min) PCR
GENE-UP METHOD: SIMPLIFIED WORKFLOW WITH THE GENEPROOF ASSAY
Transfer 30µl of
GENEPROOF PCR MIX
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FRET TECHNOLOGY PRINCIPLE
Target
640 nm detection
470 nm light
Internal Amplification
Control
705 nm detection
470 nm light
640 nm detection
705 nm detection
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GENE-UP PROBE-BASED MELT PEAK ANALYSIS
Melt peak analysis is more sensitive than other real time PCR and is
valuable for detecting low level samples.
IncreaseTemperature
Cool PCR reaction
Probes bind DNA
Probes melt off DNA
High Fluorescence
Low Fluorescence
negative derivative
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GENE-UP PROBE-BASED MELT PEAK ANALYSIS
Sample 13 amplified poorly during PCR but was called positive by melt and confirmed by culture
Low level sample + negatives
Amplification Curves
Melting peaks
This is Confidence
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GENE-UP ADVANTAGES
3 Levels of Specificity with FRET Technology Internal Control with every test Rapid, Standardized Workflow for all assay parameters
- <30 minutes sample prep time for 96 tests Ultra Fast and Efficient mechanical lysis procedure Only two enrichment broths used:
- BPW for GENE-UP E. coli O157:H7, STEC, Salmonella spp. and Cronobacter
- LPT Broth for GENE-UP Listeria spp. and L. monocytogenes
Assay Mix & Match Capability Open Platform
- foodborne virus detection, GMO, allergen testing, etc. Remote Access Software Room Temperature reagent storage bioMérieux Service and Support
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GENE-UP: WORLD WIDE VALIDATIONS
Full Suite of AOAC-RI and ISO/AFNOR Validations
Salmonella
E. coli O157:H7
Listeria spp.
L. monocytogenes
EHEC Series
Cronobacter*
Various Matrices and Sample Sizes
*Cronobacter assay offers enrichment harmony with Salmonella assay
P I O N E E R I N G D I A G N O S T I C S