PCR AccessoriesA range of accessories to help impove sample handling, PCR results and to simplify the PCR work-flow
NEW
Ergo Stand For sample set-up we offer the Ergo Stand whose fully adjustable position helps to reduce repetitive strain disorders from pipetting.
Ergo Freeze Blocks The Ergo Freeze blocks can be used in combination with the Ergo Stand or on their own to keep samples at 4oC during PCR set up.
White Marker Pen Our white marker pen allows effective labelling of coloured plates to reduce sample errors.
Adhesive Sealing For improved sealing performance of adhesive seals we offer a number of solutions like our optical film compression pads, FrameStar® plate adapter and adhesive seal roller.
Plate Cutter We are very proud to present the latest addition to our range: the 4titude® Plate Cutter. Depending on the blades used it can cut plates or seals in strips or partial plate sections.
www.4ti.co.uk | 49 +44 (0)1306 884885
The Ergo Stand is designed for holding microplates and PCR plates during pipetting. Skirted plates sit directly onto the stand, non-skirted, semi-skirted , strips of tubes and single tubes can sit within a plate adapter (4ti-0960/X-F), supplied free with the Ergo Stand.
The height and angle of the Ergo Stand can be adjusted for maximum pipetting comfort, to reduce stress and strain on the upper body and to help prevent repetitive strain disorders (RSI) and work-related upper-body limb disorders (WRULD).
The stand includes eight spaces for 1.5ml or 2.0ml microcentrifuge tubes. This is especially useful when pipetting master mixes into PCR plates.
For colour coding or identification of your work, we offer the Ergo Stands in four colours: red, yellow, green and blue.
Ergo Freeze Personal Cooling Blocks
Use of the Ergo stand can be combined with the Ergo Freeze cooling blocks (for details see page 51)
Ergo Stand
Code Description Quantity
4ti-0393/B Ergo Plate Stand, Blue 1 stand
4ti-0393/R Ergo Plate Stand, Red 1 stand
4ti-0393/G Ergo Plate Stand, Green 1 stand
4ti-0393/Y Ergo Plate Stand, Yellow 1 stand
4ti-0960/X-F Ergo Plate Adapter (for use with non-skirted
and semi-skirted PCR plates) 1 adapter
| www.4ti.co.uk50 +44 (0)1306 884885
PC
R A
ccessories
White Marker Pen
Ergo Freeze
Pierce Plate
This marker pen is ideal for labelling our Vision Plates™, FrameStar® Plates and FrameStrips™. The resulting white labelling is clear to see on all colours of plate. Once dry this water resistant ink cannot be removed using aqueous or organic solvents.
These SBS-sized cooling blocks, available in pink and yellow, are the perfect PCR accessory. Once frozen for 8 hours at -20°C, they turn from pink to purple or yellow to green and maintain 4°C for approximately 2.5 hours. At 7°C the block returns to its original colour, clearly indicating when it is no longer refrigerating the samples.
The Pierce plate is a metal tool with 96 sharp metal spikes aligned central to each well of a 96well plate. The Pierce plate can be easily positioned over a sealed plate and with minimal pressure, will pierce all 96wells simultaneously. Each well may then be easily accessed with a single, 8, 12 or 96 tip manual or automated pipette.
The Pierce plate can puncture most heat and adhesive seals.
• Idealforlabellingcolouredmicroplates
• Impervioustoaqueousandorganicsolvents
• Lowodour
• SBSformat
• Maintainssamplesat4°Cforupto2.5hours
• SuitableforallPCRplatesandtubes
• CompatiblewithErgoStand
• Colourchangeclearlyindicatesdurationofcooling
• Piercesacomplete96wellplateinonemotion
• Easytouse
• Savetime
Code Description Quantity
4ti-0394 White marker pen 1 pen
Code Description Quantity
4ti-0398 Pierce Plate 1
Code Description Quantity
4ti-0395 Ergo Freeze Personal
Cooling Block, pink 1 Ergo Freeze
4ti-0396 Ergo Freeze Personal
Cooling Block, yellow 1 Ergo Freeze
www.4ti.co.uk | 51 +44 (0)1306 884885
PC
R A
cces
sori
es
Optical Film Compression Pad
The Optical Film Compression Pad is a soft silicon foam laminated to a non-stick film. When used in conjunction with adhesive seals, such as the qPCR adhesive seal (4ti-0560), the pad enhances the adhesion between the seal and the PCR plate. In turn, this improves results by reducing the chance of sample evaporation.
• Improvedadhesivesealperformance
• 96windowsenablinguseonqPCRinstruments
• Compatiblewithheatedlidcyclers
• ReducesPCRfails
• Reducedwelltowellvariation
FrameStar® 384 Plate Adapter
Adhesive Seal Roller
The plate adaptor offers on effective platform for efficient sealing of 384well PCR plates using adhesive seals. Its design ensures that the plate is held perfectly flat so that even pressure can be applied with the Adhesive Seal Roller (4ti-0502).
Our Adhesive Seal Roller is of a durable plastic construction with a padded roller. It is recommended for use with all our adhesive seals.
• Ensuresevenandconsistentapplicationofadhesivesealsto384wellplates
• Supportsall384wellsevenly
• Forbestresults,usewithourAdhesiveSealRoller
• Completeandsecureapplicationofadhesiveseals
• Consistentsealapplicationacrossallwells
• Recommendedforusewithall4titude®adhesiveseals
Code Description Quantity
4ti-0391 FrameStar® Plate
Adapter for adhesive seals 1 Adapter
Also see
Adhesive seAling (pAges 76-83)
Also see
Adhesive seAling (pAges 76-83)
Code Description Quantity
4ti-0502 Roller for adhesive seals 1 roller
Code Description Quantity
4ti-0563 Optical film compression pad 5 pads
| www.4ti.co.uk52 +44 (0)1306 884885
PC
R A
ccessories
Plate Cutter
The semi-automatic Plate Cutter (4ti-3000) can be used with various blade options to create either strips or partial plates from sealed or unsealed Break-A-Way plates. Alternatively, when placed on a different support plate the instrument can be used to cut an adhesive or heat seal on a plate without cutting the plate itself.
The FrameStar® Break-A-Way plate is positioned on the plate carrier and the drawer pushed fully into the instrument. After locking the draw, the cutting action is activated and the air pressure powered cutting blades roll over the plate cutting it into the segments or scoring the plate seal.
Available as 8well strip cutter, a 12well strip version will be launched in 2014.
• CutssealedorunsealedBreak-A-Wayplates
• Idealforkitmanufacturing
• Workswithwiderangeofadhesiveandheatseals
Technical specifications
• Dimensions:210x460x450mm
• Weight:30kg
• Airpressure5.5-6.0bar(80-87psi)
Also see
break-a-way (pages 26-29)
NEW
Code Description Quantity
4ti-3000 PlateCutter, including air 1
tubing and user manual
4ti-3010 Single Strip Cutter Head 1
4ti-3020 Double Strip Cutter Head 1
4ti-3030 Triple Strip Cutter Head 1
4ti-3040 4-Strip Cutter Head 1
4ti-3050 Half-Plate Cutter Head 1
www.4ti.co.uk | 53 +44 (0)1306 884885
BarcodingAll skirted and semi-skirted plates are available with linear barcodes for identification and traceability. The labels are highly scratch resistant and can withstand cold storage (-80°C) and solvents, such as DMSO. Either single or double barcodes are available off-the-shelf and a variety of customisations are possible.
Standard Barcoded PlatesOur standard barcoded plates are available off-the-shelf, single or doubled barcoded for all our fully-skirted and semi-skirted plates. The barcodes are printed in Code 128 which is compatible with most barcode readers. In addition, human readable format is printed to the right of the label, ensuring samples can always be identified.
The single barcode is positioned on the south side of the plate and the double barcode is additionally positioned on the east side of the plate.
To order either version, add /SBC or /DBC to the ordering code of the respective plate.
Custom Barcoded PlatesShould you require other barcoded options, not offered from our standard barcoding option, our custom service can apply them in your preferred configuration or format, with a starting sequence of your specification. We can apply a variety of barcode formats to any side of the plate.
Custom barcode orders should be submitted together with a completed custom barcode order form. This can be downloaded from www.4ti.co.uk/technical/barcode.php
Position of Standard Barcode Labels
Label Specification
Do
ub
le B
arc
od
e
Single Barcode
Dimension Min. Max.
A Label length - 46 mm
B Code length - 25 mm
C Quiet zone 3 mm -
D Label width 5 mm 6 mm
E Code width 5 mm 6 mm
A
B C
4T105246
C
D E
| www.4ti.co.uk54 +44 (0)1306 884885 | www.4ti.co.uk54 +44 (0)1306 884885
Barcode ReaderWe offer two different barcode scanner.
Single code 2D readerThe smaller 2D barcode Reader (4ti-0787) is compact and scans single 2D barcodes from FrameStrips, Cryotubes or tubes from other manufacturers. Barcodes are instantly read and displayed. The included software is user-friendly and allows for reliable and convenient sample management.
2D BarcodingIn addition to our standard and custom linear barcoding we also offer 2D barcoding. 2D barcoding is the use of a datamatrix with a defined number of fields to encode alphanumerical information. The code uses data redundancy so even when codes are partly destroyed the information will be retained.
For our Break-A-WayTM plates and FrameStrips we offer off-the shelf 2D barcodes.
For ordering please refer to the product pages 28 to 29 and 40 to 41.
Code Description
4ti-0787 Single code 2D barcode reader including software
4ti-4000 Single code 2D barcode rack scanner
Rack scannerOur new rack scanner (4ti-4000) scans 2D barcoded tubes in an SBS footprint or cryorack format. Different masks are available for the different consumables. After the scanning the data can be exported in a number of file formats for use with sample management systems
Additional servicesWe offer a number of additional services including:
• Sterilization by gamma irradiation
• Double ethylene oxide treatment
• Custom packaging eg, single wrapping
• Products in non-standard colours
Please contact us to inquire about additional charges and minimum order quantities for your specific custom requirements.
NEW
www.4ti.co.uk | 55 +44 (0)1306 884885 www.4ti.co.uk | 55 +44 (0)1306 884885
4titude’s® 4LAB™ is an automated, high precision, pipetting system specifically designed for low-volume liquid handling. Low volume pipetting requires accuracy and consistency, which when performed manually, is tedious, time consuming and prone to human error.
The 4LAB™ guarantees accuracy, precision and consistency, and reagent wastage is reduced. Unlike complex, multi-purpose robotic systems, our instrument was designed for the researcher without prior robotic experience. Intuitive set-up and programming will save your laboratory time and money instantly.
The 4LAB™ is available as a 4+2-position or 6+2 position workstation and with different optional modules like HEPA filter and UV lamp or active heating and cooling (coming soon).
LAB
| www.4ti.co.uk56 +44 (0)1306 884885
Liquid Handling
Figure 1. Excellent Serial Dilution Results7μl NIH/3T3 Cell cDNA sample is diluted with 21μl water at 1:4 ratio, 4 times. Roche® LightCycler® 480 realtime PCR thermal cycler and Finnzyme DyNAmo® Flash SYBR® Green qPCR kit (F-415L) were used.
Figure 2. Superior Precision Results vs Manual PipettingTarget: Human GAPDH (top curve) was amplified in 4 replicates. 18μl master-mix and 2μl cDNA were pipetting into 20μl reaction volume. Roche® LightCy-cler® 480 real-time PCR thermal cycler and Invitrogen™ Platinum® Taq DNA Polymerase were used.
Figure 3. Cost SavingA 4LAB™ customer cut reagent costs by 40% and labour costs by 50% by adopting the 4LAB™ in their SNP assay project.
4LAB™: Ct std dev = 0.135
Manual Pipetting: Ct std dev = 0.145
Simple and Intuitive Software• The 4LAB™ control software can be mastered in an hour.
• No off-site training or dedicated technician is required.
• Protocols can be quickly created and modified and transferred via USB memory stick.
• Extensive open labware database is pre-installed
• Software and labware database updates are avaialable at no additional cost
• Sample concentration normalization programs for tube and plate formats are included as standard
Versatile and Adaptable• 10 interchangeable standard microplate / tip rack adapters
and 5 interchangeable reagent vessel adapters available for use with tubes (0,5 - 5ml) tube strips and plates (6, 12, 24, 48, 96 and 384well) or different reservoirs
• 5 cooling blocks can keep sensitive reagents and samples cold
• 4 automatic pipetting modules are available
• Optional modules with UV lamp and HEPA filters or active heating and cooling capabilities
Affordable Solution“40% reagent costs and 50% labour costs were saved by adopting the 4LAB™ and 384well PCR microplates in our SNP assay project… ...the 4LAB™ provided the high accuracy and precision we needed.“
Mr Sheng-Jan Lin, Director of the Contract research Department, Seeing Bioscience Co. (Figure 3).
HEPA filter removes 99.97% of particles of a size of 0.3 micrometres or larger
100%
40%
Reagent Cost Labour Cost
50%Cost saving
90%
80%
70%
60%
50%
40%
30%
20%
10%
0%
Highly Accurate and Precise• All automated pipetting modules are
calibrated to ISO-8655 standards.
• Accurate pipetting results for the complete volume range of 1ul to 200ul.
• Excellent results for qPCR standard curve and replicates (Figure 1).
• Better Precision of pipetting results than manual pipetting (Figure 2).
UV light to eliminate DNA contamination
NEW
NEW
www.4ti.co.uk | 57 +44 (0)1306 884885
Liqu
id H
andl
ing
Notebook Computer with Software
• Pre-installed software
• Windows® 7 operating system
• 15.6” monitor
Reagent Area
• 2 interchangeable tube or reservoir holders
• Peltier-controlled active cooling blocks are available for various reagent vessels
4+2 position or 6+2 position Worktable
• 1 or 2 tip racks and up to 5 plate or tube holders can be placed in the interchangable positions
Disposable Used Tip Tray
• Capacity > 300tips
Functional Safety Housing
• Safety door detection for emergency stop
• Clear acrylic material, UV impermeable, tinted material with UV / HEPA module
Compact Design
• Small footprint, sits neatly on a standard lab bench
Easy to Service• The automated pipetting modules are simple to remove
and can be returned to 4titude® for service and calibration.
• Only minimal maintenance of the 4LAB™ is necessary
• The entire system is compact and light-weight.
Automated Pipetting Modules (APM)• Single or 8-channel with 1-50ul and 10-200ul
• 4LAB™ automatically recognises the APM installed
• Easy to change
• HEPA filtration with UV lamp option
COMING SOON
• Active heating / cooling block adaptersNote: According to ISO-8655 standards (Gravimetric method), APM is calibrated in temperature (21-25°C, ±0.5°C) and humidity (60-90%) controlled environment. Twice-distilled water, robotic tips and microbalance were used.
Performance
50µl Tip on single or 8-channel pipetting module 1µl 50µl
200µl Tip on single or 8-channel pipetting module 10µl 200µl
Accuracy (Rel.) ±7% ±1%
Precision (Rel. CV) ≤ 7.5% ≤ 0.4%
Accuracy (Rel.) ±3% ±0.8%
Precision (Rel. CV) v≤ 1% ≤ 0.15%
NEW
| www.4ti.co.uk58 +44 (0)1306 884885
Liquid HandlingUsed Tip Tray
4ti-2061Elevated 384-well PCR Adapter 4ti-2053
1.5ml (20) tube adapter 4ti-2060
Elevated 96-well PCR Adapter 4ti-2052
Deep-well plate adapter h4ti-2063
1.5ml (4x2) tube adapter 4ti-2054
2ml (3x2) & 5ml (1) tube adapter 4ti-2055
8-Channel Pipetting Reservoir 4ti-2062
Illumina 48well adapter 3 x 8 tube strip adapter
Trademarks: Beckman®, Biomek® 3000 (Beckman Coulter Inc.); Roche®,
LightCycler® 480 (Roche Group); DyNAmo™ (Finnzyme Oy); SYBR® (Molecular Probes
Inc.); Invitrogen™ Platinum® (Invitrogen Corp.)
Note: Specifications are subjected to change without notice.
Function: Liquid (Sample/Reagent) Transfer(LT)
Multiple Dispense (MD)
Serial Dilution (SD)
Hold (Pause)
Mixing (MIX)
Loop
Automated Pipetting: Interchangeable single/8-channel,
Module: Volume ranges of 1 - 50μl,
10 - 200μl
Connection: RS-232, USB2.0
Power Supply: 100~240V, 50/60 Hz, 100W
Size (W x D x H): 590 x 440 x 460 mm
Weight (N.W.): 25 kg
For optional modules:
UV Lamp wavelength 254nm (UV-C)
Irradiation capacity of >40uW/cm2
HEPA system with 2x 3M Air-MateTM HEPA Filter
and >200 l/min volume flow
Technical Specification
Ordering Information
Code Description Quantity
4ti-2000 4LAB™ 4-Position System
with Notebook Computer and Control Software 1
4ti-2200 4LAB6 6-Position System
with Notebook Computer and Control Software 1
4ti-2010 50μl Single Channel Pipetting Module 1
4ti-2020 50μl 8-Channel Pipetting Module 1
4ti-2030 200μl Single Channel Pipetting Module 1
4ti-2040 200μl 8-Channel Pipetting Module 1
4ti-2051 96 Tip Rack Adapter 1
4ti-2052 Elevated 96-Well PCR Plate Adapter 1
4ti-2053 Elevated 384-Well PCR Plate Adapter 1
4ti-2054 1.5ml Tube Adapter, 4 x 2 1
4ti-2055 2ml (3x2) & 5ml (1) Tube Adapter 1
4ti-2056 Cooling Block for 96-Well PCR Plates 1
4ti-2057 Cooling Block for 384-Well PCR Plates 1
4ti-2058 Cooling Block for 1.5ml Tubes (4x2) 1
4ti-2059 Cooling Block for 2ml (3x2) and 5ml (1) Tubes 1
4ti-2060 20x1.5ml Tube Adapter 1
4ti-2061 Used Tip Tray, Disposable 10
4ti-2062 8-Channel Pipetting Reservoir 1
4ti-2063 Deep-Well Plate Adapter 1
4ti-2064 Reservoir (80ml), disposable 20
4ti-2100 50μl Pipette Tip, Non-Sterile 10x96
4ti-2101 200μl Pipette Tip, Non-Sterile 10x96
4ti-2104 50μl Pipette Tip, Filtered, Sterile 10x96
4ti-2105 200μl Pipette Tip, Filtered, Sterile 10x96
4ti-2065 Cooling Block for 20x1.5ml Tubes 1
4ti-2066 HLA Typing 96-well Terasaki Tray Adapter 1
4ti-2067 HLA Typing 60 and 72-well Terasaki Tray Adapter 1
4ti-2068 0.5ml Tube Adapter For 4ti-2060 1
4ti-0270 96well conical bottom PCR adapter 1
4ti-0271 Cell culture plate adapter 1
4ti-2072 Illumina 48well adapter 1
4ti-2073 3 x 8 tube strip adapter 1
www.4ti.co.uk | 59 +44 (0)1306 884885