AUTOCRIT II Centrifuge

AND

ADAMS Micro-Hematocrit I I Centrifuge

OPERATORS MANUAL

Clay Dtv~sion 01 Adams Beclon. D~ckinson and Company lBFjl

AUTOCRIT II

IMPORTANT INSTRUCTIONS 220V LlNE CORD

This Centrifuge is equipped with a CEE-Approved Type 220V Line Cord and Plug, which is wired as indicated.

LlNE CORD PLUG/WIRE COLORS

(Hot) Black I I Brown (Live)

(Common) White I Light Blue (Neutral)

(Ground) Green f Green/Yellow (Earth) I

Replacement Parts are available as follows:

REPLACEMENT CENTRIFUGE I PAFIT I

1 0106-001-000 1 DYNAC II Centrifuge ( 01 06-608-000 1 0206-001 -000

Clay Adams * Dlvlslon ot

Breton Dlrltincnn and company

TRIAC Centrifuge

0592-300-000

0594-1 00-000

0562-001-000

0568-001-000

raaoratory tnstrumentation

0 206-606-000

0568-000-002 REV. A (1/82)

Insert in Manual for Fulura Reference on Replacement Parts

READACRIT Centrifuge

M P READACRIT Centrifuge

MHCT II Centrifuge

AUTOCRIT II Centrifuge

Centrifuge Model 0574 Model 0568

(120 volt Base Model 0558) (220 volt Base Model 0562)

0592-61 8-000

0592-61 8-000

0562-620-000

0562-620-000

I

AND

. Micro-Hematocrit II Centrifuge Models 0556, 0557 Models 0565,0566

(120 volt Base Model 0558) (220 volt Bare Model 0562)

OPERATORS MANUAL

CAUTION I READ THIS MANUAL THOROUGHLY BEFORE OPERATING

THE INSTRUMENT I

ADAMS, AUTOCRIT, MICROTAtNER, PRE-CAL, REDI-LANCE, SEAL-EASE, VACUT' YANKEE, 00 , & and B-0 are trademarks of Becton, Dlckinson and Cornpant) - -

T

Copy right @ 1976 by CLAY ADAMS, Division of Becton, Dickinson and C p p I

CONTENTS

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . I . INTRODUCTION I . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A . INTENDED USE 1

. . . . . . . . . . . 6 . SUMMARY AND EXPLANA TION OF TEST 2 . . . . . . . . . . . . . . . . . . . . C . PRINCIPLES OF PROCEDURE 2

. . . . . . . . . . . . . . . . . . . . . . . . . . It . AUTOCRITIICentrifuge 3 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A.USE 3

. . . . . . . . . . . . . . . . . . . 8 . INSTALLA TION PROCEDURES 4 1 . Scale Plate, Carrying Tray and

. . . . . . . . . . . . . . . . . . . . . . . . . Magnifierlnstallation 4 2.SpareParts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

. . . . . . . . . . . . . . . . . . . . . . . . . 3 interchangeable Heads 5 . . . . . . . . . . . . . . . . . . . . . . . . . 4 . Power Requirements 5

. . . . . . . . . . . . . . . . . . . . C . PRINCIPLESOFOPERATION 6 D . PERFORMANCE CHA RACTERlSTlCS AND

. . . . . . . . . . . . . . . . . . . . . . . . . . . . SPECIFICA TIONS 6 . . . . . . . . . . . . . . . . . . . . E . OPERA TlNG INSTRUCTIONS 7

. . . . . . . . . . . . . . . . . . . . . . . 7 . Using the Carrying Tray 7 . . . . . . . . . . . . . 2 OperatingtheAUTOCRlTliCentrifuge 8

. . . . . . . . . . . . . . . . . . . . . . . . . . 3 Reeding Hematocrits 8 . . . . . . . . . . . . . . . . . . . F . CALIBRATION PROCEDURES 9

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . I . Speed Check 9 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 Timercheck fO . . . . . . . . . . . . . . . . . . . . . G OPERATING PRECAUTIONS 10 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . I Basic Precautions 10 . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 OperatorTreinhg 11

H.HAZARDS. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . I 1 . . . . . . . . . . . . . . . . . . . . I MAINTENANCE AND SERVICE I f

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 . Maintenance I I . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 . Setvice 13

. . . . . . . . . . . . . . . . I / l . A DAMS Micro-Hematocrit I1 Centrifuge 14 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4 . USE. 14

. . . . . . . . . . . . . . . . . . . 8 . INSTAL LA TION PROCEDURES 15 . . . . . . . . . . . . . . . . . f . Head and Head Cover Installation 15

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 . S p m P m 16 3 . Conversion to Direct-Reading AUTOCRIT I I

Centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 . . . . . . . . . . . . . . . . . . . . . . . . . 4 . power Requirements 16 . . . . . . . . . . . . . . . . . . . . C . PRINCIPLES OF OPERATION 17 D . PERFORMANCE CHA RACTERISTICSAND . . . . . . . . . . . . . . . . . . . . . . . . . . . . SPECIFICATIONS 17 . . . . . . . . . . . . . . . . . . . . E . OPERATING INSTRUCTIOIVS 77 . . . . . . . . . . . . . . . . . . . . . . . . 7 . Filling Capillary Tubes 17 2. Operating the A DAMS Micro-Hematocrit 11 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Centrifuge. 57

. . . . . . . . . . . . . . . . . . . . F . CALIBRA JION PROCEDURES 18 . . . . . . . . . . . . . . . . . . . . . G OPERATING PRECAUTIONS 18

H HAZARDS . . . . . . . . . . . . . . . . . . . . . . . 18 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 . MAINTENANCEANDSERVICE 18

I V . SPECIMEN COLLECTION. PREPARATION. AND . . . . . . . . . . . . . . . . . . . . . . . STABILITY 1 . . . . . . . . . . . 18

. . . . . . . . . . . . . . . . . . . . A . FINGER PUNCTURE BLOOD 18 B . VENOUS BLOOD . . . . . . . . . . . . . . . . . ) . . . . . . . . . . . . 20 C . INTERFERING SUBSTANCES . . . . . . . . . . . . . . . . . . . . 20

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . I . An ticoagulants 20 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 . Drugs 21 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . D STORAGE 21

. . . . . . . V . TEST PROCEDURES FOR MICRO-HEMATOCRITS 21

. . . . . . . A . MATERIALS REQUIRED. BUT NOTPROVIDED 21 . . . . . . . . . . . . . . . B . USING THEAUTOCRITII Centrifuge 21

C . USING THE ADAMS Micro-Hematocrit 11 Centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

VI . TEST RESULTSAND LIMITATIOYS . . . . . . . . . . . . . . . . . 22

. . . . . * . . . . . . . . . . . . . . . . . . . . . VII.' EXPECTED VALUES : 22

. . . . . . . . . V / I l SPECIFIC PERFORMANCE CHARACTERISTICS.. 22

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . IX . BIBLIOGRAPHY 22

. . . . . . . . . APPENDIX A Disassembly and Replacement Procedures 23 . . . . . . . . . . . 1 Inspection and Replacement of Motor Brushes 23

. . . . . . . . . . . . . . . . . . . . . . . 2 Motor and Spindle Assembly 24 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 . Timer 26

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 ONLamp 27 . . . . . . . . . . . . . . . . 5 . Latch. Switch and Solenoid Assembly 27

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 Lidsafety Switch 28 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 Varktor 28

. . . . . . . . . . . . . . . . . . . . . 8 . Linecord and Plate Assembly. 28 . . . . . . . . . . . . . . . . . . . . 9. Lid and Hinge Block Assemblies 29

APPENDIX 8 . List of Spare Parts. Head Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . and Accessories 30

iii

I . INTRODUCTION .

This Manual contains operating instructions for the follo\;ring centrifuges:

Description A UTOCR IT I I Centrifuge with Head

Power R'ating Modet No, 1 20 vdl ts 0574

AUTOCRIT I I Centrifuge with Head 220 volts ADAMS Micro-Hematocrit I I Centrifuge

with 24-Place Head t 20 v ~ l t s ADAMS Micro-Hematocrit I I Centrifuge

with 24-Place Heed i

220 volts ADAMS Micro-Hemetocrit t I Centrifuge 1

with 16-Place Combination Head 120 vajtts ADAMS Micro-Hematocrit I I Centrifuge i

with 16-Place Combination Head 220 vhlts

Centrifuge Base Only without Head 120 v+ts 0558 Centrifuge Base Only without Head 220 volts I 0562

Operating instructions for the AUTOCRiT I 1 Centrifuge are described in Section I I and, for the ADAMS Micro-Hematocrit I I ~ehtrifuge, in Section 1 1 I .

I Where instructions are the same for both centrifuges, thk operator is referred to , the appropriate paragraph in Section I I. I

I I A, INTENDED USE

AUTOCRIT I1 and ADAMS Micro-Hematocrit II Centrifuges (Figure 1) are designed to obtain quantitative micro-hematocrits (MHCT) of whole blood specimens.

Figure 1. A UTOCRl T I1 Centrifuge (/eft) and A DAMS ~icro-dematocrit I1 Centrifuge. I

The AUTOCRIT I I Centrifuge has a built-in spiral scale plate and magnifier to permit the reading of hematacrit values directly in the centrifuge head.

The ADAMS Micro-Hematocrit ll Centrifuge requires a separate measuring device to read the hematocrits of centrifuged specimens.

0. SUMMARY AND EXPLANATION OF TEST

'Micro methods for the determination of hematocrits have been used widely in clinical laboratories for many years. The results of micro-hernatocrit deter- minations are recognized as being fully equivalent to the results of macro deter- minations. The more commonly used micro methods require approximately 65 cu.mm (.065 mlJ of blood and only about 5 minutes or less of centrifugation, compared to the macro method of Wintrobe(l), which requires at least 1 ml of blood and up to 30 minutes of centrifugation.

C, PRINCIPLES OF PROCEDURE

The normal micro-hematocrit technique utilizes a small glass capillary tube, coated internally with an anticoagulant (heparin). Plain capillary tubes can be used if the blood sample has been previously anticoagulated. The capillary tube i s filled with blood obtained directly from a finger puncture or a VACUTAINER Brand Blood Collection Tube or syringe. The dry end of the capillary tube is sealed with sealing clay (Figure 2). The tube i s then placed in a speciat micro- hematocrit centrifuge and rotated at high speed for 3-5 minutes. After centri- i~lgat ion, the blood sample is separated into two fractions (Figure 3) : a column of piicketi red cells and a column of clear plasma.

Sealing Clay --------/

Figure 2. 8lood.Filled Capillary Tube Be fore Centrifugation.

H Y p l a r r n s *-- Packed Red Cells --+I Figure 3. Capillary Tube A fter Centrifugation.

The hematocrit (HCT) is the ratio of the packed red cell volume to the volume of the original blood sample, multiplied by 100. Since the capillary tube has a uniform bore, the hematocrit may be determined by measuring the two lengths as shown in Figure 3. The hematocrit value may then be re-defined as follows:

Usually the two lengths are measured with the aid of a mechanical measuring device. Such a device presents the final hematocrit value; no computations are required by the operator.

The AUTOCRIT I! Centrifuge has a built-in hematocrit scale plate and trans- parent tube-carry ing tray, designed to accommodate 75 mm capillary tubes. Hematocrits of centrifuged blood specimens are read directly in the instrument by measuring the length of the packaged red cell column against the built-in hematocrit scale.

The ADAMS Micro-Hematocrit I! Centrifuge requires the use of a separate measuring device to read the hernatocrit.

1 I. AUTOCRlT II Centrifuge (Model Nos. 0574 and 0568)

A. USE

The AUTOCRIT I1 Centrifuge is a high speed instrumen! that permits the quantitative measurement of micro-hematocrits direct1 y in tbe centrifuge head.

Main components and features of the AUTOCRIT II centrifuge are shown in Figure 4. The head assembly consists of a spiral hematocrit scale plate and a rotating carrying tray that holds up to twenty-four 75 mm capillary tubes. After centrifugation of tubes, hematocrits are read directly against the scale plate, eliminating tho need for a separate measuring device,

The carrying tray consists of a clear disc mounted within a o~btted outer rim and center hub, The center hub contains rubber flaps that hoId,the capillary tubes in place, During centrifugation, leakage of sealing material and blood from the tubes i s prevented by a rubber liner in the outer rim of the tray. The protective

lRYlNO TRAY

2,

length of column of packed red cells = overall length of blood sample

x 100 Figure 4. A U JOCRI T I / Centrifuge and Head Components

head cover f i ts over and locks the carrying tray to the motor spindle. A plastic magnifier supplied with the centrifuge simplifies the accurate reading of hematocrits.

B. INSTALLATION PROCEDURES

After unpackaging, place the centrifuge on a steady, level working surface. The scale plate, carrying tray, head cover and magnifier are packaged separately and must be installed as described below. NOTE: A spanner wrench is secured by a chain to one of the rubber feet and is used to tighten the scale plate locknut and to remove the head cover.

1. Scale Plate, Carrying Tray and Magnifier Installation

a. Open the centrifuge lid by pulling the latch handle forward. Unscrew the locknut from the threaded spindle. NOTE: The spindle is permanently secured to the motor shaft and cannot be removed.

h. Place the scale plate over the spindle and rotate the plate until the two pins projecting from the spindle s/ip into the two holes in the bottom of the scale plate.

c. Thread the locknut onto the spindle and hand-tighten. Hold the scale plate as shown in Figure 5 and tighten the locknut securely, but not excessively, with the spanner wrench.

d. Place the carrying tray on the scale plate by placing the center hub over the spindle.

e. Before centrifuging capillary tubes, the head cover must be placed over the carrying tray and tightened on the spindle by turning the knurled head cover knob until it engages the index pin projecting from the hub of the carrying tray. The knob is then tightened by hand. Do not tighten the head cover knob with the spanner wrench. NOTE: When the head cover is not in use, place it inside the safety liner of the lid, This will prevent the operator from closing the lid without first instatting the head cover.

f. Install the magnifier (see Figure 4) by inserting the magnifier post through the hole in the top right deck of the centrifuge housing, NOTE: The magnifier can be scratched if handled incorrectly. When removed from the centrifuge, do not lay the magnifier on its polished viewing surface. To remove smudges and fingerprints, wash the magnifier in mild detergent and water and wipe dry with a soft tissue or cloth.

2. Spare Parts

Spare rubber liners for the outer rim and center hub of the carrying tray and one spare set of motor brushes are shipped with the centrifuge. The brushes are packaged in a cloth bag secured inside the bottom cover of,the machine, Brush and liner replacement procedures are described under Subsection 1 I- I - MAINTENANCE AND SERVICE.

3. Interchangeable Heads

The head assemblies listed below may be used interchangeably in the Centrifuge in place of the standard scale plate, carrying tray, and head bover. Neither head assembly offers the direct reading feature of the standard AUTOCR IT !I Centri- fuge head assembly.

1

16-Place Combination Head with Cover (Catalog No. 0564): a multi-purpose head that holds eight 75 mm or 60 mm capillary tubes, plus eight 75 mm x 8 mm serum tubes or eight MICROTAINER Capillary Blood Serum Separators.

24-Place Head with Cover (Catalog No. 0563): accommodates twenty-four 75 rnm or 60 mm capillary tubes. I I

NOTE: The above head assemblies are supplied as standard equipment with models of the ADAMS Micro-Hematocrit 1 1 Centrifuges described in Section I I I of this Manual.

4. Power Requirements

Connect the power cord only to a 3-wire grounded power receptacle rated as follows:

Model 0574 AUTOCR IT l l Centrifuge: 120 volts, 60 t-lz, 15 amps

Mode! 0568 AUTOCR IT l l Centrifuge: 220 volts, 50 Hz, 7.5 amps

Figure 5. Securing Scale Plate and Locknut with Spanner Wrench.

4

1

CAUTION

Connect only t o an approved power source such as a 3-wire grounded receptacle. Where a 2-wire receptacle i s encountered, have it replaced with a properly grounded 3-wire receptacle in accordance with the National Electrical Code. Do not, under any circumstances, remove the grounding prong from the power cord plug.

Should the power cord'become cracked, frayed, broken or other- wise damaged, have it replaced immediately by qualified service personnel.

Unplug the power cord before servicing. Parts replacements should be made only by trained service personnel and according to the instructions in DISASSEMBLY AND REPLACEMENT PROCE- DURES, Appendix A, of this Manual. For assistance with service problems, call Clay Adams Customer Service Department: (201) 887-4800.

C. PRINCIPLES OF OPERATION

The Autocrit I1 and Adams Micro-Hematocrit I I Centrifuges are single speed machines. Their controls and operating features are identical, as described below.

The basic centrifuge consists of a motor and spindle assembly mounted by v~l~ri l t ion isolators within a high strength molc!ed housing. The housing is attached to i, t~ottorn cover assembly which rests on four suction-type ru1)bc.r feet.

Posit ive safety systems and interlocks, intended to fulfill the national consensus sa fe ty standards, are built into the centrifuge to give the operator maximum protection.

The lid assembly, which is made of high-strength steel, also contains a steel safety liner. When the lid is closed, the safety liner completely shields the operator from the centrifuge head compartment.

The centrifuge is started by depressing the PRESS TO START Button in the center of the TIMER. The centrifuge cannot be started until the lid is c/osed and latched. Once the centrifuge is started, a latch interlock system prevents the lid from being opened until &e centrifugation is complete. A red ON lamp on the front of the centrifuge remains on during operation and turns off when the lid lock is disengaged.

0. PERFORMANCF CHARACTER ISTlCS AND SPECIFICATIONS (All Models of AUTOCR IT I I and ADAMS Micro-Hematocrit I I Centrifuges)

I? Speed (@ rated voltage) : 1 1,700 rpm, +22%, -10% 0 Relative Centrifugal Force (nominal) : 13,700 RCF U Timer: Electric, graduated in one minute increments up to 15 minutes.

Recommended MHCT Centrifugation Time: Three minutes for complete cell packing,

D Lid Safety Switch - actuated by a pin on the centrifuge lid. The centrifuge is inoperable unless the safety switch i s actuated.

El Latch Lock - automatically actuated by latch solenoid when lid is closed and centrifuge is started. Automatically unlocked on completion of centrifugation. Power Requirements (Motor) : Models 0574,0556 and 0557: I20 VAC, 60 Hz, 1.8 amps Models 0568,0565 and 0566: 220 VAC, 50 Hz, 0.6 amps

0 Motor Brake - electric type, automatically actuated by electric timer after completion of selected spinning time.

O Power Cord - UL Listed 2.1 m (7 ft), strain-relieved power cord with 3-prong plug.

0 Weights {Max.) : Centrifuge with Head : 10.19 kg (22.5 Ib) Shipping Weight: 12.91 kg (28.5 Ib) Dimensions: Closed Machine: 22.22 cm (8.75 in) high x 25.70 cm (10.12 in) wide x

31.90 crn (12.56 in) deep Shipping Container: 38.10 cm (15 in) high x 38.10 cm (15 in) wide x

45.72 cm ( 18 in) deep

E. OPERATING INSTRUCTlONS

1. Using the Carrying Tray

The carrying tray can be token on hospital rounds by the! lab technologist and loaded as each filled capitlary tube is properly sealed, [see Section IV - SPECIMEN COLLECI1ON, PREPA.RATION, AND STABILITY). For maximum efficiency in collecting specimens, the use of several trays is recommended. Trays can be individually identified by marking the frosted spot on the center hub (see Figure 6) with a pencil. Capillary tubes can be transported, stored, centrifuged and read without removing them from the tray,\

To load the carrying tray, insert the unsealed end of the cbpillary tube into one of the hub slots, as shown in figure 6, and drop the sealed :end into the opposite rim slot. Continue to load the capillary tubes in a balanced array.

FROSTED SPOT

Figure 6. Inserting Capittary Tubes into Carrying Tray.

The carrying tray accommodates 75 mm capillary tubes, including the following:

0 PRE-CAL Heparinized Capillary Tubes" (Catalog No. 1025) - Heavy wall, narrow lumen tubes, requiring about 1/3 the blood needed by standard tubes and of particular value in pediatric work.

0 Plasmacrit Heparinized Capillary Tubes (Catalog No. 5435) - For use in rapid screening tests for ~ ~ ~ h i l i s . ( * ) ~ ( ~ )

2. Operating the AUTOCRIT If Centrifuge

a. Place the loaded carrying tray over the scale plate.

b. Spin the head by hand to check for proper seating and position of the tubes.

c . Place the head cover over the carrying tray so that the spindle fits into the threaded hole in the center of the head cover. Make sure that the index pin on the carrying tray hub (see Figure 4) fits into one of the index holes on the underside of the head cover, A good method to insure proper indexing while tightening the head cover is to hold the cover knob firmly with one hand and spin the entire head assembly counterclockwise with the other hand.

d. Tighten the head cover securely by hand. Do not tighten with the spanner wrench.

e. Close the centrifuge lid and press down until the latch handle is fully retracted. The centrifuge will not operate unless the lid is closed.

f . T t ~ r n the TIMER dial clockwise to the desired setting.

!I. Press the PUSH TO START Button in the center of tlie TjMER, The centrifuge head will immediately accelerate and the ON lamp will go on and remain on until the centrifugation i s complete and the lid lock i s disengaged. NOTE: Once the TIMER is set, i t does not require resetting before additional centrifugations calling for the same spinning time.

h. After the ON lamp goes off and the head slows, pull the latch handle forward to open the lid. Remove the head cover by holding the knurled knob with one hand and rotating the head assembly clockwise with the other hand. Use the spanner wrench if the cover is too tight to be loosened by hand.

i. Read hematocrits of the capillary tubes following the procedures in Paragraph 3, below.

j . For additional centrifugations at the same time setting, repeat Steps a-e, above, and start the centrifuge by depressing the PUSH TO START Button.

3. Reading ~ematoc;its

Use the magnifier supplied with the AUTOCRIT t l Centrifuge for accurate hematocrit readings. To read hematocrits:

a. Swing the magnifier over the spiral hematocrit scale.

I,. Rotate the carrying tray until the tube to be read i s at the front of the centrifuge, directly under the center of the magnifier.

' P R E-CAL Capillary Tubes contain 0.5 (minimuml U.S. P. units of ammonium heparin pur rube.

c. Slide the capillary tube along i ts axis until the interface between the bottom of the packed red cell column and the sealing material coincides with the "0" line at the periphery of the spiral scale (see Figure 7).

d. Hold the aluminum rim of the carrying tray steady with one hand. Use the other hand to rotate the scale plate until the top of the plasma column coincides with the " 100%" I ine on the spiral scale (see Figure 7).

Figure 7, Direct Hernetocrit Reeding from Scdle Plate.

e. The hematocrit value may now be read from the spiral scale at the inter- face between the packed red cell column and the clear plasma column.

f. After all the tubes have been read, remove them from the tray by care- fully sliding each tube out o f its hub slot and then lifting it off the tray. Discard the capillary tubes safely in accordance with approved laboratory procedures.

F. CALIBRATION PROCEDURES

1. Speed Check

The AUTOCR IT I I and ADAMS Micro-Hematocrit I I Centrifuges are fixed-speed machines designed to rotate at 11,700 rpm fully loaded. Speed must be checked with the head assembly installed and the lid closed. Speed checks should be made with an accurate tachometer, such as an ADAMS Photo Electric Tachom- eter, Model 5205. Mechanical tachometers that contact the motor spindle should be avoided. The lid has a hole in the center to. provide access for a tachometer.

I f the speed of th-e centrifuge i s outside specified limits (i,e., 11,700 rpm, +22%, IWX,), chock the supply voltage with an accurate monitor, Ruled spcud i s

obtained by operation of the centrifuge at the line voltage and frequency recorded on the data plate of the instrument. Deviations in line voltage or frequency will affect the operating speed of the centrifuge. I f line voltage and frequency are correct, but rated speed i s not obtained, there is an internal rnajfunction in the centrifuge. Contact your nearest Clay Adams dealer for service, o r call Clay Adams Customer Service Department: (201) 887-4800, for assistance.

2. Timer Check

Timers in the AUTOCRlT II and ADAMS Micro-Hematocrit II Centrifuges should be checked occasionally for accuracy with a stopwatch as follows:

a. Close and latch the lid. b. Turn the timer dial to three minutes.

c. Press the PUSH TO START Button and simultaneously actuate the stopwatch,

d. Check the iength of time for the PUSH TO START Button to release with the stopwatch. I f the timer and stopwatch do not agree within f 10 seconds, turn the timer dial hack to zero, then reset the dial to three minutes, Repeat the check several times, each time turning the dial back to zero and resetting it to three minutes.

e . I f a timer setting cannot be found which agreeswithin f 10 seconds of the stopwatch, the timer should be replaced. (See Appendix A, DISASSEMBLY AND REPLACEMENT PROCEDURES.)

G. OPERATING PRECAUTIONS 7 . Basic Precautions

I n order to obtain properly centrifuged specimens and to prevent damage to the centrifuge, the following basic operating precautions should be carefully observed :

[I Elec t r i ca l : Operate the centrifuge only a t the tine voltage and frequency specified on the data plate,

Load Balancing: For smooth operation and long service life, it is important that capillary tubes are instal led in a balanced array.

tnstalting Head Cover: Always install the head cover before operating the centrifuge.

0 Timing: For accurate results, follow the recommended timing routines specified in this Mtnual.

O M HCT Values: Accurate hematocrit determinations depend upon proper blood collection and handling techniques. Carefully observe the collection procedures described in this Manual.

0 Cleanliness: Keep the centrifuge clean and dust-free in accordance with Maintenance and Service Instructions supptied in this Manual. Avoid spilling liquids into the centrifuge.

Inspect the motor brushes for wear a t six-month intervals. Brushes should be r.cplaccd when they are worn to approximately 5 mm or 3/76",

2. Operator Training

Thc ccntritt~gc i s ;In c1cctric:nl inslru~nont ticsignad to produco quantit;~[ivc! micro-hematocrit determinations for medical purposes, Use of this instrument for patient medicat evaluation places a responsibility upon administrative personnel for the adequate training of operators in i ts safe and effective use.

Administrative personnel should make certain that operators and technologists receive adequate instruction and training before they are permitted to operate the centrifuge.

H. HAZARDS

Basic safety precautions must be observed when operating the centrifuge in order to avoid the hazards of electrical shock or other physical injury.

The centrifuge is not to be used in a Class 1, Division 1, Group C hazardous location defined by the National Fire Protection Association, 8ulletin No. 56A (Inhalation Anesthetics), as extending upward to a level of 1,5 meters ( 5 feet) above the floor where flammable anesthetics are used.

To avoid electrical shock and other hazards:

Plug the power cord only into a grounded 3-wire receptacle.

Never remove the grounding prong from the power plug,

Always unplug the power cord before attempting to service the centrifuge.

El Immediately replace a worn or damaged power cord or plug,

!J Never attempt to override the safety interlock systems of the centrifuge.

1. MAINTENANCE AND SERVICE

2. Maintenance i

Routine maintenance and inspection requirements to keep the centrifuge in proper operatir~g condition are described below.

a. Lubrication

Centrifuge motors have permanently lubricated bearings. Lubrication i s not required for the life of the machine.

b. cleaning

Clean the scale plate, carrying tray, magnifier, head cover and lid with soap or a mild detergent and water. Wipe the magnifier dry with a soft tissue or cloth to avoid scratching its polished surfaces. Do not use solvents or strong, abrasive cleaners on the centrifuge or magnifier.

c. Replacing Rubber Liners e. Inspection of Motor Brushes

( 1 ) Carrying Tray and Head Outer Rim Liners Rim liners in the AUTOCRIT II Carrying Tray (Figure 8) and 24- and 16-place heads of the ADAMS Micro-Hematocrit I I Centrifuge will eventually become permanently indented from the forces exerted by the capillary tubes, When this occurs, shift the indented liner a few millimeters from i t s original position in the groove. When the entire inner surface of the liner becomes indented, remove it from the groove, reverse and re-install the liner. When both surfaces become indented, replace the liner. One spare liner i s supplied with each carrying tray and head.

RUBBER HUB

- . <

Figure 8. Carrying Tray, Showing Hub Liner Partially Removed.

(2) Nub Liner (A UTOCRIT It Centrifuge Carrying Tray Only) The rubber liner that holds the capillary tubes in the hub of the AUTOCR IT I! Centrifuge carrying tray may eventually become permanently deformed. If this occurs, the hub liner should be re- placed with a Catalog No. 0580-609-000 Hub Liner, The liner is exposed by removing the three screws, index pin and plate (see Figure 8). To remove the hub liner, free one end with the aid of a small pointed instrument, such as a blood lancet, and pull the liner out. One spare hub liner is supplied with each carrying tray.

d. Replacing Lid Seal Gasket

After prolonged use, the gasket in the upper groove of the centrifuge housing (Figuce 4) will become permanently indented by the lip of the safety liner on the lid, causing the gasket to lose i t s sealing properties. The gasket should then be replaced and a new gasket (Catalog No. 0558- 61 1-000) installed foltowing the procedure below.

Remove the old gasket by inserting a pointed instrument under the gasket and prying it out of the groove. Apply a bead of PERMABOND White Label Gasket Seal or equivalent adhesive in the bottom of the groove before installing the replacement gasket. Be sure the gasket is pushed firmly and fully down into the groove.

Motor brushes should be inspected for wear v e r y six months and replaced when worn to a length of approximately; 5 mm or 3/16 inches. Use onty the spare replacement brushes supplied !with the centrifuge or in Clay Adams Catalog No. 0551 -600-001 Motor Brush Kits.

Refer to Appendix A, DISASSEMBLY PROCEDURES, for detailed brush inspection and replacement instructions. I

f. Transporting the Centrifuge 1

Though the centrifuge can withstand the rigors of normal laboratory use, it can be damaged by dropping or by abuse in handling.

'

I f the centrifuge must be shipped, ren~ove the complete head assembly (and magnifier) before packaging. NOTE: Failure to remove the head components before shipment may result in permanent bending of the motor shaft.

Package the centrifuge in a strong, shock-proof container to prevent damage from vibration and inpact.

3. Service

a. Parts Replacement

Detailed instructions and illustrations covering replacement of the motor and spindle assembly, latch assembly, timer and other components of the AUTOCRIT I I and ADAMS Micro-Hematocrit 1;l Centrifuges are con- tained in Appendix A - DISASSEMBLY PROCEDURES, Parts should br! replaced only by trained service personnel.

b. Spare Parts

Catalog numbers of spare parts and accessories for the AUTOCR IT 11 and ADAMS Micro-Hematocrit II Centrifuges are listed in Appendix B. Most parts can be obtained from the nearest Clay Adams Dealer.

Ill. ADAMS MICRO-HEMATOCRIT II Centrifuge (Models 0556,0565,0557, and 0566)

A. USE

The ADAMS Micro-Hematocrit I I Centrifuge (Figure 9) is a high speed instru- ment designed to centrifuge capillary tubes of whole blood specimens for the quantitative measurement of hematocrits. Hematocrits of centrifuged capillary tubes must be read on a separate measuring device.

Figure 9. ADAMS Micro-Hematocrir I1 Centrifuge and Hoad Components.

Ai l modets of the ADAMS Micro-Hematocrit I I Centrifuge are identical with the exception of their power ratings (120 or 220 volts), and the following head calsac i t ies and configurations:

O Models 0556 24-Place Head (Figure 10) for accommodating 60 mm to 1120 volt) 75 mrn capillary tubes. Slots in the head are numbered from and 0565 1 to 24. (220 volt) :

Models 0567 16-Place Combination Head (Figure 11) for accommodating (120 volt) eight 75mm or 60mm capillary tubes, pluseight 75mm x 8mm and 0566 diameter serum tubes or eight MICROTAINER Capillary Blood (220 volt) : Serum Separators. Slots in the head are numbered from 1 to 16.

NOTE : Both heads are interchangeable between centrifuge models and may also be used in the AUTOCR IT tl Centrifuge (see Section I I-B), I f installed in t h e AUTOCRIT I I Centrifuge in place of the standard scale plate and carrying t r a y , the instrument can not be used for direct reading of hematocrits in the centrifuge head,

14

I

Figure 10. 24-Place centrifuge Head. I

Figure 1 1. 16-elace Centrifuge Head.

B. INSTALLATION PROCEDURES

After unpackaging, place the centrifuge dn a steady, level working surface. The head and head cover are packaged separately and are installed on the centrifuge as described below. NOTE: Use the spanner wrench which is secured to one of the bottom feet to tighten the safety locknut on the hepd and to remove the head cover. I

1. Head and Head Cover Installation ! Pull the latch handle forward to open the centrifuge lid. Unscrew the locknut from the threaded motor spindle. NOTE: The spindle is permanently secured to the motor shaft and cannot be removed.

Place the hcad over the spindle and turn it by hand untilithe two holes in the bottom of the head line up with the two pins on the spindle. Thread the locknut on the spindle and tighten i t securely but not excessively with the spanner wrench as shown in Figure 12,

Figure 12. Securing Head with Locknut and ~penne). Wrench.

i I5

Before centrifuging capillary tubes, the head cover must be placed over the head and tightened on the spindle by hand-tightening the knurled knob on the cover onto the spindle, Do not tighten the cover with the spanner wrench. NOTE: When the head cover i s not in use, place it inside the safety liner of the lid, This will prevent the lid from being closed without first installing the head cover.

2. Spare Parts

One spare rim liner for the head and one spare set of motor brushes are supplied q

with the centrifuge. The brushes are packaged in a c\oth bag, secured inside the bottom cover of the centrifuge. Brush and liner replacement procedures are described under Subsection I I- I , MAINTENANCE AND SERVICE.

3. Conversion to Direct-Reading AUTOCR IT II Centrifuge

The ADAMS Micro-Hematocrit I 1 Centrifuge can be converted to the direct- reading AUTOCR IT I I Centrifuge with a Catalog No, 0567 Conversion Kit, as described below. The Conversion Kit contains the scale plate, carrying tray, head cover and magnifier, supplied as standard equipment with the AUTOCRIT I1 Centrifuge (see Figure 4).

To install the scale plate, carrying tray and head cover on the ADAMS Micro- Hematocrit I I Centrifuge, follow the lNSTAtLATlON PROCEDURES in Subsection 11-8.

To install the magnifier, remove the pressure-sensitive cap (see Figure 9) covering the hole in the top right deck of the centrifuge housing and insert the post of the magnifier into the hole. Once the centrifuge has been converted, foI1r)w the instructions in Section I I on use of the instrumant in obtaining clirect micro-hematocrit readings.

4, Power Requirements

Connect the power cord only to a 3-wire grounded receptacle, rated as follows: Models 0556 and 0557 Centrifuges: 120 volts, 60 Hz, 15 amps Models 0565 and 0566 Centrifuges: 220 volts, 50 Hz, 7.5 amps

CAUTION

Connect only to an approved power source such as a 3-wire grounded re-ptacle. Where a 2-wire receptacle i s encountered, have it replaced with a proper! y grounded 3-wire receptacle in accordance with the National Electrical Code. Do not, undcr any circumstances, remove the grounding prong from the power plug.

$i,r,uld t k a p r e r cord &come cr-&dl frayed, broken or other- +,:% dam@: i t should be r e p l d i m d i a t ~ l y by qualified senice =e5znnd.

-=uq m v e r m x h mrcm -- 7 ~~CUIO

%. rr~e m\v xu xa~fer .-ICE am m r r q a *e r;.,;rrmm YT 3SJSSZhS,b 48r5 i:E"AZ3E%- TaZ- 2,J?,ES, L e r x A, rii a ls \Jmtra~. Fo: zsmz W ~ L W L ' ~

probierns, call Clay Adarns Customer Service Department: (201 1 887-4800.

C. PRINCIPLES OF OPERATION

Principles of operation are fully described in Subsection tl-C of this Manual. ADAMS M icro-Hematocrit I1 Centrifuges require the use of a separate measuring device to read hematocrits.

0. PERFORMANCE CHARACTERISTICS AND SPECIFICATIONS

Performance and specifications of the ADAMS Micro-Hematocrit I I Centrifuges are listed in Subsection ll-D.

I

E. OPERATING INSTRUCTIONS

1. Filling Capiliary Tubes

Capillary tubes must be filled and sealed in accordance with the blood collecting methods of Section IV - SPECIMEN COLLECTION, PREPARATION AND STAB I LITY.

2. Operating the ADAMS Micro-Hematocri~ I! Centrifuge

a. Connect the power cord to the specified 3-wire grounded AC receptacle.

b. Place the seated capillary tubes in the numbered head 'slots, keeping their sealed ends toward the periphery and against the rubber rim liner. Arrange the tubes in a balanced array.

c. Spin the head by hand to check for proper seating and position of tubes.

d. Install the head cover and tighten the cover knob by hand. Do not tighten with the spanner wrench.

e. Close the centrifuge lid and press down until the latch is fully engaged. The centrifuge will not operate unless the lid is closed.

f . Turn theTlMER dial clockwise to the desired setting.

g, Press the PUSH TO START Button in the center of the TIMER. The centrifuge head wil l immediately accelerate and the ON lamp will go 06 and wil l remain on until the centrifugation is complete and the tid lock is disengaged. NOTE : Once the TIMER is set, it does not require re-setting for subsequent centrifugations calling for the same spinning time.

h . After the ON lamp goes off and the head sfows, pull the latch handle forward to open the lid,

i i. Remove the head cover by holding the knurled knob'with one hand and

rotating the head aswrnhly clockwist! with thc other hand. If tho t:ovt:r i!, too tight to be loosened by hand, use the spanner wrench.

The centrifuged capillary tubes are now ready for readin,g on a mechanical measuring device, such as an ADAMS Micro-Hematocrit qeader, Catalog No. 1000 (Figure 131 or SPI RACR IT* Micro-Hematocrit Tube eeader, Catalog No. 100 1 (Figure 14). I

'SPIRACRI T i s a trademark o f Brunswick Corporation.

i L L 1

Figure t 3. A DAMS Micro-Hematocrit Figure 14. SPlRACRlT Reader. Micro-~emarocrit Tube Reader.

F. CALIBRATlON PROCEDURES

Refer to Subsection Il-F, CALIBRATION PROCEDURES, for instructions on speed and timer checks for the ADAMS Micro-Hematocrit I I Centrifuge.

G, OPERATING PRECAUTIONS

Carefully read and observe all of the precautions described in Subsection I l-G, OPERATING PRECUAT! ONS, before using the centrifuge.

H. HAZARDS

Consu It Subsection I I-H, HAZARDS, before using the centrifuge.

1 . MAINTENANCE AND SERVICE

Consult Subsection I!-I for warranty information and for maintenance and service procedures applicable to the ADAMS Micro-Hematocrit I1 Centrifuge.

IV. SPECIMEN COLLECTION, PREPARATION, AND STABILITY Procedures for filling and handling capillary tubes for centrifugation in the AUTOC R lT I I and ADAMS Micro-Hematocrit I I Centrifuges are described below. Whole blood specimens for micro-hematocrit determinations can be obtained from finger punctures or venipunctures.

e

A. FINGER PUNCTURE BLOOD

To f i l l the capillary tube with blood from a finger puncture:

1. Clean the finger collection area with alcohol and puncture the skin with a REDI-LANCE Disposable Blood Lancet, or equivalent, to produce a free flow of blood.

2. Grasp the end of a 75 mrn heparinized capillary tube nearest the colored ring between the thumb and forefinger.

3. Insert the other end of the capillary tube into the drop of finger blood. THE FINGER SHOULD NEVER BE SQUEEZED TlGHTLY TO INCREASE BLOOD FLOW. Tight squeezing tends to dilute the blood specimen with tissue fluid, causing a fatse low hematocrit.

4. Fill the capillary tube between 112 and 314 full as shown in figure 15. NOTE: The rate of filling may be accelerated or retarded by tilting the capillary tube (see F igu re 16).

5. When properly filled, remove the tube from the finger, holding the tube level so that blood cannot flow out of the end, NOTE: In the event the tube has been over-filled, remove the excess blood by gently dabbing the wet tip with a lint- free wiper.

6. TILT THE CAPILLARY TUBE UNTIL BLOOD FLOWS ABOUT 3 MM OR 1/8" IN FROM THE BLOOD COtLECTlNG TIP (see Figure 17). Return the capillary tube to a horizontal position so that no blood is lost.

Colored Ring

- -

Figure 15. Capillary Tube, Showing Fill Level Region.

Figure 16. Tilting Capiliary Tube to Figure 1 7. Tilting Tube to Remove Regulate Blood Flow. Blood From Tip.

7. Wipe any droplets of blood from the outside surface of the capillary tube using a lint-free wiper.

8. SEAL THE CAPILLARY TUBE AS FOLLOWS:

a, Invert the capillary tube so that the dry end not used for blood collecting points down.

b. IMMED~ATELY lNSERT THE BLOOD-FREE, DRY END INTO SEAL- EASE Tube Sealer, as shown in Figure 18. Push the tu'be fully down into the clay before blood flows into the dry end. THE END OF THE TUBE MUST BE DRY FOR THE CLAY TO SEAL. I

NOTE: If Blood reaches the end of the tube before the SEAL-EASE Tube Sealer i s forced in, some blood may be lost and the clay will not seal.

The tube i s now ready for centrifugation.

Figure 18. Seeling Dry End of Tube in SEA L-EASE Sealing Clay.

Figure 19. Regulating Blood Flow From Test Tube to Capillary Tube.

B. VENOUS BLOOD

Blood from venipunctures can be collected with a standard syringe or a VACU- TAlN ER Brand Blood Collection Tube. To prepare venous blood for a hemato- c r i t determination:

1. Mix the blood thoroughly by inverting t hc VACUTAlNER Brand Blood Collection Tube or syringe several times. Remove the adapter from the VACU- TAINER Brand Blood Collection Tube and discard the needle. I f blood is collected with a syringe, transfer the blood to a clean test tube.

2. Grasp one end of the capillary'tube near the colored ring between the thumb and forefinger.

3. Tilt the VACUTAlNER Brand Blood Collection Tube or test tube to a near horizontal position, so that blood flows toward the mouth o f the tube.

4. Dip the end of the capillary tube into the blood specimen. Hold the tube in a horizontal position tilting the end, if necessary, to regulate the flow of blood {see Figure 19).

5. Fill the capillary'tube to at least half- to three-quarters full and seal the tube following Steps 4-8 of Paragraph A, FINGER PUNCTURE BLOOD.

Y

C. INTERFERING SUBSTANCES

1, Anticoagulants

It has been reported that the hematocrit value of blood anticoagulated with oxalates is 8% to 13% less than that obtained with heparin.(41*151 It is also reported that reliable hematocrit values cannot be obtained when the concen tration of disodium ethylenediaminetetraacetate (EDTA) anticoagulant exceeds 2 mg per ml of whole blood.(7)

2. Drugs

Certain drugs will cause variations in hematocrit levels due t o physiological factors. Consult Reference ( 5 ) for detailed information.

D. STORAGE

If the blood specimen has been anticoagulated to prevent, clotting, the filled capillary tubes can be stored in the SEAL-EASE Tube Holder for up to 90 minutes prior to centrifugation. ,

. .

A. MATERIALS REQUIRED, BUT NOT PROVIDED I

The following materials required to perform micro-hematocrits are not provided with the AUTOCR IT 11 or ADAMS Micro-Hematocrit I I Centrifuge:

D 75 mm Capillary Tubes - Clay Adams YANKEE Micro-Hematocrit tubes (Catalog No. 1020), 60 mm Capillary Tubes, or equivalent.

Capillary Tube Sealer - Clay Adams SEAL-EASE TubeSealer (Cataiog No. 10 15), or equivalent.

C1 Lint-free Wipers

Mechanical Reading Device (for use with the ADAMS Micro-Hematocrit II Centrifuge only) - ADAMS Micro-Hematocrit Reader (Catalog No. 1000), SPlRACR IT Micro-Hctmatocrit Reader (Catalog No, 1001), or equivrrlct~t.

B. USlNG THE AUTOCRlT !I Centrifuge

1. Fill and seal capillary tubes in accordance with Section IV of this Manual.

2. Load the carrying tray of the centrifuge in a balanced array, as described in AUTOCRIT II Centrifuge Operating Instructions, Be sure that the sealed ends face the outer rim of the tray.

3. Record the tray identification code and slot number of each patient specimen.

4. Centrifuge the capillary tubes for at least three minutes and read the hemato- crits directly on the Centrifuge scale plate, as described' in Subsection I l -E, AUTOCRIT II Centrifuge Operating Instructions, 1

C. USING THE ADAMS Micro-Hematocrit II Centrifuge

1. Fill and seal the capiltary tubes in accordance with Section IV of this Manual,

2. Load the head of the centrifuge in a balanced array as described in the ADAMS Micro-Hematocrit i l Centrifuge Operating Instructions, Subsection I ll-E. Be sure that the sealed ends face the outer rim of the head.

3. Record the correct slot number of each patient specimen:

4. Centrifuge the capillary tubes for at least three minutes; remove the tubes after centrifugation.

5, Read the hematocrits on a separate measuring device, carefully following the instructions supplied by the manufacturer.

.- - .--- - - .----

V!. TEST RESULTS AND LlMlTATlONS APPENDIX A P

The AUTOCA IT I I Centrifuge incorporates an integrai micro-hematocri t scale against which a direct quantitative reading of the hematocrit can be made. Additional computations are not required by the operator.

The ADAMS Micro-Hematocrit II Centrifuge requires a separate mechanical reading device to measure the hematocrit. Consult the instructions of the manu- facturer before using the reading device.

See Subsection I-C for details on micro-methods for calculating the hematocrit.

V11. EXPECTED VALUES Normal hematocrit levels for healthy adult males are 40% to 54%(6).

Normal hematocrit levels for healthy adult females are 37% to 47%(6).,

DISASSEMBLY AND REPLACEMENT PROCE~URES (AUTOCR IT I1 and ADAMS Micro-Hematocrit 11 Cyntrifuges)

CAUTION: i UNPLUG THE POWER CORD BEFORE DISASSEMBLING THE CENTRIFUGE. REPLACEMENTS SHOULD BE MADE ONLY BY :TRAINED SERVICE PERSONNEL.

- --

I. Inspection and Replacement of Motor Brushes

Motor brushes in the AUTOCRIT II and ADAMS Micro-Hematocrit II Centri- fuges should be inspected for wear every six months. Brushes should be replaced when worn to approximately 5 rnm or 3/16". Replace brushes only with the spare set of brushes supplied with the centrifuge or with the correct Clay Adams Motor Brush Kit specified on the label affixed to the motor housing.

Vl I I. SPECIFIC PERFORMANCE CHARACTER~STICS To inspect and replace the motor brushes: Micro methods for the determination of hematocrits have been widely used

t

in laboratories for many years. The results of micro-hematocrit determina- a. DISCONNECT THE POWER CORD.

tions are recognized as being fully equivalent to the results of macro deter- b. Remove the bottom cover assembly (see Figure 201 by removing the

m i n a t i o n ~ . ( ~ ) l ( ~ ) three screw and lockwashers (Item A) that hold the cover to the mounting plate posts. DO NOT REMOVE THE RUBBER FEET. :

c. Unscrew the brush caps (I tern D) from either side of the motor housing. I f the brush contact plate does not spring out when the c a p h removed, care- fully pry the contact plate out using a small screwdrive*.

d. Remove the brushes by pulling the brush springs out.

e. inspect the brushes and replace them with new brushes if worn to a length of 5 mm or 3116". NOTE: A spare set of brushes is packaged in a cloth bag secured by a cable tie to one of the motor plate posts.

PRlP

Figure 20. t

Schematic, AUTOCRIT 11 and ADA MS Micro. Hemaprit I1 Cent t

'ri fuges.

f . To replace the original brushes or to install new brushes, orient each brush so that the curved surface of the brush matches the curvature of the motor housing as shown in Figure 21.

g. Re-install the brush caps and bottom cover. NOTE: New brushes may require wearing in before the automatic motor brake will operate. Brushes will normally wear in after about 30 minutes of running time.

Figure 2 1. Correct Orion ti) tion of Mo tor Brusl~ lor Replacemon t.

2. Motor and Spindle Assembly (M2)

She replacement Motor and Spindle Assembly (Catalog No. 0558.600-000) for 120 volt units only is supplied in one of three different but interchangeable configurations. A non-interchangeable drip plate is included with the replace- ment Motor and Spindle. The old drip plate should be discarded and the new drip plate used with the replacement Motor and Spindle.

When installing a replacement 220 volt Motor and Spindle Assembly (Catalog No. 0562-600.000), the drip plate should not be disassembled from the bottom cover and should be re-used.

Rer~lacement procedures are described below.

3 . DISCONNECT THE POWER CORD.

I-). Remove the complete head assembly from the motor spindle, close the lid, and turn the centrifuge over.

c. Remove the bottom cover by removing the three screws and lockwashers that hold the cover to the mounting plate posts. Unscrew the four rubber feet that secure the drip plate (see Figure 20) to the bottom cover.

NOTE: In 220 volt units, do not disassemble the drip plate from the bot- tom cover.

( I . Discard the drip plate (except in 220 volt units).

e. Referring to the Wiring Diagram in Figure 22 and lniernal View in Figure 23, remove the Tyrap from 'the motor wire harness, and disconnect the term inal connectors of the following motor wires: ,

Black wire from Latch and Solenoid Switch S3-8 (COM) White wire from Latch and Solenoid Switch S3-B (NO) Red wire from Latch and Solenoid Switch S3-A (COM) Yellow wire from Position 1 on the Main Terminal Block Slue wire from Position 6 on the Main Terminal Block

Figure 22. Wiring Diagram, AUTOCRIT I1 and ADAMS ~ i c r o - ~ e m a t o c r i t / I Centrifuges.

f. Turn the centrifuge over so that i t rests on the three mounting plate posts. Open the lid.

9. Supporting the motor from unrlerneath with one hand, remove tho fotrr screws and lockwashers ( I tern 8 in Figure 20) from !the motor mounting plate in the top of the centrifuge. The motor and spindle assembly can be dropped through the mounting plate and removed through the bottom of the centrifuge.

h. Install the replacement motor and spindle assembly $nd new drip plate by reversing the disassembly procedure. Install a Tyrap or tape around the motor wires. I

S A F E T Y S W I l PLATE-

3. Timer (MI )

a. DlSCONNECT THE POWER CORD.

SWITCH d1.A LATCH AND SOLENOLO S3.A ASSEMBLY

b. Peel of f the pressure sensitive plate from the front of the timer.

d

c . Remove the two Phillips-head screws underneath the pressure sensitive piate that mount the timer to the housing.

1

Figure 23. Internal View, AUTOCRl T I / and Micro-Hemstocrit I I Centrifuges.

t l . Turn the centrifuge over and remove the bottom cover by removing the screws and lockwashers that hold the cover to the mounting plate posts. DO NOT R E ~ O V E THE R U B B E R FEET.

e. Pull the timer out and remove the mounting screw that secures the terminat ring of the green ground wire to the timer plate.

f . Disconnect all terminal connectors of the front panel harness wires from timer Switches S1-A and S1-I3 (see Figure 23).

g. Disconnect the terminal connectors of the black timer wire from Position 7 on the main terminal block and the white timer wire from Position 1 on the main terminal block. Remove the timer.

h. lnstall the replacement timer by reversing the disassembly procedure. NOTE: Replacement timers are supplied with a new adhesive-backed name plate for installation over the timer dial.

i

WARNING:

FOR CONTINUED OPERATOR PROTECTlON, E SURE TO fi RE-CONNECT THE TERMINAL RING OF THE GREEN GROUND WIRE TO THE TIMER PLATE.

I i

4. ON Lamp (Dl) a. DISCONNECT THE POWER CORD.

b. Remove the bottom cover by removing the three xrews and lockwashers that hold the cover to the mounting plate posts. 00 NOT REMOVE THE RUBBER FEET.

c. From inside the centrifuge, iocate the ON lamp Dl next to the timer and cut the snap-on speednut that holds the ON lamp in the housing.

d. Disconnect the terminal connectors of the two black lamp wires from Positions 1 and 7 on the main terminal block.

e. Pu!l the ON lamp and wire leads through the front of the centrifuge housing.

f. Install the rcptacement ON tamp by reversing the disassembly procwlurc. Note: A snap-on speednut is supplied with each replacement ON liltnp,

5. Latch and Solenoid Assembly

a. Perform Steps a and b of Replacement Procedure 4.

b. Disconnect the wire terminal connectors from the solenoid and from switches S3-A and S3-6.

c. Remove the screw, hex nut and washers that hold the terminal ring of the green ground wire to the latch and solenoid bracket.

d. Referring to Figure 20, remove the .four xrews, lockwashers and flat washers (Item C) that mount the latch'and solenoid assembly to the inside of the housing.

e. Turn the centrifuge over, open the lid, and remove the latch and solenoid assemt~ly through the top of the centrifuge.

f. Insert the replacement latch and solenoid assembly through the top of the centrifuge. Close the lid and turn the centrifuge over or on its side,

g. Replace the four flat washers, lockwashers and screws that hold the latch and solenoid assembly to the housing. DO NOT TIGHTEN THE SCREWS SECURELY.

h. Referring to Figure 20, check the clearance between: the latch in the fully extended position and the lid safety liner. The latch assembly is correctly positioned when this clearance is 6.3 mrn (0.250 in.). Adjust, as necessary, and tighten the screws.

i . Perform the remaining re-assembly of the latch and solenoid assembly in reverse order.

WARNING:

FOR CONTINUED OPERATOR PROTECTION, BE SURE TO RE-CONNECT THE TERMINAL RING OF THE GREEN GROUND W I R E TO THE SOLENOID BRACKET.

6. Lid Safety Switch (S2)

a. Perform Steps a and b of Replacement Procedure 4.

b. Remove the two mounting screws and lockwashers that hold the safety switch (S2) plate (see Figure 23) to the upper housing, and remove the switch and switch plate.

c , Disconnect the terminal connectors on the white wire and whitelblue wire from the safety switch.

d. Depress the snaps on either side of the safety switch and push the switch through the hole in the mounting plate.

e. Snap the replacement safety switch into the mounting plate and install the switch and plate in reverse order.

7. Varistor

A varistor (see Figure 23) i s connected across the input voltage line on the main terminal block. To replace the varistor:

a. Perform Steps a and b of Replacement Procedure 4.

b. Unsotder the two leads of the varistor from Positions 1 and 2 on the main terminal block.

c. Install the replacement varistor by reversing the disassembly procedure.

8. Linecord and Plate Assembly

a. Perform Steps a and b of Replacement Procedure 4.

b. Referring to Figure 23, remove the screw and lockwasher that secure the I inecord plate to the mounting plate assembly and housing.

c. Slide the linecord plate, Heyco bushing and linecord out of the housing.

d, Disconnect the terminal connectors of the white and black linecord wires from Positions 1 and 2 of the main terminal board.

e. Remove the screw and two washers that secure the ground terminal ring of the green lhecord wire to the motor mounting plate assembly. Remove the Tyrap holding the linecord to the motor mounting plate.

f. Remove the linecord and plate assembly and install the replacement assembly in reverse order. Instal! a Tyrap or tape around the linecord.

WARNING:

FOR CONTINUED OPERATOR PROTECTION, RECONNECT THE GREEN GROUND WIRE TO THE MOUNTING PLATE WHEN INSTALLING THE REPLACEMENT LINECORD*

9. Lid and Hinge Block Assemblies

The lid and hinge block assemblies must be removed and replaced as a single unit as follows:

a. Removal

( I} Perform Steps a and b of Replacement Procedure 4.

(2) Locate the two hex nuts inside the upper back of the housing, between the housing and mounting plate assembly. Using a 5/8" nut driver, remove the two hex nuts, lockwashers and f latwashers.

(31 Carefully turn the centrifuge over, pull the 'latch handle forward, and remove the lid and hinge block.

b. Replacement I

( I ) Carefully orient the new lid and hinge block in 'position on the top of the centrifuge. I

(2) Holding the lid with one hand, turn the centrifude over and re-install the two flat washers, lockwashers and hex nuts tbat secure the hinge block to the housing. DO NOT TIGHTEN THE HEX NUTS,

(3) Turn the centrifuge over and, referring to FiQure 24, adjust the position of the lid and hinge block to obtain the correct gaps between the lid and the housing. Gaps A and 0 should be approximately quai; Gap C should be 2.38k0.38 mrn (3/321164 inches).

(4) When the gaps are adjusted correctly, carefully tighten the two hex nuts into the hinbe block.

(5) Re-assemble the centrifuge in reverse order.

Figure 24. Lid and Housing Detail, Showing Correct Gap Adjustments.

APPENDtX B SPARE PARTS, HEAD COMPONENTS AND ACCESSORIES (AUTOCRIT I I and ADAMS Micro-Hematocrit I I Centrifuges)

1 turn No. Iseo Figure 251 Catalog NO. 12. L id and Hinge Block Asurmbly 0558.604-000

1. Motor and Spindle Assembly (with O r ~ p Plate] - 120 Vo l l Centrifuges

Lid Dowel Pin

(Rare Model No. 0558) 0558-600900 Hinge Block Motor Screw, Hex Hd., 1 14-20 x 518 LG Sp~ndle Cad. Plt. Stl. (21 Screw, Rd. Hd., Philllps k8-32 x Washer, Flat 114 I.D. St. Stl. I2)

51 16 kg. ( 4 ) Washer, Split Lock, 114 I.D. St. St!. 121 Warher. Ext.. $8 (41

13. Rubber Feet 0558-606-000 2 Motor and Spindle Assembly - (Package of 41

220 Volt Centrifuges (Base Model No. 0562) 0562-600-000 14. Spanner Wrench Assembly 0551.608-000 Violor Sp~ndle Spanner Wrench

Screw. Rd, Hd.. Phillips #8.32 x Chain Ring [Large)

5/16 ig. 141 Bead Chain Washer. Ext.. =i8 141 Chain Ring ISmalll

3 Motor Brush K i t - See Motor Label . 15. L id Seal Gasket 0558-61 1 4 0 0

for Roplacemant Pert NO. 16. Safety Locknut 0551.600-002

.4. Tlmcr and Name Plate (120 Volt) 17. Varistor (120 Volt) Purchase 0558-601 -000 150 V, G.E. PIN 150LAl OA Locally

T~rner Screw, FH ~hil l ips82', #8 X 5/16,

Type A. Stainless (21 Push.On Connecton, Attached (2) Name Plate

5 . Timer and Name Plats 1220 Volt) . 0562.601 4 0 0

Timer Screw, F H Phillips 82', #8 x 5/16,

Type A. Stainless (2) P~r.;lr On Connectors, Attached 121 N , ~ i ~ l t * l ' \ n r t ~ !

6. ON Lomp Assembly (1 20 Volt) 0558-615-000

Spcednur ~ i , s , h 1311 Corrncclor. Artachod (21

7. ON Lomp Assornbly (220 Volt) 0562-61 5.000

s ~ ~ l ~ ~ ! l l f ~ ~ l ~ push 01, Connector, Attoclwd (21

8 . Latch. Solenoid and Switch Auemblv (120 Volt) 0558.6 10.000 Lillcll Solenoid Urilckut Swllchvs 121 Scrl!w (41 tockwa~her (41 Flat Washer (4)

9, Larch, Solenoid and Switch Assembly 1220 Vol t l 0562.610-000 Latch 1

Solenoid Bracket Switches l 2 I Screw I41 iockwasher (4) r la1 W;~shc:r i41

10. Lid Safety Switch 0558602-000

11. Linecord and Plate Assembly 0558-603-000

Linecord H e ~ c o Bushing Pl,rtL? (Connectors Attached)

18. Varistor 1220 Volt) Purchase 150 V, G.E. PIN 250LA15A Lpcatly

Head Components: AUTOCRIT 11 Centrifuge

19. Scale Plate Assembly 0577-605-000

20, Rubber Liner, Carrying Tray 0580-607-000

21. Guide pin; Carrying Tray 0580-608.000

22. Scrow. Huh Plate 131' - Flat He;jrl Ptldlips No. 2-56 M 112

23. Notchud Ruhbor Liner, Carrying Tray 0680-609-000

24. Head Cover Assembly 0574-605-000

26. Mngnifior 0558-6 12-000

Head Components: ADAMS Micro.Homatocrit II Contriluge

26. Hoed Linor. Rubber Oti51.606~001 (3 linurs par p t ~ c k n ~ o l

27. Head Covar Assembly 0551-605.000

Conversion Ki t Scala Ploro. Carrying Troy. Hood 0567 Cover. Magnifier. Name Plate

Accessories Catalog No.

YANKEE Micro.Harnarocrit Tubes 175 mm) Heparinized 1020 Plain 1021

Capillary f ubes (32) Heparinized 1022 Plain 1023 Double Oxalated 1024 Serum Tubes 18 x 75 mrn)

or use with Cat. No. 0564 Head 1026

SEAL-EASE Capiilary Tube Sealer 1015

READI.LANCE Blood Loncels 5302

ADAMS Micro-Hematocrit Reader 1000

SPlRACR IT Micro-Hematocrit Tube Reader 1001

'Attaching hardware no1 supplied. Purchase Locally.

Figure 25. Exploded Isometric, AUTOCRlT / I and Micro-Hematocrit I1 Centrifuges.

3 1