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PESTICIDE RESIDUE IN BANGLADESH TEA Bangladesh Tea Research Institute at Srimangal has established a well-equipped sophisticated pesticide residue analytical laboratory at its own premise and is run by trained personnel since January 2003. All sorts of precision analyses of international standard of different groups of pesticides like Organochlorine, Organophosphate, Carbamate, synthetic Pyrethroid and some unclassified groups are done successfully. Standards of Good Agricultural Practices (GAP) and Good Laboratory Practices (GLP) are strictly followed. The facilities are now open to the tea industry and they have already started deriving benefit out of it. So the producers and traders should start scrutinizing their teas on regular basis at field, factory and trade points without delay. They should contact BTRI and harness the full advantage of the available facilities. The laboratory has already been selected as one of the 10 laboratory by Intergovernmental Group (IGG) of FAO, Rome, Italy for ring tests to fix MRL in black tea globally. Pesticide Residue Pesticide residue in made tea means what is left over or remains of a pesticide in the made tea after the application of a pesticide to tea bushes in the field prior to plucking and subsequent manufacturing. As tea is a consumable commodity, the effect of residue of pesticides in made tea is harmful to human health. Tea produced in Bangladesh is exported to different countries of the world particularly West and East European as well as Middle Eastern countries. The consumers of these countries have become more conscious about the residue of the pesticides. In this perspective, EPA/Codex Commission/FAO/WHO, German Law etc. have given restrictions on producing and procuring tea having pesticide residue. Objectives 1. Development of protocols on pesticide formulation and residue analysis. 2. Testing the purity of marketed brands of pesticides. 3. To find out whether any residues remain in the crop at harvest and subsequent processing as finished consumable product causing any toxic hazard to the consumers home and abroad. 4. Comparison of observed residue level of a particular pesticide with FAO/WHO recommended Maximum Residue Limit (MRL) and disseminating this information to the policy planners, planters and tea management personnel. Capability BTRI Pesticide Residue Analytical Laboratory is equipped with Gas Chromatograph (Shimadzu GC-14B) with other necessary equipment. GC-14B Two Gas Chromatographs are equipped with following detectors: Electron Captured Detector (ECD) Flame Ionization Detector (FID) Flame Thermoionic Detector (FTD Services Testing the purity of commercial pesticides. Providing residue analytical report on exportable tea for exporter and local entrepreneurs. Future Plan Establishing the Pesticide Residue Analytical Laboratory as reference laboratory globally. Achievements Good Laboratory Practice (GLP) is introduced. Protocols for the residue analysis of Endosulfan, Cypermethrin, Fenvalerate, Propargite, Ethion, Malathion, Dimethoate, Bifenthrin and Sulphur are developed. Purity testing of commercial pesticide is available. Protocol for multi-residue analysis is developed. Basic requirements for producing residue free tea Vigilance of pest species Selection of pesticides Biological information of the pest Dilution of the pesticides Correct dose of pesticides Method of application Avoidance of repeated rounds of spraying Shelf life of pesticides Consider weather condition Consider spraying interval period How the tea industry will benefit The Residue Laboratory at BTRI is at the doorstep of the tea industry. They can easily take reliable as well as economic service within a short time which would involve more money, time and longer process to get samples tested from any other laboratory in the country or outside. At present the Institute can analyze about 200 tea samples per annum at different levels e.g. field application, made tea at factory, experimental samples, auction point, export point and random samples in the market point. The volume of analyses will be extended further. Thus the
Transcript
Page 1: PESTICIDE RESIDUE IN BANGLADESH TEAshameembtri.webs.com/Residue Leaflet.pdf · PESTICIDE RESIDUE IN BANGLADESH TEA ... Kazi & Kazi T.E.0.5 - ... Tea Estates frequently send made tea

PESTICIDE RESIDUE IN BANGLADESH TEA

Bangladesh Tea Research Institute at Srimangal has established a well-equipped sophisticated pesticide residue analytical laboratory at its own premise and is run by trained personnel since January 2003. All sorts of precision analyses of international standard of different groups of pesticides like Organochlorine, Organophosphate, Carbamate, synthetic Pyrethroid and some unclassified groups are done successfully. Standards of Good Agricultural Practices (GAP) and Good Laboratory Practices (GLP) are strictly followed. The facilities are now open to the tea industry and they have already started deriving benefit out of it. So the producers and traders should start scrutinizing their teas on regular basis at field, factory and trade points without delay. They should contact BTRI and harness the full advantage of the available facilities. The laboratory has already been selected as one of the 10 laboratory by Intergovernmental Group (IGG) of FAO, Rome, Italy for ring tests to fix MRL in black tea globally.

Pesticide Residue Pesticide residue in made tea means what is left over or remains of a pesticide in the made tea after the application of a pesticide to tea bushes in the field prior to plucking and subsequent manufacturing. As tea is a consumable commodity, the effect of residue of pesticides in made tea is harmful to human health. Tea produced in Bangladesh is exported to different countries of the world particularly West and East European as well as Middle Eastern countries. The consumers of these countries have become more conscious about the residue of the pesticides. In this perspective, EPA/Codex Commission/FAO/WHO, German Law etc. have given restrictions on producing and procuring tea having pesticide residue. Objectives 1. Development of protocols on pesticide formulation and

residue analysis. 2. Testing the purity of marketed brands of pesticides. 3. To find out whether any residues remain in the crop at

harvest and subsequent processing as finished consumable product causing any toxic hazard to the consumers home and abroad.

4. Comparison of observed residue level of a particular pesticide with FAO/WHO recommended Maximum Residue Limit (MRL) and disseminating this information to the policy planners, planters and tea management personnel.

Capability BTRI Pesticide Residue Analytical Laboratory is equipped with Gas Chromatograph (Shimadzu GC-14B) with other necessary equipment. GC-14B

Two Gas Chromatographs are equipped with following detectors: Electron Captured Detector (ECD) Flame Ionization Detector (FID) Flame Thermoionic Detector (FTD Services Testing the purity of commercial pesticides. Providing residue analytical report on exportable tea for

exporter and local entrepreneurs. Future Plan Establishing the Pesticide Residue Analytical Laboratory as

reference laboratory globally. Achievements Good Laboratory Practice (GLP) is introduced. Protocols for the residue analysis of Endosulfan,

Cypermethrin, Fenvalerate, Propargite, Ethion, Malathion, Dimethoate, Bifenthrin and Sulphur are developed.

Purity testing of commercial pesticide is available. Protocol for multi-residue analysis is developed.

Basic requirements for producing residue free tea Vigilance of pest species Selection of pesticides Biological information of the pest Dilution of the pesticides Correct dose of pesticides Method of application Avoidance of repeated rounds of spraying Shelf life of pesticides Consider weather condition Consider spraying interval period How the tea industry will benefit The Residue Laboratory at BTRI is at the doorstep of the tea industry. They can easily take reliable as well as economic service within a short time which would involve more money, time and longer process to get samples tested from any other laboratory in the country or outside. At present the Institute can analyze about 200 tea samples per annum at different levels e.g. field application, made tea at factory, experimental samples, auction point, export point and random samples in the market point. The volume of analyses will be extended further. Thus the

Page 2: PESTICIDE RESIDUE IN BANGLADESH TEAshameembtri.webs.com/Residue Leaflet.pdf · PESTICIDE RESIDUE IN BANGLADESH TEA ... Kazi & Kazi T.E.0.5 - ... Tea Estates frequently send made tea

Institute plans to ensure MRL safe tea for the consumers home and abroad. Maximum Residue Limits (MRL) of certain pesticides in tea prescribed by different countries/organizations (in ppm)

Name of the chemical

EU+ EPA JAPAN CODEX

Bifenthrin 5.0 - 5.0 - Chorpyrifos 0.1* - 10 2.0 Cypermethrin 0.5 20 20 20 Deltamethrin 5.0 - 10 5 Dicofol 20 45 3.0 50 Dimethoate 0.05* - 1.0 - Endosulfan 30 24 30 30 Ethion 3.0 10 0.3 - Fenvalerate 0.05* - - - Malathion 0.5 - 0.5 - Propargite 5.0 10 5.0 5.0 Sulphur Exempted - - -

Indicates lower limit of analytical determination; N. B. Lower the MRL value higher the toxicity level

Pesticide residue survey in Bangladesh tea Tea Estates frequently send made tea sample for pesticide residue analysis at BTRI. Among the samples so far analyzed, none exceeded the MRL fixed by the EPA, Codex Commission and EEC/EU.

Residue level of pesticides of the samples collected from different tea estates in Bangladesh.

Name of Tea Estate

Name of chemical

Residue Level (ppm)

Remarks

Rajkie T.E. Endosulfan 0.18 Below MRL Allynugger T.E. Endosulfan 0.10 Below MRL Karimpore T.E. Endosulfan 0.48 Below MRL Amo T.E. Endosulfan 0.54 Below MRL Abul Khair Grp. Endosulfan 1.00 Below MRL Seylon Tea Endosulfan 0.54 Below MRL Lungla T.E. Propargite 1.59 Below MRL Karimpore T.E. Propargite 0.00 Not detected Chatlapore T.E. Propargite 0.00 Not detected

Etah T.E. Propargite 2.44 Below MRL Shumshernugger Propargite 0.00 Not detected Ootterbagh T.E. Propargite 0.00 Not detected Duncan Brothers Propargite 1.82 Below MRL Siloah T.E. Ethion 1.98 Below MRL Chaklapunji T.E. Ethion 0.20 Below MRL Karimpore T.E. Ethion 0.96 Below MRL Chaklapunji T.E. Sulphur 0.00 Not detected Shumshernugger Sulphur 0.00 Not detected Naloa T.E. Sulphur 0.00 Not detected Luskerpore T.E. Sulphur 0.00 Not detected Mazdehee T.E. Sulphur 0.00 Not detected Amo T.E. Sulphur 0.00 Not detected Chandpore T.E. Sulphur 0.00 Not detected Kazi & Kazi T.E. Endosulfan 0.00 Not detected Kazi & Kazi T.E. Malathion 0.00 Not detected Kazi & Kazi T.E. Ethion 0.00 Not detected Kazi & Kazi T.E. Fenvalerate 0.00 Not detected Kazi & Kazi T.E. Cypermethrin 0.00 Not detected Kazi & Kazi T.E. Propargite 0.00 Not detected

BARC funded Running Project

“Determination of residue level of different pesticides at different days after spraying” Core Research Programme/Project implemented by Entomology Division, BTRI during the year of 2007-10 under “Research Grant” of BARC

Objectives of the project

1. To determine the safe harvest interval of plucking after spraying of pesticides.

2. To increase awareness on the adverse effect of non- judicious application of pesticides.

Project output A series of experiment on some commonly used pesticides were carried out at BTRI main farm. After application of pesticides at BTRI approved doses, shoots were harvested at ‘0’, 1st, 3rd, 5th, 7th, 10th & 14th day according to FAO recommendation. Leaves were plucked at respective interval and were manufactured to finished tea in the Mini Tea Factory of the Institute following CTC method. Determination was done by Electron Captured Detector (ECD) and Flame Thermoionic Detector (FTD) using Shimadzu Gas Chromatograph (GC-14B) with DB-5 capillary column (0.53 mm i.d.) and Nitrogen (99.997%) as carrier gas.

Hexane and Acetone were used as extracting reagents and Florisil as clean-up reagent.

Residues and Harvest intervals for some common chemicals used in tea plantation

Chemical Dose /ha Residues in made tea*

(ppm)

Safe harvest interval (days)

Endosulfan 1.5 lit. 1.40 7 Propargite 1.0 lit. 3.52 7 Ethion 1.0 lit. 4.66 8 Bifenthrin 500 ml 0.043 7 Cypermethrin 500 ml 0.85 7 Dimethoate 2.25 lit. ND* 7 Sulphur 2.25 Kg ND 7

* 7 days after spraying, ND-Not Detectable

The residue level of above mentioned pesticides at 7 days after plucking are below the MRL fixed by EPA, Codex Commission, EEC/EU and German Law except Ethion.

Project Personnel

Dr. Mainuddin Ahmed, CSO & Programme Leader Mr. Mohammad Shameem Al Mamun, SO Mr. Shovon Kumar Paul, SO

Contact Address Director

Bangladesh Tea Research Institute Srimangal-3210, Moulvibazar

Phone: +88 08626-71225, Fax: +88 08626-71930 Email: [email protected]

June 2010


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