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Photosynthesis and Cellular Respiration Section 2
Organisms classified on how they obtain energy
• Autotrophs– Make their own food from organic molecules– Most convert light energy into chemical energy
• Carbon Dioxide (CO2) and water (H2O) to organic compound
• Oxygen (O2) released
• Heterotrophs– Cannot make their own food inorganic compounds– Eat autotrophs or other heterotrophs
Photosynthesis and Cellular Respiration Section 2
Harvesting Light Energy
• Photosynthetic organisms have chloroplasts– Chloroplasts are the organelles that convert
light energy into chemical energy.• Contains flat, disc-like sacs called thylakoids.
• this is the first stage of photosynthesis.
Photosynthesis and Cellular Respiration Section 2
• Sunlight composed of ROY G BIV, different colors. (Lgth = Amt of Engy)
• Color observed when light strikes an object, waves are reflected, transmitted, or absorbed
• Colors react depending on the pigment present
Light Absorption in Chloroplast
Photosynthesis and Cellular Respiration Section 2
Harvesting Light Energy, continued
• Chlorophyll (a and b) – Absorbs blue and red light and reflects green
and yellow light• Carotenoids
– help plants absorb additional light energy– absorb blue and green light, reflect red,
orange and yellow(fall colors)
Photosynthesis and Cellular Respiration Section 2
Two Electron Transport Chains• First reaction in photosynthesis is the light reaction takes
place in the chlorophyll– Light hits a thylakoid– Energy is absorbed by the pigment molecules and are
directed to a special chlorophyll molecule that acts as a reaction center
– Electrons are excited and move to other molecules and the electron transport chain.
• One ETC makes ATP and Second makes NADPH
Photosynthesis and Cellular Respiration Section 2
Producing ATP
Step 1:• An enzyme splits water
molecules , e- is released.
• H+ ions stay within the thylakoid
• e- is charged by pigment• Oxygen gas is released
into the atmosphere
1st Electron Transport Chain
Photosynthesis and Cellular Respiration Section 2
Step 2: • Carrier Proteins (ATP
Synthase) in the membrane pump H+ ions into the thylakoid,
Photosynthesis and Cellular Respiration Section 2
Step 3: • Energy diffusion of H+
ions through ATP synthase is used to make ATP
Photosynthesis and Cellular Respiration Section 2
2nd Electron Transport Chain
Step 4: Producing NADPH• Renergizing, Light excites
electrons that are passed on to the second chain.
Photosynthesis and Cellular Respiration Section 2
Step 5: Making NADPH• Excited electrons
combine with H+ ions and NADP+ to form NADPH.
• NADPH is used to store energy in organic molecules. (used later in dark reaction)
Photosynthesis and Cellular Respiration Section 2
Electron Transport Chains of Photosynthesis
Click to animate the image.
Photosynthesis and Cellular Respiration Section 2
Producing Sugar
• The first two stages of photosynthesis depend directly on light to make ATP and NADPH.
• In the final stage of photosynthesis, ATP and NADPH are used to produce energy-storing sugar molecules from the carbon in carbon dioxide.
• The use of carbon dioxide to make organic compounds is called carbon dioxide fixation, or carbon fixation. (Light Independent, Dark Reaction, or Calvin Cycle)
Photosynthesis and Cellular Respiration Section 2
Calvin Cycle
• Carbon Fixation, an enzyme adds CO2 to a five carbon molecule (RuBP)
• Takes place outside of the Thylakoid in the stroma, solution surrounding the thylakoids
Photosynthesis and Cellular Respiration Section 2
Calvin Cycle• CO2 diffuses into the stroma
• The enzyme (RuBP) adds CO2 to a five carbon molecule
• This occurs 3 times to make six carbon molecule
• 6 carbon molecule splits into 2 – 3 carbon molecules (3 – PGA)
Photosynthesis and Cellular Respiration Section 2
• Each of 3 – PGA is converted into G3P– G3P is a molecule with additional phosphate and H+
that were donated by ATP and NADPH– ADP and NADP are then recycled to the ETC
• One G3P molecule leaves the Calvin Cycle to be used to make carbohydrates
• The remaining G3P molecules are recycled back to the enzyme RuBP and reenters the Calvin Cycle
Photosynthesis and Cellular Respiration Section 2
Factors that Affect Photosynthesis
• Light intensity• Carbon dioxide concentration• Temperature