Date post: | 26-Apr-2018 |
Category: |
Documents |
Upload: | nguyenduong |
View: | 216 times |
Download: | 3 times |
Project info
This project has received funding iunder the framework of JPI Oceans
Project logo
Project info
Acknowledgement of relevant funding agencies. Please find all logo’s on the following website:
https://epss-jpi-oceans.ptj.de/partners
Project logo
PLASTOX is supported by national funding agencies in the framework of JPI Oceans and other institutions
Microplastic-associated POPs:
Effects and Food Web Transfer
Andy Booth1, Iurgi Salaverria2, Paula Sobral3, Laura Airoldi4, Giulio Zanaroli4, Richard Sempere5, Susanne Kühn6, Kerstin Magnusson7, Thomas Doyle8, Liam Morrison8, Dorte Herzke9, Amaia Orbea10, Tamara Galloway11
Aim: To assess ingestion, accumulation and effects ofPOPs, metals and additive chemicals (ACs) associatedwith MPs to individual species, and follow transferbetween trophic levels.
Objectives
• Quantify uptake and accumulation of POPs, metalsand ACs associated with MPs.
• Study the effects of POPs, metals and ACsassociated with MPs.
• Determine if POPs, metals and ACs associated withMPs undergo trophic transfer.
• Investigate biotransformation of POPs associatedwith MPs by marine microbial communities.
1 SINTEF Ocean, Norway; 2 Norwegian University of Science and Technology, Norway; 3 FCT-NOVA, Portugal; 4 Alma Mater Studiorum - University ofBologna, Italy; 5 Aix-Marseille Université, Mediterranean Institute of Oceanography, France; 6 Wageningen Marine Research, Netherlands; 7 IVLSwedish Environmental Research Institute, Sweden; 8 National University of Ireland Galway, Ireland; 9 Norwegian Institute for Air Research, Norway;10 Universidad del País Vasco/Euskal Herriko Unibertsitatea, Spain; 11 University of Exeter, United Kingdom
https://www.sintef.no/projectweb/plastox/
Follow PLASTOXon Facebook x
Method developmentAim and Objectives
PLASTOX
WP4
Establish EquilibriumDissolve POP Add MPs
Exposure PreparationAccumulation Determined
Derive LC50/EC50
Derive LC50/EC50
Accumulation Determined
Accumulation & Toxicity AssessmentWill use a range of common POP and metal contaminants, selected MPs and test species.
In vivo and in vitro experiments with mussels
In vivo exposure of mussels to PS MPs previously incubated in 1 µM benzo(a)pyrene (BaP) leads to:• BaP accumulation in tissues
depends on MP size and exposure time
• Genotoxic damage in mussel haemocytes (Comet assay)
• No clear effects on physiological responses
In in vitro assays (MTT assay):• Co-exposure to nanoplastics and B(a)P or exposure to
microplastics (0.5 and 4.5 µm) with adsorbed B(a)P did not decreased cellular viability
[BaP] ng/g dw 7 days 26 days
0.5 µm MP + BaP 60.35 ± 8.98 292.05 ± 35.05
4.5 µm MP + BaP 27.15 ± 13.94 129.9 ± 47.80
Colonization of PCB-contaminated MPs by the bacterial community & biotransformation of MPs-adsorbed PCBs
• 5 types of MPs (PE, PET, PS, PP, PVC)• 2 sets of 20 mL microcosms: slurry
(sediment + water) and seawater from Piallassa Baiona (IT)
• Each consists of 2 sub-sets: o Pristine MPso PCB-contaminated MPs (Aroclor
1254, 30 mg/kg MP)• All microcosms inoculated with a PCB-
dechlorinating marine culture
3.5
3.8
4.1
4.4
4.7
5.0
5.3
5.6
5.9
0 5 10 15 20 25 30
Cl/
bip
he
nyl
Incubation Time (weeks)
PE PET PS PP
PVC SEDIMENT STERILE PE STERILE PET
STERILE PS STERILE PP STERILE PVC
Reductive dechlorination of sorbed PCBs
• PCB dechlorination faster on MPs than on sediment• Dechlorination of sorbed PBCs: PP < PE < PET < PVC < PS
1.E+00
1.E+01
1.E+02
1.E+03
1.E+04
1.E+05
1.E+06
1.E+07
1.E+08
1.E+09
1
10
100
1000
0 5 10 15 20 25 30
cells/g (P
VC
)
cells
/g (
PE,
PET
, PS,
PP
)
Incubation Time (weeks)
PE PET PS PP PVC
• No significant biofilm growth, except on PVC pellets (higher additive conc?)
Biofilm growth (crystal violet)
SMP = 0.5 µm MP; LMP = 4.5 µm MP; SMPB = 0.5 µm MP+BaP; LMPB = 4.5 µm MPs+BaP
Co-exposure to nanoplastics and BaP Exposure to microplastics with adsorbed BaP
Significant
differences (p<0.05)
with respect to
controls according
to the Kruskall-
Wallis analysis
followed by Dunn’s
post-hoc test with
Bonferroni’scorrection
In vivo studies, Joseph Hatfield
In vitro studies, Laura Sandoval
0.5 µm 4.5 µm0.05 µm MP + 1 µM BaP
Cont 102 104 105 106 107103 Cont 102 104 105 106 107103
particles/mL particles/mL
BaP 1 µM