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Platypus Technologies, LLC5520 Nobel Drive, Suite 100
Madison, WI 53711Toll Free: (866) 296-4455Phone: (608) 237-1270
Fax: (608) 237-1271
Cell Migration Assay Kit*
96-well, 2-D Assay for InvestigatingCell Migration of Adherent Cell Lines
* Patent Pending
Page 2
Platypus Technologies
Oris™ Cell Migration Assay
Inserts in 96-well Plate
Insert Removal Tool
Opaque MaskAssay Contains:
Easy-to-Read ResultsEasy-to-Read Results
Opaque MaskInserts in 96-well Plate
Insert Removal ToolPermits Easy-to-Read
Results
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Platypus Technologies
Oris™ Cell Migration Assay Procedure
Four Simple Steps
1. Apply the Mask
2. Add Cells & Incubate
3. Remove Inserts & Incubate
4. Observe Migrated Cells
Add Cells to the Well
Incubate to Allow Cells to Seed Into Outer
Annular Region of Well
Remove Insert
Incubate to Allow Cells to Migrate Into
Central Analytic Region of Well
Seeded Cells that HAVE NOT Migrated into the
Central Analytic Region are Blocked from View
Observe Migrated Cells
Using Microscope or Plate Reader
Apply the Mask
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Platypus Technologies
HT 1080 Cell Migration/Proliferation
Pre-Migration (T= 0)
Results of Oris™ Cell Migration Assay
Post-Migration (T= 21 hrs)
Kinetics of HT1080 Cell Migration
0
500
1000
1500
2000
2500
3000
0 5 10 15 20 25 30
Time (hrs)
# C
ells
in
Mig
rati
on
Zo
ne
n = 9 wells / time point
HT 1080 Cell Migration
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Platypus Technologies
• High Reproducibility
• Quantitative Kinetic and Endpoint Measurements
• 2 Dimensional Closure Assay
• Easy to Use / Less Hands-On-Time
• No Specialized Equipment
• Permits Limited Morphological Examination During
Migration
• Amenable to High-throughput Analysis
• May use Multiple Probes Simultaneously
Advantages of the Oris™ Cell Migration Assay
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Platypus Technologies
Contact information for the Oris™ Cell Migration Assay:
Platypus Technologies5520 Nobel Drive, #100
Madison, WI 53711
Phone: 608-237-1270, x 303Email: [email protected]
Web: www.platypustech.com
