GCLM Polyclonal antibodyCatalog Number:14241-1-AP 50 Publications
For Research Use Only
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Basic Information Catalog Number:14241-1-APSize:300 μg/mlSource:RabbitIsotype:IgGPurif ication Method:Antigen aff inity purif icationImmunogen Catalog Number:AG5497
GenBank Accession Number:BC041809GeneID (NCBI):2730Full Name:glutamate-cysteine ligase, modif ier subunitCalculated MW:31 kDaObserved MW:31 kDa
Recommended Dilutions:WB 1:1000-1:4000 IP 0.5-4.0 ug for IP and 1:500-1:2000 for WBIHC 1:100-1:400 IF 1:20-1:200
Applications Tested Applications:FC, IF, IHC, IP, WB, ELISACited Applications:IF, IHC, WBSpecies Specif icity:human, mouse, ratCited Species:human, mouse, ratNote-IHC: suggested an gen retrieval withTE buffer pH 9.0; (*) Alterna vely, an genretrieval may be performed with citratebuffer pH 6.0
Positive Controls:WB : HeLa cells; MCF-7 cells, L02 cells, A431 cells,mouse liver tissue, rat liver tissueIP : A431 cells;IHC : human liver cancer tissue; human skeletalmuscle tissueIF : HepG2 cells;
Background Information GCLM(Glutamate--cysteine ligase regulatory subunit) is also named as GLCLR and belongs to the aldo/keto reductase family. It catalyzes the f irst step ofGSH synthesis and is involved in gamma-glutamyl cycle. GCLM is implicated in some forms of hemolytic anemia.
Notable Publications Author Pubmed ID Journal Application
Jiawen Zheng 31569771 Mar Drugs WB
Xiaoying Tang 31548295 Mol Cancer Ther
Yijun Tian 27601007 J Hematol Oncol WB
Storage Storage:Store at -20ºC. Stable for one year af ter shipment.Storage Buffer:PBS with 0.02% sodium azide and 50% glycerol pH 7.3.Aliquoting is unnecessary for -20ºC storage
Selected Validation Data
Various lysates were subjected to SDS PAGEfollowed by western blot with 14241-1-AP (GCLMantibody) at dilution of 1:1000 incubated at roomtemperature for 1.5 hours.
Immunofluorescent analysis of HepG2 cells,using GCLM antibody 14241-1-AP at 1:50 dilutionand Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (f luorescent DNA dye).
IP Result of anti-GCLM (IP:14241-1-AP, 3ug;Detection:14241-1-AP 1:1000) with A431 cellslysate 3040ug.
Immunohistochemical analysis of paraff in-embedded human liver cancer tissue slide using14241-1-AP (GCLM Antibody) at dilution of 1:200(under 10x lens).
Immunohistochemical analysis of paraff in-embedded human liver cancer tissue slide using14241-1-AP (GCLM Antibody) at dilution of 1:200(under 40x lens).
1X10̂ 6 HepG2 cells were stained with 0.2ugGCLM antibody (14241-1-AP, red) and controlantibody (blue). Fixed with 90% MeOH blockedwith 3% BSA (30 min). Alexa Fluor 488-conjugatedAff iniPure Goat Anti-Rabbit IgG(H+L) with dilution1:1500.