Pooria GillPhD Of Nanobiotechnology

poor iagi l l@yahoo.com

STM Applications in Biomedicine

In The Name of Allah

Nanoscopy of Nanostructured Biomolecules

Structural AnalysesInteractiomicsPartial SequencingImmobilization CharacterizationPeptide CharacteristicsMicrobial CharacteristicsViral Characteristics…

Single strand of calf thymus DNA deposited along a surface step of HOPG. (50 x 50 nm, constant current mode, current 0.1 nA, bias voltage 500 mV.)

Methods In Molecular Biology, Vol 22. Microscopy, Opt/cat Spectroscopy, and Macmscop/c Technrqoes Edlted by: C Jones, I3 Mulloy, and A H. Thomas Copynght 01994 Humana Press Inc., Totowa, NJ.

STM of λ-DNA (GeneRuler DNA) on HOPG

P. Gill, B. Ranjbar, R. Saber. IET Nanobiotechnol., 2011, Vol. 5, Iss. 1, pp. 8–13.

3D image of a single antibody (IgG) molecule after the filtering and coloring process, which shows orientation of this molecule after physical adsorption on the

rigid surface from the hinge region imaged by NAMA-STM

R. Saber, S. Sarkar, P. Gill, B. Nazari, F. Faridani. Scientia Iranica F (2011) 18 (6), 1643–1646.

3D image of a single antibody (IgM) molecule, imaged by NAMA-STM. (b) Standard configuration of human immunoglobulin M with pentameric domains

R. Saber, S. Sarkar, P. Gill, B. Nazari, F. Faridani. Scientia Iranica F (2011) 18 (6), 1643–1646.

STM images of antiferritin antibodies passively adsorbed to a microwell surface

STM images of biotinylated antiferritin antibodies immobilized to a

streptavidin coated microwell surface

STM comparison of passive antibody adsorption and biotinylated antibody linkage to streptavidin on microtiter wells

Davies et al., Journal of Immunological Methods, 167 (1994) 263-269.

Individual Peptide Structures Visible by STM

Reconstructed surface topography of coated T4 polybead capsomeres; (a) the TEM and (b) STM representations. The slight variation in the representation may be due to the overlying carbon

film, which is observed by STM but not by TEM. Height range is 2.3 nm. (Reprinted with permission from Stemmer et al., 1989.)

M. FIRTEL and T. J. BEVERIDGE. Scanning Probe Microscopy in Microbiology. Micron, Vol. 26, No. 4, pp. 347-362, 1995.

Reconstructed surface topography of coated T4 polybead capsomeres; (a) the TEM and (b) STM representations. The slight variation in the representation may be due to the overlying carbon

film, which is observed by STM but not by TEM. Height range is 2.3 nm. (Reprinted with permission from Stemmer et al., 1989.)

M. FIRTEL and T. J. BEVERIDGE. Scanning Probe Microscopy in Microbiology. Micron, Vol. 26, No. 4, pp. 347-362, 1995.

STM image of coated (a) sheath and (b) hoops from M. hungatei. Bars: x, y = 100 nm; z = 8 nm.

M. FIRTEL and T. J. BEVERIDGE. Scanning Probe Microscopy in Microbiology. Micron, Vol. 26, No. 4, pp. 347-362, 1995.

Thanks for your Attentions