Post ASH 2014 - Actualités LMC
Actualités de première ligne LMC PC
• ENESTnd (6 ans)
• Dasision (5 ans)
• EPIC
• Spirit France (5 ans)
• Spirit 2 UK (5 ans)
ENESTnd: Mise à jour à 6 ans
Imatinib 400 mg QD (n = 283)
Nilotinib 300 mg BID (n = 282)RANDOMIZE
RANDOMIZE
Nilotinib 400 mg BID (n = 281)
Planned follow-up: 10 yearsa,b
N = 846
Adults with
newly
diagnosed
≤ 6 months)
Ph+ CML-CP1:1:1
Stratified by
Sokal risk
score
Larson RA, et al. Blood. 2014:[abstract 4541].
Design
ENESTnd: Mise à jour à 6 ans
Larson RA, et al. Blood. 2014:[abstract 4541].
a On core or extension treatment or in posttreatment follow-up. b Per the 2009 European LeukemiaNet criteria (Baccarani M, et al. J Clin Oncol. 2009;27:6041-6051) or investigator assessment. c Included abnormal test procedure results (n = 0 [nilotinib 300 mg BID], 1 [nilotinib 400 mg BID], and 1 [imatinib]), condition no longer required study drug (n = 1, 0, and 0, respectively), lost to follow-up (n = 4, 2, and 3, respectively), administrative problems (n = 7, 6, and 7, respectively), treatment duration completed per protocol (n = 3, 1, and 2, respectively), and protocol deviation (n = 14, 11, and 6, respectively).
Patient Status, n (%)Nilotinib
300 mg BID(n = 282)
Nilotinib 400 mg BID
(n = 281)
Imatinib 400 mg QD
(n = 283)
Still on studya 231 (81.9) 238 (84.7) 224 (79.2)
Still on core treatment 151 (53.5) 155 (55.2) 127 (44.9)
Discontinued core treatment and entered extension study 24 (8.5) 3 (1.1) 45 (15.9)
Discontinued core treatment without entering extension study 107 (37.9) 123 (43.8) 111 (39.2)
Adverse event/laboratory abnormalities 39 (13.8) 64 (22.8) 39 (13.8)
Withdrawal of consent 19 (6.7) 22 (7.8) 22 (7.8)
Suboptimal response/treatment failureb 11 (3.9) 10 (3.6) 19 (6.7)
Death 7 (2.5) 2 (0.7) 2 (0.7)
Disease progression 2 (0.7) 4 (1.4) 10 (3.5)
Other reasonc 29 (10.3) 21 (7.5) 19 (6.7)
Patients’ disposition
ENESTnd: Mise à jour à 6 ans
Larson RA, et al. Blood. 2014:[abstract 4541].
0 6 12 18 24 30 36 42 48 54 60
0
20
40
60
80
100
Months Since Randomization
Cu
mu
lati
ve
In
cid
en
ce
of
MR4.5
, %
Nilotinib 300 mg BID (n = 282)Nilotinib 400 mg BID (n = 281)
Imatinib 400 mg QD (n = 283)
10
30
50
70
90
66 72 78
11%; P < .0001a
7%; P < .0001a
1%
∆ 6% to 10%
By 1 Year
56%; P < .0001a
55%; P < .0001a
33%
∆ 22% to 23%
By 6 Years
31%
By 5 Years
54%; P < .0001a
52%; P < .0001a
∆ 21% to 23%
Cumulative incidence of MR4.5
Nilotinib
300 mg BID
(n = 282)
Nilotinib
400 mg BID
(n = 281)
Imatinib
400 mg QD
(n = 283)
Progression to AP/BC on core treatment, n 2 3 12
KM-estimated 6-year freedom from
progression to AP/BC (95% CI), % 99.3
(98.2-100)
98.7
(97.2-100)
95.2
(92.6-97.9)
Hazard ratio vs imatinib (95% CI)0.1599
(0.0358-0.7143)
0.2457
(0.0693-0.8713)–
Nominal P value vs imatinib .0059 .0185 –
Progression to AP/BC on study, n 11 6 21
KM-estimated 6-year freedom from
progression to AP/BC (95% CI), %
95.8
(93.3-98.2)
97.8
(96.0-99.5)
92.2
(89.1-95.4)
Hazard ratio vs imatinib (95% CI)0.5110
(0.2464-1.0600)
0.2773
(0.1119-0.6870)–
Nominal P value vs imatinib .0661 .0030 –
ENESTnd: Mise à jour à 6 ans
Larson RA, et al. Blood. 2014:[abstract 4541].
Freedom from progression to AP/BC at 6 years
ENESTnd: Mise à jour à 6 ans
Larson RA, et al. Blood. 2014:[abstract 4541].
Overall survival and deathsNilotinib
300 mg BID
(n = 282)
Nilotinib
400 mg BID
(n = 281)
Imatinib
400 mg QD
(n = 283)
Total deaths on study, na 21 11 23
KM-estimated 6-year OS on study
(95% CI), %91.6 (88.0-95.1) 95.8 (93.4-98.2) 91.4 (88.0-94.7)
Hazard ratio vs imatinib (95% CI) 0.8934
(0.4944-1.6143)
0.4632
(0.2258-0.9503)–
Nominal P value vs imatinib .7085 .0314 –
Deaths due to advanced CML, n 6 4 16
KM-estimated 6-year freedom from
death due to advanced CML (95% CI),
%
97.7 (96.0-99.5) 98.5 (97.1-100) 93.9 (91.0-96.8)
Hazard ratio vs imatinib (95% CI)0.3694
(0.1445-0.9440)
0.2433
(0.0813-0.7279)–
Nominal P value vs imatinib .0302 .0061 –
Dasision: Mise à jour à 5 ans
Cortes J, et al. Blood. 2014:[abstract 152].
Design
Database lock of 24-Mar-2014
Primary end point: confirmed CCyR by 12 months
• 77% dasatinib vs. 66% imatinib (P=0.007)1
5-year
final resultsRandomized a
Imatinib 400 mg QD (n=260)Imatinib 400 mg QD (n=260)
Dasatinib 100 mg QD (n=259)Dasatinib 100 mg QD (n=259)
�Treatment-naïve
CML-CP patients
(N=519)
�108 centers
�26 countries
�Enrollment:
September 2007–
December 2008
�Treatment-naïve
CML-CP patients
(N=519)
�108 centers
�26 countries
�Enrollment:
September 2007–
December 2008
a Stratified by EURO (Hasford) risk score.
1. Kantarjian H et al. N Engl J Med 2010;362:2260
Dasision : Mise à jour à 5 ans
Cortes J, et al. Blood. 2014:[abstract 152].
Patients’ disposition
Treated Patients, n (%)
Dasatinib 100 mg QD
(n=258)
Imatinib 400 mg QD
(n=258)
On initial therapy at study end 158 (61) 162 (63)
Discontinued
Progression or treatment failure 28 (11) 36 (14)
AE related to study treatment a 42 (16) 17 (7)
AE unrelated to study treatment a 12 (5) 4 (2)
Poor/nonadherence 1 (<1) 7 (3)
Other 17 (7) b 31 (12) c
a As defined by investigator.b Includes withdrawal of consent and patient request (4 each), insufficient molecular response (3), pregnancy (2), and lost to follow-up, loss of CCyR, increased BCR-ABL,
and relocation to the US (1 each).c Includes patient request (10), no molecular response/loss of molecular response (4), withdrawal of consent and suboptimal response (3 each), lost to
follow-up, insufficient cytogenetic response, and investigator request (2 each), and pregnancy, recurrence of blasts in bone marrow, no CMR, no MMR,
and appearance of mutation (1 each).
Dasision: Mise à jour à 5 ans
Cortes J, et al. Blood. 2014:[abstract 152].
CI MR4.5
0 6 12 18 24 30 36 42 48 54 60
100
90
80
70
60
50
40
30
20
10
0
By 1 year
By 2 years
By 3 years
By 4 years
By 5 years
3%
8%
13%
23%
33%
5%
19%
24%
34%
42%
p=0.0251
Months Since Randomization
% W
ith
MR4.5
Dasatinib 100 mg QD
N
Imatinib 400 mg QD 260259
One imatinib patient and no dasatinib patients transformed between 4 and 5 yearsOne imatinib patient and no dasatinib patients transformed between 4 and 5 years
Dasatinib 100 mg QD
(n=259)
Imatinib 400 mg QD
(n=260)
BCR-ABL at 3 Months a≤10%
n=198
>10%
n=37
≤10%
n=154
>10%
n=85
Transformation to AP/BP b, n (%) 6 (3) 5 (14) 5 (3) 13 (15)
Pa
tie
nts
, n
Pa
tie
nts
, n
4.6%4.6%
7.3%7.3%
Dasatinib n=259Dasatinib n=259 Imatinib n=260Imatinib n=260
On studyOn study During follow-up beyond discontinuationDuring follow-up beyond discontinuationOn study During follow-up beyond discontinuation
a One dasatinib and one imatinib patient transformed but did not have 3-month molecular assessments.
b Including follow-up beyond discontinuation (intent to treat).
Dasision: Mise à jour à 5 ans
Cortes J, et al. Blood. 2014:[abstract 152].
Transformation to AP/BC
Dasatinib100 mg QD
(n=259)
Imatinib400 mg QD
(n=260)
Hazard ratio
(95% CI)
Total number of deaths, n 26 26 –
Estimated 5-year OS, % (95% CI)
91(87–94)
90(85–93)
1.01 (0.58–1.73)
Estimated 5-year PFS, % (95% CI)
85(80–89)
86(80–89)
1.06 (0.68–1.66)
Causes of death were cardiovascular disease (2 dasatinib, 1 imatinib); disease progression
(9 dasatinib, 17 imatinib); infection (11 dasatinib, 1 imatinib); other malignancy, septic shock and cardiac failure,
multi-organ failure, and whole body swelling (1 each dasatinib); stem cell transplantation complications and
unknown (2 each imatinib); severe chest pain, clinical deterioration and decrease in performance status, and fatal
bleeding (1 each imatinib)
On-study treatment and in follow-up after discontinuation of randomized treatment.
Dasision: Mise à jour à 5 ans
Cortes J, et al. Blood. 2014:[abstract 152].
Overall and Progression-free survival
EPIC
Lipton JH, et al. Blood. 2014:[abstract 519].
Design
EPIC
Lipton JH, et al. Blood. 2014:[abstract 519].
Molecular results at 3, 6, 9 and 12 months (Evaluable patients)
EPIC
Lipton JH, et al. Blood. 2014:[abstract 519].
Treatment-emergent vascular events = 14% emergent CV events in total= 14% emergent CV events in total
SPIRIT France
F, et al. Blood. 2014:[abstract 1793].
Survie à 5 ans
Survie globaleSurvie globale Survie sans progressionSurvie sans progression
SPIRIT 2 : Study Design
Chronic phase CML
within 3 months of diagnosis R
Arm AImatinib 400
Arm BDasatinib 100
Randomised, open labelPrimary endpoint: 5 year EFSSecondary: cytogenetic, PCR response, toxicity
n=814
N=407
N=407
EndpointsPrimary� 5 year event free survival (EFS)
– Assessed for all patients March 2018
Secondary� Rate of complete cytogenetic response (CCR)� Rate of Major Molecular Response
– (MMR, MR3, BCR-ABL1/ABL1 ratio<0.1%)
� Toxicity� Treatment failure rates (TFR) after 5 years� Rates of complete haematologic response (CHR)� Overall survival at 2 and 5 years
Patients Who Stopped Study Drug
Reason for stopping study drug (exc. death)Imatinib406 (%)
Dasatinib406 (%)
Total812 (%)
Consent withdrawn 7 (1.7) 9 (2.2) 16 (2.0)Disease progression - accelerated phase 1 (0.2) 2 (0.5) 3 (0.4)Disease progression - blast crisis 7 (1.7) 4 (1.0) 11 (1.4)Failure to achieve CCR after 24 months 4 (1.0) 1 (0.2) 5 (0.6)Failure to achieve MCR after 12 months 23 (5.7) 3 (0.7) 26 (3.2)Intolerance - non haem tox 53 (13.1) 80 (19.7) 133 (16.4)Intolerance - haem/lab tox 10 (2.5) 10 (2.5) 20 (2.5)Loss of CHR 5 (1.2) 0 5 (0.6)Loss of MCR 5 (1.2) 2 (0.5) 7 (0.9)Other reason 4 (1.0) 11 (2.7) 29 (3.6)Reason unknown - Lost to follow up 2 (0.5) 0 2 (0.2)
‘Inadequate response’ (cytogenetic, haematological, molecular, mutation detected) 42 (10.3) 3 (0.7) 45 (5.5)
Total 163 (40.1) 125 (30.8) 288 (35.5)
Cause of Death
CML related 6/18 (33%)
Non – CML related 9/18 (50%)
Total Deaths38/812 (4.7%)
Imatinib18/406 (2.2%)
Dasatinib20/406 (2.5%)
CML related 5/20 (25%)
Non – CML related 10/20 (50%)
Unknown3/18 (17%)
Unknown5/20 (25%)
Other cancer4/9 (44%)
Non cancer5/9 (56%)
BronchogenicEndometrialRectalGastric
• Left ventricular failure• Bronchopneumonia secondary to
emphysema• Myocardial infarction • Ruptured aortic aneurysm• Bronchopneumonia
Other cancer3/10 (30%)
Non cancer7/10 (70%)
• Colon ca• Lung ca• Metastatic breast ca
• Ischaemic heart disease, COPD, CCF
• Chest Infection, CCF, renal failure, diabetes
• Bowel perforation• Cardiac failure and
bronchopneumonia• Liver disease• COPD• Chronic cardiac failure
11
Comparative AEsFluid retention
Oedema
Pleural effusion
Myalgia
Nausea
Vomiting
Diarrhoea
Fatigue
Headache
Rash
Pulmonary (arterial) hypertension
Dyspnoea (exertional) with no pleural effusion
Favours dasatinib Favours imatinib
Difference
95% CIs
November 2014
Cytogenetics at 12 Months
Imatinib (%) Dasatinib (%) Difference (%) p value
Major cytogenetic response (MCR)
209/406(51.5)
228/406(56.2)
(4.7) 0.181*
Complete cytogenetic response (CCR)
169/406 (41.6)217/406(53.4)
(11.8) <0.001*
Missing analyses181/406 (44.6)
166/406(40.9)
*caution required, missing analyses included in denominator
12 Month PCR
Dasatinibn=406
Achieved MR4.5 Response
24/406(5.9%)
Achieved MR4.5Response
54/406(13.3%)
On treatment320/406 (78.8%)
Off treatment 80/406 (19.7%)
Unknown6/406 (1.5%)
On treatment344/406 (84.7%)
Off treatment59/406 (14.5%)
Unknown3/406 (0.7%)
Achieved MR3Response
175/406(43.1%)
Achieved MR3Response
237/406(58.4%)
Total Cohortn=812
∆ = 7.4% P=0.001
∆ = 15.3% p<0.001
November 2014
Imatinibn=406
Partial PCR Responses
Imatinib Dasatinib Totals
3 month PCR samples available 317 100% 319 100% 636 100%
PCR > 10% (MR1) at 3 months 66 20.8% 20 6.3% 86 13.5%
PCR < 10% (MR1) at 3 months 251 79.2% 299 93.7% 550 86.5%
12 month PCR samples available 210 100% 267 100% 477 100%
PCR > 1% (MR2) at 12 months* 20 9.5% 10 3.7% 30 6.3%
PCR < 1% (MR2) at 12 months* 190 90.5% 257 96.3% 447 93.7%
Total with 'less than ideal' progress 86/317 27.1% 30/319 9.4% 116/636 18.2%
PCR Data: All Patients, Both Arms
PC
R R
atio
(B
CR
-AB
L1/A
BL1
Rat
io)
IS
7,431 data points, IS
Months From Randomisation
11 Patients Who ‘Died From CML’
No follow up data 3 overt blast crisis• 10.6 month interval to death• 12.6 months• 14.2 months1 equivocal accelerated phase• 42.4 months
PC
R R
atio
(B
CR
-AB
L1/A
BL1
Rat
io)
IS
Months From Randomisation
Actualités de première ligne LMC PC
Prognostic Significance of Early Predictors
Comparing the Prognostic Significance of Early Predictors of Survival in Chronic Myeloid Leukemia (CML) Treated with Imatinib - an
Analysis of the Randomized CML-Study IV
�BackgroundEarly prediction of outcome using response-related predictive landmarks has become a major paradigm in the clinical management of chronic myeloid leukemia (CML)
�Aims• Compare the prognostic significance of early predictors of survival according
to sensitivity and specificity of 3 Mo and 6 Mo landmarks
• Apply landmarks in patients with disease progression (AP, BP, deaths) within the CML study IV
A156
Hanfstein B, et al. Comparing the Prognostic Significance of Early Predictors of Survival in Chronic Myeloid
Leukemia (CML) Treated with Imatinib - an Analysis of the Randomized CML-Study IV.
Comparing the Prognostic Significance of Early Predictors of Survival in Chronic Myeloid Leukemia (CML) Treated with Imatinib - an
Analysis of the Randomized CML-Study IV
� A total of 967 newly diagnosed patients were assigned to an imatinib-based treatment arm of CML-Study IV Median follow-up: 7.1 years
� The number of molecular assessments:
n= 789 (at 6 months)
n= 692 (at 3 months)
n= 301 (at 3 months and at diagnosis, without pretreatment)
� At 3 and 6 months, a BCR-ABL ratio was calculated using ABL as reference gene and standardized. At +3 mo a standardization was also made by GUS as a reference gene in order to ensure the linearity of the data
A156
Hanfstein B, et al. Comparing the Prognostic Significance of Early Predictors of Survival in Chronic Myeloid
Leukemia (CML) Treated with Imatinib - an Analysis of the Randomized CML-Study IV.
Applying early landmarks on patients with disease
progression in CML-study IV
� Patient sample
3-month and 6-month molecular assessment: n=513
� Events defined by disease progression (accelerated phase, blastic phase or death) later than 7.5 months:
• Total: n= 45
• Accelerated phase: n= 4
• Blastic phase: n= 11
• Death from any reason: n= 30
• 2nd TKI: n= 21
• SCT: n= 13
A156
How could patients at risk be identified early and more precisely?
Hanfstein B, et al. Comparing the Prognostic Significance of Early Predictors of Survival in Chronic Myeloid Leukemia (CML) Treated with Imatinib - an Analysis of the Randomized CML-Study IV.
Applying early landmarks on patients with disease
progression in CML-study IV
Conclusions� The 3-month 10% BCR-ABLIS landmark identifies patients at risk of
progression with a sensitivity of 41% and a specificity of 75%
� The 6-month 10% BCR-ABLIS landmark is less sensitive (18%) and more specific (94%)
� The most precise identification of patients at risk is allowed by a comparison of BCR-ABLIS at diagnosis and at 3 months
Hanfstein B, et al. Comparing the Prognostic Significance of Early Predictors of Survival in Chronic Myeloid
Leukemia (CML) Treated with Imatinib - an Analysis of the Randomized CML-Study IV.
A half-log reduction of BCR-ABL transcripts at 3 mo nths is associated with an 8-year PFS of 94% vs 75% (43%
sensitivity, 87% specificity)
Applying early landmarks on patients with disease
progression in CML-study IV
A156Progression-free survival (PFS) according to 0.5-lo g
reduction of BCR-ABL at 3 months
Hanfstein B, et al. Comparing the Prognostic Significance of Early Predictors of Survival in Chronic Myeloid
Leukemia (CML) Treated with Imatinib - an Analysis of the Randomized CML-Study IV.
Essais d’arrêt de traitement
• Euroski (3 ans)
• Stop TKI2G (7 ans)
Relapse defined as BCR-ABL > 0.1% (loss of MMR) on the IS at one time point
Mahon FX, et al. Blood 2014:[abstract 151].
Molecular Relapse-free survival
At 6 months : 63 % (95% CI : 55% - 69%)At 12 months: 56 % (95% CI : 49 % - 63 %)At 18 months : 55 % (95% CI : 47 % - 61 %)
Relapses within 6 months , n=77
FX, et al. Blood. 2014:[abstract #151]
Molecular Relapse-free survival200 interim patients by TKI duration
Pts treated > 8 y N = 86, loss MMR = 29Pts treated < 8 y N = 114, loss MMR = 60
Overall p=0.007
At 18 months : > 8 y : 65 % (95% CI : 53 % - 74%)< 8 y : 47 % (95% CI : 38 % - 56%)
FX, et al. Blood. 2014:[abstract #151]
Molecular Relapse-free survival200 interim patients by MR4 duration
> 5 y : N = 92, loss MMR = 32< 5 y : N = 108, loss MMR = 57
Overall p=0.0122
At 18 months : > 5 y : 65 % (95% CI : 54 % - 74%)< 5 y: 46 % (95% CI : 38 % - 56%)
FX, et al. Blood. 2014:[abstract #151]
Study design and objectives
Rea D. et al. Blood 2014; Abstract #811.
- Adult CP-CML patients (n=100 planned)
- TKI therapy for at least 3 years
- 2G-TKI frontline or after intolerance/resistance to imatinib
- Undetectable BCR-ABL* (CMR4.5) for at least 2 years
*Major BCR. Molecular monitoring performed in local laboratories filling international standardization requirements. At least20000x2 copies of ABL control gene until 2012 then at least 32000 copies (Cross et al. Leukemia 2012; 26: 2172-2175.)
→ Primary objective: treatment-free survival in MMR
M12 M60STOP
RQ-PCRmonthly
RQ-PCRq3-6 months
RQ-PCRq2-3 months
M24 M36 M48
Year 1 Year 2 Year 3-5
Treatment-free survival in MMR
24 patients lost MMR after a median time of 3.7 months (1.5-37.6)
Rea D. et al. Blood 2014; Abstract 811.
0
20
40
60
80
100
0 6 12 18 24 30 36 42 48 54 60
At 12 months: 59.6% (95% CI: 46.2-72.9)
At 24 months: 57.4% (95% CI: 43.9-70.9)
Months after 2G TKI discontinuation
% T
rea
tme
nt-
fre
e s
urv
iva
l in
MM
R
KM analysis
Treatment-free survival in MMR according to
baseline factors� No association between treatment-free survival without MMR loss and the following factors was found:
age, gender, Sokal risk group, exposure to IFN, treatment/CMR duration and type of 2G-TKI.
� Only prior history of suboptimal response or resistance to imatinib was found to have a significant impact:
Rea et al. Blood (ASH) 2014; Abstract 811.
KM analysis
0
20
40
60
80
100
0 6 12 18 24 30 36 42 48 54 60
Months after 2G TKI discontinuation
% T
rea
tme
nt-
fre
e s
urv
iva
l in
MM
R
P=0.048
First line/imatinib intolerant: 65.6% (95% CI: 50,2-79.7).
Suboptimal/resistant to imatinib: 41.6% (95% CI: 13.7-65.5).
At 12 months:
Rea D. et al. Blood 2014; Abstract #811.
Treatment-free survival in MMR according to BCR-ABL
levels at 3 months
Landmark analysis among 40 patients who remained treatment-free in MMR at 3 months.
Rea et al. Blood (ASH) 2014; Abstract 811.
CMR at 3 months n=23
No CMR at 3 months n=17
MR4.5 at 3 months n=30
No MR4.5 at 3 months n=10
KM analysis
0
20
40
60
80
100
0 6 12 18 24 30 36 42 48 54 60
Months after 2G TKI discontinuation
% T
reat
men
t-fr
ee s
urvi
val i
n M
MR
P=0.019
At 12 months: 91.3% (95%CI: 79.7-100)
At 12 months: 58.8% (95%CI: 35.4-82.2)
0
20
40
60
80
100
0 6 12 18 24 30 36 42 48 54 60% T
reat
men
t-fr
ee s
urvi
val i
n M
MR
Months after 2G TKI discontinuation
P=0.000007
At 12 months: 93.3% (95%CI: 84.4-100)
At 12 months: 30% (95%CI: 15.9-58.4)
Rea D. et al. Blood 2014; Abstract #811.
Early Disease Relapse after Tyrosine Kinase Inhibitor Treatment Discontinuation in CML Is Related Both
to Low Number and Impaired Function of NK-Cells
� Aim of the study:
To identify predictive biomarkers for relapse/non relapse after TKI discontinuation
� CML-CP Nordic patients included in the EURO-SKI study (n=119)
� Basic lymphocyte immunophenotyping (number of NK-, T-, and B-cells) performed at baseline and 1, 6 and 12 months after TKI discontinuation
� Functional analyses (cytotoxicity of NK-cells, Th1 cytokines) were performed in some patients
Patients with at least 6 months of follow-up after imatinib discontinuation (n=58)
Baseline variables Patients without loss of MMR(n=36)
Patients with loss of MMR(n=22)
p
NK-cells count (109cells/L) 0.26 0.15 0.01
NK-cells proportion (%) 18 11 0.005
NK-cells cytotoxic phenotype 1 19.2 13 0.02
Ilander MM, et al. Early Disease Relapse after Tyrosine Kinase Inhibitor Treatment Discontinuation in CML Is Related Both to Low Number and
Impaired Function of NK-Cells.
A812
(1) Based on levels of expression of CD57, CD16, CD 62L and IFN-ϒ/TNF-α secretion.
Early relapsing patients have lower proportion and number of Nkcells compared to non-relapsing or late-relapsing patients1) Based on levels of expression of CD57, CD16, CD62 L and IFN-ϒ/TNF-α secretion.
MM, et al. Early Disease Relapse after Tyrosine Kinase Inhibitor Treatment Discontinuation in CML Is Related Both to Low Number and Impaired Function of NK-Cells.
Early Disease Relapse after Tyrosine Kinase Inhibitor
Treatment Discontinuation in CML Is Related Both
to Low Number and Impaired Function of NK-Cells
Actualités de ≥ 2° ligne LMC PC
• PACE (3 ans)• BMS 034 (7 ans)
Essai PACE
Cortes J, et al. Blood. 2014:[abstract 3135].
Patients’ disposition
Essai PACE
Cortes J, et al. Blood. 2014:[abstract 3135].
Survival for CP CML
Median follow-up: 38 months
Essai PACE
Cortes J, et al. Blood. 2014:[abstract 3135].
Survival
Median follow-up: 34 months
Essai PACE
Cortes J, et al. Blood. 2014:[abstract 3135].
Adverse Events = 49% emergent CV events in total= 49% emergent CV events in total
CA180-034 Study Design
� After 2 years, protocol allowed switching from BID to QD dosing
� Primary endpoint: To compare the MCyR rates of dasat inib when administered QD vs BID after a minimum follow-up of 6 months
N=662 treated
100 mg QD (n=167)
140 mg QD (n=167)
50 mg BID ( n=168)
70 mg BID ( n=168)
CP-CML pts ≥18 years with• Resistance• Suboptimal
response• Intolerance
to imatinib
N=670 randomized a
7-year
final results
Enrollment period: July 13, 2005 – March 13, 2006.
Patients were stratified by imatinib resistance vs. imatinib intolerance.
Duration of TherapyPatients in the 100 mg QD arm:� Remained on assigned therapy longer compared to oth er
dose groups� Maintained their assigned dose in a greater proport ion of
patients (38%) After 2 years:� The majority of patients on BID dosing arms were sw itched to
QD dosing� 19% of patients in the 140 mg QD arm treated after amendment switched to 100 mg QD
dosing by last recorded dose
100 mq QD(n=165)
140 mg QD(n=163)
50 mg BID(n=167)
70 mg BID(n=167)
Total(n=662)
Median duration, m 37 27 28 29 30
≥7 years dasatinib, % 22 15 19 21 19
Data are reported according to dose assigned at study entry.
Number of Treated Patients (%)
Dasatinib Dose100 mg QD
(n=166)140 mg QD
(n=163)50 mg BID a
(n=166)70 mg BID
(n=167)
Protocol -definedprogression b 35 (21) 42 (26) 29 (17) 27 (16)
Study drug toxicity 39 (24) 45 (28) 45 (27) 51 (31)
AE unrelated to study drug 10 (6) 4 (3) 10 (6) 8 (5)
Investigator request 12 (7) 6 (4) 7 (4) 5 (3)
Patient request 14 (9) 19 (12) 18 (11) 16 (10)
Other 16 (10) 15 (9) 19 (11) 22 (13)
Reason for DiscontinuationPrior to Study Closure
� The majority of patients classified as “other” cont inued to receive dasatinib
Reason for discontinuation was not reported in one patient in the 50 mg BID arm.Protocol-defined progression included increasing WBC count, loss of CHR or MCyR, ≥30% increase in Ph+ metaphases, or transformation
to AP/BP.
Safety
Number of Patients (%)
Dasatinib Dose100 mg QD
(n=165)
Other Dose Groups(n=497)
Total(n=662)
Drug -related AE 150 (91) 471 (95) 621 (94)
Grade ≥3 drug -related AE 75 (46) 281 (57) 356 (54)
Serious drug -related AE 43 (26) 173 (35) 216 (33)
Drug -related AE leading to discontinuation 34 (21) 130 (26) 164 (25)
Death 51 (31) 133 (27) 184 (28)
Study drug toxicity a 1 (1) 2 (<1) 3 (<1)
Disease progression 28 (17) 54 (11) 82 (12)
Safety and tolerability were assessed using the National Cancer Institute Common Terminology Criteria for Adverse Events v3.0.
Deaths due to study drug toxicity include 1 due to sepsis; 1 due to pulmonary edema, congestive heart failure, neck pain, and pleural effusion; due to necrosis of the colon.
Cumulative Incidence Rate of All-Cause Nonhematologic AEs of Special Interest
� For 100 mg QD, most nonhematologic AEs (all grades) first occurred within 24 months of treatment
0 5 10 15 20 25 30 35 40 45 50
Rash
Myalgias/arthralgias
Fatigue
Nausea/vomiting
Diarrhea
Pleural effusion
Hemorrhage
Patients, %
100 mg QD (Any grade)
Other Dose Groups (Any grade)
100 mg QD (Grade ≥3)
Other Dose Groups (Grade ≥3)
Efficacy Results:Cumulative Incidence of MMR
� Over 7 years of follow -up, cumulative incidence of MMR was similar across dose groups
100 mg QD(n=167)
140 mg QD(n=167)
50 mg BID(n=168)
70 mg BID(n=168)
Assessed treated patients, n (%) 160 (96) 153 (92) 160 (95) 151 (90)
MMR in assessed treated patients, n (%)
73 (46) 68 (44) 70 (44) 69 (46)
OS is Similar Across Dose Groups
162154158157
154150153150
147147147142
143141140136
141132129129
137126124123
130120120113
120118114106
112114105104
1061069995
991019692
94979289
89948983
73767974
100 mg QD140 mg QD50 mg BID70 mg BID
167167168168
100
90
80
70
60
50
40
30
20
10
0
% A
live
100 mg QD140 mg QD50 mg BID70 mg BID
Months
65%
73%
68%70%
0 6 12 18 24 30 36 42 48 54 60 66 72 78 84
Imatinib-resistant Patients Imatinib-intolerant Pati ents Overall
OS, % (95% CI) 63 (53–71) 70 (52–82) 65 (56–72)
PFS, % (95% CI) 39 (29–49) 51 (32–67) 42 (33–51)
OS by 3-Month BCR -ABL Level: 100 mg QD
8658
8658
8657
8455
8255
8253
8051
7950
7849
7848
7748
7548
7547
7446
7244
7043
6741
6540
6239
6039
5938
5936
5635
5544
5333
5232
5130
4927
4521
Patients at Risk
%>10%
Unstratified Log Rank P-Value = 0.0374
≤10%>10%
72%
56%
BCR-ABL ≤10% at 3 months (60%) BCR-ABL >10% at 3 months (40%)
OS, % (95% CI) 72 (60–81) 56 (42–68)
PFS, % (95% CI) 56 (43–67) 21 (10–34)
100
90
80
70
60
50
40
30
20
10
0
3 9 15 21 27 33 39 45 51 57 63 69 75 810 6 12 18 24 30 36 42 48 54 60 66 72 78 84
% A
live
Months
Other results….
The Experience of the International Registry for CML in Children and Adolescents
(I-CML-Ped Study): Pronostic Consideration
A521 Results-1 N=278
Age, Median (yrs)< 4 yrs (%)
12.4 (9-17.5)6
Sex - Female (%) 43
Phase at diagnosis (%)ChronicAcceleratedBlastique
9281
Sokal risk score (%)LowIntermediateHigh
183151
Splenomegaly (%)Median spleen size (below costal margin) (cm)
7611 (1-25)
Median leucocyte count (x109/L) 235 (5-1038)
Additional chromosomal abnormalities in Ph+ cells (% pts.) 6
Median follow-up (mo) 39 (0.5 – 161)
F, et al. The Experience of the International Registry for Chronic Myeloid Leukemia (CML) in Children and Adolescents (I-CML-Ped Study):
Pronostic Consideration.
The Experience of the International Registry for CML in Children and Adolescents
(I-CML-Ped Study): Pronostic Consideration
1) 169 pts. with available evaluation, CCyR in 124. 2) In univariate analysis. 3) Multivariate analysis.
A521
N=278
Estimated overall survival at 60 months (%) 95% (95%CI 89-97)
Cumulative incidence of CCyR 1 73%
CCyR Predictive factors 2 Eutos scoreSpleen sizeHematocrit levelLymphocyte countImmature cells in peripheral blood
CCyR Predictive factors 3 Sokal and medullary blast count
Children and adolescents with CML presented with clinical and biological differences compared to adult patients
F, et al. The Experience of the International Registry for Chronic Myeloid Leukemia (CML) in Children and Adolescents (I-CML-Ped Study):
Pronostic Consideration.
CP-CML pediatric cohort – Imatinib upfront
N=102
Age, Median (yrs) at imatinib initiation 12 (1-18)
Prepubertal (< 10 yrs), n (%) 27 (26.4)
Pubertal (age:10-14 yrs), n (%) 46 (45)
Postpubertal (age > 14 yrs), n (%) 29 (28.4)
Sex - Female (%) 47
Median duration of imatinib exposure (mo) 9 (0-98)
Change of SDS height on therapy
All cohort Decrease of 0.48 SDS per year during the first 3 years of therapy
Pubertal (age:10-14 yrs), n (%) 0.75 SDS per year during the first 3 years of therapy
Impairment of Longitudinal Growth By Tyrosine Kinase Inhibitor (TKI) Treatment – Data from a Large Pediatric Cohort with Chronic Myeloid Leukemia (CML)
� Aim:
To determine the impact of sex, age, and pubertal stage on impaired growth in a
pediatric cohort CP-CML treated with imatinib up-front
� Retrospective German study
JT, et al. Impairment of Longitudinal Growth By Tyrosine Kinase Inhibitor (TKI) Treatment – Data from a Large Pediatric
Cohort with Chronic Myeloid Leukemia (CML).
A522
SDS: Standard deviation scores
60
Impairment of Longitudinal Growth By Tyrosine Kinase Inhibitor (TKI) Treatment – Data from a Large Pediatric Cohort with Chronic Myeloid Leukemia (CML)
Changes in SDS body height analyzed at 3 months intervals during TKI therapy n=102 pts(age at diagnosis: 12 years, range: 1-18 years; 54 male, 48 female)
JT, et al. Impairment of Longitudinal Growth By Tyrosine Kinase Inhibitor (TKI) Treatment – Data from
a Large Pediatric Cohort with Chronic Myeloid Leukemia (CML).
Growth retardation is a significant adverse effect of IMA in children with CML affecting predominantly prepubertal children
KCL-22 CML Xenograft
Combination of ABL001 and Nilotinib prevents the emergence of resistance
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180 180
Nilotinib (75mg/kg) BIDNilotinib (75mg/kg) BID
ABL001 (30mg/kg) BIDABL001 (30mg/kg) BID
Nilotinib (75mg/kg) BID + ABL001 (30mg/kg) BIDNilotinib (75mg/kg) BID + ABL001 (30mg/kg) BID
Dosing stopped on day 77, all mice remain disease f ree >176 daysDosing stopped on day 77, all mice remain disease f ree >176 days
T315I detected
A337V detected
Each line represents individual animals
* *
*Wylie A, et al. Blood. 2014:[abstract 398].