Basics of Chromatography
• Separation of Analytes• Type of Equilibrium
M
S
CCK
Basics of Chromatography
• Ability to dissolve in mobile phase
• Interaction with stationary phase
• Time on X axis• Response on Y
• Chromatography Types
• Separation dependent on time on the column (along with other factors)
• Retention time of the analyte• Retention time of the Mobile Phase
Basics of Chromatography
• Average linear flow rate• Average linear flow of mobile phase
RtL
MtL
• Plate Height • Theoretical Measure
of Efficiency
LH
2
• Fig. 26-6• Determining standard deviation from a peak• Std dev = τ W = 4 τ
Basics of Chromatography
• Capacity Factor• Selectivity Factor
M
SA
VVK
k 'M
MR
tttk
'
'
'
A
B
kk
MAR
MBR
tttt
)()(
Basics of Chromatography
• The Van Deemter Equation
H = A + B/u + Cu
• Multiple Flow Paths• Tortuosity
• Components of the VanDeemter
• Van Deemter Plot
• How Plate Height is effected by particle size in solid packing
Basics of Chromatography
• Column Efficiency
HLN
2)(WtN R
2
21
)(54.5WtN R
Basics of Chromatography
• Resolution
WttR ARBR
S)()(
The Effect of Mobile Phase on Retention
More on Resolution