PRELAB: MITOSIS INTERNET LESSONhttp://teachers.olatheschools.com/apaepkeonw/

-INTRO TO ONION ROOT ACTIVITY-READ THROUGH before STARTING ACTIVITY

-MITOSIS IN WHITE FISH & ONION ROOTS-DETAILED SKETCHES! (take your time)

-use color when necessary [purple]

-CELLS ALIVE-mitosis tutorial [play animation]-cell cycle [basic animation / checkpoints]-cell cam [cancer / bacteria ]-cell quiz-cell gallerycheck out MEIOSIS

MITOSIS INTERNET LESSONRED PEN OUT

PRELAB: MITOSIS INTERNET LESSONONION ROOT TIP CLASSIFICATION

20 10 3 2 1

56 28 8 6 2

PRELAB: MITOSIS INTERNET LESSONMITOSIS IN WHITEFISH & ONION ROOT

a- Why is the whitefish used to study mitosis?-CELLS RAPIDLY DIVIDE

b- What are the four stages of mitosis?-PRO / META / ANA / TELO

c- How long does it take for mitosis to complete? -SEVERAL HOURS

d- Why will most of the cells you view be in interphase?

-LONGEST STAGE-grow/develop [metabolism]-replicate DNA-prepare

PRELAB: MITOSIS INTERNET LESSONMITOSIS IN WHITEFISH

ANAPHASE METAPHASE PROPHASE TELOPHASE

PRELAB: MITOSIS INTERNET LESSONMITOSIS IN ONION ROOT

ANAPHASE METAPHASEPROPHASE TELOPHASEINTERPHASE

LAB: OBSERVING MITOSIS/ONION ROOT-DETAILED SKETCHES! (take your time)-be NEAT: final copy

READ DIRECTIONS

-you may OBSERVE other slidesWHEN LAB IS COMPLETED

LAB: MITOSIS ONION ROOTPROBLEM/QUESTION: -Identify each stage of mitosis in a prepared slide of onion (allium) root tips. BACKGROUND: -In a growing plant root, the cells at the tip of the root are constantly dividing to allow the root to grow. Because each cell divides independently of the others, a root tip contains cells at different stages of the cell cycle. This makes a root tip an excellent tissue to study the stages of cell division.-Remember, interphase is not technically a part of Mitosis, but it is a part of the cell cycle and many of the cells you will be observing are in interphase.

LAB: MITOSIS ONION ROOT

LAB: MITOSIS ONION ROOTPROCEDURE:1-START with the microscope on LOW POWER OBJECTIVE / DIAPHRAM open to widest setting.2-OBTAIN a prepared slide of an onion tip root.-Hold the slide up to the light to see the pointed ends of the root section.-This is the root tip where the cells were actively dividing.

3-PLACE the slide on the microscope stage with the root tips pointing AWAY from you.-Using the LOW-power objective to find a root tip and focus it with the COARSE adjust until clearly visible.-Just above the root “cap” is a region that contains many new small cells. -The larger cells of this region were in the process of dividing when the slide was made.-These are the cells you will be OBSERVING.-Center the image, the SWITCH TO HIGH POWER.

LAB: MITOSIS ONION ROOT4-OBSERVE the box-like cells that are arranged in rows.-Select one cell whose chromosomes are clearly visible.-If you need to change the focus when using the high power only use the FINE ADJUSTMENT.

DATA:5-SKETCH the cell that you selected in the circle below labeled A

6-SELECT (4) other cells whose internal appearances are DIFFERENT from each other and from sketch-A.

7-Looking along the rows of cells you SKETCHED, IDENTIFY what stage each cell is in. (see RESOURCE)

LAB: MITOSIS ONION ROOT

PRELAB: MITOSIS INTERNET LESSON

LAB: MITOSIS ONION ROOTRESOURCE- Use the photos below as a guide to help IDENTIFY each stage of your sketches.

CHROMATIN

CHROMATID

INTER

PRO META ANA TELO

PRELAB: MITOSIS INTERNET LESSON

LAB: MITOSIS ONION ROOT  

8-Use the data table to RECORD the number of cell that you OBSERVE in each of the stages.

LAB: MITOSIS ONION ROOTCONCLUSION1-What STAGE were the MAJORITY of the cells in? -INTERPHASE 2-What EVIDENCE shows that mitosis is a CONTINUOUS PROCESS, not a series of separate events?-stages FLOW / BLEND into each other  

LAB: MITOSIS ONION ROOT3-SUMMARIZE what happens in each phase of MITOSIS: (use bullets)

PROPHASE-

METAPHASE-

ANAPHASE-TELOPHASE-

4-What is the end RESULT of mitosis AND why is it so IMPORTANT?  - (result) 

- (important/function)

Mitosis begins- chromosomes CONDENSE [chromatid]

Centrioles appear

Spindle fibers form

Chromatids attach to spindle fibers in MIDDLE

Chromatids separate- AWAY

2 new nuclei form

Mitosis ends

NEW CELLS [identical daughter cells]

REPAIR / REPLACE / GROW