Producción de compuestos de valor añadido en plantas biofactoría

Diego Orzáez

Jornada ASEBIO-INVEGEN Madrid Feb 2020

…more productive plants making healthier food with less resources

200 employees42 Staff Researchers

144 Pre- and Post-doctoral researchers2 main Public Spanish Research Institutions together

with 1 common objective…

Mechanisms of stress responseDevelopment and hormone actionMolecular virologyBreeding and biotechnology

years of Plant Research 25General Research Lines

Instituto de Biología Molecular y Celular de Plantas

EXCELLENT BASIC and TRANSLATIONAL RESEARCH

54 PhD thesis (5 years)136 total publications in 2017

(half in top D1 journals) 12 national and international patents

SUCCESS IN RAISING PUBLIC FUNDING (active in 2018)

8 EU projects (3 coordinated) 26 National MICINN grants

6 Regional GVA projects7 Young research projects

Studying the secret life of plants

FRUITFUL PUBLIC-PRIVATE PARTNERSHIP

Trusted by >25 industrial partners13 industry-led grants

ADVANCED RESEARCH SERVICES

Metabolomics, Genomics, Proteomics, DNA sequencing Microscopy and

Bioinformatics

QUALITY in EDUCATION and ENGAGEMENT with SOCIETY

PhD program in Graduate School UPVQuality awarded MsC in Plant Biotech>250/Y visits from secondary schools

LEADING EDGE SCIENTIFIC FACILITIES

7000 m2 labs and core services4150 m2 greenhouses and growth chambers

Unveiling the molecules that feed the world

Plant Biofactories

Food products from plants

FOOD VALUE: color, flavor, texture, nutritional value, health effectsIt is all about composition

Plant Biofactories

Non-Food products from plantsIndustrial crops

Biofuels Pharmaceuticals Fine chemicals Polymers

INDUSTRIAL VALUEIt is all about composition

Plant Biofactories

Low price agro products Unsustainable manufacturing systems

Can Plant Biotechnology contribute to:• Add value to agronomic products?• Increase manufacturing sustainability?

PLANT BIOFACTORIESAdding Value to Plant Composition through Biotechnology

Genetics determines composition

Plant Biofactories

Nicotiana benthamiana

Plant Biofactories

Nicotiana benthamiana

Plant Biofactories

Ingeniería Genética

avanzada (SynBio)

PLANTAS BIOFACTORIA

Técnicas avanzadas de

editado genético

Alimentos OMG

Mejora NPBT

Plan Biofactories

CRISPR

Guide RNA20 nucleotides

CRISPR: a revolution made in Alicante

Plant Biofactories

IBMCP Nicotiana benthamiana facilities

Cabinas cerradas

Hidroponía en invernadero

Sistemas agroinfiltración a vacío

Inoculación por spray

Plantas Biofactoría

Producción de compuestos de valor añadido en plantas

PRODUCTOS• Factores de crecimiento• Anticuerpos monoclonales• Nanoanticuerpos• Anticuerpos policlonales recombinantes

(tecnología patentada)• Péptidos/proteínas recombinantes a

demanda

Expresión transitoria enNicotiana benthamiana

CARACTERISTICAS• Rápido: 7-14 días• Animal-free• Altos rendimientos (sistemas virales) • Escalado a nivel mg-g

Start: 01/01/2018End: 30/06/2022Coordinator CSIC

Newcotiana

Plantas Biofactoría

Nicotiana tabacum Nicotiana benthamiana

NewcotianaAccelerated breeding of Nicotiana biofactories with New Plant Breeding Techniques

Plantas Biofactoría

Metabolite composition Growth habits

SAFE-SEX Biomass

Plantas Biofactoría

Newcotiana

Growth habits

SAFE-SEX Biomass

Plantas Biofactoría

Plantas Biofactoría

Metabolite composition

$$

Start: 01/01/2018End: 30/06/2022Coordinator CSIC

Newcotiana

Plantas Biofactoría

Plantas Biofactoría

• Plantas Biofactoria avanzadas• Productos Pharma • Colaboraciones directas empresa-

academia:

• Anticuerpos para kit de diagnóstico de enfermedad autoinmune raras(Diamante)

• Optimización de cultivos de raíces comoplataforma de producción en Biopharma (Samabriva)

H2020 (IA) Pharma-Factory

Plantas Biofactoría

ERA-CobiotechSUSPHIRE

• Producción sostenible de feromonas para protección de cultivos frente a insectos.

• Ingeniería metabólica de rutas de biosíntesisde feromonas en plantas y hongos.

• Cinco grupos investigación + SME, tres paísesUE, 1.8 M€

Gracias por su atención

Plantas Biofactoría

PLANT BIOFACTORIESAdding Value to Plant Composition through Biotechnology