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PRESENTATION DU PROJET ERC REVOLINCJeremy BOUYER Thierry BALDETUMR ASTRE Cirad/INRA
Journées EMBA, 13 juin 2018 UM
Toxicity and ecotoxicity of insecticides
Reduction of authorizedinsecticides
(by 75% in Europe)
Impact of VectorControl?
Insecticide resistance
Biologicalinvasions
Vectors/PestsPathogens
Environmentalchanges
(local/global)
Diseases outbreaksNuisance
Health impactEconomical loses
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THE CHALLENGE
Strong Need for INNOVATION
Source: Justin McBeath ISNTD Bites – March 19th 2015
… worse in Europe!
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DDT
Pyrethroids
Pyrethroids
Pyrethroids
SIT Boosted SITBouyer & Lefrançois Trends Parasitol 2014
THE PRINCIPLE
INSECT MODELS
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MosquitoesAedes albopictus
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PUBLIC HEALTH
2010 2015
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An invasive species in EuropeNuisance in Late Summer & AutumnRisk of Oubreaks of Aedes-borne viral diseasesChikungunya : Italy (2007, 2017) & France (2010 Var, 2014 Herault, 2017 Var) Dengue Croatia (2010) & France (2010 Nice, 2014 Var+BdR, 2015 Gard)
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Moustique tigre: « Appel à la vigilance » dans 42 départements métropolitains il y a 4 jours par la Direction Général de la Santé
Situation au 1er Janvier 2018
« surveillance renforcée »
Tsetse fliesGlossina palpalis gambiensis
Fruit fliesCeratitis capitata
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AGRICULTURE PUBLIC & ANIMAL HEALTH
Other Targets
VectopoleSud
LISIS
.
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Research Partners
WHAT IS NEEDED TO RUN BOOSTED SIT?WHAT WILL WE ADDRESS?
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Contamination technique of males
Resilience on males & transfer to females during mating
Impact on female fertility (+ transfer to breeding sites)
+
Transfer of biocides during matingand impact on female fertility
(PP & Densovirus)
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Pipeline of R & D for vector control tools/strategies
Source: B. Mosqueira
Laboratory Evaluation ISurvival of sterile males
of Glossina palpalis gambiensistreated with a new formulation of PP (F15)
0
10
20
30
40
50
60
70
80
90
100
0H 6H 48H 4j 7j 9j 13j 15j 17j 21j
Su
rviv
alo
f st
eril
em
ales
(%
)
Time after treatment
Master2 Lison LarocheNo impact of PP (F15) on male survival
Control F15
35mg F15 + Pyriproxyfen
Control
Laboratory Evaluation IIPersistency of PP (F15) on treated males
of Glossina palpalis gambiensis
0
200
400
600
800
1000
1200
1400
Am
ou
nt
of
PP
per
tse
tse
(ng
)
Time after treatment
0H 3H 6H 24H 48H 72H 4J 6J 8J 10J
Fast initial decline of PP followed by a level off around 100ng during 10 days
Master2 Lison Laroche
1,35
15,35 17,82 17,65 18,26
42,78
71.45
0
10
20
30
40
50
60
70
80A
mount
of
py
ripro
xy
fen
per
fem
ale
(ng)
Nb of contacts without mating
Laboratory Evaluation IIITransfer of PP from males* to females
of Glossina palpalis gambiensis
Master2 Lison Laroche
*3h after treatment by PP (F15)
To be followed: Impact of PP transferred on female fertility…
Laboratory Evaluation ISurvival of males of Aedes albopictus
treated with a new formulation of PP (F15)
Reduced survival of treated males (+50% mortality in 14 days)
183,48
132,01
107,67
62,01
42,03
16,76
-40
0
40
80
120
160
200
240
T0 (0 heure) T1 (3heures)
T2 (6heures)
T3 (24heures)
T4 (48heures)
T5 (4 jours)
Am
ou
nt
of
PP
pe
r in
div
idu
al (
ng)
Time after treatment
Laboratory Evaluation IIPersistency of PP (F15) on treated males
of Aedes albopictus
What about associating SIT to DENSOVIRUSES?
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Drawn by Steve Higgs 19
Copyright © 2016 Bouyer et al. Open Access article distributed under the terms of CC BY-NC-ND Terms and Conditions
The Lancet Global Health 2016 4, DOI: (10.1016/S2214-109X(16)00082-6)
Mosquito DensovirusesHigh specificity
Carlson et al. 2006 Advances in virus research
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Mosquito DensovirusesOn-going trials
DVs moustiques Origine des DVs
Production en cellules
(virus équivalent génome –veg/µl) sécrétion dans le
surnageant
Test d’infectionen larves
Aedes albopictus
Densovirus 2
AalDV2
C6/36P chroniquement infectées
Jousset et al., 1993
Récolte des surnageants de culture
2,4 x 108 En cours
Aedes aegypti
Densovirus 1
AaeDV1
Clone viral origine: synthèse de
genes
Production de virus par transfection de culture de
cellules de moustiques
C6/36 3,4x106
AAG2 1,2x103
Clone viral origine : clonage génome
viral
Production de virus par transfection de cultures
de cellules de moustiques
C6/36 1,3 x 101
AAG2 3,8x106 En cours
Survival curve for Ae. albopictus larvae (Italian strain)
infected with AalDV2 virus
Survival curve for Ae. aegypti larvae (Bora strain)
infected with AalDV2 virus
Protocol:
• 1 larvae L1, P96 well, +/- virus
• Transfert 48hpi in P24 well plate + food
Protocol:
• 1 larvae L1, P96 well, +/- virus
• Virus +/- FB28 0,1%
• Transfert 48hpi in P24 well plate + food
Aedes albopictus Densovirus 2 produced from infectious plasmid Infection in Aedes albopictus and Aedes aegypti larvae
Aedes albopictus Densovirus 2 producedfrom C6/36 chronically infected cell line
Infection in Aedes albopictus larvae
Ind
ivid
ual
s n
um
ber
at
14
dp
i
Protocol:
• 100 larvaes L1, 5 ml cell culture
• Transfert 48hpi in 300 ml water + food
• Analysis 14 dpi AalDV2 effects : • ↗ mortality• ↘ development• ↗ cannibalism
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Semi-field trials Experimental field trials
Impact of boosted SIT in semi-field and field trials with the best biocide
(Ae. albopictus & C. capitata)
Deterministic models Individual-based models
Relative impacts of SIT and boosted-SIT on population dynamics and resilience
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Preliminary models with Pyriproxyfen
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Elimination with minimised Rtotal: RSIT opt = 2528RBSIT opt = 1214
David Pleydell
Preliminary models with Pyriproxyfen
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Modèle
Observation
Spatial modelAccounting for ClimateValidated on entomological dataUsed by Agence Régionale de Santé (ARS) to target monitoring and control
Population dynamics of Ae. albopictus in La Réunion (AlboRun)
PhD Marion Haramboure
First results of integrating SIT to the model
Perspectives
200,000 sterile males released weekly for 3 months
With a constant climate, return to equilibrium only 1.5 month after releases
Boosted SITStudy various release scenariosImpact of Climate on vector control
PhD Marion Haramboure
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Mass rearing and irradiation procedures
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Sexing of mosquitoes and handling procedures
2 genetic strategies to be compared to Tragsa Laser sorter:- Development of non-transgenic GSS- RNAi sexing
Quality control of produced strains
Sex separation method (female elimination) and quality control
PhD student (Start in July 2018)
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Development of an automatic release machine
Photo WeRobotics
Successful trial of the drone release system in Brazil on Aedes aegypti in March 2018 (https://www.iaea.org/newscenter/pressreleases/iaea-conducts-successful-test-of-drones-in-fight-against-disease-transmitting-mosquitos)
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Risk assessment Regulatory issues
Regulatory issues and social acceptability of boosted SIT
Social acceptability
Review of regulatory issues, technical constraints and social acceptability of genetic control in Europe
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Revolinc & Regulatory issues
Audition des expertsSaisines en phase finale de rédactionPrésentation des saisines pour décision auprès HCSP & AFB (fin juin 2018)
Revolinc & social sciences
• La Reunion island
• 29 Jan - 16 Feb. 2018
• Survey by observations
• Meeting of scientific actors from CIRAD, Revolinc, IRD, TIS-La Réunion, ARS and collaborators on CYROI platform of Saint-Denis in the context of phase 2 preparation meeting of TIS-project
PhD Kamil Ghouati
• Vienna, Seibersdorf(Austria)
• 26 - 29 mar. 2018
• Survey by interviews & observations
• Meeting of members fromInsect Pest Control Lab ( FAO / IAEA Programme on Nuclear Techniques in Food and Agriculture )
Revolinc & social sciences
PhD Kamil Ghouati
• Vienna, Seibersdorf (Austria)
• 23 - 30 April 2018
• Survey by interviews & observations
• Meeting with European stakeholders in charge of mosquito control during a meeting on stakeholder engagement in mosquito SIT projects organized by IAEA
Revolinc & social sciences
PhD Kamil Ghouati
1. Identification of actors in the different sites: project leaders, stakeholders, collaborators, funding agencies, civil society, public(s), beneficiaries…
2. Identification of actors logics, categories involved to account for the action & social dimension of SIT projects, on scientific & technical thematics in the society, on technical democracy and on research participation: education, sensitization, communication, vulgarization, etc.
3. Study of social relationships and their dynamics along with ongoing projects deployment
Revolinc & social sciencesPreliminary results
PhD Kamil Ghouati
Thanks!
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