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Principles of DiseasePrinciples of DiseaseMicrobiology 2314
• Every disease is a race between a pathogen trying to gain a foothold and the host defenses trying to prevent the pathogen from doing so.
DiseaseDisease
• Is a Process
• Is a State of Being Not In Good Health
• Involves Many Factors
- Age
- Nutrition
- Gender
- Race
• We tend to equate one pathogen with one disease, but it is more complicated than that.
• It is actually much more complicated than that.
Definitions: ParasiteDefinitions: Parasite
• Refers to Protozoans and Worms
Definition: PathogenDefinition: Pathogen
• Refers to Bacteria / Virus / Fungus
• An organism with the potential to cause disease.
• Infection
A pathogen growing in or on the host
• Virulence
The degree or intensity of pathogenicity
• Invasiveness
Ability to spread to other tissues
• Infectivity
Ability to secrete toxins
• Septicemia
Blood infection
More Definitions:More Definitions:
• Pathology
Scientific study of disease• Etiology
Cause of disease• Pathogenesis
Development of disease• Host
Organism that shelters and
supports the growth of
pathogens.
DiseaseDisease• An abnormal process in which part or all
of the body is not properly adjusted or is incapable of performing normal functions often because of infection by pathogens.
Relationships Between Relationships Between Microorganisms and ManMicroorganisms and Man
David Vetter the “Bubble Boy”
Usually germ-free in utero
Microorganisms begin colonization in and on the surface of the body during and after birth
We require these organisms
Germ-free organisms are less healthy than organisms with normal microbiota
Body Regions Have Characteristic Body Regions Have Characteristic FloraFlora
• Skin 1012
• Mouth 1010
• GI Tract 1014
Normal Flora Are Found MostlyNormal Flora Are Found Mostly
– on the skin – in the eyes – in the nose – in the mouth – in the upper throat – in the lower urethra – in the lower intestine – especially in the large intestine
• Note that this list basically includes all of the body surfaces exposed to the external environment.
Microbial AntagonismMicrobial Antagonism• Normal Microbiota establish permanent colonies
inside or on the body without producing disease• Symbiosis• Three Types
1. Commensalism
2. Mutualism
3. Parasitism• Transient microbiota are members of the normal flora
that are not always present or are present for only a few days, weeks, or months before disappearing.
Nitrogen Fixation -- MutualismNitrogen Fixation -- Mutualism
Ruminants and Resident Microbes – MutualismRuminants and Resident Microbes – Mutualism
Lichen Symbiosis Lichen Symbiosis
-- Mutualism-- Mutualism
Ascaris Worms / Ascaris Worms / ParasitismParasitism
• The upper respiratory tract consists of the nostrils, nasal cavities and throat. It contains a number of commensalistic inhabitants including: Staphylococcus epidermidis.
Out of all types of symbiotic bacteria, commemsalists are the least studied. The reason for this is simple; if a bacteria is not causing harm or benefit to a host there is simply not much reasons to studying it.
Microbial AntagonismMicrobial AntagonismMicrobial Antagonism or Colonization inhibition: A process by which pathogenic microorganisms are inhibited by normal florafrom colonizing healthy organisms (a form of symbiosis)
Antagonism between the fungus Paraconyothyrium variabile and Fusarium oxysporum
• Mechanisms by which this inhibition occurs includes: 1. competing with pathogenic microorganisms
for nutrients
2. competing with pathogenic microorganisms for space
3. producing toxins that are harmful to some pathogenic microorganisms
Remember!Remember!
• Categorizing symbiotic relationships is convenient, but keep in mind that under certain circumstances the relationship can change.
Question?
• A colonic microorganism which bores into its host and by doing so does it damage, competes with its host for nutrients, but is the sole supplier of an organic growth factor without which the host could not survive. This symbiotic relationship is an example of (chose the best answer):
a. commensalism b. mutualismc. parasitism
Question?
• A colonic microorganism which bores into its host and by doing so does it damage, competes with its host for nutrients, and is the sole supplier of an organic growth factor without which the host could not survive. This symbiotic relationship is an example of (chose the best answer):
a. commensalism b. mutualismc. parasitism
Question?
• A bacteria is attached to the intestinal wall via its pili, causes its host no harm, and secretes an antibacterial poison which limits colonization of the intestine by other, unrelated bacteria. With regard to the prevention of disease, what process specifically is this bacteria effecting?
Microbial Antagonism
Question?
• Microbial antagonism (choose best answer) a. is a parasitic interaction between two
organisms b. describes generally the harm done by a
microorganism to a host c. can be an example of mutualism d. can be an example of parasitism e. all of the above Ab. none of the above
Question?
• Microbial antagonism (choose best answer) a. is a parasitic interaction between two
organisms b. describes generally the harm done by a
microorganism to a host c. can be an example of mutualism d. can be an example of parasitism e. all of the above Ab. none of the above
Question?
• A colonic microorganism which bores into its host and by doing so does it damage. This organism has no additional redeeming characteristics. You would describe the symbiotic relationship it has with its host as an example of (choose the best answer):
a. commensalism b. mutualism c. microbial antagonism d. parasitism e. none of the above
Question?
• A colonic microorganism which bores into its host and by doing so does it damage. This organism has no additional redeeming characteristics. You would describe the symbiotic relationship it has with its host as an example of (choose the best answer):
a. commensalism b. mutualism c. an effector of microbial antagonism d. parasitism e. none of the above
Question?
• Which is not a member of the normal flora of a plant or an animal? (assume all are obligate colonizers of the plant or animal host organism)
a. a bacterium b. a virus c. a fungus d. a protozoa e. all are equally likely to be members of normal flora
Question?
• Which is not a member of the normal flora of a plant or an animal?
a. a bacterium b. a virus c. a fungus d. a protozoa e. all are equally likely to be
members of normal flora
Question?
• Name a part of the body that you would not expect to have an associated normal flora.
StomachKidneyLiverEtc.
Anything Basically That Is Internal
• Transient microbiota are members of the normal flora that are not always present or are present for only a few days, weeks, or months before disappearing.
Transient MicrobiotaTransient Microbiota
Opportunistic MicroorganismsOpportunistic Microorganisms• Do not cause disease under normal conditions but
can cause disease under special conditions.
Staph Infection
Cooperation Among Cooperation Among MicroorganismsMicroorganisms
• One of the organizing principles of life on Earth is that cells cooperate.
• This is evident in the case of multicellular organisms, from nematodes to humans, but it also appears to apply widely among single-celled organisms such as bacteria, fungi, and amoeba.
• A well-studied example of intraspecies cooperation concerns the cyanobacterium Anabaena, which grows in long chains, in which approximately one cell out of ten differentiates into a heterocyst that provides fixed nitrogen for the neighboring cells
This Cellular Cooperation Can This Cellular Cooperation Can Increase the Virulence of a Increase the Virulence of a
MicroorganismMicroorganism
Example:
Strep Infection Scarlet Fever Rheumatic Fever
The Etiology of Infectious DiseaseThe Etiology of Infectious DiseaseKoch’s Postulates Koch’s Postulates
• Robert Koch played an important role in determining that specific microbes were associated with specific diseases.
• Four Postulates
1. Same pathogen must be present in every case of the disease.
2. Pathogen must be isolated in pure culture.
3. Pathogen isolated from pure culture must cause the same disease in lab animal.
4. Pathogen must be re-isolated from inoculated lab animal.
Exceptions to Koch’s PostulatesExceptions to Koch’s Postulates
• Inability to grow on artificial media (Syphilis)
Exceptions to Koch’s PostulatesExceptions to Koch’s Postulates
• Unequivocal signs and symptoms (Tetanus)
Exceptions to Koch’s PostulatesExceptions to Koch’s Postulates
• Diseases caused by variety of organisms (Pneumonia)
• Pathogens causing variety of diseases (Streptococcus pyogenes)
Exceptions to Koch’s PostulatesExceptions to Koch’s Postulates• Diseases that occur in humans only (Smallpox)
Classifying Infectious DiseasesClassifying Infectious Diseases
• Symptoms
Subjective Changes
Cannot Be Measured
• Signs
Objective Changes
Measurable Changes
Sign or Symptom?Sign or Symptom?
• Pain
• Temperature
• Nausea
• Swelling
• Discomfort
• Blood Pressure
Classifying Infectious DiseasesClassifying Infectious Diseases• Syndrome
A specific group of symptoms or signs
that always accompany a specific disease.
What is the Syndrome?What is the Syndrome?
• Flu• Measles• Anthrax• Legionnaires• Mumps• Carbuncles• Smallpox• West Nile Virus
Communicable DiseasesCommunicable Diseases• Transmitted Directly or Indirectly From
One Host to Another Host
Chickenpox
Measles
Genital Herpes
Contagious DiseasesContagious Diseases• A communicable disease that is spread
easily from one individual to another
Name a Communicable Disease that is Not Contagious.
Noncontagious DiseasesNoncontagious Diseases
• Caused by microorganisms that normally grow outside the human body and are not transmitted from one host to another.
Tetanus
Anthrax
The Occurrence of DiseaseThe Occurrence of Disease• Incidence
Number of New Cases of Disease in a
Given Population.
The Risk of Disease• Prevalence
Number of Existing Cases of Disease in
a Given Population at Any One Time
The Burden of Disease
Question?
• Assume a classroom of 50 students exposed to a new strain of three-day influenza.
• Before exposure, the prevalence and incidence in this population are both zero.
• If within one week, 5 out of the 50 students contract the flu, what is the incidence?
• What is the prevalence for that one week period?
Continue?
• If after one more week, 5 more students become infected, what is the point prevalence?
• What is the overall prevalence?
• What is the incidence?
Trends
• Changes in incidence and prevalence are usually followed over a seasonal, yearly, and long-term basis and are helpful in predicting trends.
• Statistics of concern to epidemiologists are the rates of disease with regard to sex, race, and geographic region.
What We SeeWhat We See
• Infectious Diseases
High Incidence
Low Prevalence
• Chronic Diseases
Low Incidence
High Prevalence
Classification of DiseaseClassification of Disease
• Sporadic
Only an Occasional Case (Typhoid Fever)• Endemic
Constantly Present at an Expected Level (Common Cold)
• Epidemic
Above the Normal Expected Frequency (Flu)
• Pandemic
World Wide Epidemic (AIDs)
Scope of DiseaseScope of Disease
• Acute
Develops Rapidly
Short Lifespan
Usually Severe Symptoms
Example: Flu, Measles
• Chronic
Develops Slowly
Continual, Long Lasting, or Reoccurring
Usually Less Severe
Example: Osteoporosis
• Latent
Causative Agent Remains Inactive for a
Time – Then Becomes Active and
Produces Symptoms.
Chicken Pox to Shingles
HIV to AIDS
Herd Herd ImmunityImmunity
• The presence of immunity to a disease in most of the population.
• Normally due to Vaccination.
Herd Immunity is the Reason we Vaccinate!
The way herd immunity works is by isolating potential hosts and reducing virus’ opportunities to be passed on. For herd immunity to be effective I need to vaccinate at least 90% of the susceptible population.
If I drop below a 90% vaccination rate, the chances of passing infection increase and I get small clusters of disease occurring.
Emerging Infectious DiseasesEmerging Infectious Diseases
• New diseases with increasing incidence.
1. SARS
2. Ebola
3. West Nile Virus
4. Bird Flu
5. Monkeypox
Emerging Infectious DiseasesEmerging Infectious DiseasesEIDsEIDs
• EIDs Can Result From
1. Overuse of Antibiotics
2. Overuse of Pesticides
3. Climatic Changes
4. Travel
5. Lack of Necessary Vaccinations
The CDC, NIH and WHO are responsible for surveillance and responses to EIDs and the world’s health.
Re-emerging Infectious DiseasesRe-emerging Infectious DiseasesRIDsRIDs
• Old diseases that are occurring with increasing incidence.
1. Tuberculosis
2. Hantavirus
3. Plague
The Extent of Host Involvement The Extent of Host Involvement Local InfectionLocal Infection
• A local infection affects a small area of body
• Boil or Acne
Systemic InfectionSystemic Infection• Infection spread throughout the body via the
circulatory system• Mumps • Systemic Infections often lead to Focal Infections.• What is a Focal Infection?
Secondary InfectionSecondary Infection
• After the host is weakened from a primary infection, it is easier for them to become infected with a secondary infection due to a compromised immune system.
Unapparent or Subclinical InfectionUnapparent or Subclinical Infection• An apparently healthy individual carries the disease
organism and passes it on to others without expressing any symptoms of the illness.
• Poliovirus• Hepatitis A• HIV
Reservoirs of Reservoirs of InfectionInfection
For a disease to perpetuate itself, there must be continual organisms available and a source providing appropriate conditions.
Birds are Reservoirs for WNV
ZoonosesZoonoses
• Diseases that affect wild and domestic animals and can be transmitted to humans.
• Lyme Disease
• Rabies
• Anthrax
• West Nile Virus
Transmission of ZoonosesTransmission of Zoonoses• Direct Contact with Infected Animals
• Direct Contact with Animal Waste
• Contamination of Food and Water
• Contaminated Hides, Fur, and Feathers
• Consuming Infected Animal Products
• Insect Vectors
Some Diseases Result from Pathogenic Some Diseases Result from Pathogenic Microorganisms Growing in or on Microorganisms Growing in or on
Nonliving Reservoirs Such as Soil and Nonliving Reservoirs Such as Soil and Water or ObjectsWater or Objects
• Ringworm
• Botulism
• Tetanus
Transmission of Transmission of DiseaseDisease
• Direct Transmission Indirect Transmission1. Close Personal Contact 1. Fomites
2. Touching, Kissing, etc. 2. Droplets/Airborne
3. Vehicles
4. Vectors
Direct Transmission
1. Personal Contact
2.Touching
Kissing
Sex
Etc.
Indirect TransmissionIndirect Transmission
• Fomites
Inanimate Objects
Tissues, Drinking Glasses, Needles
AIDS, Hepatitis
Indirect TransmissionIndirect Transmission• Transmission by a Medium
Water, Food, Air
Plague, Cholera
Indirect TransmissionIndirect Transmission
• Droplet Transmission
Saliva and Mucus
Coughing, Sneezing, Laughter
Less than 1 Meter
Indirect TransmissionIndirect Transmission
• Airborne Transmission
Carried on Water/Mucus Droplets
Greater than 1 Meter
Indirect TransmissionIndirect Transmission
• Vectors (Often Arthropod)1. Mechanical Means
Passive ProcessPathogens are on the body of the vector.Ex. Fly landing on food
Can you name a Disease Transmitted by this this Process?
Indirect Indirect Transmission Transmission
• Vectors (Often Arthropod)
2. Biological Means Active Process Vector must bite or scratch or sting. Ex. Mosquito Bite
Can you name a Disease Transmitted
by this process?
Routes of TransmissionRoutes of Transmission1. Vertical
Mother to Offspring- Transplacental: Rubella, Toxoplasma (Note: Humans only have two layers of
separation)- Perinatal: Listeria, Gonorrhea, Chlamydia- Breastfeeding: HIV
2. Horizontal Person to Person
- Contact Spread- Common Vehicle- Airborne- Vector-borne
Portals of Exit (Preferred Ways to Leave Portals of Exit (Preferred Ways to Leave the Body and Spread Infection)the Body and Spread Infection)
• Respiratory Tract
(Coughing or Sneezing)• Gastrointestinal Tract
(Saliva or Feces)• Urogenital Tract
(Secretions from the Vagina or Penis)
Arthropods and Syringes provide a portal of exit for microbes in blood.
Nosocomial InfectionsNosocomial Infections
• An infection that is acquired during the course of stay in a health facility.
• 2 million NI Yearly• 5-15% of All
Patients Acquire NI• 90,000 Die
The Rate of Nosocomial Infections Is Increasing
Trends in Nosocomial InfectionsTrends in Nosocomial Infections
• 1940’s – 1950’s Gram + S. aureus
• 1970’s Gram – E. coli
• 1980’s Gram + A.R. S. aureus
• 1990’s Both
• 2000’s No Relief in Sight
Frequency of Nosocomial Frequency of Nosocomial InfectionsInfections
Large Number of Skin Infections Due to Newborns
The Compromised Host is Most The Compromised Host is Most at Riskat Risk
1. Burns (Broken Skin)
2. Surgical Wounds (Broken Skin)
3. Suppressed Immune Systems (Due to Ill Health)
The Body Defends UsThe Body Defends Us
• Skin and Mucus Membranes• T-Lymphocytes Kill Pathogens• B-Lymphocytes Produce Antibodies
• Their Effectiveness if Altered By Many Things
- Drugs- Radiation- Steroids- Burns- Diabetes- Stress- Poor Nutrition
Chain of Transmission:Chain of Transmission:Nosocomial Infections Are Transmitted Nosocomial Infections Are Transmitted
By Direct Contact Between Staff By Direct Contact Between Staff Members and PatientsMembers and Patients
Fomites such as catheters, syringes, and respiratory devices can transmit nosocomial infections.
Control of Nosocomial InfectionsControl of Nosocomial Infections
1. Handwashing #1
2. Disinfect Equipment
3. Necessary Antibiotics Only
4. Infection Control Team
Could a copper door handle help to beat MRSA (2009 Study)?
• Doorknobs, bath taps, toilet handles and 'grab rails' will be ripped out and replaced with copper versions at Birmingham's Selly Oak hospital in a bid to beat MRSA
• MRSA has proven resistant to even the most powerful antibiotics. But hospital superbugs may finally have met their match - in copper door handles.
• Copper, used in medicines for 4,000 years, has been shown to be highly effective in killing off pathogens such as MRSA.
• In the trial study, copper grew 95% less bacteria.
Predisposing FactorsPredisposing Factors
• Gender
• Climate
• Age
• Fatigue
• Poor Nutrition
• Low Vitamin D
A Factor that Makes the Body More Susceptible A Factor that Makes the Body More Susceptible to Disease or Alters the Course of an Illness.to Disease or Alters the Course of an Illness.
EpidemiologyEpidemiology
• The study of the distribution and determination of health related events within a given population.
• The study of the transmission, incidence, and frequency of disease
1. Descriptive Epidemiology
2. Analytical Epidemiology
3. Experimental Epidemiology
Descriptive EpidemiologyDescriptive Epidemiology• Data about infected people are collected
and analyzed in descriptive epidemiology.
• Who, What, When, Where, How, etc.
Analytical EpidemiologyAnalytical Epidemiology
• A group of infected people is compared with an uninfected group.
• Analytical epidemiology aims to research and study risk and protector factors of diseases.
Relative Risk of Death on the Relative Risk of Death on the TitanicTitanic
308 154
142 709
Alive Dead
Female
Male
462
851
308 154
142 709
Alive Dead
Female
Male
462
851
Probability of Death for Females is 154 / 462 = .333 or 33%
Probability of Death for Males is 709 / 851 =.833 or 83%
RR = .833 / .333 = 2.5
There is a 2.5 greater probability of death for males than for females.
Experimental EpidemiologyExperimental Epidemiology• Use of controlled experiments designed to test
hypotheses are performed in experimental epidemiology.
• Example: In these studies normal guinea pigs were exposed to tuberculosis cage mates in two different degrees of crowding, some in ordinary cages, where the food became contaminated with the excreta, laden with tubercle bacilli, of the inoculated animals, and some in special cages with wire-mesh floors, where these excreta were largely excluded as a source of contagion.
Clinical TrialClinical Trial
HistoryHistory
• John Snow
• London Cholera Epidemic
• Broadstreet Pump
The CDC is the Main Source of The CDC is the Main Source of Epidemiological Information in the Epidemiological Information in the
United StatesUnited States