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Program in NHP Program in NHP NeuroimagingNeuroimaging
Calum AvisonCalum Avison
OverviewOverview RationaleRationale
BiophysicsBiophysics Biology / behaviorBiology / behavior
Facilities / CapabilitiesFacilities / Capabilities ImagingImaging Support EquipmentSupport Equipment StaffStaff
ProjectsProjects DTI Tractography ValidationDTI Tractography Validation Rhesus (3T) - neurodevelopmental consequences of cocaine exposure Rhesus (3T) - neurodevelopmental consequences of cocaine exposure in in
uteroutero Squirrel monkey (9.4T) - neural correlates of tactile illusion / correlation Squirrel monkey (9.4T) - neural correlates of tactile illusion / correlation
with optical imaging and electrophysiologywith optical imaging and electrophysiology
NHP Rationale - BiologyNHP Rationale - Biology
Structural Homology?Structural Homology? Functional Homology?Functional Homology?
Project 1 - Prenatal Cocaine Project 1 - Prenatal Cocaine Exposure (PNCE)Exposure (PNCE)
Prenatal Cocaine Exposure in RhesusPrenatal Cocaine Exposure in Rhesus
Developmental neurobiologyDevelopmental neurobiology PNCE PNCE behavioral/cognitive impairment? behavioral/cognitive impairment? StructureStructure Functional circuitryFunctional circuitry Pre-/post-synaptic defects in neurotransmissionPre-/post-synaptic defects in neurotransmission
MethodologyMethodology PET mapping of receptors, neurotransmitter release PET mapping of receptors, neurotransmitter release High resolution structural MRI + VBMHigh resolution structural MRI + VBM High resolution DTIHigh resolution DTI fMRI responses to pre-/post-synaptic agentsfMRI responses to pre-/post-synaptic agents Co-registrationCo-registration
Co-registered (ABA) PET/MR images Co-registered (ABA) PET/MR images of Rhesus Monkeyof Rhesus Monkey
T1-weighted MR image acquiredon a human 3T scanner
Summed image from a 3-hracquisition after 4.9 mCi injection of the D2 receptorligand [F-18]fallypride
HARDI / FORECAST HARDI / FORECAST - NHP (92 directions)- NHP (92 directions)
p_2_17p_2_22p_2_27p_2_31
1.5 mm isotropic2ch FLEX-M coilSENSE, R=1.5
DTI TractographyDTI Tractography
HARDI / FORECAST HARDI / FORECAST - NHP (92 directions)- NHP (92 directions)
p_2_17p_2_22p_2_27p_2_31
0.8 mm isotropic2ch FLEX-M coilSENSE
Implementation on Intera Achieva Implementation on Intera Achieva 3T 3T
Humans - 2 mm isotropic; 32, 92 directionsHumans - 2 mm isotropic; 32, 92 directions Neonatal developmentNeonatal development
Normal and abnormalNormal and abnormal Developmental abnormalitiesDevelopmental abnormalities Correlation with ss-fMRI (structure begets function)Correlation with ss-fMRI (structure begets function)
NHPs - 0.8 mm isotropic; 92 directionsNHPs - 0.8 mm isotropic; 92 directions Validation of HAR diffusion methodsValidation of HAR diffusion methods Structural homology (y/n?) with humansStructural homology (y/n?) with humans
y y Model of human brain Model of human brain n n Brain evolution Brain evolution
Effects of prenatal cocaine exposureEffects of prenatal cocaine exposure
HARDI / FORECAST HARDI / FORECAST - Human (92 directions)- Human (92 directions)
h_03
HARDI / FORECAST HARDI / FORECAST Human vs NHP (92 directions)Human vs NHP (92 directions)
h_03 p_2_17
HARDI / FORECAST HARDI / FORECAST Human vs NHP (92 directions)Human vs NHP (92 directions)
h_09 p_2_22
HARDI / FORECAST HARDI / FORECAST Human vs NHP (92 directions) Human vs NHP (92 directions)
h_10 p_2_25
NHP at 3TNHP at 3T
•2ch FLEX-M coil
•SENSE
NHP at 3TNHP at 3T
NHP at 3TNHP at 3T
NHP at 3TNHP at 3T
Coregistration
(Overlay)
functional 2D anatomic
Draw Volumes-of-interest
- Look at timecourse information for each VOI
- Statistics: GLM, ROI analysis
Coregistration using gyri information to match 2D and 3D
Volumes-of-interest
Putamen(Divided into RH and LH)
Caudate head(Divided into RH and LH)
supragenual anterior cingulate cortex (ACC1)
Subgenual anterior cingulate cortex (ACC2)
BrainVoyager Capability
Graph and Table of beta weight values
General Linear Model
Can apply statistics and obtain timecourse of only significantly activated voxels. (above: TC of significantly activated voxels of all six determined volumes-of-interest. 1.0 mg Cocaine administered at volume 200.)