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In vitro Assessment of Antimicrobial Activity of some
Ornamental Flowers
Presented by:Bidisha Ghosh8th Sem, B.Tech, Biotechnology, SITM
Under the supervision of:Mr. Neeraj Mishra
Asst. Prof. & Head(Dept. of Biotechnology), SITM
Infectious disease & traditional drug problems
Leading cause of premature deaths, killing almost 50,000 people/day
Just 4yrs after the start of mass-producing penicillin in 1943 resistant microbes began appearing
Development of resistance to common antibiotics in all major pathogens are reported
These negative health trends necessitated a search for new antimicrobial substances from other sources including plants.
Use of flowers as medicine
50% of pharmaceuticals originate from plants
Most plants contain bioactive compounds that have good antimicrobial activity
Plant antimicrobials are not associated with side effects
Some flowers with reported antimicrobial
activity:
ChrysanthemumJasminePoppyRosemaryThyme
Objective of project
Evaluating antimicrobial activity of water & alcohol extracts of 4 flowers against 5 microorganism by Agar Well Diffusion Method.
Flowers used
Cyme rose (Rosa indica)
Lotus(Nelumbo nucifera)
Orange Marigold(Tagetes erecta)
Yellow Red Marigold(Tagetes patula)
Preparation of flower extracts
Water
extract
5 g flower+
100 ml dist. water
Ran in soxhlet
(4 hrs at 100OC)
Alcoholextract
5 g flower+
100 ml alcohol
agitated for 8 hrs in a wrist-action
shaker
alcohol evaporated without heat & residue
mixed with 100 ml of dist water
Microorganisms analyzed
Bacillus subtilis
Staphylococcus aureus
Escherichia coli
Pseudomonas aeruginosa
Candida albicans
Analysis of antimicrobial activity
Method:
Agar Well Diffusion Method
Media:
Muller Hinton agar
Parameter:
Zone of Inhibition Measuring the inhibition zone
Antimicrobial activity against Staphylococcus aureus
Water extract
Alcohol extract
0
2
4
6
8
10
12
Bacillus subtilis Staphylococcusaureus
Escherichia coli Pseudomonasaeruginosa
Candida albicans
inhib
ition z
one(in m
m)
Rosa indica Nelumbo nucifera Tagetes erecta Tagetes patula Control
Graphical representation of antimicrobial activity with water extract
Orange Marigold showed activity against all five microorganisms
Rose gave the highest inhibition.
Candida albicans showed the highest resistance & was susceptible only to Orange Marigold & Yellow Red Marigold.
0
2
4
6
8
10
12
Bacillus subtilis Staphylococcusaureus
Escherichia coli Pseudomonasaeruginosa
Candida albicans
inhib
ition z
one (
in m
m)
Rosa indica Nelumbo nucifera Tagetes erecta Tagetes patula Control
Graphical representation of antimicrobial activity with alcohol extract
Yellow Red Marigold showed very high activity against all five
organism
Rose also showed strong inhibiting capability
Most resistant microorganism was again Candida albicans
ConclusionConclusion
Flower extracts have great potential as
antimicrobial compounds
Rosa indica & Tagetes patula showed
maximum antimicrobial activity
These flowers can be used to discover bioactive compounds that may serve as leads for development of new pharmaceuticals
AcknowledgementsAcknowledgements
Mr. Neeraj Mishra, Asst. Prof. & Head of the
Dept., Biotechnology, SITM.
Dr. Vinod Bihari, Director of Department of
Biotechnology All the faculty of the Dept., Biotechnology,
SITM. My parents.
ThankThank
YouYou