Proline Mechanical Eng

8/3/2019 Proline Mechanical Eng

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Proline

Kyttohje

Bruksanvisning

Instruction Manual


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Grip cover:metal blue 721060grey 721752yellow 721825red 721706blue 721221green 721792

Handle,complete, in gray colour0.1-2.5 l 7210610.5-10 l 7210622-20 l 7210635-50 l 72106410-100 l 72106520-200 l 72108250-200 l 721066100-1000 l 721067

200-1000 l 7210681-5 ml 721069Piston assembly:0.1-2.5 l 7211450.5-10 l 7211412-20 l 7211505-50 l 72115010-100 l 72116020-200 l 72116050-200 l 721160

100-1000 l 721175200-1000 l 7211751-5 mI 721173Piston:0.1-2.5 l 7211460.5-10 l 7211422-20 l 7211515-50 l 72115110-100 l 72116120-200 l 721161

50-200 l 721161100-1000 l 721176200-1000 l 7211761-5 ml 721177Piston spring:0.1-2.5 l 7211430.5-10 l 7211432-20 l 7211525-50 l, 72115210-100 l 721162

20-200 l 72116250-200 l 721162100-1000 l 721465200-1000 l 7214651-5 ml N/ASpring support:0.1-2.5 l 7211440.5-10 l 7211442-20 l N/A5-50 l N/A

10-100 l 72114420-200 l 72114450-200 l 721144100-1000 l 721464200-1000 l 7214641-5 mI N/AO-ring, set of 10 pieces0.1-2.5 l N/A0.5-10 l 7210902-20 l 7211005-50 l 72110010-100 l 72111020-200 l 72111050-200 l 721110100-1000 l 721120200-1000 l 7211201-5 ml 721121

Secondary spring:0.1-2.5 l N/A0.5-10 l N/A2-20 l N/A5-50 l N/A10-100 l N/A20-200 l N/A50-200 l N/A

100-1000 l 721463200-1000 l 7214631-5 mI N/ATip cone:

0.1-2.5 l 7210120.5-10 l 7210112-20 l 7210205-50 l 72102010-100 l 72103020-200 l 721030

50-200 l 721030100-1000 l 721041200-1000 l 7210411-5 mI 721047

Tip cone filter (pack of 50 pieces)Standard Plus

0.1-2.5 l N/A N/A0.5-10 l N/A N/A2-20 l 721008 721018

5-50 l 721008 72101810-100 l 721007 72101720-200 l 721007 72101750-200 l 721007 721017100-1000 l 721006 721016200-1000 l 721006 7210161-5 ml 721005 721015

Tip ejector collar:0.1-2.5 l 721053 (metal blue)0.5-10 l for 300 l tip 721054 (metal blue)0.5-10 l for 300 l tip 721755 (grey)0.5-10 l for 10 l tip 721053 (metal blue)0.5-10 l for 10 l tip 721754 (grey)

2-20 l 721054 (metal blue)5-50 l 721054 (metal blue)5-50 l 721700 (red)10-100 l 721054 (metal blue)20-200 l 721054 (metal blue)50-200 l 721054 (metal blue)50-200 l 721806 (yellow)100-1000 l 721055 (metal blue)200-1000 l 721055 (metal blue)200-1000 l 721080 (blue)1-5 mI 721056 (metal blue)1-5 mI 721797 (green)

Tip ejector:metal blue, 721057grey, 721753yellow, 721826red, 721704blue, 721230green, 721793

Tip ejector spring:721240

Push button:metal blue, 721059grey, 721751white, 721223yellow, 721824red, 721703blue, 721220

green, 721791

Counter:0.1-2.5 l 7211850.5-10 l 7211822-20 l 7211925-50 l 72119110-100 l 72120220-200 l 72120150-200 l 721201

100-1000 l 721212200-1000 l 7212121-5 mI 721213

N/A= Not Available


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Grip cover:metal blue, 721060grey, 721752yellow, 721825red, 721706blue, 721221

Handle, complete,in gray colour:4-channel:5-50 l 72107050-250 l 7210718-channel:0.5-10 l 7210725-50 l 72107350-300 l 72107512-channel:

0.5-10 l 7210765-50 l 72107750-300 l 721079

Piston assembly:

0.5-10 l 7212765-50 l 72127150-250 l 72127250-300 l 721278

Tip cone housing,complete, in gray colour:4-channel, 7216778-channel, 7216688-channel, 0.5-10 l 72166712-channel 72167612-channel, 0.5-10 l 721675

Tip ejector bar:4-channel 7212688-channel 7212698-channel, 0.5-10 l 72128012-channel 72127012-channel, 0.5-10 l 721666

NOTE:

Coloured spare parts presented inthese two pages are for the metal

blue/gray Biohit Proline pipettes.

Spare part codes for the whitehandles and tip cone housings are ina separate spare part list, which can

be ordered from Biohit subsidiariesor distributors.

Tip ejector, complete:metal blue, 721058grey, 721758yellow, 721862red, 721828blue, 721231

Tip ejector spring:721240

Tip cone:0.5-10 l 7212755-50 l 72127350-250 l 72127450-300 l 721277

Tip cone filter (set of 50 pcs):0.5-10 l N/A5-50 l 72101450-300 l 721014

Piston:0.5-10 l 7212965-50 l 72129750-250 l 72129850-300 l 721298

Piston spring:

0.5-10 l 7212615-50 l 72126150-250 l 72126150-300 l 721261

Spring support:

0.5-10 l 7212625-50 l 72126250-250 l 72126250-300 l 721262

Elastomer:

0.5-10 l 721300

Push button:metal blue, 721059grey, 721751yellow, 721824red, 721703blue, 721220


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Proline

Kyttohje .................................................. 1

Bruksanvisning .......................................... 17

Instruction Manual ..................................... 33

Specifications ............................................ 49


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33

CONTENTS

1. INTENDED USE ................................................34

2. YOUR NEW BIOHIT PROLINE PIPETTE ........34

2.1. Blohit Proline adjustable

volume pipettes .......................................34

2.2. Biohit Proline fixed volume pipettes .......35

2.3. Biohit Optifit tips ......................................36

3. UNPACKING .....................................................36

4. INSTALLING THE PIPETTE HOLDER .............37

5. PIPETTE MATERIALS ......................................38

6. OPERATING THE PIPETTE .............................39

6.1. Volume setting ......................................... 39

6.2. Sealing and ejecting tips .........................39

6.3. Two-in-one pipettes ................................39

6.4. Protective filters .......................................40

7. PIPETTING TECHNIQUES ...............................40

7.1. Forward pipetting .................................... 41

7.2. Reverse pipetting .................................... 41

8. STORAGE ..........................................................42

9. TESTING THE PERFORMANCE AND

RECALIBRATION ..............................................42

9.1. Testing the performance ......................... 42

9.2. Recalibration procedure ..........................44

10. MAINTENANCE ................................................44

10.1. Cleaning and decontaminating

your pipette ..............................................44

10.2. In-house maintenance ..............................45

11. TROUBLESHOOTING ......................................46

12. WARRANTY INFORMATION ...........................47

13. PERFORMANCE SPECIFICATIONS ............... 47


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1. INTENDED USE

This liquid handling device is designed and manufactured tobe used with the IVD-product as an IVD-accessory and asa stand alone laboratory equipment.

2. YOUR NEW BIOHIT PROLINE PIPETTE

Your new Biohit Proline hand-held pipette is a generalpurpose pipette, for the accurate and precise sampling

and dispensing of liquid volumes. All Biohit Proline pipettesoperate on the basis of the air displacement principle anduse disposable tips.

The range of Biohit Proline pipettes cover a volume rangefrom 0.1 l to 5 ml.

2.1. Blohit Proline adjustable volume pipettes

Single channel pipettes:

Cat.No.

Volume

Range Increment Tip

Safe-ConeFilters

50 pcs/bag

Standard Plus

720005 0.1-2.5 l 0.05 l 10 l - -

720000 0.5-10 l 0.1 l 10, 300 l - -

720080 2-20 l 0.5 l 300 l 721008 721018

720020 5-50 l 0.5 l 300, 350 l 721008 721018

720050 10-100 l 1 l 300, 350 l 721007 721017

720070 20-200 l 1 l 300, 350 l 721007 721017

720030 50-200 l 1 l 300, 350 l 721007 721017

720060 100-1000 l 5 l 1000 l 721006 721016

720040 200-1000 l 5 l 1000 l 721006 721016720110 1000-5000 l 50 l 5000 l 721005 721015


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Multichannel pipettes:

2.2. Biohit Proline fixed volume pipettes

Single channel pipettes:

Cat.No VolumeRange

Tip Safe-ConeFilters

50 pcs/bag

Standard Plus

722001 5 l 10, 300, 350 l - -

722004 10 l 10, 300, 350 l - -

722010 20 l 300, 350 l 721008 721018

722015 25 l 300, 350 l 721008 721018

722020 50 l 300, 350 l 721008 721018

722025 100 l 300, 350 l 721007 721017

722030 200 l 300, 350 l 721007 721017

722035 250 l 1000 l 721006 721016

722040 500 l 1000 l 721006 721016

722045 1000 l 1000 l 721006 721016

722050 2000 l 5000 l 721005 721015

722055 5000 l 5000 l 721005 721015

Cat.No. Volume Range Increment Tip

Safe-ConeFilters

50 pcs/bagStandard

720210 8-ch 0.5-10 l 0.1 l 10 l -

720220 8-ch 5-50 l 0.5 l 300, 350 l,350 l 4-krkin.

721014

720240 8-ch 50-300 l 5 l 350 l,350 l 4-krkin. 721014

720310 12-ch 0.5-10 l 0.1 l 10 l -

720320 12-ch 5-50 l 0.5 l 300, 350 l,350 l 4-krkin.

721014

720340 12-ch 50-300 l 5 l 350 l,

350 l 4-krkin.

721014


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2.3. Biohit Optifit tips

The full range of Biohit pipette tips are recommended for usewith Biohit Proline pipettes. Biohit standard tips are madeof virgin polypropylene. Biohit also offers a full range ofSafetySpace filter tips. Biohit standard tips are available asbulk packages, space saving refill systems and autoclavable(121C, 20 min, 1 atm) trays. Pre-sterilized tips in trays arealso available. (Fig. 1.)

Fig. 1.

3. UNPACKING

The Biohit Proline pipette package contains the followingitems:

1. Pipette2. Calibration/Opening tool3. Grease4. Instructions for use

5. Pipette holder6. Tip7. Filters (pipettes > 10 l)8. Performance certificate according to ISO 8655-6


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4. INSTALLING THE PIPETTE HOLDER

For convenience and safety always keep the pipettevertically on its own holder when not in use. When installingthe holder, please follow the instructions below:

1. Clean the shelf surface with ethanol.2. Remove the protective paper from the adhesive tape3. Install the holder as described in Figure 2A. (Make sure

the holder is pressed against the edge of the shelf.)4. Place the pipette onto the holder as shown in Figure

2B.

Note: An ergonomic, convenient carousel stand for 5 BiohitProline pipettes is also available (Cat. No.721000) (Fig. 3).

Fig. 2A

Fig. 2B

Fig. 3


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5. PIPETTE MATERIALS

Biohit Proline Single Channel

Push button rod Push button(ABS) (Operating button)

(PA)GripCover Tip ejector(PA) (PA)

Handle(ABS)

Display cover(PC)

Tip Ejectorcollar (PA)

Tip cone2.51000 l (PVDF)20005000 l (PPS)

Push button rod Push button(ABS) (PA)

Gripcover(PA) Tip Ejector

(PA)

Handle

(ABS)Display cover(PC)

Tip conehousing(ASA/PC)

Tip cone,soft sealing(PVDF)

Tip ejector (TPU)bar (PETP) (PVDF)

Hard tipcone(PC)

Biohit Proline Multichannel


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6. OPERATING THE PIPETTE

6.1. Volume setting

The volume of the pipette is clearly shown through thehandle display window. The delivery volume (variablevolume pipettes only) is set by turning the operating buttonclockwise or anticlockwise (Fig. 4). When setting the volume,please make sure that:

1. The desired delivery volume

clicks into place.2. The digits are completely visiblein the display window.

3. The selected volume is withinthe pipettes specified range.

Do not turn the operating buttonoutside the volume range because

it may jam the mechanism anddamage the pipette.

6.2. Sealing and ejecting tips

Biohit Proline tips are recommendedfor use with Biohit Proline pipettes.

Before fitting a tip ensure that thepipette tip cone is clean. Press thetip on the cone of the pipette firmlyto ensure an airtight seal. The sealis tight when a visible sealing ringforms between the tip and the blacktip cone (Fig. 5).

Each Biohit Proline pipette is fittedwith a tip ejector to help eliminatethe safety hazards associated withcontamination. The tip ejector needsto be pressed firmly downwards toensure proper tip ejection (Fig. 6).Make sure that the tip is disposedof into a suitable waste container.

6.3. Two-in-one pipettes

The Biohit Proline fixed volume pipettes 5 and 10 l, andvariable volume pipettes 0.5-10 l can be used with both 10l and 300 l tips. The pipettes are supplied with two ejectorcollars and are factory fitted with the ejector suitable for 10l tips. When using 300 l tips, please change the ejectorcollar by following the instructions below (Fig 7):

Fig. 4

Fig. 5

Fig. 6


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1. Push the tip ejector down(1.).

2. Push the opening toolpin between the ejectorbar and ejector collarto release the lockingmechanism (2.).

3. Pull the ejector collar off(3.).

4. Install the 300 l collarby pushing the collar

locking pin into the ejectorbar hole, keeping the tipejector pushed down.

5. Release the tip ejector andtest that it runs smoothly.

6.4. Protective filters

The new tip cones of BiohitProline pipettes (>10 l) allowthe use of a changeablefilter as an option. The filterprevents liquids and aerosolsfrom entering the pipette. Twotypes of filters are available: a

Safe-Cone Filter Standard anda Safe-Cone Filter Plus (seecat.no. in Chapter 2.1). Plusfilters block the passage ofliquids com-pletely. The filtersdo not affect the cali-bration ofthe pipette (Fig. 8).

Note: Change the filter on thetip cone regularly (after 50-250 pipettings).

7. PIPETTING TECHNIQUES

1. Make sure that the pipette, tips and the liquid are at the

same temperature.2. Make sure that the tip is firmly attached to the tip cone.3. Hold the pipette vertically when aspirating the liquid and

place the tip only a few millimetres into the liquid.4. Prerinse the tip before aspirating the liquid by filling and

emptying the tip 5 times. This is important especiallywhen dispensing liquids which have a viscosity anddensity different from water.

5. Always control the operating button movements with thethumb to ensure consistency.

1.

3.

2.

Fig. 7

Fig. 8


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7.1. Forward pipetting

This technique employs the blow-out function ensuringcomplete delivery of the liquid.

1. Depress the operating button to the first stop (Fig. 9B).2. Place the tip(s) just under the surface of the liquid (2-3

mm) and smoothly release the operating button up to thestarting position (Fig.9A). Carefully withdraw the tip fromthe liquid, touching against the edge of the container toremove excess.

3. Liquid is dispensed by gently depressing the operatingbutton to the first stop (Fig. 9B). After a short delaycontinue to depress the operating button to the secondstop (= blow-out) (Fig 9C). This procedure will empty thetip(s) and ensure accurate delivery.

4. Release the operating button to the starting position(Fig. 9A). If necessary change the tip(s) and continuepipetting.

7.2. Reverse pipetting

A selected volume plus an excess is aspirated into the tip. Thedelivery is done without blow-out, and so the excess volumeremains in the tip. The reverse technique is recommendedfor high viscosity, biological or foaming liquids, or very smallvolumes of liquid.

1. Depress the operating button all the way to the secondstop (Fig. 9C). Place the tip(s) just under the surface ofthe liquid (2-3 mm) and smoothly release the operatingbutton up to the starting position (Fig.9A).

2. Withdraw the tip(s) from the liquid touching against theedge of the container to remove excess.

3. Deliver the preset volume by smoothly depressing theoperating button to the first stop (Fig. 9B). Hold theoperating button at the first stop. The liquid that remainsin the tip(s) should not be included in the delivery.

4. The remaining liquid should now be discarded with thetip(s) or delivered back into the container vessel.

Starting positionFig. 9A

First stopFig. 9B

Second stopFit. 9C


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8. STORAGE

When not in use it is recommended that your Biohit Prolinepipette is stored in a vertical position. See Installing thepipette holder (Chapter 3).

9. TESTING THE PERFORMANCE ANDRECALIBRATION

It is recommended to check the performance of your

Biohit Proline pipettes regularly (e.g. every 3 months) andalways after in-house maintenance. However, the usershould establish a regular testing routine for their pipetteswith regard to accuracy requirements of the application,frequency of use, number of operators using the pipette,nature of the liquid dispensed and the acceptable maximumpermissible errors established by the user. (ISO 8655-1.)

9.1. Testing the performance

Performance testing should take place in a draught-freeroom at 15 30OC, constant to +/- 0.5OC and humidityabove 50%. The pipette, tips and the test water should havestood in the test room a sufficient time (at least 2 hours)to reach equilibrium with the room conditions. Use distilled

or deionised water (grade 3, ISO 3696). Use an analyticalbalance with a readability of 0.01 mgs. (ISO 8655-6.)

Weighing1. Adjust the desired test volume Vs.2. Carefully fit the tip onto the tip cone.3. Fill the tip with test water and expel to waste five times

to reach a humidity equilibrium in the dead air volume.

4. Replace the tip. Pre-wet the tip by filling it once with testwater and expel to waste.

5. Aspirate the test water, immersing the tip only 2-3mm below the surface of the water. Keep the pipettevertical.

6. Withdraw the pipette vertically and touch the tip againstthe inside wall of the test water container.

7. Pipette the water into the weighing vessel, touching the

tip against the inside wall of the vessel just above theliquid surface at an angle of 30 to 45. Withdraw thepipette by drawing the tip 8-10 mm along the inner wallof the weighing vessel.

8. Read the weight in mgs (mi).9. Repeat the test cycle until 10 measurements have been

recorded.10. Convert the recorded masses (m

i) to volumes (V

i)

Vi = mi * Z Z = correction factor (Table1)

11. Calculate the mean volume (v ) delivered:v= (Vi)/10


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12. For conformity evaluation calculate the systematicerror es of the measurement:in l: es = v - Vs Vs = selected testvolume

or in %: es = 100 (v - Vs)/Vs13. For conformity evaluation calculate the random error of

the measurement:

as standard deviation s = (Vi - v )2 n = number ofmeasurement (10)n - 1

or as coefficient of variation CV = 100s/v14. Compare the systematic error (inaccuracy) and random

error (imprecision) with the values in the performancespecifications (p. 48) or the specifications of your ownlaboratory. If the results fall within the specifications,the pipette is ready for use. Otherwise check bothsystematic and random errors and, when necessary,

proceed to the recalibration procedure (Chapter 9.2).

Note: Systematic error (inaccuracy) is the difference betweenthe dispensed volume and the selected test volume. Randomerror (imprecision) is the scatter of the dispensed volumesaround the mean of the dispensed volume. (ISO 8655-1.)

Note: Biohit specifications are achieved in strictly controlled

conditions (ISO 8655-6). The user should establish ownspecifications based on the field of use and the accuracyrequirements placed on the pipette (ISO8655-1).

Table 1

Z-values (l/mg):

Temp. Air Pressure (kPa)(C) 95 100 101.3 105

20.020.521.021.522.0

22.523.023.5

1.00281.00291.00301.00311.0032

1.00331.00341.0036

1.00281.00291.00311.00321.0033

1.00341.00351.0036

1.00291.00301.00311.00321.0033

1.00341.00351.0036

1.00291.00301.00311.00321.0033

1.00341.00361.0037

Note: This method is based on ISO 8655.


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9.2. Recalibration procedure

1. Place the calibrationtool into the holes of thecalibration adjustmentlock (under the operatingbutton) (Fig. 10).

2. Turn the adjustment lockanticlockwise to decreaseand clockwise to increasethe volume.

3. Repeat Performance test(Chapter 8.1.) procedurefrom step 1 until thepipetting results arecorrect.

10. MAINTENANCE

To maintain the best results from your Biohit Proline pipetteeach unit should be checked every day for cleanliness.Particular attention should be paid to the tip cone(s).

Biohit Proline pipettes have been designed for easy in-house service. However, Biohit also provides complete

repair and recalibration service including a service reportand performance certificate(s). Please return your pipetteto your local representative for repair or performance test/recalibration. Before returning please make sure that itis free from all contamination. Please advise our ServiceRepresentative of any hazardous materials which may havebeen used with your pipette.

Note: Check the performance of your Biohit Proline pipetteregularly e.g. every 3 months and always after in-houseservice or maintenance.

10.1. Cleaning and decontaminating your pipette

To clean and decontaminate your pipette, lightly spray

the outside of the pipette with Biohit Proline Biocontroldecontamination solution (Cat. No. 724004, 5 l) or useethanol. Wipe dry with a soft cloth or lint-free tissue. It isrecommended to clean and decontaminate the tip coneregularly.

Fig. 10


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10.2. In-house maintenance

1. Hold down the tip ejector.2. Place the tooth of the opening tool between the tip

ejector and the tip ejector collar to release the lockingmechanism (Fig. 11).

3. Carefully release the tip ejector and remove the tipejector collar.

4. Place the wrench end of the opening tool over the tipcone, turning it anticlockwise. Do not use any othertools (Fig. 12). The 5 ml tip cone is removed by turning it

anticlockwise without any tools (Fig. 13).5. Remove the tip cone, piston, and spring. Remove thefilter if fitted.

6. Place the tip cone, tip ejector, tip ejector collar, piston,O-ring, and spring into a beaker containing Biohit ProlineBiocontrol and leave for at least 30 minutes to securecomplete decontamination.

7. Remove the components from the beaker and rinse

them with distilled water, then dry, preferably with warmair, for at least one hour.

8. Before replacing the tip cone it is recommended togrease the piston and O-ring slightly by using thesilicone grease provided.

9. After reassembling press the operating button severaltimes to ensure that the grease has spread evenly.

10. Check the pipette calibration.

Note: Instead of Biocontrol you can use ethanol: Wipe thepiston, the O-ring, and the tip cone with ethanol and a lint-free cloth.

Note: On models 720005, 720000, 722001 and 722004 (10lpipettes) the O-ring cannot be accessed for maintenance.

Note: Excessive use of grease may jam the piston.

1.

1.

1.3.

2. 2. 2.

Fig. 11 Fig. 12 Fig. 13


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11. TROUBLESHOOTING

Trouble Possible cause Solution

Droplets leftinside the tip

Unsuitable tip

Non-uniform wettingof the tip plastic

Use original Biohit tips

Attach new tip

Laeakage orpipettedvolume

too small

Tip incorrectlyattached

Unsuitable tip

Foreign particlesbetween tip and tipcone

Instrumentcontaminated

Insufficient amountof grease on pistonand O-ring

O-ring not correctlypositioned ordamaged

Incorrect operation

Calibration alteredor unsuitable for theliquid

Instument damaged

Attach firmly

Use original Biohit tips

Clean the tip cone,attach new tip

Clean and greaseO-ring and piston,

clean the tip cone (*)

Grease accordingly

Change the O-ring

Follow instructionscarefully

Recalibrate accordingto instructions

Send for service

Push buttonjammed ormoveserratically

Piston contaminated

Penetration ofsolvent vapours

Clean and greaseO-ring and piston,clean the tip cone (*)


Pipette blocked,aspirated volumetoo small

Liquid haspenetrated tipcone and dried


Tip ejector jam-med or moveserratically

Tip cone and/orejector collarcontaminated

Clean the tip cone andthe ejector collar (*)

(*) Clean with Biohit Proline Biocontrol or 75% ethanol.


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12. WARRANTY INFORMATION

Biohit Proline pipettes are covered by warranty for 3 yearsagainst defects in materials and workmanship. Should yourProline pipette fail to function at any time, please contactyour local Biohit representative.

ANY WARRANTY WILL, HOWEVER, BE DEEMED ASVOID IF FAULT IS FOUND TO HAVE BEEN CAUSEDBY MALTREATMENT, MISUSE, UNAUTHORIZEDMAINTENANCE OF SERVICE OR NEGLIGENCE OF REGULAR

MAINTENANCE AND SERVICE, ACCIDENTAL DAMAGE,INCORRECT STORAGE OR USE OF THE PRODUCTS FOROPERATIONS OUTSIDE THEIR SPECIFIED LIMITATIONS,OUTSIDE THEIR SPECIFICATIONS, CONTRARY TO THEINSTRUCTIONS GIVEN IN THIS MANUAL OR WITH OTHERTHAN THE MANUFACTURERS ORIGINAL TIPS.

Each Biohit Proline pipette is tested before shipping by

the manufacturer. The Biohit Quality Assurance Procedureguarantees that the Biohit Proline pipette you have purchasedis ready for use.

13. PERFORMANCE SPECIFICATIONS

The manufacturer's specifications (p. 48) are guaranteed

only when the manufacturer's original tips are used. Themanufacturer's specifications should be used as guidelineswhen establishing your own performance specifications inaccordance with ISO 8655.


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Specifications

Biohit Proline Single Channel Adjustable VolumePipettes

Volume Inaccuracy ImprecisionCat.No. range Volume

720005 0.1-2.5 l 2.5 l 2.50% 2.00%1.25 l 3.00% 3.00%

0.25 I 12.00% 6.00%720000 0.5-10 l 10 l 1.00% 0.80%

5 l 1.50% 1.50%1 l 2.50% 1.50%

700080 2-20 l 20 l 0.90% 0.40%10 l 1.20% 1.00%2 l 3.00% 2.00%

720020 5-50 l 50 l 0.60% 0.30%25 l 0.90% 0.60%5 l 2.00% 2.00%

700050 10-100 l 100 l 0.80% 0.20%50 l 1.00% 0.40%10 l 3.00% 1.00%

720070 20-200 l 200 l 0.60% 0.20%100 l 0.80% 0.30%20 l 2.50% 0.80%

720060 100-1000 l 1000 l 0.60% 0.20%500 l 0.70% 0.25%100 l 2.00% 0.70%

720110 1-5 ml 5 ml 0.50% 0.20%2.5 ml 0.60% 0.30%

1 ml 0.70% 0.30%


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Inaccuracy ImprecisionCat.No. Volume range Volume

720210 8-ch 0.5-10 l 10 l 1.50% 1.50%5 l 2.50% 2.50%1 l 4.00% 4.00%

720220 8-ch 5-50 l 50 l 1.00% 0.50%25 l 1.50% 1.00%5 l 3.00% 2.00%

720240 8-ch 50-300 l 300 l 0.70% 0.25%150 l 1.00% 0.50%50 l 1.50% 0.80%

720310 12-ch 0.5-10 l 10 l 1.50% 1.50,%5 l 2.50% 2.50%1 l 4.00% 4.00%

720320 12-ch 5-50 l 50 l 1.00% 0.50%

25 l 1.50% 1.00%5 l 3.00% 2.00%

720340 12-ch 50-300 l 300 l 0.70% 0.25%150 l 1.00% 0.50%50 l 1.50% 0.80%

Biohit Proline Fixed Volume Pipettes

Inaccuracy ImprecisionCat.No. Volume range Volume

722001 5 l 5 l 1.30% 1.20%

722004 10 l 10 l 0.80% 0.80%

722010 20 l 20 l 0.60% 0.50%

722015 25 l 25 l 0.50% 0.30%

722020 50 l 50 l 0.50% 0.30%

722025 100 l 100 l 0.50% 0.30%

722030 200 l 200 l 0.40% 0.20%

722035 250 l 250 l 0.40% 0.20%722040 500 l 500 l 0.30% 0.20%

722045 1000 l 1000 l 0.30% 0.20%

722050 2000 l 2000 l 0.30% 0.15%

722055 5000 l 5000 l 0.30% 0.15%

Liquid: Distilled water (grade 3, ISO 3696)

Reference temperature: 22C. constant to 0.5C

Tested: According to ISO 8655 using originalBiohit tips

Biohit Proline Multichannel Adjustable Volume Pipettes


22/22

Proline

Kyttohje

Bruksanvisning

Instruction Manual

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Proline Mechanical Eng

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