Protection of Penaeus monodon against white spot syndrome.pptx

7/29/2019 Protection of Penaeus monodon against white spot syndrome.pptx

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IntroductionWhite spot syndrome

Caused by white spot syndrome virus (WSSV)

Lethal and contagious disease of Penaeid shrimps First reported in Taiwan; 1992

Outbreaks led to collapse of shrimp aquacultureindustry

Disease found in the Philippines in 2000 (Magbanua,2000)


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Prevention Broodstock genetic selection

Water treatment

Vaccination Clean, wild and genetically diverse brood stock

Disinfectants

Application of yeast and Chlorella in treating the water


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Methods Treatments

Tank with Chlorella culture

Tank with greenwater culture stocked with tilapia Tank added with molasses

Tank with seawater


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Results


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Results


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Results


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Results


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Results


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Results


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Summary Tanks with greenwater has the least WSS infection

Howevere tanks with greenwater has the least survival;

attributed to ammonia build-up caused by the tilapia Greenwater with molasses is recommended for

prevention of white spot syndrome. Molasses couldreduce NH3 in the water.


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Methods Oral vaccination through feeding of vaccine-coated

pellets

Treatments V19 coated food pellets

V28 coated food pellets

pET28a coated food pellet

Possitive control Negative control


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Time-mortality relationship of vaccination experiment 1. Cumulative mortality rates of shrimp from

the experimental groups vaccinated with VP19 (), VP28 (), VP19 plus VP28 (*), pET plus pMAL (),

positive control (), and negative control () as indicated in Table Table11 are plotted against the

time after challenge. Lines marked with an asterisk are significantly different from the pET plus

pMAL and positive control groups.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC369486/table/t1/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC369486/table/t1/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC369486/table/t1/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC369486/table/t1/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC369486/table/t1/


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Time-mortality relationship of

vaccination experiment 2.

Shrimp were challenged 3 days(a), 7 days (b), and 21 days (c)

after cessation of feeding coated

food pellets. Cumulative

mortality rates of shrimp from

the experimental groups

vaccinated with VP28 (), pET (),positive control (), and

negative control () as

indicated in Table Table11 are

plotted against the time after

challenge. Lines marked with an

asterisk are significantlydifferent from the pET and

positive control groups.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC369486/table/t1/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC369486/table/t1/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC369486/table/t1/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC369486/table/t1/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC369486/table/t1/


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MethodsYeast (C. awuaetestoris)culture are introduce at three

different intervals

G1 (Daily treatment)

G3 (Every three days)

G5 (Every 5 days)

G7 (Every 7 days)

G10 (Every 10 days) C (Control)

After 30 days, WSSV is introduced and the yeasttreatment were withdrawn/stopped.


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Fig. 1. Mean (SD) post challenge survival in P. monodon post larvae when fed with 10% marine

yeast C. aquaetextoris S527 at different frequencies and challenged with WSSV. *Data at the same

exposure time with different letters are significantly different (P < 0.05). G1 = Daily, G3 = three days

interval, G5 = five days interval, G7 = seven days interval, G10 = ten days interval.


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Summary Results showed that shrimp treated with yeast culture

every 7 days have the highest post challenge survival.

Yeast (Candida aquetextoris)culture has the potentialto become a immunostimulant and improve immuneresponse ofPenaeus monodon against WSSV


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Summary and Conclusion Introduction of greenwater and yeast (C. aquatextoris)

into the tiger prawn (P. monodon) tanks/cages couldincrease their survival in a white spot syndrome

outbreak.

Vaccination with viral proteins (specifically VP28)could improve immunity of P. monodonWSSV whileintroduction of pET28a increases mortality.


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ReferencesWitteveldt, Cifuentes, Vlak & van Hulten. 2004. Protection ofPenaeus monodon against White Spot Syndrome Virus by Oral

Vaccination. J. Virol. February 2004 vol. 78 no. 4 2057-2061Babu, Antony, Joseph, Bright & Philip. 2013. Marine yeast Candida

aquaetextoris S527 as a potential immunostimulantin black tigershrimp Penaeus monodon. Journal of Invertebrate Pathology 112(2013) 243252

Tendencia, Bosma, & Sorio. 2012. Effect of three innovative culturesystems on water quality and whitespot syndrome virus (WSSV)

viral load in WSSV-fed Penaeus monodon cultured in indoortanks. Aquaculture 350-353 (2012) 169

174

McClennen. 2004. White spot syndrome virus: the economic,environmental and technical implications on the developmentof latin american shrimp farming. Retrieved fromdl.tufts.edu/file_assets/tufts:UA015.012.DO.00040

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