Date post: | 27-Nov-2014 |
Category: |
Science |
Upload: | azmansamad2 |
View: | 430 times |
Download: | 2 times |
INTRODUCTION
Introduction
Solution to sexual incompatibility barriers – distant species;
Fusion of cells – full hybrids – crop improvement programmes;
EC Cocking (1960) – enzymatic degradation of plant cell wall - viable naked cells
Freshly isolated mesophyll protoplasts
JB Power, UK
Introduction
ISOLATION OF PROTOPLASTS
ISOLATION OF PROTOPLASTS FROM PLANTLETS OF Solanum phureja
Freshly isolated
First mitotic division
Multicellular colonies (7-d-old)
Macroscopic p-calli (several wks)
Factors that influence yield and viability of protoplasts
Factors that influence yield and viability of protoplasts
Factors that influence yield and viability of protoplasts
Factors that influence yield and viability of protoplasts
Commercial Enzymes
Factors that influence yield and viability of protoplasts
Factors that influence yield and viability of protoplasts
Factors that influence yield and viability of protoplasts
Viability of the Protoplasts
Detection methods:
1)Observation of cyclosis or cytoplasmic streaming;
2)Oxygen uptake
3)Photosynthetic activity;
4)Staining with fluroescein diacetate - common
Nutritional Requirements
Plant regeneration
- Plant regeneration from hypocotyl protoplasts of Brassica napus
Stage
Event
AFreshly isolated
protoplasts
B 1-d-old protoplasts
C Callus (14-d culture)
D Shoot buds differentiated
ECalli with regenerated shoots (Zeatin + IAA)
F Plant regenerated