36
© F e r m e n t i s 2 0 1 5 – A l l r i g h t s r e s e r v e d
Gx6000
Yeast Shaping
Membrane integrity, stable and ready to assimilate sugars
Active dry yeast
Drying
Full size Size reduction Folding
→
→
→
→
20 min
°
°
Concentration Rehydrate the dried yeasts in 10 times their weight in the medium* (water or other)
Time 45 min
Agitation 1. Without agitation (WA) : the yeast is put on liquid surface, rehydration is realized tranquilly without agitation.
2. Moderated agitation (MA) : the yeast is put on liquid surface, rehydration is realized tranquilly without agitation during 15' and after agitation is maintained at 100 rpm.
3. Violent agitation (VA) : the yeast is put in a sterile flask, the medium is poured on the yeast and a violent agitation is realized every 2 or 3 minutes
Temperature °C 8, 12, 16, 20, 32, 40°C (46, 53, 61, 68, 90, 104oF)
*Medium Distilled waterMineral waterTap water7% ethanolWort at 7°PWort at 15°PWort at 25°P
→ Beckman Coulter Vi-CELLthat automates the widely accepted trypan blue cell viability
Measurement
50,0
60,0
70,0
80,0
90,0
100,0
8 12 16 20 32 40Temperature (°C)
T-58 strain
50,0
60,0
70,0
80,0
90,0
100,0
8 12 16 20 32 40Temperature (°C)
US-05 strain
50,0
60,0
70,0
80,0
90,0
100,0
8 12 16 20 32 40Temperature (°C)
S-33 strain
°
>50%
50,0
60,0
70,0
80,0
90,0
100,0
8 12 16 20 32 40Temperature (°C)
S-23 strain
50,0
60,0
70,0
80,0
90,0
100,0
8 12 16 20 32 40Temperature (°C)
S-189 strain
●
●
●
60,0
65,0
70,0
75,0
80,0
85,0
90,0
95,0
100,0
T58 US05 S33 BE256 S04 K97 F2 WB06
Via
bili
ty (
%)
Ales
60,0
65,0
70,0
75,0
80,0
85,0
90,0
95,0
100,0
S23 S189 W34/70
Via
bili
ty (
%)
8°C
12°C
16°C
20°C
32°C
40°C
Lagers
60,00
65,00
70,00
75,00
80,00
85,00
90,00
95,00
100,00
15 25 35 45
Via
bili
ty (
%)
Time of rehydration (min)
T58
US05
S33
60,00
65,00
70,00
75,00
80,00
85,00
90,00
95,00
100,00
15 25 35 45
Via
bili
ty (
%)
Time of rehydration (min)
S23
S189
Ales Lagers
At moderate agitation and 32°C
50,0
55,0
60,0
65,0
70,0
75,0
80,0
85,0
90,0
95,0
100,0
T58 S23 US05
Via
bili
ty (
%)
Distilled
Mineral
Tap
At moderate agitation and 32°C
● Water quality does not influence significantly viability during rehydration of dried yeast● Viability varies from 75% to maximum 95% all conditions combined
●
●
60,00
65,00
70,00
75,00
80,00
85,00
90,00
95,00
100,00
T58 S23 US05
Via
bili
ty (
%)
20°C
Water
Wort 7°P
Wort 15°P
Wort 25°P
60,00
65,00
70,00
75,00
80,00
85,00
90,00
95,00
100,00
T58 S23 US05V
iab
ility
(%
)
7°C
Water
Wort 7°P
Wort 15°P
Wort 25°P
At moderate agitation
Ale strains Lager strains
Strains
Rehydration conditions
Pitching rate Standard wortTemperature
SafAle : US-05, S-33 and T-58
- In water at 30°C with moderate agitation (MA): W- In 15°P wort at 30°C with MA: 15°P- No rehydration → Direct pitch: DP
50 g/hl 15°P20°C
SafLager : S-23, W-34/70
- In water at 30°C with moderate agitation (MA): W- In 15°P wort at 30°C with MA: 15°P- No rehydration → Direct pitch: DP
100 g/hl15°P14°C
Tests of fermentation performances in triplicate: - Evaluation by measuring the decrease of weight of the medium- Correlation to the sugar conversion into CO2 and ethanol.
Analyses realized at the end of fermentation by HPLC:- Ethanol production- volatile compounds
- acetaldehyde- esters- higher alcohols- vicinal diketones
0,00
1,00
2,00
3,00
4,00
5,00
6,00
0,00 2,00 4,00 6,00 8,00
Eth
ano
l (%
v/v
)
Time (day)
S33_DP
S33_W
S33_15°P
5,00
5,20
5,40
5,60
5,80
6,00
S33_DP S33_W S33_15°P
Eth
ano
l (%
v/v
)
65,00
67,00
69,00
71,00
73,00
75,00
S33_DP S33_W S33_15°P
AD
F (%
)
0,00
50,00
100,00
150,00
200,00
250,00
S33_DP S33_W S33_15°P
Vo
lati
le c
om
po
un
ds
VDK (ppb)
Higher alcohols (ppm)
Esters (ppm)
Acetaldehyde (ppm)
0,00
1,00
2,00
3,00
4,00
5,00
6,00
7,00
0,00 2,00 4,00 6,00 8,00
Eth
ano
l (%
v/v
)
Time (day)
T58_DP
T58_W
T58_15°P
6,00
6,10
6,20
6,30
6,40
6,50
T58_DP T58_W T58_15°P
Eth
ano
l (%
v/v
)
75,00
77,00
79,00
81,00
83,00
85,00
T58_DP T58_W T58_15°PA
DF
(%)
0,00
50,00
100,00
150,00
200,00
250,00
300,00
T58_DP T58_W T58_15°P
Vo
lati
le c
om
po
un
ds
VDK (ppb)
Higher alcohols (ppm)
Esters (ppm)
Acetaldehyde (ppm)
0,00
1,00
2,00
3,00
4,00
5,00
6,00
7,00
0,00 2,00 4,00 6,00 8,00
Eth
ano
l (%
v/v
)
Time (day)
US05_DP
US05_W
US05_15°P
6,00
6,20
6,40
6,60
6,80
7,00
US05_DP US05_W US05_15°P
Eth
ano
l (%
v/v
)75,00
77,00
79,00
81,00
83,00
85,00
US05_DP US05_W US05_15°PA
DF
(%)
0,00
50,00
100,00
150,00
200,00
250,00
300,00
350,00
US05_DP US05_W US05_15°P
Vo
lati
le c
om
po
un
ds
VDK (ppb)
Higher alcohols (ppm)
Esters (ppm)
Acetaldehyde (ppm)
0,00
1,00
2,00
3,00
4,00
5,00
6,00
7,00
8,00
0,00 5,00 10,00 15,00
Eth
ano
l (%
v/v
)
Time (day)
W34/70_DP
W34/70_W
W34/70_15°P
5,00
5,50
6,00
6,50
7,00
7,50
W34/70_DP W34/70_W W34/70_15°P
Eth
ano
l (%
v/v
)65,00
70,00
75,00
80,00
85,00
90,00
95,00
W34/70_DP W34/70_W W34/70_15°P
AD
F (%
)
0,00
50,00
100,00
150,00
200,00
250,00
300,00
350,00
400,00
450,00
W34/70_DP W34/70_W W34/70_15°P
Vo
lati
le c
om
po
un
ds
VDK (ppb)
Higher alcohols (ppm)
Esters (ppm)
Acetaldehyde (ppm)
0,00
1,00
2,00
3,00
4,00
5,00
6,00
7,00
8,00
0,00 2,00 4,00 6,00 8,00 10,00 12,00
Eth
ano
l (%
v/v
)
Time (day)
S23_DP
S23_W
S23_15°P
6,00
6,20
6,40
6,60
6,80
7,00
S23_DP S23_W S23_15°P
Eth
ano
l (%
v/v
)78,00
79,00
80,00
81,00
82,00
83,00
84,00
S23_DP S23_W S23_15°PA
DF
(%)
0,00
100,00
200,00
300,00
400,00
500,00
600,00
S23_DP S23_W S23_15°P
Vo
lati
le c
om
po
un
ds
VDK (ppb)
Higher alcohols (ppm)
Esters (ppm)
Acetaldehyde (ppm)
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