*Quanti-Tray and Colilert are trademarks or registered trademarks of IDEXX Laboratories, Inc. or its affiliates in the United States and/or other countries.
The method Colilert*-18/Quanti-Tray*/2000 for water analysis is granted NF Validation by AFNOR Certification as an alternative method to the standard ISO 9308-3 for detection and enumeration of Escherichia coli ß-glucuronidase positive in bathing water, under the Certificate number: IDX 33/02 – 06/12 For more information about end of validity, please refer to the certificate NF Validation available on website mentioned above
La méthode Colilert*-18 / Quanti-Tray*/2000 pour le contrôle des eaux est certifiée NF Validation par AFNOR Certification comme méthode alternative à la norme NF EN ISO 9308-3 pour le dénombrement des Escherichia coli ß-glucuronidase positive dans les eaux de baignades sous le n° d’attestation: IDX 33/02 – 06/12. La date de fin de validité de la certification NF Validation est précisée sur l’attestation, disponible auprès d’IDEXX ou d’AFNOR Certification.
IDX 33/02 – 06/12WATER ANALYSIS METHODS
www.afnor-validation.org
Insert and Most Probable Number (MPN) Table
Quanti-Tray* Certificate of SterilityThis certifies that the enclosed Quanti-Tray/2000 trays have been sterilized with ethylene oxide.
IntroductionIDEXX Quanti-Tray*/2000 is designed to give quantitated bacterial counts of 100 mL samples using IDEXX reagent products. Add the reagent/sample mixture to a Quanti-Tray/2000, seal it in a Quanti-Tray* Sealer and incubate per the reagent instructions. Count the number of positive large and small wells and use the Most Probable Number (MPN) Table attached to determine the MPN.
Quanti-Tray* upright with the well side facing the palm.
2. Squeeze the upper part of the Quanti-Tray so that the Quanti-Tray bends toward the palm.
3. Gently pull foil tab to separate the foil from the tray. Avoid touching the inside of the foil or tray.
4. Pour the reagent/sample mixture directly into the Quanti-Tray, avoiding contact with the foil tab. Tap the small wells 2–3 times to release any air bubbles. Allow foam to settle.
5. Place the sample-filled Quanti-Tray onto the Quanti-Tray/2000 rubber insert of the Quanti-Tray Sealer with the well side (plastic) of the Quanti-Tray facing down.
6. Seal according to the Quanti-Tray Sealer instructions.
7. Incubate according to reagent instructions.
8. Count large and small positive wells and refer to the Quanti-Tray/2000 MPN table to find the MPN.†
9. Dispose of media in accordance with good laboratory practices.
English
† Download the IDEXX MPN generator software for automated Quanti-Tray results at idexx.com/mpngenerator.
* Quanti-Tray is a trademark or registered trademark of IDEXX Laboratories, Inc. in the United States and/or other countries.
† Téléchargez le logiciel de détermination du NPP d’IDEXX à l’adresse idexx.com/mpngenerator pour obtenir des résultats automatisés avec le dispositif Quanti-Tray.
*Quanti-Tray est une marque de fabrique ou une marques déposée d'IDEXX Laboratories, Inc. ou ses filiales aux États-Unis et/ou dans d'autres pays.
IntroductionLes plateaux Quanti-Tray*/2000 d’IDEXX, spécialement conçus pour effectuer des numérations bactériennes sur des échantillons de 100 millilitres, font appel à la technologie d’IDEXX lorqu’ utilisés avec les réactifs IDEXX. Versez le mélange réactif/échantillon dans un Quanti-Tray/2000, placez ensuite le tout dans le Quanti-Tray* Sealer et procédez à l’incubation du mélange selon les instructions accompagnant les réactifs. Comptez ensuite le nombre de cupules positives et référez-vous à la table ci-dessous pour déterminer le nombre le plus probable (NPP).
ContetuCette boîte contient des Quanti-Tray/2000 stériles.
Mode d'emploi1. Prenez un Quanti-Tray* et tenez-le à
la verticale en veillant à orienter les cupules vers la paume de la main.
2. Appuyez sur la partie supérieure du Quanti-Tray afin qu’il vienne se loger dans la paume de la main.
3. Ouvrez le Quanti-Tray en retirant la feuille en aluminium. Evitez tout contact avec la partie intérieure de l’aluminium ou du plateau.
4. Versez directement le mélange réactif/échantillon dans le Quanti-Tray en évitant tout contact avec la languette d’aluminium. Tapoter les petits puits 2 à 3 fois afin de libérer les bulles d'air. Laisser la mousse se dissiper.
5. Placez le Quanti-Tray rempli d’échantillons sur le porte-plateau en caoutchouc du Quanti-Tray Sealer en veillant à orienter les cupules (parties en plastique) vers le bas afin qu’elles viennent s’encastrer parfaitement dans le porte-plateau.
6. Scellez le Quanti-Tray conformément aux instructions accompagnant le Sealer.
7. Incubez conformément aux instructions accompagnant le réactif.
8. Comptez le nombre de cupules positives, puis référez-vous à la table NPP au verso pour déterminer le nombre le plus probable (NPP).†
9. Débarrassez-vous du milieu en suivant les bonnes pratiques de laboratoire.
Française
Pour obtenir l’assistance technique, veuillez appeler :
Europe: +00800 4339 9111
idexx.fr/eau
Tab
le N
PP
IDE
XX
Qu
anti
-Tra
y* /200
0#
Gra
nd
s P
uit
s P
osi
tifs
# P
etit
s P
uit
s P
osi
tifs
01
23
45
67
89
1011
1213
1415
1617
1819
2021
2223
24
0<
11.
02.
03.
04.
05.
06.
07.
08.
09.
010
.011
.012
.013
.014
.115
.116
.117
.118
.119
.120
.221
.222
.223
.324
.3
11.
02.
03.
04.
05.
06.
07.
18.
19.
110
.111
.112
.113
.214
.215
.216
.217
.318
.319
.320
.421
.422
.423
.524
.525
.6
22.
03.
04.
15.
16.
17.
18.
19.
210
.211
.212
.213
.314
.315
.416
.417
.418
.519
.520
.621
.622
.723
.724
.825
.826
.9
33.
14.
15.
16.
17.
28.
29.
210
.311
.312
.413
.414
.515
.516
.517
.618
.619
.720
.821
.822
.923
.925
.026
.127
.128
.2
44.
15.
26.
27.
28.
39.
310
.411
.412
.513
.514
.615
.616
.717
.818
.819
.921
.022
.023
.124
.225
.326
.327
.428
.529
.6
55.
26.
37.
38.
49.
410
.511
.512
.613
.714
.715
.816
.917
.919
.020
.121
.222
.223
.324
.425
.526
.627
.728
.829
.931
.0
66.
37.
48.
49.
510
.611
.612
.713
.814
.916
.017
.018
.119
.220
.321
.422
.523
.624
.725
.826
.928
.029
.130
.231
.332
.4
77.
58.
59.
610
.711
.812
.813
.915
.016
.117
.218
.319
.420
.521
.622
.723
.824
.926
.027
.128
.329
.430
.531
.632
.833
.9
88.
69.
710
.811
.913
.014
.115
.216
.317
.418
.519
.620
.721
.822
.924
.125
.226
.327
.428
.629
.730
.832
.033
.134
.335
.4
99.
810
.912
.013
.114
.215
.316
.417
.618
.719
.820
.922
.023
.224
.325
.426
.627
.728
.930
.031
.232
.333
.534
.635
.837
.0
1011
.012
.113
.214
.415
.516
.617
.718
.920
.021
.122
.323
.424
.625
.726
.928
.029
.230
.331
.532
.733
.835
.036
.237
.438
.6
1112
.213
.414
.515
.616
.817
.919
.120
.221
.422
.523
.724
.826
.027
.228
.329
.530
.731
.933
.034
.235
.436
.637
.839
.040
.2
1213
.514
.615
.816
.918
.119
.320
.421
.622
.823
.925
.126
.327
.528
.629
.831
.032
.233
.434
.635
.837
.038
.239
.540
.741
.9
1314
.816
.017
.118
.319
.520
.621
.823
.024
.225
.426
.627
.829
.030
.231
.432
.633
.835
.036
.237
.538
.739
.941
.242
.443
.6
1416
.117
.318
.519
.720
.922
.123
.324
.525
.726
.928
.129
.330
.531
.733
.034
.235
.436
.737
.939
.140
.441
.642
.944
.245
.4
1517
.518
.719
.921
.122
.323
.524
.725
.927
.228
.429
.630
.932
.133
.334
.635
.837
.138
.439
.640
.942
.243
.444
.746
.047
.3
1618
.920
.121
.322
.623
.825
.026
.227
.528
.730
.031
.232
.533
.735
.036
.337
.538
.840
.141
.442
.744
.045
.346
.647
.949
.2
1720
.321
.622
.824
.125
.326
.627
.829
.130
.331
.632
.934
.135
.436
.738
.039
.340
.641
.943
.244
.545
.947
.248
.549
.851
.2
1821
.823
.124
.325
.626
.928
.129
.430
.732
.033
.334
.635
.937
.238
.539
.841
.142
.443
.845
.146
.547
.849
.250
.551
.953
.2
1923
.324
.625
.927
.228
.529
.831
.132
.433
.735
.036
.337
.639
.040
.341
.643
.044
.345
.747
.148
.449
.851
.252
.654
.055
.4
2024
.926
.227
.528
.830
.131
.532
.834
.135
.436
.838
.139
.540
.842
.243
.644
.946
.347
.749
.150
.551
.953
.354
.756
.157
.6
2126
.527
.929
.230
.531
.833
.234
.535
.937
.338
.640
.041
.442
.844
.145
.546
.948
.449
.851
.252
.654
.155
.556
.958
.459
.9
2228
.229
.530
.932
.333
.635
.036
.437
.739
.140
.541
.943
.344
.846
.247
.649
.050
.551
.953
.454
.856
.357
.859
.360
.862
.3
2329
.931
.332
.734
.135
.536
.838
.339
.741
.142
.543
.945
.446
.848
.349
.751
.252
.754
.255
.657
.158
.660
.261
.763
.264
.7
2431
.733
.134
.535
.937
.338
.840
.241
.743
.144
.646
.047
.549
.050
.552
.053
.555
.056
.558
.059
.561
.162
.664
.265
.867
.3
2533
.635
.036
.437
.939
.340
.842
.243
.745
.246
.748
.249
.751
.252
.754
.355
.857
.358
.960
.562
.063
.665
.266
.868
.470
.0
2635
.536
.938
.439
.941
.442
.844
.345
.947
.448
.950
.452
.053
.555
.156
.758
.259
.861
.463
.064
.766
.367
.969
.671
.272
.9
2737
.438
.940
.442
.043
.545
.046
.548
.149
.651
.252
.854
.456
.057
.659
.260
.862
.464
.165
.767
.469
.170
.872
.574
.275
.9
2839
.541
.042
.644
.145
.747
.348
.850
.452
.053
.655
.256
.958
.560
.261
.863
.565
.266
.968
.670
.372
.073
.775
.577
.379
.0
2941
.743
.244
.846
.448
.049
.651
.252
.854
.556
.157
.859
.561
.262
.964
.666
.368
.069
.871
.573
.375
.176
.978
.780
.582
.4
3043
.945
.547
.148
.750
.452
.053
.755
.457
.158
.860
.562
.264
.065
.767
.569
.371
.072
.974
.776
.578
.380
.282
.184
.085
.9
3146
.247
.949
.551
.252
.954
.656
.358
.159
.861
.663
.365
.166
.968
.770
.572
.474
.276
.178
.079
.981
.883
.785
.787
.689
.6
3248
.750
.452
.153
.855
.657
.359
.160
.962
.764
.566
.368
.270
.071
.973
.875
.777
.679
.581
.583
.585
.487
.589
.591
.593
.6
3351
.253
.054
.856
.558
.360
.262
.063
.865
.767
.669
.571
.473
.375
.277
.279
.281
.283
.285
.287
.389
.391
.493
.695
.797
.8
3453
.955
.757
.659
.461
.363
.165
.067
.068
.970
.872
.874
.876
.878
.880
.882
.985
.087
.189
.291
.493
.595
.797
.910
0.2
102.
4
3556
.858
.660
.562
.464
.466
.368
.370
.372
.374
.376
.378
.480
.582
.684
.786
.989
.191
.393
.595
.798
.010
0.3
102.
610
5.0
107.
3
3659
.861
.763
.765
.767
.769
.771
.773
.875
.978
.080
.182
.384
.586
.788
.991
.293
.595
.898
.110
0.5
102.
910
5.3
107.
711
0.2
112.
7
3762
.965
.067
.069
.171
.273
.375
.477
.679
.882
.084
.286
.588
.891
.193
.495
.898
.210
0.6
103.
110
5.6
108.
111
0.7
113.
311
5.9
118.
6
3866
.368
.470
.672
.774
.977
.179
.481
.683
.986
.288
.691
.093
.495
.898
.310
0.8
103.
410
5.9
108.
611
1.2
113.
911
6.6
119.
412
2.2
125.
0
3970
.072
.274
.476
.778
.981
.383
.686
.088
.490
.993
.495
.998
.410
1.0
103.
610
6.3
109.
011
1.8
114.
611
7.4
120.
312
3.2
126.
112
9.2
132.
2
4073
.876
.278
.580
.983
.385
.788
.290
.893
.395
.998
.510
1.2
103.
910
6.7
109.
511
2.4
115.
311
8.2
121.
212
4.3
127.
413
0.5
133.
713
7.0
140.
3
4178
.080
.583
.085
.588
.090
.693
.395
.998
.710
1.4
104.
310
7.1
110.
011
3.0
116.
011
9.1
122.
212
5.4
128.
713
2.0
135.
413
8.8
142.
314
5.9
149.
5
4282
.685
.287
.890
.593
.296
.098
.810
1.7
104.
610
7.6
110.
611
3.7
116.
912
0.1
123.
412
6.7
130.
113
3.6
137.
214
0.8
144.
514
8.3
152.
215
6.1
160.
2
4387
.690
.493
.296
.099
.010
1.9
105.
010
8.1
111.
211
4.5
117.
812
1.1
124.
612
8.1
131.
713
5.4
139.
114
3.0
147.
015
1.0
155.
215
9.4
163.
816
8.2
172.
8
4493
.196
.199
.110
2.2
105.
410
8.6
111.
911
5.3
118.
712
2.3
125.
912
9.6
133.
413
7.4
141.
414
5.5
149.
715
4.1
158.
516
3.1
167.
917
2.7
177.
718
2.9
188.
2
4599
.310
2.5
105.
810
9.2
112.
611
6.2
119.
812
3.6
127.
413
1.4
135.
413
9.6
143.
914
8.3
152.
915
7.6
162.
416
7.4
172.
617
8.0
183.
518
9.2
195.
120
1.2
207.
5
4610
6.3
109.
811
3.4
117.
212
1.0
125.
012
9.1
133.
313
7.6
142.
114
6.7
151.
515
6.5
161.
616
7.0
172.
517
8.2
184.
219
0.4
196.
820
3.5
210.
521
7.8
225.
423
3.3
4711
4.3
118.
312
2.4
126.
613
0.9
135.
414
0.1
145.
015
0.0
155.
316
0.7
166.
417
2.3
178.
518
5.0
191.
819
8.9
206.
421
4.2
222.
423
1.0
240.
024
9.5
259.
527
0.0
4812
3.9
128.
413
3.1
137.
914
3.0
148.
315
3.9
159.
716
5.8
172.
217
8.9
186.
019
3.5
201.
420
9.8
218.
722
8.2
238.
224
8.9
260.
327
2.3
285.
129
8.7
313.
032
8.2
4913
5.5
140.
814
6.4
152.
315
8.5
165.
017
2.0
179.
318
7.2
195.
620
4.6
214.
322
4.7
235.
924
8.1
261.
327
5.5
290.
930
7.6
325.
534
4.8
365.
438
7.3
410.
643
5.2
Tab
le N
PP
IDE
XX
Qu
anti
-Tra
y* /200
0#
Gra
nd
s P
uits
P
osi
tifs
# P
etit
s P
uit
s P
osi
tifs
2526
2728
293
031
323
33
43
53
637
38
394
041
424
34
44
54
647
48
025
.326
.427
.428
.429
.530
.531
.532
.633
.634
.735
.736
.837
.838
.940
.041
.042
.143
.144
.245
.346
.347
.448
.549
.5
126
.627
.728
.729
.830
.831
.932
.934
.035
.036
.137
.238
.239
.340
.441
.442
.543
.644
.745
.746
.847
.949
.050
.151
.2
227
.929
.030
.031
.132
.233
.234
.335
.436
.537
.538
.639
.740
.841
.943
.044
.045
.146
.247
.348
.449
.550
.651
.752
.8
329
.330
.431
.432
.533
.634
.735
.836
.837
.939
.040
.141
.242
.343
.444
.545
.646
.747
.848
.950
.051
.252
.353
.454
.5
430
.731
.832
.833
.935
.036
.137
.238
.339
.440
.541
.642
.843
.945
.046
.147
.248
.349
.550
.651
.752
.954
.055
.156
.3
532
.133
.234
.335
.436
.537
.638
.739
.941
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510
4.3
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110
7.9
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310
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1.9
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0.7
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7.3
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1.4
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1.5
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0.8
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8.5
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0.7
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9.2
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4.4
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8.9
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3.5
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7.8
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7.8
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3.7
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8.9
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312
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914
4.6
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0.8
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6.4
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32.9
1986
.324
19.6
>24
19.6
One IDEXX DriveWestbrook, Maine 04092 USA
Quanti-Tray*/2000
†Scarica il software IDEXX per determinare il MPN dal sito idexx.com/mpngenerator per ottenere risultati automatizzati con Quanti-Tray.
*Quanti-Tray è un marchio di proprietà di, e/o registrato da, IDEXX Laboratories, Inc. o di suoi associate e protetto negli Stati Uniti e/o in altri paesi.
IntroduzioneQuanti-Tray*/2000 IDEXX sono concepiti per fornire la densità batterica (quantitativa) di campioni da 100 ml utilizzando reagenti IDEXX. Aggiungere la miscela di reagente/campione ad un Quanti-Tray/2000, inserire quest’ultimo nel Quanti-Tray* Sealer ed incubare in conformità alle istruzioni fornite con il reagente. Contare quindi il numero di pozzetti positivi e, con l’ausilio della tabella MPN allegata, determinare il numero più probabile (MPN).
Istruzioni per l’uso1. Con una mano, tenere il Quanti-Tray*
in posizione verticale, con il lato dei pozzetti rivolto verso il palmo.
2. Comprimere la parte superiore del Quanti-Tray in modo da fletterlo verso il palmo.
3. Aprire il Quanti-Tray tirando la linguetta in alluminio in direzione opposta al lato dei pozzetti. Evitare di toccare la parte interna del foglio protettivo in alluminio o del dispositivo a pozzetti.
4. Versare direttamente la miscela di reagente/campione nel Quanti-Tray, evitando di toccare la linguetta in alluminio. Dare 2–3 leggeri colpi ai pozzetti piccoli per eliminare eventuali bolle d'aria. Consentire alla schiuma di sedimentare.
5. Una volta versata la miscela, collocare il Quanti-Tray nel portapozzetti in gomma del Quanti-Tray Sealer, con il lato dei pozzetti (in plastica) rivolto verso il basso, in modo da assicurarne il corretto posizionamento.
6. Sigillare secondo le istruzioni del Quanti-Tray Sealer.
7. Incubare secondo le istruzioni fornite con il reagente.
8. Contare i pozzetti positivi e consultare la tabella MPN sul retro del foglio delle istruzioni, per individuare il numero più probabile (MPN) corretto.†
9. Eliminare il terreno in accordo con le buone pratiche di laboratorio.
Italiano
Per ricevere assistenza tecnica contattare:
Europe: +00800 4339 9111
idexx.it/acqua
IDE
XX
Qu
anti
-Tra
y* /200
0 Ta
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la M
PN
# P
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Gra
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Pos
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# P
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Pic
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Po
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01
23
45
67
89
1011
1213
1415
1617
1819
2021
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24
0<
11.
02.
03.
04.
05.
06.
07.
08.
09.
010
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.117
.118
.119
.120
.221
.222
.223
.324
.3
11.
02.
03.
04.
05.
06.
07.
18.
19.
110
.111
.112
.113
.214
.215
.216
.217
.318
.319
.320
.421
.422
.423
.524
.525
.6
22.
03.
04.
15.
16.
17.
18.
19.
210
.211
.212
.213
.314
.315
.416
.417
.418
.519
.520
.621
.622
.723
.724
.825
.826
.9
33.
14.
15.
16.
17.
28.
29.
210
.311
.312
.413
.414
.515
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.618
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.127
.128
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26.
27.
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39.
310
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.513
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.124
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.6
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37.
38.
49.
410
.511
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.714
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.917
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.324
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.627
.728
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.0
66.
37.
48.
49.
510
.611
.612
.713
.814
.916
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.119
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.321
.422
.523
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.725
.826
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.029
.130
.231
.332
.4
77.
58.
59.
610
.711
.812
.813
.915
.016
.117
.218
.319
.420
.521
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.027
.128
.329
.430
.531
.632
.833
.9
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69.
710
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.216
.317
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.730
.832
.033
.134
.335
.4
99.
810
.912
.013
.114
.215
.316
.417
.618
.719
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.922
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.426
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.728
.930
.031
.232
.333
.534
.635
.837
.0
1011
.012
.113
.214
.415
.516
.617
.718
.920
.021
.122
.323
.424
.625
.726
.928
.029
.230
.331
.532
.733
.835
.036
.237
.438
.6
1112
.213
.414
.515
.616
.817
.919
.120
.221
.422
.523
.724
.826
.027
.228
.329
.530
.731
.933
.034
.235
.436
.637
.839
.040
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1213
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.615
.816
.918
.119
.320
.421
.622
.823
.925
.126
.327
.528
.629
.831
.032
.233
.434
.635
.837
.038
.239
.540
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.9
1314
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.118
.319
.520
.621
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.024
.225
.426
.627
.829
.030
.231
.432
.633
.835
.036
.237
.538
.739
.941
.242
.443
.6
1416
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.318
.519
.720
.922
.123
.324
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.726
.928
.129
.330
.531
.733
.034
.235
.436
.737
.939
.140
.441
.642
.944
.245
.4
1517
.518
.719
.921
.122
.323
.524
.725
.927
.228
.429
.630
.932
.133
.334
.635
.837
.138
.439
.640
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9.2
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9.1
3310
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914
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.714
13.6
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32.9
1986
.324
19.6
>24
19.6
One IDEXX DriveWestbrook, Maine 04092 USA
Quanti-Tray*/2000
† Descargue el software de IDEXX de determinación del NMP en la página idexx.com/mpngenerator para obtener resultados automatizados con Quanti-Tray.
*Quanti-Tray es una marca o marca registradas de IDEXX Laboratories, Inc. o sus filiales en los Estados Unidos y/o otros paises.
IntroducciónLos dispositivos Quanti-Tray*/2000 de IDEXX están diseñados para producir recuentos bacterianos cuantificados de muestras de 100 mL, al ser utilizadas con productos de reactivos de la de IDEXX. Agregue la mezcla de reactivo y muestra a un dispositivo Quanti-Tray/2000, séllelo en el Quanti-Tray* Sealer (Selladora) e incúbela según las instrucciones del reactivo. Luego cuente el número de celdas positivas y utilice la tabla de NMP adjunta para determinar el Número Más Probable (NMP).
Instrucciones para el usuario1. Sostenga en una mano el dispositivo
Quanti-Tray* en posición vertical, con el lado de las celdas orientado hacia la palma.
2. Apriete la parte superior del dispositivo Quanti-Tray de modo se doble hacia la palma.
3. Abra el dispositivo Quanti-Tray tirando de la lengüeta metálica del lado que contiene las celdas. Evite tocar el interior de la lengueta o del dispositivo.
4. Vierta la mezcla de reactivo y la muestra directamente dentro del dispositivo Quanti-Tray, evitando tocar la lengüeta. Golpear los pequeños pocillos 2 ó 3 veces para eliminar posibles burbujas de aire. Deja reposar la espuma.
5. Coloque el dispositivo Quanti-Tray lleno de la muestra sobre el portadispositivo de goma del selladora Quanti-Tray, orientando el lado de las celdas de plástico del dispositivo hacia abajo en el molde.
Español
Contacte con el servicio técnico en los siguientes teléfonos:
Europe: +00800 4339 9111
idexx.es/agua
6. Selle el dispositivo según las instrucciones del selladora.
7. Incube de acuerdo con las instrucciones del reactivo.
8. Cuente las celdas positivas. Para determinar el número más probable, recurra a la tabla NMP al dorso de esta hoja de instrucciones.†
9. Eliminar los consumibles conforme a las Buenas Prácticas de Laboratorio.
IDE
XX
Qu
anti
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0 Ta
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, núm
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19.
110
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04.
15.
16.
17.
18.
19.
210
.211
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.213
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16.
17.
28.
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210
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32.9
1986
.324
19.6
>24
19.6
One IDEXX DriveWestbrook, Maine 04092 USA
Quanti-Tray*/2000
† Laden Sie die Software „MPN Generator“ für automatisierte Quanti-Tray-Ergebnisse von idexx.com/mpngenerator herunter.
*Quanti-Tray ist eine Schutzmarke oder eine eingetragene Schutzmarke von IDEXX Laboratories, Inc. oder eines Tochterunternehmens von IDEXX in den Vereinigten Staaten und/oder in anderen Ländern.
EinführungQuanti-Tray*/2000 von IDEXX dienen zur quantitativen Bakterienzählung in 100-mL-Proben unter Verwendung von IDEXX -Reagenzien. Die Mischung aus Probe und Reagenz in einen Quanti-Tray/2000 geben, diesen in einem Quanti-Tray-Versiegelungsgerät verschließen und laut Reagenz-Packungsbeilage inkubieren. Anschließend die Anzahl der positiven Vertiefungen zählen und mit der beigefügten MPN-Tabelle die “Wahrscheinlichste Anzahl” (MPN) bestimmen.
Gebrauchsanweisung1. Quanti-Tray* mit einer Hand fassen
und senkrecht halten, wobei die Seite mit den Vertiefungen zur Handfläche zeigt.
2. Den oberen Teil des Quanti-Trays zusammendrücken, so daß sich der Träger zur Handfläche hin biegt.
3. Quanti-Tray öffnen, indem die Folienlasche von der Seite mit den Vertiefungen abgezogen wird. Die Innenseite der Folie oder des Trays nicht berühren.
4. Die Mischung aus Probe und Reagenz direkt in einen Quanti-Tray gießen. Dabei eine Berührung der Folienlasche vermeiden. Seitlich 2–3 mal an die kleinen Vertiefungen klopfen, um eventuelle Luftblasen zu entfernen. Schaum einwirken lassen.
5. Den mit der Probe gefüllten Quanti-Tray auf die Gummi-Trägerunterlage des Quanti-Tray-Versiegelungsgeräts legen. Die Seite mit den Vertiefungen (Kunststoff) des Quanti-Trays muß dabei nach unten zeigen.
6. Das Verschließen erfolgt laut Anweisungen für das Versiegelungsgerät.
7. Entsprechend den Reagenzanweisungen inkubieren.
8. Die positiven Vertiefungen zählen und mit Hilfe der MPN-Tabelle auf der Rückseite dieser Packungsbeilage die Wahrscheinlichste Anzahl (MPN) ermitteln.†
9. Entsorgen Sie die Quanti-Trays gemäß guter Laborpraxis.
