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Quantitative determination Of serum amylase activity Dr. Samah Kotb Lecturer of Biochemistry 2015 Cellular Biochemistry and metabolism 1
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Page 1: Quantitative determination Of serum amylase activity Dr. Samah Kotb Lecturer of Biochemistry 2015 Cellular Biochemistry and metabolism 1 CLS 331.

Quantitative determination Of serum amylase activity

Dr. Samah KotbLecturer of Biochemistry

2015

Cellular Biochemistry and metabolism 1

CLS 331

Page 2: Quantitative determination Of serum amylase activity Dr. Samah Kotb Lecturer of Biochemistry 2015 Cellular Biochemistry and metabolism 1 CLS 331.

Quantitative determination of

serum amylase activity

Page 3: Quantitative determination Of serum amylase activity Dr. Samah Kotb Lecturer of Biochemistry 2015 Cellular Biochemistry and metabolism 1 CLS 331.

• Amylase is an enzyme that changes complex sugars (starches) into simple sugars during digestion.

• Levels of amylase in the blood can be used to help diagnose and monitor diseases, such as diseases of the pancreas and salivary glands, or to determine whether the intestines have been damaged.

Amylase

Page 4: Quantitative determination Of serum amylase activity Dr. Samah Kotb Lecturer of Biochemistry 2015 Cellular Biochemistry and metabolism 1 CLS 331.

There are two kinds of amylase enzymes:

• Alpha-amylase (ptyalin), which is produced by the salivary glands.This enzyme begins starch digestion in the mouth and continues to work in the stomach.

• Pancreatic amylase, which is secreted by the pancreas into the small intestine. This enzyme continues the starch digestion process.

Page 5: Quantitative determination Of serum amylase activity Dr. Samah Kotb Lecturer of Biochemistry 2015 Cellular Biochemistry and metabolism 1 CLS 331.

• An amylase test measures the amount of this enzyme in a sample of blood taken from a vein or in a sample of urine.

Page 6: Quantitative determination Of serum amylase activity Dr. Samah Kotb Lecturer of Biochemistry 2015 Cellular Biochemistry and metabolism 1 CLS 331.

• Normally, only low levels of amylase are found in the blood or urine. But if the pancreas or salivary glands become damaged or blocked, more amylase is usually released into the blood and urine. In the blood, amylase levels rise for only a short time. In the urine, amylase may remain high for several days.

Page 7: Quantitative determination Of serum amylase activity Dr. Samah Kotb Lecturer of Biochemistry 2015 Cellular Biochemistry and metabolism 1 CLS 331.

• Salivary glands produce saliva, which moistens the mouth to help a person chew and swallow food. Saliva also contains substances (enzymes) that begin the breakdown of food.

Page 8: Quantitative determination Of serum amylase activity Dr. Samah Kotb Lecturer of Biochemistry 2015 Cellular Biochemistry and metabolism 1 CLS 331.

There are four pairs of salivary glands:

• The parotid glands are located between the ear and the jaw.

• The submandibular glands are located under the jaw.

Page 9: Quantitative determination Of serum amylase activity Dr. Samah Kotb Lecturer of Biochemistry 2015 Cellular Biochemistry and metabolism 1 CLS 331.

• The sublingual glands are located on the floor of the mouth under the tongue.

• The buccal glands are not shown in this picture. They are located in the mucous membrane lining the cheeks and mouth. These glands produce only a small amount of saliva.

Page 10: Quantitative determination Of serum amylase activity Dr. Samah Kotb Lecturer of Biochemistry 2015 Cellular Biochemistry and metabolism 1 CLS 331.

Salivary Glands

Page 11: Quantitative determination Of serum amylase activity Dr. Samah Kotb Lecturer of Biochemistry 2015 Cellular Biochemistry and metabolism 1 CLS 331.

Why It Is Done

A test for amylase is done to:• Find pancreatitis and other pancreatic diseases.

• See if the treatment for pancreatitis and other pancreatic diseases is working.

• Check swelling and inflammation of the salivary glands.

Page 12: Quantitative determination Of serum amylase activity Dr. Samah Kotb Lecturer of Biochemistry 2015 Cellular Biochemistry and metabolism 1 CLS 331.

How To Prepare

To prepare for an amylase test:• For a blood test for amylase, do not eat or drink

anything except water for at least 2 hours before having the test.

• For a 24-hour urine test for amylase, be sure to drink enough fluids during the test to prevent dehydration.

Page 13: Quantitative determination Of serum amylase activity Dr. Samah Kotb Lecturer of Biochemistry 2015 Cellular Biochemistry and metabolism 1 CLS 331.

• Many medicines may affect the results of this test.

• Be sure to tell your doctor about all the non-

prescription and prescription medicines you take.

Page 14: Quantitative determination Of serum amylase activity Dr. Samah Kotb Lecturer of Biochemistry 2015 Cellular Biochemistry and metabolism 1 CLS 331.

How It Is Done

Blood test• Wrap an elastic band around your upper arm to

stop the flow of blood. This makes the veins below the band larger so it is easier to put the needle in the vein.

Page 15: Quantitative determination Of serum amylase activity Dr. Samah Kotb Lecturer of Biochemistry 2015 Cellular Biochemistry and metabolism 1 CLS 331.

• Clean the needle site with alcohol.

• Put the needle in the vein.

• Attach a tube to the needle to fill it with blood.

Page 16: Quantitative determination Of serum amylase activity Dr. Samah Kotb Lecturer of Biochemistry 2015 Cellular Biochemistry and metabolism 1 CLS 331.

• Remove the band from your arm when enough blood is collected.

• Put a gauze pad or cotton ball over the needle site as the needle is removed.

Page 17: Quantitative determination Of serum amylase activity Dr. Samah Kotb Lecturer of Biochemistry 2015 Cellular Biochemistry and metabolism 1 CLS 331.
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principle

Page 19: Quantitative determination Of serum amylase activity Dr. Samah Kotb Lecturer of Biochemistry 2015 Cellular Biochemistry and metabolism 1 CLS 331.

Reagents

Note:2-(N-morpholino)ethanesulfonic acid – MES buffer

Page 20: Quantitative determination Of serum amylase activity Dr. Samah Kotb Lecturer of Biochemistry 2015 Cellular Biochemistry and metabolism 1 CLS 331.

Normal Range

Page 21: Quantitative determination Of serum amylase activity Dr. Samah Kotb Lecturer of Biochemistry 2015 Cellular Biochemistry and metabolism 1 CLS 331.

Procedure

Page 22: Quantitative determination Of serum amylase activity Dr. Samah Kotb Lecturer of Biochemistry 2015 Cellular Biochemistry and metabolism 1 CLS 331.

Calculation

Page 23: Quantitative determination Of serum amylase activity Dr. Samah Kotb Lecturer of Biochemistry 2015 Cellular Biochemistry and metabolism 1 CLS 331.

Sample

Page 24: Quantitative determination Of serum amylase activity Dr. Samah Kotb Lecturer of Biochemistry 2015 Cellular Biochemistry and metabolism 1 CLS 331.

Clinical significance

Page 25: Quantitative determination Of serum amylase activity Dr. Samah Kotb Lecturer of Biochemistry 2015 Cellular Biochemistry and metabolism 1 CLS 331.
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