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Racing Bacterial Cells in Microfluidic Gradients

Date post: 30-Dec-2015
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Racing Bacterial Cells in Microfluidic Gradients. in order to measure chemotactic efficiency of isogenic bacteria population in correlation to their morphology. Racing Bacterial Cells in Microfluidic Gradients. - PowerPoint PPT Presentation
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Racing Bacterial Cells in Microfluidic Gradients in order to measure chemotactic efficiency of isogenic bacteria population in correlation to their morphology
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Page 1: Racing Bacterial Cells in Microfluidic Gradients

Racing Bacterial Cells in Microfluidic Gradients

in order to measure chemotactic efficiency of isogenic bacteria population in correlation to their morphology

Page 2: Racing Bacterial Cells in Microfluidic Gradients

Racing Bacterial Cells in Microfluidic Gradients

in order to measure chemotactic efficiency of isogenic bacteria population in correlation to their morphology

Why:

Length variation is observed in isogenic bacteria population

Page 3: Racing Bacterial Cells in Microfluidic Gradients

Racing Bacterial Cells in Microfluidic Gradients

in order to measure chemotactic efficiency of isogenic bacteria population in correlation to their morphology

Why:

Length variation is observed in isogenic bacteria population

Does length variation have any functional role? → e.g. enhanced/diminshed motility?

Page 4: Racing Bacterial Cells in Microfluidic Gradients

Racing Bacterial Cells in Microfluidic Gradients

in order to measure chemotactic efficiency of isogenic bacteria population in correlation to their morphology

Why:

Length variation is observed in isogenic bacteria population

Does length variation have any functional role? → e.g. enhanced/diminshed motility?

Aim: Physical model of how cell size and number of flagella relate to swimming speeds and efficiency in chemotaxis

Page 5: Racing Bacterial Cells in Microfluidic Gradients

How:

Build microfluidics chamber using PDMS based soft-lithography

Page 6: Racing Bacterial Cells in Microfluidic Gradients

How:

Build microfluidics chamber using PDMS based soft-lithography Create nutrition gradient in chamber to induce chemotaxis (adding sugar)

→ Quantitative measurement of gradient by adding dye in same conc. → Simulating gradient with physics modeling program

chemoattractant bacteria

Page 7: Racing Bacterial Cells in Microfluidic Gradients

How:

Build microfluidics chamber using PDMS based soft-lithography Create nutrition gradient in chamber to induce chemotaxis (adding sugar)

→ Quantitative measurement of gradient by adding dye in same conc. → Simulating gradient with physics modeling program

Recording bacterias with DIC timelapse microscopy Identify single cells and measure their motion tracks (Matlab) as well as size

chemoattractant bacteria

Page 8: Racing Bacterial Cells in Microfluidic Gradients

How:

Build microfluidics chamber using PDMS based soft-lithography Create nutrition gradient in chamber to induce chemotaxis (adding sugar) → Quantitative measurement of gradient by adding dye in same conc.

→ Simulating gradient with physics modeling program Recording bacterias with DIC timelapse microscopy Identify single cells and measure their motion tracks (Matlab) as well as size

Page 9: Racing Bacterial Cells in Microfluidic Gradients

Build microfluidics chamber using PDMS based soft-lithography Create nutrition gradient in chamber to induce chemotaxis (adding sugar) → Quantitative measurement of gradient by adding dye in same conc.

→ Simulating gradient with physics modeling program Recording bacterias with DIC timelapse microscopy Identify single cells and measure their motion tracks (Matlab) as well as size

~1.5 µm thickness~ 3 µm spacing

Page 10: Racing Bacterial Cells in Microfluidic Gradients
Page 11: Racing Bacterial Cells in Microfluidic Gradients

Positions of tracked beads

Page 12: Racing Bacterial Cells in Microfluidic Gradients

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