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Rajkumar Misra

University of Delaware Phone: +1-302-510-2126

Materials Science & Engineering - University of Delaware, Dupont Hall, Lab 223 Newark, DE

19716, UNITED STATES.

Email: rajkumar@udel.edu or rajkumarmisra96@gmail.com

PERSONAL INFORMATION:

Gender: Male Nationality: Indian

Birth: 05th December 1988 Marital Status: Unmarried

EDUCATION:

2018 2018-present Postdoctoral Researcher in Materials Science and

Engineering, University of Delaware, Delaware, Advisor: Prof.

Darrin Pochan and Prof. Chris Kloxin.

2018 Ph. D in Chemistry, Department of chemistry,

Indian Institute of Science Education and Research (IISER), Pune,

India. “Exploring stereochemical constrained gamma amino acid in the

design of peptide foldamers and supramolecular assembly”

Advisor: Dr. H. N. Gopi, Associate Professor of Chemistry.

2010-2012 Master of Science (Organic Chemistry)

Department of Chemistry Indian Institute of Technology Guwahati,

Guwahati, 781039. Assam, India.

2006-2010 Bachelor of Science (Chemistry)

Midnapore College, West Bengal, India.

TEACHING EXPERIENCE:

2012- 2014 Teaching assistant

Served as teaching assistant during doctoral program for three different

undergraduate courses in chemistry (including two practical course) and

problem solving sessions at Indian Institute of Science Education and

Research (IISER), Pune. India

Instructors: Dr. H. N. Gopi and Dr. Raghavendra Kikkeri

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MENTORING EXPERIENCE:

2012-2017 4-6-week undergraduate research internships in “Synthetic Peptide

Chemistry”, IISER, Pune, India.

Students: Shibani Sharma, Abhijith Saseendran, Veeresh Raina, Saikat

Pahan, Sanjit Dey, Rahi Reja.

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HONORS AND AWARDS:

2011 Graduate Aptitude Test in Engineering (GATE) Award, India

National level award that allows candidate to conduct doctoral research

in India.

2011 Council of Scientific and Industrial Research (CSIR) Fellowship and

National Eligibility Test (NET) Award, India

A joint CSIR-UGC (University Grant Commission) award which offers

fellowship to the candidate for five years to conduct doctoral research in

India and certify that candidate is eligible to take lectureship in chemical

sciences at the universities in India.

CONFERENCE PROCEEDINGS:

05/2013 International Meeting on Chemical Biology, Pune, India -Poster Presentation

12/2015 The 7th Peptide Engineering Meeting(PEM-2015) -Awarded Best Poster Presenter

03/2016 The international conference on nanoscience and technology 2016 (ICONST-

2016)- -Poster Presentation

RESEARCH EXPERTISE:

• Strong background in the synthesis of non-proteinogenic amino acids and unnatural

amino acids with proteinogenic side-chains.(β-amino acids, γ-amino acids, α,β-

dehydro γ-amino acids, β-keto-γ-amino acids and statins) and their usage in the de

novo designed secondary and super-secondary structures.

• Extensive experience in design and solution/solid phase peptide synthesis.

• Structural analysis of peptides.

• Experienced in design, synthesis and characterisation of peptide system that self

assembles into supramolecular structures like hydrogel, nanotubes and vesicles.

• Experienced in synthesis of liposomes (nano-vesicles) and kinetics studies of

liposome encapsulated drug release.

• Developed new synthetic methodologies for peptide bond synthesis and synthesis of

-amino acids.

• Routine experience in purification of peptides of various lengths using HPLC.

TECHNICAL EXPERTISE:

• NMR: Extensive experience for solving the secondary structural conformation of

peptides and hybrid foldamers using 2D NMR.

• SC-XRD: Experienced in operation of single crystal X- ray diffractometer and

solving the crystal structures of small peptides.

• Isothermal Titration calorimeter (ITC): Skilled to operate ITC instrument (iTC200

from Microcal) efficiently and carry out thermodynamics of peptide ligand

interactions.

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• Peptide synthesizer: Extensive experience in synthesis of peptides on solid phase

using peptide synthesizer

• MALDI TOF/TOF: Routinely operated MALDI TOF/TOF instrument to characterise

synthesized peptides and mini-proteins.

• CD: Experienced to operate CD instrument to interpret secondary structures of

peptides and proteins.

• HPLC: Extensively used the HPLC for purification of various peptides and mini-

proteins.

• AFM, SEM and TEM: Good experience in sample preparation and morphology

interpretation of peptide superstructures using these microscopic techniques.

• Rheometry: Experienced in characterization of visco-elastic properties of peptide

hydrogel.

HIGHLIGHTS:

• Published over 8 research papers in international peer-reviewed journals with fourth

first author contributions.

• Over 5th years of solid research experience in synthetic peptide chemistry.

• Strong expertise in design and synthesis of peptide biomaterials.

• Designed and synthesized various protein secondary and super-secondary structures.

such as helices, β-sheet, loop, turns, coiled-coils, β-hairpin and multi-stranded sheets.

• Experienced in design and enantiopure synthesis of unnatural amino acids (β-amino

acids, γ-amino acids) and their use in peptide foldamers.

• Skilled in various analytical techniques like FT-IR, NMR (1D and 2D), single crystal

XRD, MALDI-TOF/TOF, HPLC, CD, SEM, TEM, AFM, Rheometry and ITC

(Isothermal Titration Calorimetry).

• Skilled in purification of hydrophobic peptides.

• Experienced in team-oriented work.

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PUBLICATIONS:

1. R. Misra, K. M. P. Raja, H.-J. Hofmann, H. N. Gopi “Modulating the structural properties

of alpha,gamma-hybrid peptides by alpha-amino acid residues Uniform 12-helix versus

"mixed" 12/10-helix’’ Chem. Eur. J. DOI: 10.1002/chem.201703871

2. R. Misra, A. Saseendran, G. George, K. Veeresh, K. M. P. Raja, S. Raghothama, H.-J

Hofmann, and H. N. Gopi “Structural Dimorphism of Achiral α,γ-Hybrid Peptide Foldamers:

Coexistence of 12- and 15/17-Helices’’ Chem. Eur. J. 2017, 23, 3764

3. R. Misra, A. Sharma, A. Shiras, and H. N Gopi “Backbone Engineered γ-Peptide

Amphitropic Gels for Immobilization of Semiconductor Quantum dots and 2D Cell Culture’’

Langmuir, 2017, 33 , 7762.

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4. R. Misra, R. M. Reja, L. V. Narendra, G. George, S. Raghothama and H. N. Gopi

“Exploring structural features of folded peptide architectures in the construction of

nanomaterials’’ Chem. Commun., 2016, 52, 9597

5. A. Bandyopadhyay, R. Misra and H. N. Gopi “Structural features and molecular

aggregations of designed triple-stranded β-sheets in single crystals’’ Chem. Commun.,

2016, 52, 4938

6. S. V. Jadhav, R. Misra and H. N. Gopi “Foldamers to nanotubes: influence of amino acid

side chains in the hierarchical assembly of α,γ4 hybrid peptide helices’’ Chem. Eur. J. 2014,

20, 16523

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7. S.V. Jadhav, R. Misra, S. K. Singh, and H. N. Gopi “Efficient access to enantiopure γ4-

amino acids with proteinogenic side-chains and structural investigation of γ4-Asn and γ4-Ser in

hybrid peptide helices’’ Chem. Eur. J. 2013, 19, 16256

8. R. M Reja, M. Khan, S. K Singh, R. Misra, A. Shiras, H. N Gopi. “pH sensitive coiled

coils: a strategy for enhanced liposomal drug delivery’’ Nanoscale. 2016, 8, 5139

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9. R. Misra, G. George, A. Saseendran, S. Raghothama, H. N. Gopi “Ambidextrous α,-

Hybrid Peptide Foldamers with Reversal of Helix Directionality’’ Submitted

10. R. Misra, G. George, R. M. Reja, S. Dey, S. Raghothama, H. N. Gopi “Unique 11 helical

folding from β, -hybrid peptide with reversal of hydrogen bonding directionality’’ submitted

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11. R. Misra, S. Dey, H. N. Gopi “Artificial β-Double Helices from Achiral -Peptides’’

Angew Chem Int Ed Engl. 2018 Doi: 10.1002/anie.201711124

12. R. Misra, A. Saseendran , H. N. Gopi “Shortest hybrid helices design with high population

of beta branch amino acid and their unprecedented metal driven supramolecular

assembly’’submitted

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13. R. Misra, S. Dey, H. N. Gopi “Importance of aromatic-aromatic interaction in the hybrid

peptide foldamer self-assembly’’ under preparation