Recovering ancient grapevines varieties in
the Spanish provinces of Alicante and
Valencia
XII International Conference on Grapevine Breeding and Genetics (Bordeaux, July 15-20, 2018)
A. Yuste , F. Martínez-Gil, S. García-Martínez, D. López Lluch; R. Peiró
R, C. Jiménez, J.J. Ruiz and C. Gisbert
MINECO PROJECT CGL2015-70843-R FEDER FUNDS
Other researchers collaborating to reach this goal are from
Comunitat Valenciana
PROTECTED DESIGNATION OF ORIGIN (PDO)
Alt de BenimaquiaDenia
Wine elaboration VII bc century BC
XII International Conference on Grapevine Breeding and Genetics, Bordeaux, 2018
Protected Geographical
Indication Castellón
Identitity Synonymies Homonymies Variability
Germplams bank
SSR profiles
In vitro storage Virus cleaning x
XII International Conference on Grapevine Breeding and Genetics, Bordeaux, 2018 PROJECT CGL2015-70843-R
Grumer Moscatell Moscatel del terreno Moscatel d’ Alfàbega Gustico de Elche Moscatel Dulce Moscatel de Alicante
Muscat d’Istamboul (variety 17493 in VIVC) (Valencí blanc x Muscat of Alexandria; Lacombe et al. 2013 ; Mena et al. 2014)
Gisbert et al. 2018; Peiró et al. 2018; Jiménez et al. 2019
Raïm del Clotet
Some of the synonymies found
Botó de Gall accessions Ahmeur bou Ahmeur (variety 140 in VIVC)
XII International Conference on Grapevine Breeding and Genetics, Bordeaux, 2018
Esclafagerres
New SSR profiles i.e. Localization of varieties in risk of disapearance
Planta Mula, Valencí negre, Botó de Gall, Arcos, Forcallat, Verdilet, Mamella de vaca, Raïm del clotet, Esclafagerres, etc.
Analysis of variability
‘Monastrell’ (Mourvedre) ‘Moscatel de Alejandría’ (Muscat of Alexandria)
AFLPs/Peiró et al. 2018. Spanish Journal of Agricultural Research vol.56(2)
XII International Conference on Grapevine Breeding and Genetics, Bordeaux, 2018
GBS/46 accessions, 93.466 SNPs, Neighbour-Joining (NJ) bootstrap 1000. Reference clon MOU-369
Morenillo
Carignan
NI-5
Rojal Tinta
Planta Nova-1
Planta Nova-2
Alicante Henry Bouchet
Ullet de Perdiu
Alcañon
Verdil-1
Verdil-2
Mari Sancho
Trepadell
Planta Fina
REF Planta Fina
Alarije
Merseguera
Bobal
REF Bobal
Planta Mula
Morsí (Sbaa Tolba)
Macabeo
Xarello
REF Esclafagerres
Tortosina
Valencí Negre
Valencí Blanc-3
Valencí Blanc-1
Valencí Blanc-2
NI-1
REF Bonicaire
Forcallat Tinta
Arcos
Montalbana
REF Benicarló
Monastrell
REF Monastrell
Raïm del Clotet
NI-3
NI-4
Morastell
REF Pampolat
Crujidera (Marufo)
REF Gateta
Mondragón
NI-2
Botó de Gall (Ahmeur bou Ahmeur)
Mamella de Vaca
Afus Ali-1 (Rosetti)
Afus Ali-2 (Rosetti)
Alfonse Lavalleé
Cardinal
Cornichon Blanc
Dominga
Grumer Moscatell (Muscat d’Istanbul)
Muscat of Alexandria
REF Moscatel E-110
Moscatel de Hamburgo
Italia
0.05
SSRs/Jiménez et al. 2018/9
53% chlorotype A 23% chlorotype D 13% chlorotype B 11 % chlorotype C
Inter- and
Intra- varietal
variability
Analysis of variability
‘Monastrell’ (Mourvedre) ‘Moscatel de Alejandría’ (Muscat of Alexandria)
Peiró et al. 2018. Spanish Journal of Agricultural Research vol.56(2)
XII International Conference on Grapevine Breeding and Genetics, Bordeaux, 2018
46 accesions, 93.466 SNPs, Neighbour-Joining (NJ) bootstrap 1000. Reference clon MOU-369
Morenillo
Carignan
NI-5
Rojal Tinta
Planta Nova-1
Planta Nova-2
Alicante Henry Bouchet
Ullet de Perdiu
Alcañon
Verdil-1
Verdil-2
Mari Sancho
Trepadell
Planta Fina
REF Planta Fina
Alarije
Merseguera
Bobal
REF Bobal
Planta Mula
Morsí (Sbaa Tolba)
Macabeo
Xarello
REF Esclafagerres
Tortosina
Valencí Negre
Valencí Blanc-3
Valencí Blanc-1
Valencí Blanc-2
NI-1
REF Bonicaire
Forcallat Tinta
Arcos
Montalbana
REF Benicarló
Monastrell
REF Monastrell
Raïm del Clotet
NI-3
NI-4
Morastell
REF Pampolat
Crujidera (Marufo)
REF Gateta
Mondragón
NI-2
Botó de Gall (Ahmeur bou Ahmeur)
Mamella de Vaca
Afus Ali-1 (Rosetti)
Afus Ali-2 (Rosetti)
Alfonse Lavalleé
Cardinal
Cornichon Blanc
Dominga
Grumer Moscatell (Muscat d’Istanbul)
Muscat of Alexandria
REF Moscatel E-110
Moscatel de Hamburgo
Italia
0.05
Jiménez et al. 2018/9
53% chlorotype A 23% chlorotype D 13% chlorotype B 11 % chlorotype C
Inter- and
Intra- varietal
variability
Development of protocols for virus cleaning
Virus analysis U
E20
02
/11
/CE
XII International Conference on Grapevine Breeding and Genetics, Bordeaux, 2018
Grapevine fanleaf virus (GFLV) Grapevine leafroll associated virus 1 (GLRaV-1) Grapevine leafroll associated virus 3 (GLRaV-3) Grapevine fleck virus (GFkV) Arabis mosaic virus (ArMV) x
Meristem culture Somatic embryogenesis
High incidence 35% simple 37% doble 23% triple
Development of protocols for in vitro storage
XII International Conference on Grapevine Breeding and Genetics, Bordeaux, 2018
Thank you/Merci
XII International Conference on Grapevine Breeding and Genetics, Bordeaux, 2018
