ReliaPrep(TM) LV 32 HSM Instrument Technical … · LV 32 HSM Instrument provides all-in-one...

T e c h n i c a l M a n u a l

ReliaPrep™ LV 32 HSM InstrumentINSTRUCTIONS FOR USE OF PRODUCT A1715.

PRINTED IN USA Part# TM326Revised 3/13

Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.comPrinted in USA. Part# TM326Revised 3/13 Page 1

1. Introduction...................................................................................................................................................................2A. Intended Use of the ReliaPrep™ LV 32 HSM Instrument ...........................................................................2B. ReliaPrep™ LV 32 HSM Instrument Specifications ......................................................................................3C. Product Components .........................................................................................................................................3D. Inspection.............................................................................................................................................................3E. Precautions...........................................................................................................................................................5F. Environmental Requirements ...........................................................................................................................7

2. Hardware Overview.....................................................................................................................................................7

3. Unpacking and Setting Up the ReliaPrep™ LV 32 HSM Instrument...............................................................9A. Setup .....................................................................................................................................................................9B. Shutdown.............................................................................................................................................................9

4. Operating the ReliaPrep™ LV 32 HSM Instrument ...........................................................................................10A. Main Menu Navigation....................................................................................................................................10B. Sample Purification Using the ReliaPrep™ Large Volume HT gDNA Isolation System .....................10C. Remote Menu Option.......................................................................................................................................11D. ReliaPrep™ Menu Option ...............................................................................................................................11E. Manual Menu Option.......................................................................................................................................11F. Setup Menu Option ..........................................................................................................................................12

5. ReliaPrep™ LV 32 HSM Instrument Cleaning and Maintenance ...................................................................13

6. Troubleshooting .........................................................................................................................................................15

7. Appendix......................................................................................................................................................................17A. Instrument Startup, Diagnostics and Errors.................................................................................................17B. Updating Firmware ..........................................................................................................................................17C. Instrument Disposal .........................................................................................................................................18D. Returning the ReliaPrep™ LV 32 HSM Instrument and Accessories ......................................................18E. Certificate of Decontamination.......................................................................................................................20F. Warranty Information ......................................................................................................................................21G. Related Products, Warranties and Service Agreements .............................................................................22

All technical literature is available on the Internet at: www.promega.com/protocols/Please visit the web site to verify that you are using the most current version of this Technical Manual.

Please contact Promega Technical Services if you have questions on the use of this product. Email: [email protected]

ReliaPrep™ LV 32 HSM Instrument

Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.comPart# TM326 Printed in USA.Page 2 Revised 3/13

1. Introduction

The ReliaPrep™ Large Volume Heater Shaker Magnet Instrument (ReliaPrep™ LV 32 HSM) is designedto perform all of the functions necessary for the processing of magnetic resin-based purifications incommon 50ml conical tubes. With its ability to heat, shake and apply a magnetic field, the ReliaPrep™LV 32 HSM Instrument provides all-in-one processing capabilities for a variety of large volumepurification chemistries in either a manual or automated format. The instrument uses standard 50mlconical tubes, magnets and reagent-based paramagnetic particles (PMPs). The PMPs provide a mobilesolid phase that optimizes capture, washing and elution of biological target molecules.

Initially designed to run the Promega ReliaPrep™ Large Volume HT gDNA Isolation System (Cat.# A1751), the ReliaPrep™ LV 32 HSM Instrument is supplied with preprogrammed isolationmethods for processing up to 32 samples of human whole blood in approximately 2–3.5 hours,depending on sample type and number. Samples are processed in a semi-automated method with theuser dispensing and aspirating reagents from the samples as directed by the LCD screen. Theprogrammed methods instruct the instrument when to apply shaking, heating and magnetic forces tothe sample tubes for cell lysis, capture and elution of DNA. The ReliaPrep™ LV 32 HSM Instrumentscan be controlled simultaneously on a robotic liquid handling workstation to fully automate processingof blood samples. The number of samples per instrument run depends on the number of Heater ShakerMagnets (HSMs) integrated. Each HSM is able to accommodate 1-32 samples. In both manual andautomated processing, high-quality and high-yield DNA is obtained for direct use in a variety ofdownstream analytical applications.

1.A. Intended Use of the ReliaPrep™ LV 32 HSM Instrument

The ReliaPrep™ LV 32 HSM Instrument is designed for use in combination with the ReliaPrep™ LargeVolume HT gDNA Isolation System for the isolation of genomic DNA from 3, 6, or 10ml of humanwhole blood. When controlled by a robotic liquid handling workstation, the ReliaPrep™ DNA isolationchemistry is scalable for samples from 3–10ml. While designed to work with the ReliaPrep™ LargeVolume HT gDNA Isolation System, the ReliaPrep™ LV 32 HSM Instrument has applicability to avariety of large volume paramagnetic resin-based purification chemistries. Use of this instrument withchemistries other than the ReliaPrep™ Large Volume HT gDNA Isolation System may requiredevelopment and optimization of manual or automated methods by the user.


1.B. ReliaPrep™ LV 32 HSM Instrument Specifications

Processing Time (blood genomic DNA): 2–3.5 hours (depending on sample type and number)Number of Samples: up to 32Weight:

Main Shaker: 42.33lb (19.2kg)Power Supply: 5.93lb (2.69kg)

Dimensions: (W × L× H)Main Shaker: 9.25 × 17.1 × 5.5 inches (234.95 × 449.48 × 139.7mm)Power Supply: 5.5 × 15.875 × 2.25 inches (139.7 × 403.22 × 57.15mm)

Power Requirements: Main Shaker: 24 V DC, 20 APower Supply: 100–240VAC, 50–60Hz

Shaking Orbit: 4mmAccepts tubes with an OD of no more than 29mm

1.C. Product Components

Product Quantity Cat.#ReliaPrep™ LV 32 HSM Instrument 1 each A1715Includes:

• 1 ReliaPrep™ LV 32 HSM Instrument• 1 ReliaPrep™ Tube Rack• 1 ReliaPrep™ Tube Rack Stand• 1 Power Supply for use with ReliaPrep™ LV 32 HSM Instrument• 1 Power Cable (blue)• 1 RS-232 Cable • 1 USB-to-Serial Converter• 1 Power Cord (black)• 1 Setup Guide

1.D. Inspection

Upon receiving your ReliaPrep™ LV 32 HSM Instrument, please inspect the package carefully to makesure all items are present and undamaged. See Figures 1 and 2 for views of the instrument andaccessories.


Figure 1. Power Supply, ReliaPrep™ LV 32 HSM Instrument, and Tube Rack on Tube Rack Stand (from left toright).

Figure 2. ReliaPrep™ LV 32 HSM Accessories. Power Cord (black), Instrument Power Cable (blue), RS-232 Cable,USB-to-serial converter (not shown), Set Up Guide (not shown). Note: Tube Rack and Power Cord (black) areshipped in a separate box.

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1.E. PrecautionsSafety Symbols and Markings

Danger. Hazardous voltage. Risk of electricalshock.

Danger. Tension dangereuse. Risque de chocélectrique.

Warning. Risk of personal injury to the operatoror a safety hazard to the equipment orsurrounding area.

Avertissement. Risque de préjudice corporel pourl'opérateur ou d'accident avec l'instrument oul'entourage.

Warning. Pinch point hazard. Avertissement. Risque de pincement.

Warning. Hot surface. Burn hazard. Avertissement. Surface chaude. Risque de brûlure.

Warning. Lifting hazard. Avertissement. Risque lors du soulèvement.

Warning. Biohazard. Avertissement. Risque biologique.

Warning. Strong magnet. Can be harmful topacemaker wearers.

Attention. Aimant puissant. Peut être dangereux pourdes porteurs de pacemaker.

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Warning. Magnet may interfere with pacemakersand other devices. Pacemaker wearers stay back30cm (12 in).

Attention. L'aimant peut avoir des effets sur lespacemakers et autres dispositifs. Les porteurs depacemaker doivent rester à 30 cm de l'appareil.

It is important to understand and follow all lawsregarding the safe and proper disposal of electricalinstrumentation. Please contact your local PromegaRepresentative for disposal of the instrument andpower supply. Please follow your institutionalrequirements for disposal of the accessories.

Avertissement. Il est important de comprendre et derespecter toutes les lois relatives à la destruction sûre etcorrecte des appareils électriques. Veuillez contactervotre représentant Promega local concernant ladestruction de l’appareil. Veuillez respecter les exigencesde votre établissement concernant la destruction desaccessoires.

Catalog Number Numéro de catalogue

Serial Number Numéro de série

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This product has been tested to the requirements ofCAN/CSA-C22.2 No. 61010-1, second edition,including Amendement 1, or a later version of thesame standard incorporating the same level oftesting requirements.

Ce produit a été testé en accord avec les exigences deCAN/CSA-C22.2 No. 61010-1, 2eme Edition, ci-inclusl'amendement 1, ou un version ultérieur du mêmerèglement incorporant les mêmes conditions d'essai..


Changes or modifications to this unit not expressly approved by the party responsible for compliance could void theuser’s authority to operate the equipment.This equipment has been designed and tested to CISPR 11 Class A. In a domestic environment it may cause radiointerference, in which case, you may need to take measures to mitigate the interference.Do not use this device in proximity to sources of strong electromagnetic radiation (e.g., unshielded intentional RFsources), as these may interfere with the proper operation.Use only the power supply provided with the instrument. There are no user-serviceable parts in the equipment.Removing the instrument or power supply casing will void the warranty.IMPORTANT SAFETY INSTRUCTIONS. SAVE THESE INSTRUCTIONS.

Safety Precautions Mesures de SécuritéDo not use this equipment for anything otherthan its intended use.

Ne pas utiliser cet instrument à d'autres fins quecelle pour laquelle il a été conçu.

Always disconnect the power before cleaningor performing routine maintenance.

Toujours débrancher le cordon d'alimentation avantd'effectuer le nettoyage ou l'entretien de routine.

Do not disassemble unit. There are no user-serviceable parts in the equipment.

Ne pas démonter l'appareil. Il n'existe aucune piècepouvant être changée par l'utilisateur.

If the equipment is used in a manner otherthan that specified by Promega, the protectionprovided by the equipment may be impaired.

Si cet appareil est utilisé à d'autres fins que cellespour lesquelles il a été conçu, la protection fournitpourrait en être diminuée.

Keep hands clear of shaking platform. Eloignez les mains de la plateforme agitante.

The internal surfaces of the tube holders maybecome hot. Do not touch.

Les surfaces internes des portoirs de tubes peuventêtre chaudes. Ne pas toucher.

To avoid muscle strain or back injury, uselifting aids and proper lifting techniques whenremoving or replacing the instrument. TheReliaPrep™ LV 32 HSM Instrument weighs19.2kg (43.3lb) and should be handled by twopeople. The packaged instrument weighs 40kg(88lb).

Pour éviter des claquages musculaires ou se faire malau dos, utiliser du matériel permettant de soulever etdes techniques de soulèvement correctes lors duretrait ou du repositionnement de l'appareil. LeReliaPrep™ LV 32 HSM Instrument pèse 19,2kg anddoit être manipulé par deux personnes. L'appareilemballé pèse 40kg.

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Equipment can be hazardous due to the use ofchemical and biohazardous substances.

L'appareil peut être dangereux dû à l'utilisation desubstances chimiques ou dangereuses.

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Warning. Strong magnet. Can be harmful topacemaker wearers.

Attention. Aimant puissant. Peut être dangereuxpour des porteurs de pacemaker.

Warning. Magnet may interfere withpacemakers and other devices. Pacemakerwearers stay back 30cm (12 in).

Attention. L'aimant peut avoir des effets sur lespacemakers et autres dispositifs. Les porteurs depacemaker doivent rester à 30cm de l'appareil.


1.F. Environmental Requirements (operation, shipping and storage conditions)

Power Requirements: 100–240VAC, 50–60HzTemperature: 5-40°CHumidity: up to 80% relative humidity

The ReliaPrep™ LV 32 HSM Instrument is intended for indoor use only. If liquid gets inside theinstrument, it may damage the electronics. Install in a location that meets the following criteria:

• Install on a sturdy, dust-free, level surface.• Choose a location that has good air circulation and is not exposed to direct sunlight.• Avoid noisy electrical power sources (e.g., power generators).• Do not install in a location where there is large temperature variability or high humidity.• Position the instrument so that it is easy to unplug from the power source.•Do not place the power supply in areas where it may come in contact with liquid.• Do not place next to heat sources.• Do not use near flammable gases or liquids.• For optimal performance, we recommend equilibrating the instrument to room temperature before

operating.

2. Hardware Overview

Figure 3. Front of the instrument showing screen display, control buttons and instrument on/off switch.

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Figure 4. Rear of the instrument showing connection for the blue power cable and the RS-232 Cable port.

Figure 5. Rear of the Power Supply showing power connection and fuse.

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Connection forblue Power Cableto Instrument

Connection forBlack Power Cord

FuseOn/OffSwitch

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Connection forblue Power Cable

to Power Supply

RS-232 port

RS-232 port(unused)


3. Unpacking and Setting Up the ReliaPrep™ LV 32 HSM Instrument

3.A. Setup

1. Remove the ReliaPrep™ LV 32 HSM Instrument, Power Supply and accessories from theshipping containers.

Important: Save the packaging material (including the shipping pallet) in case the equipmentneeds to be returned for service or repair at a later date.

2. Check that all parts have been included. Refer to Sections 1.C and 1.D for a list of parts.

3. Set the ReliaPrep™ LV 32 HSM Instrument on a flat, level surface in a dust-free location withreasonable air circulation.

4. Ensure that the power switches are in the off position. The power switches are located on thefront of the ReliaPrep™ LV 32 HSM Instrument and the back of the Power Supply (Figures 3and 5, respectively).

5. Connect the ReliaPrep™ LV 32 HSM Instrument to the Power Supply using the blue PowerCable by plugging the cable into the back of the ReliaPrep™ LV 32 HSM Instrument (Figure4) and the back of the Power Supply (Figure 5).Important: Place the power supply in a dry location away from possible liquid spills.

6. Plug the black Power Cord into the back of the Power Supply (Figure 5). Plug the Power Cordinto a grounded wall outlet. See Section 1.B for power requirements.

7. Turn on the power switch located on the rear of the Power Supply (Figure 5). Turn on thepower switch on the front of the ReliaPrep™ LV 32 HSM Instrument (Figure 3).

8. The instrument will now display the firmware version number and operational setting andwill perform a self-diagnostic check.

9. Once all diagnostic checks have been passed, the ReliaPrep™ LV 32 HSM Instrument is readyfor operation.Notes:While the instrument can be controlled by a computer, an external computer is not requiredfor normal operation of the instrument. If the instrument fails any of the diagnostic checks, an error message will be displayed. Referto Section 4.F of this manual for more information or contact Promega Technical Services oryour authorized service representative for assistance.

3.B. Shutdown

Turn off the power switch on the front of the instrument, then turn off the switch on the back of thepower supply. Unplug the Power Supply from the electrical socket. Disconnect the ReliaPrep™ LV 32HSM Instrument from the Power Supply.

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4. Operating the ReliaPrep™ LV 32 HSM Instrument

4.A. Main Menu Navigation

After successful self-diagnostic check, the Main Menu is displayed.

Menu Options

1. Remote. This menu option allows the instrument to be controlled by an external computerconnected via RS-232 cable to the port on the back of the ReliaPrep™ LV 32 HSM Instrument.

2. ReliaPrep. This menu option allows semi-automated processing of blood samples using theReliaPrep™ Large Volume HT gDNA Isolation System (Cat.# A1751).

3. Manual. This menu option allows manual control of the ReliaPrep™ LV 32 HSM Instrumentfunctions without connecting it to an external computer.

4. Setup. This menu option offers diagnostic testing and demo modes. See Section 7.A for details.

Use the "Up" and "Down" buttons to move the cursor to the desired menu choice. Once the cursor ismoved to the desired position in the list, press the "ENTER" button to make the selection. At any timeduring the selection process, you may press the "ESC" button to return to the previous menu.

4.B. Sample Purification Using the ReliaPrep™ Large Volume HT gDNA Isolation System

The ReliaPrep™ LV 32 HSM Instrument is designed to isolate genomic DNA from human whole bloodsamples using the ReliaPrep™ Large Volume HT gDNA Isolation System. Please refer to the ReliaPrep™Large Volume HT gDNA Isolation System Technical Manual (#TM341) for detailed instructions regardinginstrument sample handling, setup and genomic DNA isolation. Before starting a run, please ensure thatyou have sufficient reagents available for the entire run.

The ReliaPrep™ LV 32 HSM Instrument is designed for use with potentially infectious materials. Usersshould use the appropriate protection (i.e., gloves, goggles, etc.) when handling potentially infectionssubstances. Users should adhere to their institutional guidelines for the handling and disposal of allinfectious materials. If you are processing less than 32 samples, we recommend placing empty 50mltubes in unused positions to reduce the chance of instrument contamination.

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4.B. Sample Purification Using the ReliaPrep™ Large Volume HT gDNA Isolation System(continued)

The ReliaPrep™ Large Volume HT gDNA Isolation System reagents contain potentially hazardouschemicals. Users should wear protective gloves or use other protective means when handling thereagents. Users should follow their institutional guidelines for disposal.

Starting a Purification Run

1. After the instrument has been set up and the diagnostic tests completed (Section 3.A) followthe instructions in Section 3.A to set up the instrument prior to the DNA isolation run.

2. Using the scroll “down” button, select option 2 “ReliaPrep” from the Main Menu.

3. Using the scroll buttons, choose the appropriate protocol (3, 6 or 10ml blood).

4. Follow the prompts on the screen to process samples or refer to the ReliaPrep™ Large VolumeHT gDNA Isolation System Technical Manual #TM341.

4.C. Remote Menu Option

The Remote menu option allows the ReliaPrep™ LV 32 HSM Instrument to be controlled by an externalcomputer through the RS-232 port. For fully automated sample processing, the ReliaPrep™ LV 32 HSMInstruments may be integrated with a robotic liquid handling workstation. In this configuration theinstruments are all connected to a computer and controlled by a driver in the workstation software. Fordetailed information on integrating the ReliaPrep™ LV 32 HSM Instrument with a liquid handlingworkstation, please contact [email protected].

4.D. ReliaPrep™ Menu Option

The ReliaPrep™ LV 32 HSM firmware contains preprogrammed methods that can be used to process 3,6 or 10ml of blood. The instrument provides processing instructions through the LCD screen. Pleaserefer to the ReliaPrep™ Large Volume HT gDNA Isolation System Technical Manual (#TM341) for detailedinstructions.

4.E. Manual Menu Option

The ReliaPrep™ 32 LV HSM Instrument can be operated manually, such that shaker (speed, directionand duration), temperature and magnet (on/off) are controlled independently.

This instrument is designed for use with ReliaPrep™ nucleic acid isolation reagents. Use with any otherreagents must be developed, optimized and validated by the user.


4.E. Manual Menu Option (continued)

When operating the ReliaPrep™ 32 LV HSM in manual mode, care must be taken to avoid liquid spillsand aerosols. When testing a new liquid or shaking speed, the optimal RPM setting should bedetermined by shaking at a minimum speed and increasing until the desired mixing is obtained. Table 1provides the maximum shaking speeds determined for two solutions, water and 50% ethanol.

Table 1. Maximum recommended shaking speeds for different amounts of liquid. When testing a newliquid/RPM combination, never exceed the recommended settings, and observe the liquid shaking in thetube to verify that the liquid/RPM combination does not result in spillage. Never exceed 35ml solutionin a processing tube.

The following parameters can be controlled:Parameter Available SettingsVelocity (rpm) 150–700*Shaking Time hh:mmPMPs Collect Time hh:mmTemperature ambient temperature up to 80°C.Direction CW, CCW

*Note: We do not reccommend operating the shaker below 150rpm.Select each parameter by pressing the scroll “Up” or “Down” keys, and press enter to start makingchanges. The value can be increased or decreased by pressing the scroll “Up” or “Down” keys. Press the“ESC” button after the settings are changed. Scroll to option 1 “Run” on the LCD screen, and press“ENTER” to start the run. The ReliaPrep™ LV 32 HSM Instrument will perform the heating and shakingbased on the set parameters. The PMP collection step will begin after the heating and shaking steps havecompleted.

4.F. Setup Menu Option

Setup displays the firmware version, performs diagnostic tests and allows access to the demo mode.

Firmware Version

Selecting “Firmware Version” identifies the currently loaded version of firmware on the ReliaPrep™ LV32 HSM Instrument. Press the Escape (ESC) button to return to the Setup menu option.

Maxiumum Recommended Shaking Speed with:

Volume Water 50% ethanol

35ml 550rpm 450rpm30ml 620rpm 550rpm25ml 680rpm 650rpm20ml 700rpm 680rpm15ml 700rpm 700rpm10ml 700rpm 700rpm


Diagnostic Test

The “Diagnostic Test” option verifies that the heater, shaker and magnets are performing within thespecifications for the instrument. The message "Diagnostic Testing Please Wait…" is displayed on theLCD screen. The Setup Menu will be displayed after the diagnostic testing is completed and no errors areidentified. An error message will be displayed if the diagnostic testing identifies a parameter outside ofthe acceptable range for the ReliaPrep™ LV 32 HSM Instrument. If this happens, one of the followingerrors will be displayed: “Shaker/Sensor Error”, “Magnet/Sensor Error” or “Heater/Sensor Error”. Turnoff the instrument, wait for 10 minutes and turn the instrument back on. If the error message continues tobe displayed then contact Promega Technical Services for additional assistance.

Reliability Test

The “Reliability Test” option is used only during manufacture and testing of the ReliaPrep™ LV 32 HSMInstrument and is not intended for general use.

ReliaPrep™ Demo

The ReliaPrep™ Demo includes all the steps of the ReliaPrep™ HT gDNA Isolation System, but theduration of steps is reduced for demonstration purposes, making it unsuitable for DNA isolation. Thisprogram is for demonstration purposes only and should not be used for DNA isolation.

5. ReliaPrep™ Instrument Cleaning and Maintenance

The ReliaPrep™ LV 32 HSM Instrument requires minimal maintenance. However, it is important toclean the instrument at regular intervals. Spilled samples or reagents should be cleaned up immediately.Most parts of the instrument have an anodized coating, which forms a durable, easily cleaned barrier.However, if liquid gets inside the instrument, it may damage the electronics.

Always turn off and unplug the ReliaPrep™ Instrument or the Power Supply before cleaning.

No additional maintenance is required for the ReliaPrep™ Instrument, Power Supply or accessories.The Instrument and Power Supply do not contain any user-serviceable parts. Removing the Instrumentor Power Supply casing will void the warranty.

General Care

Wipe up any spills immediately.

After each use, clean the instrument by wiping off the top and sides of the ReliaPrep™ Instrument andthe Tube Rack using a cloth dampened with deionized water or 70% ethanol. Do not use other solventsor abrasive cleaners because they can damage the instrument’s anodized coating.Note: Wear gloves. If the instrument is used with biohazardous materials, dispose of any cleaningmaterials used in accordance with your institutional guidelines.

Keep the cooling vents in the back of the machine clear of dust.

If the inside of the ReliaPrep™ Instrument becomes contaminated with samples or reagents, remove theTube Rack and Cover to gain access to the tube holders and the magnet bar. The cover can be removedby loosening the eight screws that attach the cover to the instrument (Figure 6). Once the cover is


5. ReliaPrep™ Instrument Cleaning and Maintenance (continued)

removed, wipe the tube holders and the magnet bar using a cloth dampened with deionized water.Wipe off the magnet bar and the tube holders using a cloth and foam- or cotton-tipped swabsdampened with 70% ethanol followed by a cloth and foam- or cotton-tipped swabs dampened with a1–2% bleach solution in deionized water. Follow immediately with a cloth and foam- or cotton-tippedswabs dampened with deionized water to remove any residual bleach from the instrument surfaces.Repeat the procedure as many times as required to effectively disinfect and decontaminate theinstrument.

• Keep the cooling vents in the back of the instrument and top of the Power Supply clear of dust.

• Do not remove the ReliaPrep™ LV 32 HSM Instrument case for cleaning. This will void the warranty.

• Do not use a spray bottle to soak equipment surfaces with large volumes of liquid.

• Never allow liquids to sit on equipment surfaces for extended periods of time.

Figure 6. Removing the cover for cleaning. Remove and properly discard any tubes from the Tube Rack, andremove the Tube Rack from the Instrument. Loosen and remove the eight screws that hold the cover on theinstrument, and remove the cover to gain access to the tube holders and magnet bar. Do not remove the instrumentcase. Removing the instrument case will void the warranty.

cover


6. Troubleshooting

If you have questions that are not covered in this troubleshooting section, contact Promega Technical Services. Seethe Promega web site, www.promega.com, to find your nearest Promega branch or distributor.

Symptoms Possible Causes and Comments

The instrument is making an One of the machine sensors might haveunusual, rapid clicking noise dust interfering with it. Contact Promegawhen it is turned on. or your local Promega representative for

assistance with sensor cleaning.

The LCD screen does not light up Check the Power Supply. If the Powerwhen the instrument is turned on. Supply switch is turned on, the switch

does not glow green, and you cannot hearthe Power Supply fans running:

•Check that the black Power Cord issecurely connected to the back of the PowerSupply.•Check that the black Power Cord issecurely plugged into a working electricaloutlet.•A 4-amp time-lag fuse is located next to thepower switch on the back of the PowerSupply. Check the fuse. If the fuse is blown,replace the fuse.

If you can hear the Power Supply fans running:•Check that the blue Power Cable is connectedbetween the back of the Instrument and the Power Supply.•Confirm that the power switch on the frontof the instrument and the power switch on theback of the Power Supply are both in the “on”position.•If the blue Power Cable is connected and theswitch is “on”, either a cable has becomedisconnected from the LCD screen, or the LCDscreen is broken or damaged. ContactPromega or your local Promega representativefor service.

“Heater error” message is Something is wrong with the electricaldisplayed. heating system. Contact Promega or your

local Promega representative for service.


6. Troubleshooting (continued)

Symptoms Possible Causes and Comments

The buttons do not work. The buttons are context specific. If a button consistently fails to work, please contact Promega or your authorized Promegarepresentative for service.

Poor-quality, low-yield or See the Technical Manual (#TM341)low-purity DNA obtained. supplied with your ReliaPrep™ Large

Volume Blood Genomic DNA IsolationSystem for more information.

Power failure occurs during an instrument run. The Main Menu will be shown on the LCDscreen after a power failure. Select the appropriate menu choice and advance through the steps using the “Down” keyto the point in the process where the powerfailure occurred. Restart at this point by pressingthe “Enter” key.

Magnetic particles are washed off The ReliaPrep™ Instrument is intended for useor not captured. only with Promega ReliaPrep™ paramagnetic

particles.

Check to make sure that themagnets are rotating 90 degrees duringcollection. You can look carefully in the sideof the instrument to observe the magnets turning.Alternatively, you can remove the cover (Figure 6) to observe them. Replacethe cover before running again. Do not removethe case because this will void the warranty.If the magnets are not rotating fully contactPromega or your authorized Promegarepresentative for service.

Inconsistent results. Instrument run without the tube rack or thecover. The HSM is designed to be used with thecover and tube rack in place; operation without these pieces may alter heating of the samples andadversely affect performance.


7. Appendix

7.A. Instrument Startup, Diagnostics and Errors

When instrument is powered on:

Each time the instrument is turned on, it will automatically perform a self-diagnostic test during whichthe platform, heating elements, and magnetic assemblies are initialized to check that the instrument isproperly calibrated. If the diagnostic testing is successful, the LCD screen will display, “DiagnosticCheck Successful”, and after a short pause the instrument will transition to the main menu screen.

An error message will display if the diagnostic testing identifies a parameter outside of the acceptablerange for the ReliaPrep™ LV 32 HSM Instrument. If a “Calibration Error” is detected, turn off theinstrument, wait for 10 minutes and turn the instrument back on. If the error persists, contact Promegatechnical services for additional assistance.

During operation, the instrument will automatically do periodic self-diagnostic tests to verify that theplatform, heating elements and magnetic assemblies are within calibration. If the instrument detects thatany of these parts are not within calibration during a run, the method will pause and display“Calibration Error”. If a “Calibration Error” occurs during run, contact Promega Technical Services.

7.B. Updating Firmware

As new purification kits and applications become available, new versions of firmware may be required.The firmware version installed on your instrument can be verified either by accessing the FirmwareVersion option from the Setup Menu (see Section 4.F) or by turning the machine off and then on again.

Firmware is updated using the Reliaprep.exe program that will be supplied with any firmware updatesand the RS-232 port on the back of the instrument. Alternatively, the instrument can be connected to aUSB port on the computer using the included USB-to-serial converter cable. Firmware can be updatedfrom any computer running Microsoft Windows® 95 or higher with an available serial port or by usingthe included USB-to-Serial converter cable.

1. Plug the provided RS-232 Cable into the back of the instrument and into a serial port on yourcomputer. Note the address of the COM port that is being used.

2. Obtain the firmware upgrade program from Promega or your local Promega representative.Save the file to your hard drive. Within the Reliaprep.exe software, first select the Com portto which the shaker is connected, and then select “connect”. Select “Upgrade firmware” tostart the firmware upgrade process. As part of this process you will browse to the savedlocation of the firmware upgrade that you have downloaded. Once you have selected thefirmware upgrade program to be used for the upgrade, select “upgrade”. Upgrading thefirmware may take a few minutes. After completing the upgrade process, a message will bedisplayed indicating that the firmware has been upgraded successfully. At this point you canquit the Reliaprep.exe program.

Please contact Promega or your local Promega representative for assistance if you encounter anyproblems during the firmware upgrade process.


7.C. Instrument Disposal

Contact your local Promega Representative for disposal of the instrument. Please follow your institutionalguidelines to handle the disposal of accessories.

7.D. Returning the ReliaPrep™ LV 32 HSM Instrument and Accessories

The ReliaPrep™ LV 32 HSM Instrument is designed to provide years of consistent performance with littlemaintenance. If a problem arises with your instrument, please contact Promega or your local Promegarepresentative for support. If further action is required, repair options will be presented and a returnauthorization assigned if necessary. Promega is not responsible for instrumentation returned without anauthorization number. When you ship the instrument for service, please remember to:

1. Obtain a return authorization from Promega.

2. Decontaminate the instrument (see Section 7.E for Decontamination Instructions).

3. Affix a signed and dated Certificate of Decontamination to the outside of the package in which theinstrument is returned (see Section 7.E). Failure to complete and attach the Certificate ofDecontamination will result in a decontamination charge.

4. Use the original packaging to ensure that no damage will occur to the equipment during shipping.Any damage will incur additional charges. Notes:If any of the original packaging is lost or damaged, contact Promega or your local Promegarepresentative for replacement packaging.The original packaging includes the shipment pallet that the instrument was shipped on originally.Please use the same pallet for the return shipment.

5. Repack the equipment according to the following instructions.

Preparing the ReliaPrep™ LV 32 HSM Instrument and Power Supply prior to repacking:

1. Ensure that the tubes are removed from the instrument platform.

2. Ensure that the Power Supply is turned off and not plugged in.

3. Ensure that the ReliaPrep™ Instrument is turned off.

4. Make sure that the instrument is disconnected from the Power Supply.

!


Repacking the instrument:

1. Place the instrument back into the plastic bag.

2. Place the protective cardboard pieces over the ReliaPrep™ LV 32 HSMInstrument and over the Power Supply.

3. Repack the ReliaPrep™ LV 32 HSM Instrument accessories.

a. Rewrap the Tube Rack, Tube Rack Stand and Power Supply in bubblewrap and place in the appropriate location in the shipping container.

b. Place the RS-232 Cable, the Power Cable and the Power Cord intoresealable bags, and place the bags into the accessories box. Place the accessories box into the shipping container.

6. Affix the Certificate of Decontamination on the outside of the shipping box. Write the returnauthorization number provided to you by Promega or your local Promega representative on theoutside of the shipping box. Seal the box securely and send back on the original pallet provided.


7.E. Certificate of Decontamination

Disinfection and decontamination are required prior to shipping the instrument and instrumentaccessories for repair. Instruments returned must be accompanied by a signed and dated Certificate ofDecontamination attached to the outside packaging of the instrument.

To disinfect and decontaminate: Wipe off the outside surfaces using a cloth dampened with 70% ethanolthen a cloth dampened with a 1–2% bleach solution in deionized water. Follow immediately with a clothdampened with deionized water to remove any residual bleach from the instrument surfaces. Removethe Tube Rack (Figure 1) and the cover (Figure 5) to expose the Magnet Bar and the Tube Holders. Wipeoff the Magnet Bar and the Tube Holders using a cloth and foam- or cotton-tipped swabs dampenedwith 70% ethanol followed by a cloth and foam- or cotton-tipped swabs dampened with a 1–2% bleachsolution in deionized water. Follow immediately with a cloth and foam- or cotton-tipped swabsdampened with deionized water to remove any residual bleach from the instrument surfaces. Repeat theprocedure as many times as required to effectively disinfect and decontaminate the instrument.

Failure to confirm disinfection and decontamination will result in decontamination charges before theinstrument will be serviced.Select either (A) or (B):

A. I confirm that the returned items have not been contaminated by body fluids or by toxic,carcinogenic, radioactive, or other hazardous materials.

B. I confirm that the returned items have been decontaminated and can be handled withoutexposing personnel to health hazards.

Circle the type of material used in the instrument: Chemical Biological Radioactive**

Briefly describe the decontamination procedure performed:

Date:___________________________Place:________________________________________________________________________________________

Signature:

Name (block capital letters):

** The signature of a Radiation Safety Officer is also required if the instrument was used withradioactive materials.

This instrument is certified by the undersigned to be free of radioactive contamination.

Date:________________________Place:________________________________________________________________________________________

Signature:

Name (block capital letters):


7.F. Warranty Information

Limited Warranty and Service Guidelines (Pending Warranty and Service Contract Information)

Promega warrants to the original purchaser that the Promega ReliaPrep™ LV 32 HSM Instrument willbe free from defects in materials and workmanship for a period of one year from the date of delivery.Promega agrees, as its sole responsibility under this limited warranty and upon prompt notice of adefect, to repair or replace (at Promega’s discretion) any instrument discovered to be defective withinthe warranty period. Expendable items are not covered by this warranty. This warranty does not includerepair or replacement necessitated by accident, neglect, misuse, unauthorized repair or modification ofthe instrument. The instrument may not be returned without a proper Return Authorization Numberfrom Promega, as described below.

This warranty and the remedies set forth herein are exclusive and in lieu of all other express or impliedwarranties (including implied warranties of merchantability, fitness for a particular purpose andnoninfringement), and no other warranties shall be binding upon Promega. In no event shall Promegabe liable for any special, incidental or consequential damages resulting from the use or malfunction ofthis instrument or the system with which it is used.

To obtain service during the warranty period, please take the following steps:

1. Write or call the company that sold you the instrument and describe as precisely as possiblethe nature of the problem.

2. Carry out minor adjustments or tests as suggested by your technical contact.

3. If the instrument is still not functioning properly and needs to be returned to Promega forservice, YOU MUST OBTAIN A PROMEGA RETURN AUTHORIZATION NUMBER prior toshipping the instrument back.

4. Before returning the equipment, you will be responsible for cleaning it and providing aCertificate of Decontamination to Promega in accordance with instructions.

5. After obtaining a Return Authorization Number and signing the Certificate ofDecontamination, pack the instrument carefully (damage incurred in shipping due toimproper packaging is not Promega’s responsibility), write the Return Authorization Numberon the outside of the package and ship it to the address provided by your technical contact.

6. Shipping to and from Promega will be paid by Promega pursuant to directions to beprovided. The instrument will be repaired free of charge for all customers within theirwarranty period.

7. Under no circumstance can equipment be returned without proper authorization. Thisauthorization is needed to ensure that the problem is not a minor problem that can be easilyhandled in your laboratory and to determine the nature of the problem so that repairs can behandled appropriately.

Out of Warranty Service

Contact Promega or your local Promega representative. We will be happy to assist you by telephone atno charge. Repair service, if needed, will be billed at a flat rate to be agreed upon in advance. Yourinvoice will include shipping.


7.G. Related Products, Warranties and Service Agreements

DNA Purification Kits

Product Quantity Cat.#ReliaPrep™ Large Volume HT gDNA Isolation System 96 × 10ml to 960 × 1ml preps A1751

Replacement Components

Product Quantity Cat.#ReliaPrep™ LV 32 HSM Instrument Cover 1 each A1712ReliaPrep™ Tube Rack 1 each A1713ReliaPrep™ Tube Rack Stand 1 each A1714

Warranties

The Standard Warranty, included in the system price, covers all parts, labor and shipping to and fromour service facility as well as a temporary replacement upon request. The temporary replacement will beshipped via standard ground shipment and will arrive in 5 to 7 working days. If you no longer haveyour instrument shipping carton, we will provide you with a box for shipment of the instrument back toour service technicians. We will repair it and return it to you performing to original factoryspecifications.

Service Agreement Options

After the warranty period is over, you can continue to receive the same comprehensive service andsupport from Promega you did when your system was under warranty. The Standard ServiceAgreement covers all parts, labor and shipping to and from our depot repair location as well as a loanerinstrument upon request. If your instrument needs repair, we will provide a box for shipment of theinstrument back to our service facility. We will repair it and return it performing to original factoryspecifications. The term is one year, and it is renewable.

Product Quantity Cat.#ReliaPrep™ LV 32 HSM Instrument Standard Service Agreement 1 each SA3070

© 2010–2013 Promega Corporation. All Rights Reserved.ReliaPrep is a trademark of Promega Corporation.Windows is a registered trademark of Microsoft Corporation.Products may be covered by pending or issued patents or may have certain limitations. Please visit our Web site for more information.All prices and specifications are subject to change without prior notice.Product claims are subject to change. Please contact Promega Technical Services or access the Promega online catalog for the most up-to-date information on Promega products.

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