Report AAFC15-068T-200 Alion Herbicide on Blueberries - Feb...

EFFICACY/CROP TOLERANCE PERFORMANCE FORM Page 1 of 69 Tolerance Study AAFC15-068T-200 Indaziflam / Lowbush Blueberry Project title: Crop Tolerance of Lowbush Blueberry to Alion Herbicide

(indaziflam) Over Two Years. Sprouting and Harvest Years.

Final Report Project Lead: Greg O’Neill Agriculture and Agri-Food Canada Minor Use Pesticide Program Building 57, Central Experimental Farm 960 Carling Avenue Ottawa, Ontario K1A 0C6 Phone: (613) 694-2454 Fax: (613) 759-1400 Email: [email protected] Investigator: Jim Jotcham, Marbicon Inc. 4287 Brooklyn St., Berwick, NS, B0P 1E0 Tel. (902) 538-7101 Email: [email protected] Introduction Alion Herbicide (indaziflam) is a Group 29 selective preemergence herbicide for season long control of important broadleaf and grass weeds in apples, pears, peaches, nectarines, plums, cherries (sweet/sour), apricots, grapes and tree nuts grown in Eastern Canada and British Columbia. ALION Herbicide provides residual control of susceptible grass and broadleaf weeds when applied preemergence to the soil. Alion Herbicide may only be applied once per calendar year. The mode of action of Alion Herbicide is by inhibiting cellulose biosynthesis (cell wall synthesis) in the meristem. It is a member of the Alkylazine chemical family. The purpose of this study was to test the tolerance to dormant applications of Alion Herbicide in the production of blueberries, with a first application in the sprout year, and a second application in the fruiting year.

EFFICACY/CROP TOLERANCE PERFORMANCE FORM Page 2 of 69 Tolerance Study AAFC15-068T-200 Indaziflam / Lowbush Blueberry Materials & Methods Trial location: Service Road PIC 104-01 (Exit 18A, Hwy 104) Mount Thom, Pictou County, Nova Scotia N 45o 29’ 19” W 62o 59’ 27” The nearest weather station was Upper Stewiacke RCS Nova Scotia, about 29.5 km south of site, but it was missing weather data, so 2015-2016 information was taken from Debert, about 39 km west of the test site Target Pests: Labelled Weeds. Experimental Pesticide : Alion Herbicide (indaziflam, 200 g a.i./L, Group 29) Manufacturer: Bayer CropScience Canada CAS No: 950782-86-2 PCP No: 30451 Batch/Lot No: NT57EX4205 (container 610082) Standard: Velpar DF CU Herbicide (hexazinone, 750 g a.i./kg, Group 5) CAS No: 51235-04-2 PCP No: 25225 Batch/Lot No: not available Crop: Lowbush blueberry, Vaccinium angustifolium Seed Source: n/a - established perennial crop Planting Date: n/a - established perennial crop (estimated 40+ years) Method of planting: n/a - established perennial crop Emergence date: Sprouts had emerged by May 25 2015 (see Figure 3) Transplant date: n/a - established perennial crop Flowering date: n/a in sprout year; early-to mid June 2015 in adjacent crop fields Plot size & Experimental design: 9 m2 plot (3 m x 3 m) RCBD with 4 replicates. See Figure 3 for plot diagram. Row spacing: n/a - clonal growth Plant spacing: n/a - clonal growth

EFFICACY/CROP TOLERANCE PERFORMANCE FORM Page 3 of 69 Tolerance Study AAFC15-068T-200 Indaziflam / Lowbush Blueberry Soil type: Sandy Loam Soil pH: 5.1 % Organic matter: 9.5 % Soil CEC: 18.6 meq/100g Soil Texture: 56.0 % Sand, 37.4 % Silt, 6.6 % Clay Table 1. Maintenance chemicals/pesticides applied:

Date Product PCP # Formulation Rate a.i./ha 30 Apr 12 Mission 418 EC 28016 Propiconazole 418 g/L 125 g 7 May 12 Jade Fungicide 24030 Propiconazole 250 g/L 125 g 7 Jun 12 Switch 62.5 WG 28189 Cyprodinil 37.5%

Fludioxonil 25.0% 290 g 194 g

29 Jun 12 Pristine WG 27985 Boscalid 25.2% Pyraclostrobin 12.8%

328 g 166 g

26 Jul 12 Concept Insecticide 29611 Imidacloprid 75 g/L Deltamethrin 10 g/L

42 g 1.5 kg

26 May 13 Velpar DF 25225 Hexazinone 750 g/kg 1.5 kg 29 May 13 Sinbar WDG 30082 Terbacil 80% 1.2 kg 30 May 13 Fertilizer n/a 16.5 - 34.5 - 4.9 n/a 25 Jul 13 Bravo 500 15723 Chlorothalonil 500 g/L 2.7 kg 25 Jul 13 Proline 480 SC 28359 Prothioconazole

480 g/L 151 g

2014 NONE1 n/a n/a n/a 2015 NONE1 n/a n/a n/a 2016 NONE1 n/a n/a n/a

1. There were no maintenance chemicals applied in 2014 - 2016 to the trial area. Cultural practices: Mowed in fall 2012 (after cropping) None in 2013 (sprout year) Mowed in fall 2014 (after cropping) None in 2015 (sprout year) Mowed in fall 2016 (after cropping) Inoculation: n/a - a tolerance trial only Irrigation: None applied.

EFFICACY/CROP TOLERANCE PERFORMANCE FORM Page 4 of 69 Tolerance Study AAFC15-068T-200 Indaziflam / Lowbush Blueberry Table 2. Application Data

Date

TRT #

Treatment1

(rate of product/ha)

Rate product per ha

Application

Type

Volume 2015-05-14 1 UTC N/A N/A N/A 2015-05-14 2 Alion 375 mL broadcast 400 L/ha 2015-05-14 3 Alion 750 mL broadcast 400 L/ha 2015-05-14 4 Velpar DF 2.22 kg2 broadcast 350 L/ha 2016-4-22 1 UTC N/A N/A N/A 2016-4-22 2 Alion 375 mL broadcast 400 L/ha 2016-4-22 3 Alion 750 mL broadcast 400 L/ha 2016-4-22 4 Velpar DF 1.3 kg2 broadcast 350 L/ha

1. No adjuvants were added to the spray mixes. 2. Velpar DF: 2.22 kg/ha is within the label range for the sprouting year. 1.3 kg/ha is the label rate for the cropping year Application equipment (2015 and 2016): Backpack CO2 sprayer, 3-m boom (6 nozzles @ 50 cm spacing). TeeJet flat fan 80015 nozzles with a 50-mesh strainer. Boom held at approximately 50 cm from the ground during application in 2015. Boom held at approximately 50 cm from the canopy during application in 2016. Spray record: Application #: 1 Date: May 14 2015, 12:05 pm - 1:00 pm Crop growth stage: dormant Soil moisture: moist Air Temp: 16 o C Soil Temp: 5 o C Relative humidity: 30 % Wind speed/direction: 4.9 kph SW Cloud Cover %: none 1st rainfall after trt.: 0.3 mm, May 16 Crop stress: none Crop height: 0.0 m Dew: none Carrier pH: 7 Product placement: broadcast Pest (type & population): n/a Average plants/ha: n/a - clonal growth

EFFICACY/CROP TOLERANCE PERFORMANCE FORM Page 5 of 69 Tolerance Study AAFC15-068T-200 Indaziflam / Lowbush Blueberry Application #: 2 Date: April 22 2016, 7:30 pm - 8:00 pm Crop growth stage: prior to bud break Soil moisture: dry Air Temp: 11.0 o C Soil Temp: 10 o C Relative humidity: 51 % Wind speed/direction: 0 - 2.5 kph N Cloud Cover 5 %: 1st rainfall after trt.: 8.1 mm, April 23* Crop stress: None Crop height: 15-30 cm Dew: None Carrier pH: 6.5 Product placement: broadcast Pest (type & population): n/a Average plants/ha: n/a - clonal growth * 0.8 mm rainfall recorded for April 22 2016 may have been on the same day; time unknown.

EFFICACY/CROP TOLERANCE PERFORMANCE FORM Page 6 of 69 Tolerance Study AAFC15-068T-200 Indaziflam / Lowbush Blueberry Figure 1. Plot Map: (to access road) North

Velpar

UTC

UTC

Alion

375 mL

Alion

375 mL

Alion

750 mL

Alion

750 mL

Velpar

Alion

750 mL

Alion

375 mL

Velpar

Alion

750 mL

UTC

Velpar

Alion

375 mL

UTC

Plot design (above) showing 4 treatments in a random block design. Each plot was 3 m x 3 m (0.0009 ha). There was a 0.5m untreated buffer between plot rows, to avoid start/end effects. In addition, all sampling was restricted to the centre of the plot to avoid side edge effects and/or drift contamination. Spraying was done from the NE to the SW (moving away from the road). Plot corners were marked with wire flags and/or wood survey stakes.

EFFICACY/CROP TOLERANCE PERFORMANCE FORM Page 7 of 69 Tolerance Study AAFC15-068T-200 Indaziflam / Lowbush Blueberry

Figure 2. General photograph of the research site looking southeast. May 14, 2015. Snow was still present near the plots on the date of application.

Figure 3. Crop growth stage on May 25 2015, 11 days after herbicide application. Shoot buds were just coming up to the surface.

EFFICACY/CROP TOLERANCE PERFORMANCE FORM Page 8 of 69 Tolerance Study AAFC15-068T-200 Indaziflam / Lowbush Blueberry Table 4. Rainfall records (-7 days + 10 days) for herbicide application 1 (2015). Weather station = Debert (Environment Canada) Latitude 45o 25’ 00' N; Longitude 63o 25’ 00" W; Elevation 38.1 m Approximately 39 km west of the trial site. Complete daily weather records for April 2015 to September 2015 are in Appendix 1.

Day Date Rain mm Comments -7 2015-05-07 M Missing rainfall data -6 2015-05-08 0.0 -5 2015-05-09 0.5 -4 2015-05-10 1.5 -3 2015-05-11 8.4 -2 2015-05-12 8.3 -1 2015-05-13 0.7 0 2015-05-14 0.3 Application May 14 @ 12:05pm-1:00pm 1 2015-05-15 0.0 2 2015-05-16 0.3 1st rain event 3 2015-05-17 0.7 4 2015-05-18 0.6 5 2015-05-19 0.5 6 2015-05-20 M Missing rainfall data (Halifax = 0.2 mm) 7 2015-05-21 0.0 8 2015-05-22 5.0 9 2015-05-23 3.8 10 2015-05-24 0.9

EFFICACY/CROP TOLERANCE PERFORMANCE FORM Page 9 of 69 Tolerance Study AAFC15-068T-200 Indaziflam / Lowbush Blueberry Table 5. Rainfall records (-7 days + 10 days) for application 2 (2016). Weather station = Debert (Environment Canada) Latitude 45o 25’ 00' N; Longitude 63o 25’ 00" W; Elevation 38.1 m Approximately 39 km west of the trial site. Complete daily weather records for April 2015 to August 2016 are in Appendix 1.

Day Date Rain mm Comments -7 2016-04-15 0.4 -6 2016-04-16 0.0 -5 2016-04-17 1.0 -4 2016-04-18 0.0 -3 2016-04-19 0.2 -2 2016-04-20 0.0 -1 2016-04-21 0.3 0 2016-04-22 0.8 Application 2

Halifax Intl A recorded no rain until the afternoon on 2016-04-23.

1 2016-04-23 8.1 1st rain event 2 2016-04-24 0.4 3 2016-04-25 0.5 4 2016-04-26 1.1 5 2016-04-27 Missing (0.0 mm recorded at Halifax Intl A) 6 2016-04-28 0.0 7 2016-04-29 0.2 8 2016-04-30 0.0 9 2016-05-01 0.6 10 2616-15-02 6.5

NOTES: The nearest weather station to the test site, Stewiacke RCS, was missing some weeks of weather data in May and June in 2015, so test site data for both years was collected from Debert, which was the next nearest Environment Canada station. Halifax Intl A is the nearest weather station to record detailed hourly weather, about 79 km south of the test site. Irrigation to test site: None in 2015 or 2016.

EFFICACY/CROP TOLERANCE PERFORMANCE FORM Page 10 of 69 Tolerance Study AAFC15-068T-200 Indaziflam / Lowbush Blueberry Materials & Methods: Assessments: Although some emergence was seen on May 15 2015, no assessments were done until the shoots had more completely emerged, on June 24 2015. A field check was done on May 25 and June 15 2015. By mid-June, adjacent crop fields were starting to bloom. The study trial field had visible shoots (Figure 4, below) and the site was thus deemed ready for quantitative assessments on June 24 2015. A transect system to count stem height was employed as per protocol. The first 2016 height assessment was on July 5 2016.

Figure 4. Typical shoot emergence on June 15 2015. Twenty-five stems per plot were measured for height, from the south corner of the plot to the north corner, excluding the end points (to remove edge effects). So the 3 m x 3 m plot allowed a central 2.5 m transect from the west to the east corner with a stem height value taken every 10 cm (nearest stem to the point). Stem presence/absence under the point was also recorded to give a measure of stem frequency. With 4 replicate plots, this provided 100 data points per treatment, with both the nearest stem height and the actual stem frequency being recorded. Stem height and frequency data were collected on June 24/2015, July 7/2015, July 23/2015, Jul 5/2016, and July 29/2016. For foliage cover assessment, four random 25-cm square (625 cm2) quadrats per plot were used to estimate the percent cover. The cover assessments were on August 21 2015 and August 19 2016. The assessment in 2016 coincided with the time of crop harvest in adjacent fields. On August 19 2016, yield samples of all marketable berries were taken from 625- cm2 square quadrats and weighed (grams). The total area harvested per treatment was one m2.

EFFICACY/CROP TOLERANCE PERFORMANCE FORM Page 11 of 69 Tolerance Study AAFC15-068T-200 Indaziflam / Lowbush Blueberry Results: Plots were set up on May 14, 2015 and the first application of treatments in the sprouting year was applied later that same day. Observations made on May 21 revealed that shoots were just breaking through the surface. By June 15, the tallest shoots were approaching 10 cm height, so the first quantitative methods could be initiated. Application in 2016 was somewhat earlier in the year than in 2015 because of a warmer spring. The application in the fruiting year was April 22, 2016. Stem height data for 2015 were collected on June 24, July 7, and July 23. 2016 data were collected on July 5 and July 29. All raw data are presented in Appendix 2. A summary of the stem height means and associated standard error is presented in Table 6. Initially. the plots treated with Alion at 750 mL/ha were significantly shorter from most of the other treatments at the 0.05 level. By July 2015, there were no significant differences between any treatments and the untreated. There were no significant differences in stem height in 2016. Table 6: Summary of stem heights (cm); 100 stems (25 stems in each of 4 plots). Date

Parameter

Untreated Control

Alion 375 mL/ha

Alion 750 750 mL/ha

Velpar DF

June 24/15 Year 1

Mean 6.56 b 6.84 b 5.5 a 6.13 ab Std. Error 0.21 0.38 0.22 0.22

July 07/15 Year 1

Mean 12.17 11.85 11.05 12.12 Std. Error 0.37 0.34 0.29 0.36

July 23/15 Year 1

Mean 18.77 17.62 17.42 18.22 Std. Error 0.44 0.42 0.39 0.41

July 05/16 Year 2

Mean 25.26 25.26 25.98 24.75 Std. Error 0.55 0.52 0.54 0.46

July 29/16 Year 2

Mean 26.70 25.58 25.76 25.77 Std. Error 0.57 0.57 0.45 0.51

Different letters after the mean indicate significant differences at the 0.05 level, based on Tukey's HSD test. Stem frequency (presence/absence) data for June 24, July 7, and July 23, 2015, plus July 5 and July 29 2016 are summarized in Table 7. All raw data are presented in Appendix 2. The Velpar plots had significantly more stems than the other treatments on June 24 2015, but otherwise there were no significant differences among treatments on subsequent assessments.

EFFICACY/CROP TOLERANCE PERFORMANCE FORM Page 12 of 69 Tolerance Study AAFC15-068T-200 Indaziflam / Lowbush Blueberry Table 7: Summary of percent stem frequency; 100 assessments (25 assessments in each of 4 plots). Date

Parameter

Untreated Control

Alion 375 mL/ha

Alion 750 750 mL/ha

Velpar DF

June 24/15 Year 1

Mean 0.45 a 0.54 ab 0.44 a 0.65 b Std. Error 0.05 0.05 0.05 0.05

July 07/15 Year 1

Mean 0.77 0.77 0.71 0.84 Std. Error 0.04 0.04 0.05 0.04

July 23/15 Year 1

Mean 0.90 0.96 0.94 0.95 Std. Error 0.03 0.02 0.02 0.02

July 05/16 Year 2

Mean 0.97 0.96 0.95 0.99 Std. Error 0.02 0.02 0.02 0.01

July 29/16 Year 2

Mean 0.83 0.83 0.80 0.90 Std. Error 0.04 0.04 0.04 0.03

Different letters after the mean indicate significant differences at the 0.05 level, based on Tukey's HSD test. The first annual stem density assessment (as measured by percent cover, using 4 quadrats per plot with each quadrat measuring 625 cm2) was on August 21, 2015, during the time of crop harvest in adjacent fields. Table 8 summarizes the percent cover results. The 2016 values were taken on August 19. In 2015, the Velpar treatment exhibited significantly greater cover values at the 0.05 level. In 2016, there were no significant differences in percent cover among all treatments. Table 8: Summary of percent cover; 4 quadrats in each of 4 plots per treatment. Date

Statistic

Untreated Control

Alion 375 mL/ha

Alion 750 750 mL/ha

Velpar DF

August 21/15 Year 1

Mean 90.31% a 90.31% a 92.81% ab 96.25% b Std. Error 0.53 0.47 1.03 0.44

August 19/16 Year 2

Mean 94.38% 95.00% 94.69% 94.69% Std. Error 0.43 0.00 0.31 0.31

Different letters after the mean indicate significant differences to any treatment at the 0.05 level using Tukey's HSD test. Yield data (grams fresh weight) were taken from 4 quadrats per plot with each quadrat measuring 625 cm2 (25 cm x 25 cm)) on August 19, 2016, at the same time as commercial crop harvest in adjacent fields. Only marketable fruit were collected. Table 9 summarizes the yield data. There were no significant differences among any treatments.

EFFICACY/CROP TOLERANCE PERFORMANCE FORM Page 13 of 69 Tolerance Study AAFC15-068T-200 Indaziflam / Lowbush Blueberry Table 9: Summary of Yield data (grams) for each of 4 quadrats in each of 4 plots per treatment. Date

Statistic

Untreated Control

Alion 375 mL/ha

Alion 750 750 mL/ha

Velpar DF

August 19/16 Year 2

Mean 24.88 35.56 30.13 27.13 Std. Error 3.19 3.05 3.51 3.53

Yield Kg/ha * 3980 5370 4820 4340 * 1 g/cm2 = 100,000 kg/ha Since there were no signs of phytotoxicity (such as deformations or discolouring) on any assessment date during both 2015 and 2016, no tabular data are presented. Discussion This was a two-year study, since lowbush blueberries are managed as a biannual crop, with the standing crop mowed to the ground in the fall after harvest to encourage sprout development in the following year. Fruit then develop on the second year growth, which in this trial, was 2016. Herbicides are known to increase shoot height in lowbush blueberries (Eaton, L. J. 1994. Long-term effects of herbicide and fertilizers on lowbush blueberry growth and production". Can J. Plant Sci. 74: 341-345). Alion at both 375 mL/ha and at 750 mL/ha did not have this effect. The Velpar plots had a higher stem height only on June 24 2015, and there were no significant height differences subsequently in either 2015 or 2016. In both 2015 and 2016, the first application of Alion herbicide was applied to a dormant field, before any shoot development. Subsequent monitoring showed no significant differences in stem height of the Alion-treated lowbush blueberries compared to the untreated at all assessments. There were no other signs of phytotoxicity in response to the any rate of Alion , such as leaf size, shape or colour. There were no significant differences in yield among any treatments and the untreated. Lowbush blueberries are tolerant to sequential Alion herbicide applications when applied once a year during dormancy.


Appendix 1. Weather data (Environment Canada) Note: Debert (about 39 km to the west) was the closest Environment Canada station with both complete weather data for 2015 and the 30-year mean (1981-2010) posted on the internet. This station was used for the entire trial, 2015-2016.



Debert, Nova Scotia Environment Canada 2015 Source: http://www.climate.weatheroffice.ec.gc.ca/climateData/dailydata_e.html

Max Min Mean TotalTemp Temp Temp Prcip

April oC oC oC mm1-Apr-15 M M M 0.02-Apr-15 M M M M

3-Apr-15 7.7 -3.7 2.0 0.94-Apr-15 2.4 -4.8 -1.2 14.35-Apr-15 -0.3 -15.9 -8.1 2.36-Apr-15 -2.1 -19.8 -11.0 1.47-Apr-15 3.4 -7.7 -2.2 2.98-Apr-15 0.5 -11.1 -5.3 0.29-Apr-15 4.2 -8.2 -2.0 0.0

10-Apr-15 5.3 -8.1 -1.4 5.711-Apr-15 6.6 1.0 3.8 0.512-Apr-15 7.5 -4.4 1.6 0.013-Apr-15 13.0 -6.1 3.5 0.214-Apr-15 9.4 -4.4 2.5 2.215-Apr-15 8.4 -6.1 1.2 0.416-Apr-15 5.6 -8.9 -1.7 0.017-Apr-15 8.7 -6.4 1.2 0.018-Apr-15 5.1 -3.8 0.7 0.219-Apr-15 5.5 -4.5 0.5 0.020-Apr-15 11.3 -6.8 2.3 0.021-Apr-15 6.3 0.6 3.5 23.022-Apr-15 8.9 2.3 5.6 12.923-Apr-15 7.2 1.5 4.4 11.824-Apr-15 7.3 0.2 3.8 0.925-Apr-15 8.6 -1.5 3.6 0.026-Apr-15 7.0 -1.9 2.6 0.027-Apr-15 4.6 0.4 2.5 9.628-Apr-15 3.2 0.4 1.8 4.529-Apr-15 6.6 0.8 3.7 0.030-Apr-15 5.1 0.9 3.0 0.2

Sum 94.1*Avg 6.0* -4.7 0.8*

* M = missing data

The above information was copied from the Environment Canada web site.




May oC oC oC mm1-May-15 8.4 1.1 4.8 0.02-May-15 14.0 -0.9 6.6 0.33-May-15 14.5 -2.0 6.3 0.34-May-15 19.6 -2.1 8.8 0.75-May-15 19.9 1.6 10.8 1.76-May-15 17.0 1.0 9.0 0.07-May-15 M M M M

8-May-15 10.8 -3.3 3.8 0.09-May-15 16.1 -3.9 6.1 0.5

10-May-15 15.9 7.3 11.6 1.511-May-15 13.6 6.1 9.9 8.412-May-15 15.9 4.8 10.4 8.313-May-15 10.2 -1.9 4.2 0.714-May-15 15.6 -3.3 6.2 0.315-May-15 21.3 -0.8 10.3 0.016-May-15 25.1 1.8 13.5 0.317-May-15 15.1 3.3 9.2 0.718-May-15 21.7 1.4 11.6 0.619-May-15 20.5 1.8 11.2 0.520-May-15 14.6 5.6 10.1 M

21-May-15 18.3 2.3 10.3 0.022-May-15 10.1 1.0 5.6 5.023-May-15 9.0 -0.9 4.1 3.824-May-15 18.8 -2.3 8.3 0.925-May-15 24.7 3.2 14.0 0.526-May-15 19.5 10.7 15.1 9.327-May-15 27.7 11.1 19.4 0.328-May-15 22.2 12.6 17.4 0.029-May-15 24.4 11.5 18.0 0.030-May-15 23.7 11.7 17.7 0.031-May-15 18.5 7.7 13.1 31.4

Sum 76.0*Avg 17.6* 2.9* 10.3*

* M = missing data




Max Min Mean Total Temp Temp Temp Prcip

June oC oC oC mm 1-Jun-15 10.1 6.0 8.1 1.5 2-Jun-15 6.5 5.2 5.9 19.3 3-Jun-15 12.7 6.1 9.4 0.4 4-Jun-15 18.7 4.0 11.4 0.0 5-Jun-15 20.1 2.5 11.3 0.9 6-Jun-15 12.9 2.4 7.7 15.3 7-Jun-15 16.1 0.5 8.3 0.0 8-Jun-15 21.5 0.5 11.0 0.5 9-Jun-15 24.5 12.5 18.5 0.0

10-Jun-15 20.4 12.7 16.6 2.5 11-Jun-15 23.9 10.6 17.3 0.0 12-Jun-15 23.8 10.1 17.0 0.0 13-Jun-15 22.1 9.8 16.0 26.2 14-Jun-15 14.6 2.2 8.4 M

15-Jun-15 20.9 1.7 11.3 M

16-Jun-15 18.8 4.9 11.9 8.5 17-Jun-15 20.4 6.7 13.6 1.8 18-Jun-15 24.1 6.7 15.4 0.3 19-Jun-15 14.6 6.9 10.8 13.4 20-Jun-15 20.8 5.4 13.1 0.3 21-Jun-15 18.1 4.7 11.4 35.1 22-Jun-15 15.2 10.6 12.9 5.8 23-Jun-15 19.6 10.1 14.9 11.0 24-Jun-15 20.1 8.9 14.5 13.3 25-Jun-15 20.6 7.5 14.1 1.0 26-Jun-15 17.8 5.9 11.9 0.3 27-Jun-15 19.6 6.2 12.9 0.9 28-Jun-15 16.0 5.8 10.9 12.6 29-Jun-15 18.1 9.7 13.9 1.1 30-Jun-15 22.7 8.8 15.8 0.0 Sum 172.0* Avg 18.5 6.5 12.5

* M = missing data





July oC oC oC mm 1-Jul-15 26.5 12.0 19.3 0.3 2-Jul-15 25.6 11.1 18.4 0.0 3-Jul-15 22.7 10.2 16.5 1.0 4-Jul-15 26.5 6.8 16.7 0.2 5-Jul-15 22.0 12.7 17.4 M

6-Jul-15 26.3 10.5 18.4 M

7-Jul-15 28.1 11.0 19.6 M

8-Jul-15 28.4 13.9 21.2 0.3 9-Jul-15 23.6 11.3 17.5 0.2

10-Jul-15 25.5 11.0 18.3 0.9 11-Jul-15 26.7 8.7 17.7 0.6 12-Jul-15 26.3 12.5 19.4 0.2 13-Jul-15 27.5 11.5 19.5 0.3 14-Jul-15 29.3 11.2 20.3 1.0 15-Jul-15 27.7 15.5 21.6 0.0 16-Jul-15 23.2 8.3 15.8 M

17-Jul-15 23.9 6.0 15.0 0.2 18-Jul-15 23.4 7.4 15.4 0.9 19-Jul-15 17.5 14.4 16.0 15.1 20-Jul-15 19.8 15.0 17.4 0.5 21-Jul-15 22.5 14.5 18.5 6.3 22-Jul-15 20.3 14.4 17.4 3.5 23-Jul-15 22.8 14.1 18.5 10.0 24-Jul-15 19.5 13.5 16.5 0.2 25-Jul-15 18.8 13.2 16.0 1.5 26-Jul-15 21.1 12.9 17.0 0.0 27-Jul-15 20.5 14.6 17.6 1.6 28-Jul-15 22.5 16.1 19.3 2.7 29-Jul-15 24.2 12.2 18.2 1.8 30-Jul-15 25.9 10.6 18.3 0.2 31-Jul-15 27.1 13.3 20.2 16.5 Sum 66.0* Avg 24.1 12.0 18.0

* M = missing data





August oC oC oC mm 1-Aug-15 27.8 11.7 19.8 0.6 2-Aug-15 25.0 11.4 18.2 0.6 3-Aug-15 29.4 10.4 19.9 0.9 4-Aug-15 29.5 18.4 24.0 11.5 5-Aug-15 26.6 14.0 20.3 1.9 6-Aug-15 22.7 12.1 17.4 0.0 7-Aug-15 24.4 9.7 17.1 0.3 8-Aug-15 25.2 13.5 19.4 0.7 9-Aug-15 20.2 14.9 17.6 21.6

10-Aug-15 23.4 9.8 16.6 0.2 11-Aug-15 25.5 8.3 16.9 0.2 12-Aug-15 23.9 17.1 20.5 14.4 13-Aug-15 26.1 18.1 22.1 0.0 14-Aug-15 25.6 12.3 19.0 0.0 15-Aug-15 25.5 11.9 18.7 1.3 16-Aug-15 25.4 15.5 20.5 0.5 17-Aug-15 28.0 14.7 21.4 0.2 18-Aug-15 29.6 14.6 22.1 1.2 19-Aug-15 30.6 16.9 23.8 0.8 20-Aug-15 29.9 17.4 23.7 1.0 21-Aug-15 28.8 21.3 25.1 0.0 22-Aug-15 29.0 22.1 25.6 3.2 23-Aug-15 27.1 21.1 24.1 0.5 24-Aug-15 28.7 18.4 23.6 0.0 25-Aug-15 28.6 15.4 22.0 0.0 26-Aug-15 21.6 16.5 19.1 0.0 27-Aug-15 23.0 12.9 18.0 19.2 28-Aug-15 22.2 12.0 17.1 0.0 29-Aug-15 23.1 9.1 16.1 1.0 30-Aug-15 24.1 12.2 18.2 0.3 31-Aug-15 26.4 11.1 18.8 2.9

Sum 85.0 Avg 26.0 14.4 20.2

* M = missing data





September oC oC oC mm 1-Sep-15 18.4 6.5 12.5 0.0 2-Sep-15 23.2 6.2 14.7 0.4 3-Sep-15 26.0 7.3 16.7 1.8 4-Sep-15 19.8 5.1 12.5 0.5 5-Sep-15 22.1 3.6 12.9 1.1 6-Sep-15 25.6 9.4 17.5 0.8 7-Sep-15 27.8 9.4 18.6 1.1 8-Sep-15 22.0 15.3 18.7 0.0 9-Sep-15 21.7 14.5 18.1 0.0

10-Sep-15 21.1 13.9 17.5 20.1 11-Sep-15 24.4 13.8 19.1 28.3 12-Sep-15 21.3 13.2 17.3 3.2 13-Sep-15 17.8 14.4 16.1 0.5 14-Sep-15 25.8 15.0 20.4 16.6 15-Sep-15 20.6 11.1 15.9 0.0 16-Sep-15 22.6 8.9 15.8 0.0 17-Sep-15 24.7 8.9 16.8 0.0 18-Sep-15 26.3 11.5 18.9 0.0 19-Sep-15 26.9 11.6 19.3 0.0 20-Sep-15 26.8 11.4 19.1 0.5 21-Sep-15 18.8 3.9 11.4 0.5 22-Sep-15 20.7 2.4 11.6 0.0 23-Sep-15 21.6 5.8 13.7 0.0 24-Sep-15 20.8 3.4 12.1 0.0 25-Sep-15 17.2 3.4 10.3 0.0 26-Sep-15 14.8 -1.0 6.9 0.0 27-Sep-15 17.6 -1.3 8.2 0.0 28-Sep-15 21.6 10.9 16.3 0.0 29-Sep-15 25.5 14.0 19.8 4.1 30-Sep-15 25.7 18.5 22.1 6.2

Sum 85.7 Avg 22.3 9.0 15.7





October oC oC oC mm

1-Oct-15 21.4 9.6 15.5 30.3

2-Oct-15 11.2 9.5 10.4 1.8

3-Oct-15 10.9 2.5 6.7 0.7

4-Oct-15 15.8 0.8 8.3 0.5

5-Oct-15 19.1 1.2 10.2 0.0

6-Oct-15 18.1 5.5 11.8 0.0

7-Oct-15 16.4 2.7 9.6 0.0

8-Oct-15 14.3 1.1 7.7 0.0

9-Oct-15 12.8 -0.6 6.1 16.2

10-Oct-15 13.4 -2.1 5.7 6.0

11-Oct-15 12.2 -1.5 5.4 0.2

12-Oct-15 19.1 8.9 14.0 0.3

13-Oct-15 19.4 10.8 15.1 15.8

14-Oct-15 20.4 8.2 14.3 0.0

15-Oct-15 12.9 -0.8 6.1 0.0

16-Oct-15 16.6 -1.7 7.5 4.0

17-Oct-15 11.7 -1.2 5.3 0.3

18-Oct-15 7.7 -3.8 2.0 0.0

19-Oct-15 5.8 -4.6 0.6 0.2

20-Oct-15 13.6 -1.8 5.9 0.2

21-Oct-15 10.2 -3.9 3.2 0.0

22-Oct-15 13.5 -4.4 4.6 5.5

23-Oct-15 13.1 5.0 9.1 0.3

24-Oct-15 9.5 -2.3 3.6 0.2

25-Oct-15 14.6 1.5 8.1 3.5

26-Oct-15 9.5 -2.5 3.5 0.0

27-Oct-15 M 5.3 E M M

28-Oct-15 9.6 -5.6 2.0 0.2

29-Oct-15 18.5 4.6 11.6 21.0

30-Oct-15 13.1 1.6 7.4 0.0

31-Oct-15 7.5 -0.1 3.7 0.0Sum 107.2*Avg 13.7 * 1 7.5*

* M = missing data E = estimated





November oC oC oC mm

1-Nov-15 11.2 1.0 6.1 6.9

2-Nov-15 12.2 2.6 7.4 0.2

3-Nov-15 6.2 -2.0 2.1 0.9

4-Nov-15 6.9 -3.1 1.9 1.0

5-Nov-15 6.5 -3.1 1.7 0.2

6-Nov-15 15.9 3.6 9.8 0.0

7-Nov-15 15.7 3.7 9.7 0.0

8-Nov-15 6.2 -2.0 2.1 0.0

9-Nov-15 10.5 -5.3 2.6 0.0

10-Nov-15 12.4 -1.8 5.3 0.9

11-Nov-15 7.3 -1.8 2.8 0.0

12-Nov-15 10.5 3.6 7.1 0.6

13-Nov-15 13.5 4.6 9.1 16.1

14-Nov-15 9.9 1.9 5.9 2.3

15-Nov-15 5.7 1.2 3.5 0.2

16-Nov-15 6.0 -2.3 1.9 2.4

17-Nov-15 3.5 0.1 1.8 0.2

18-Nov-15 1.9 -0.4 0.8 0.0

19-Nov-15 5.1 -1.0 2.1 0.3

20-Nov-15 8.7 5.1 6.9 0.0

21-Nov-15 9.9 6.8 8.4 6.1

22-Nov-15 15.0 9.8 12.4 41.0

23-Nov-15 17.2 0.2 8.7 43.8

24-Nov-15 0.2 -6.7 -3.3 0.0

25-Nov-15 0.3 -10.2 -5.0 1.0

26-Nov-15 7.0 -11.2 -2.1 0.5

27-Nov-15 14.4 4.8 9.6 0.0

28-Nov-15 10.1 -1.5 4.3 3.0

29-Nov-15 M 4.3 E M M

30-Nov-15 -2.8 -7.5 -5.2 0.0Sum 127.6*Avg 8.5* -0.5 4.1*






December oC oC oC mm

1-Dec-15 1.7 -7.4 -2.9 0.2

2-Dec-15 8.0 -4.6 1.7 5.8

3-Dec-15 8.1 0.5 4.3 17.8

4-Dec-15 0.6 -3.1 -1.3 2.6

5-Dec-15 1.3 -5.3 -2.0 0.4

6-Dec-15 8.2 -1.7 3.3 0.7

7-Dec-15 8.9 -2.1 3.4 0.0

8-Dec-15 3.6 -6.3 -1.4 0.2

9-Dec-15 6.2 0.0 3.1 0.0

10-Dec-15 4.9 -1.0 2.0 2.3

11-Dec-15 11.9 0.9 6.4 6.8

12-Dec-15 10.6 -0.2 5.2 0.0

13-Dec-15 5.9 -3.1 1.4 0.0

14-Dec-15 2.2 -2.4 -0.1 1.1

15-Dec-15 2.0 -0.2 0.9 26.2

16-Dec-15 2.1 -3.0 -0.5 0.5

17-Dec-15 4.7 -3.3 0.7 3.2

18-Dec-15 10.9 4.0 7.5 14.1

19-Dec-15 4.0 -2.4 0.8 0.3

20-Dec-15 -1.8 -8.6 -5.2 0.2

21-Dec-15 6.1 -9.5 -1.7 0.5

22-Dec-15 2.2 0.0 1.1 19.5

23-Dec-15 4.5 0.6 2.6 0.7

24-Dec-15 11.8 3.1 7.5 10.7

25-Dec-15 10.4 -0.9 4.8 1.0

26-Dec-15 5.9 -2.4 1.8 1.0

27-Dec-15 1.1 -2.0 -0.5 15.3

28-Dec-15 -1.7 -14.1 -7.9 0.4

29-Dec-15 -5.7 -15.1 -10.4 8.8

30-Dec-15 -2.7 -6.7 -4.7 0.7

31-Dec-15 2.8 -3.5 -0.4 2.7Sum 143.7Avg 4.5 -3.2 0.6





January oC oC oC mm

1-Jan-16 1.0 -13.1 -6.1 0.0

2-Jan-16 1.0 -14.9 -7.0 0.5

3-Jan-16 1.6 -1.7 -0.1 1.0

4-Jan-16 -0.1 -11.5 -5.8 0.2

5-Jan-16 -7.9 -19.9 -13.9 0.0

6-Jan-16 -0.5 -17.9 -9.2 0.0

7-Jan-16 1.2 -17.7 -8.3 0.7

8-Jan-16 -2.0 -15.8 -8.9 0.9

9-Jan-16 -2.0 -5.1 -3.6 0.0

10-Jan-16 6.6 -2.0 2.3 0.0

11-Jan-16 10.0 -4.1 3.0 29.0

12-Jan-16 -2.6 -8.5 -5.6 1.0

13-Jan-16 0.7 -7.2 -3.3 12.1

14-Jan-16 -6.6 -17.9 -12.3 0.4

15-Jan-16 -6.1 -14.3 -10.2 0.2

16-Jan-16 -2.4 -13.1 -7.8 9.4

17-Jan-16 0.0 -6.1 -3.1 0.3

18-Jan-16 -2.3 -9.5 -5.9 0.8

19-Jan-16 M 10.5 E M M

20-Jan-16 -1.1 -9.6 -5.4 0.0

21-Jan-16 -6.3 -12.3 -9.3 0.0

22-Jan-16 -6.5 -18.9 -12.7 0.0

23-Jan-16 -5.3 -19.1 -12.2 0.2

24-Jan-16 -2.6 -13.8 -8.2 0.0

25-Jan-16 -1.9 -18.8 -10.4 0.0

26-Jan-16 6.0 -18.4 -6.2 0.0

27-Jan-16 5.1 -4.3 0.4 0.0

28-Jan-16 2.3 -11.8 -4.8 0.0

29-Jan-16 2.8 -1.9 0.5 7.7

30-Jan-16 -1.2 -4.1 -2.7 5.2

31-Jan-16 3.2 -2.9 0.2 2.5Sum 72.1*Avg 0.5* -11.2 5.9*






February oC oC oC mm

1-Feb-16 9.7 0.3 5.0 0.0

2-Feb-16 1.8 -14.2 -6.2 0.0

3-Feb-16 5.2 -16.3 -5.6 1.7

4-Feb-16 9.0 1.7 5.4 7.2

5-Feb-16 3.6 -4.5 -0.5 30.5

6-Feb-16 -3.0 -9.5 -6.3 0.0

7-Feb-16 3.1 -10.4 -3.7 0.7

8-Feb-16 -3.4 -9.6 -6.5 3.3

9-Feb-16 -2.5 -4.5 -3.5 1.9

10-Feb-16 -1.6 -11.9 -6.8 0.2

11-Feb-16 -0.5 -11.3 -5.9 1.8

12-Feb-16 -5.5 -14.5 -10.0 0.2

13-Feb-16 -8.5 -14.8 -11.7 0.5

14-Feb-16 -8.8 -19.3 -14.1 0.0

15-Feb-16 -7.5 -21.4 -14.5 0.0

16-Feb-16 12.1 -12.3 -0.1 2.1

17-Feb-16 12.1 -1.6 5.3 16.6

18-Feb-16 2.3 -6.2 -2.0 0.3

19-Feb-16 0.9 -9.7 -4.4 0.0

20-Feb-16 5.5 -10.6 -2.6 1.5

21-Feb-16 5.8 0.9 3.4 4.5

22-Feb-16 1.2 -11.9 -5.4 0.5

23-Feb-16 -2.4 -14.1 -8.3 0.4

24-Feb-16 6.8 -11.5 -2.4 1.8

25-Feb-16 14.4 5.9 10.2 8.2

26-Feb-16 6.0 -5.0 0.5 2.2

27-Feb-16 -1.9 -8.8 -5.4 0.0

28-Feb-16 6.3 -3.5 1.4 0.0

29-Feb-16 15.9 -2.9 6.5 0.5Sum 86.6Avg 2.6 -8.7 -3.0





March oC oC oC mm

1-Mar-16 5.4 -7.9 -1.3 0.5

2-Mar-16 11.3 -8.2 1.6 24.0

3-Mar-16 -0.6 -12.8 -6.7 0.0

4-Mar-16 -3.2 -15.4 -9.3 0.2

5-Mar-16 -3.6 -8.9 -6.3 1.5

6-Mar-16 -0.8 -14.8 -7.8 0.2

7-Mar-16 5.2 -13.0 -3.9 0.0

8-Mar-16 5.4 -8.3 -1.5 0.0

9-Mar-16 3.7 -8.4 -2.4 5.6

10-Mar-16 7.2 -2.5 2.4 0.5

11-Mar-16 -0.9 -10.9 -5.9 6.2

12-Mar-16 1.1 -9.1 -4.0 0.2

13-Mar-16 4.9 -3.8 0.6 0.9

14-Mar-16 1.2 -7.1 -3.0 0.0

15-Mar-16 4.3 -9.3 -2.5 3.8

16-Mar-16 10.7 0.3 5.5 4.7

17-Mar-16 2.7 -1.7 0.5 1.6

18-Mar-16 2.4 -5.5 -1.6 3.9

19-Mar-16 -1.6 -9.9 -5.8 0.2

20-Mar-16 -0.8 -11.7 -6.3 0.0

21-Mar-16 3.1 -7.8 -2.4 14.2

22-Mar-16 -1.7 -9.1 -5.4 0.0

23-Mar-16 3.3 -10.3 -3.5 3.9

24-Mar-16 -2.8 -11.1 -7.0 0.0

25-Mar-16 4.5 -4.5 0.0 10.7

26-Mar-16 0.0 -8.0 -4.0 0.0

27-Mar-16 6.3 -8.6 -1.2 0.3

28-Mar-16 8.2 -1.2 3.5 6.8

29-Mar-16 8.3 -2.6 2.9 4.4

30-Mar-16 4.8 -4.8 0.0 0.0

31-Mar-16 12.6 0.7 6.7 0.0Sum 94.3Avg 3.3 -7.6 -2.2





April oC oC oC mm

1-Apr-16 17.5 8.8 13.2 6.3

2-Apr-16 9.4 5.2 7.3 10.1

3-Apr-16 6.9 -7.5 -0.3 2.9

4-Apr-16 -1.8 -9.3 -5.6 0.2

5-Apr-16 -2.2 -12.2 -7.2 0.0

6-Apr-16 1.3 -11.3 -5.0 0.0

7-Apr-16 13.2 -1.3 6.0 0.0

8-Apr-16 12.8 7.6 10.2 11.4

9-Apr-16 9.4 1.1 5.3 0.2

10-Apr-16 2.2 -7.2 -2.5 3.2

11-Apr-16 8.7 -9.0 -0.2 2.3

12-Apr-16 14.1 6.4 10.3 3.1

13-Apr-16 11.4 -1.8 4.8 12.3

14-Apr-16 7.5 -3.5 2.0 0.0

15-Apr-16 5.5 0.2 2.9 0.4

16-Apr-16 3.6 -1.4 1.1 0.0

17-Apr-16 13.6 -1.8 5.9 1.0

18-Apr-16 8.0 -3.5 2.3 0.0

19-Apr-16 8.5 -3.1 2.7 0.2

20-Apr-16 9.1 -4.4 2.4 0.0

21-Apr-16 18.3 -5.2 6.6 0.3

22-Apr-16 20.2 -0.1 10.1 0.8

23-Apr-16 18.9 3.7 11.3 8.1

24-Apr-16 8.0 -5.3 1.4 0.4

25-Apr-16 8.9 -6.7 1.1 0.5

26-Apr-16 8.5 -6.8 0.9 1.1

27-Apr-16 M 6.1E M M

28-Apr-16 4.7 -7.6 -1.5 0.0

29-Apr-16 9.0 -8.8 0.1 0.2

30-Apr-16 14.6 -7.2 3.7 0.0Sum 65.0*Avg 9.3* -3.3 3.1*






May oC oC oC mm

1-May-16 17.3 -4.3 6.5 0.6

2-May-16 15.7 2.6 9.2 6.5

3-May-16 11.8 3.0 7.4 0.7

4-May-16 8.7 2.8 5.8 0.0

5-May-16 7.6 3.8 5.7 27.5

6-May-16 9.3 5.0 7.2 10.8

7-May-16 17.5 5.2 11.4 0.5

8-May-16 20.1 7.4 13.8 0.4

9-May-16 9.3 3.6 6.5 0.0

10-May-16 11.6 5.3 8.5 0.0

11-May-16 12.9 -1.2 5.9 0.0

12-May-16 14.9 -2.8 6.1 0.0

13-May-16 19.1 -2.6 8.3 0.0

14-May-16 15.0 5.3 10.2 4.8

15-May-16 19.7 5.9 12.8 1.1

16-May-16 9.3 4.1 6.7 1.7

17-May-16 13.9 2.4 8.2 0.0

18-May-16 17.3 0.2 8.8 0.0

19-May-16 21.4 0.4 10.9 0.0

20-May-16 M 8.0 E M M

21-May-16 23.7 2.2 13.0 0.2

22-May-16 20.0 8.0 14.0 2.0

23-May-16 15.7 8.8 12.3 0.0

24-May-16 22.8 7.9 15.4 0.5

25-May-16 23.7 10.3 17.0 0.0

26-May-16 19.9 6.4 13.2 0.0

27-May-16 15.0 6.1 10.6 3.6

28-May-16 15.9 5.2 10.6 2.3

29-May-16 19.8 5.7 12.8 0.0

30-May-16 16.2 8.1 12.2 7.6

31-May-16 24.7 11.2 18.0 0.0Sum 70.8*Avg 16.3* 4.3 10.3*






June oC oC oC mm

1-Jun-16 15.6 0.5 8.1 0.0

2-Jun-16 19.1 0.1 9.6 0.2

3-Jun-16 19.0 5.7 12.4 0.0

4-Jun-16 22.0 8.5 15.3 0.0

5-Jun-16 19.8 11.1 15.5 0.0

6-Jun-16 14.6 10.2 12.4 1.2

7-Jun-16 24.2 11.6 17.9 0.2

8-Jun-16 19.4 11.6 15.5 35.1

9-Jun-16 12.4 7.3 9.9 2.1

10-Jun-16 14.5 5.6 10.1 0.6

11-Jun-16 21.0 5.5 13.3 0.6

12-Jun-16 14.8 10.4 12.6 2.8

13-Jun-16 15.6 9.8 12.7 3.5

14-Jun-16 11.9 8.2 10.1 1.9

15-Jun-16 12.7 8.3 10.5 1.4

16-Jun-16 13.9 5.3 9.6 0.2

17-Jun-16 18.5 3.8 11.2 0.2

18-Jun-16 21.0 2.2 11.6 0.0

19-Jun-16 24.3 10.7 17.5 0.0

20-Jun-16 26.6 9.0 17.8 0.0

21-Jun-16 26.4 10.1 18.3 0.0

22-Jun-16 24.7 13.0 18.9 3.3

23-Jun-16 23.1 9.6 16.4 0.2

24-Jun-16 22.5 6.5 14.5 0.0

25-Jun-16 26.6 5.1 15.9 1.1

26-Jun-16 29.9 8.3 19.1 1.6

27-Jun-16 27.4 11.1 19.3 0.2

28-Jun-16 28.4 10.7 19.6 0.3

29-Jun-16 25.5 17.7 21.6 0.0

30-Jun-16 26.3 14.8 20.6 4.4Sum 61.1Avg 20.7 8.4 14.6





July oC oC oC mm

1-Jul-16 28.9 12.2 20.6 0.0

2-Jul-16 23.2 12.7 18.0 4.4

3-Jul-16 22.0 9.3 15.7 0.0

4-Jul-16 24.9 6.7 15.8 0.9

5-Jul-16 29.3 14.2 21.8 0.4

6-Jul-16 22.3 12.8 17.6 1.7

7-Jul-16 20.2 10.7 15.5 24.0

8-Jul-16 17.2 10.7 14.0 0.6

9-Jul-16 18.7 11.6 15.2 0.0

10-Jul-16 15.9 13.0 14.5 9.0

11-Jul-16 20.2 8.8 14.5 1.5

12-Jul-16 25.8 7.0 16.4 10.8

13-Jul-16 28.6 9.2 18.9 0.2

14-Jul-16 28.8 14.4 21.6 0.5

15-Jul-16 26.7 16.3 21.5 9.4

16-Jul-16 30.9 16.8 23.9 0.6

17-Jul-16 25.1 14.4 19.8 0.4

18-Jul-16 28.1 17.1 22.6 0.0

19-Jul-16 22.8 9.9 16.4 0.0

20-Jul-16 22.1 8.4 15.3 0.0

21-Jul-16 25.6 9.3 17.5 0.3

22-Jul-16 29.3 11.3 20.3 1.0

23-Jul-16 26.7 15.1 20.9 2.7

24-Jul-16 29.5 13.6 21.6 3.5

25-Jul-16 29.9 15.3 22.6 0.2

26-Jul-16 24.0 17.3 20.7 2.7

27-Jul-16 28.1 16.4 22.3 0.0

28-Jul-16 29.8 15.2 22.5 0.8

29-Jul-16 26.4 17.7 22.1 6.8

30-Jul-16 26.8 12.1 19.5 0.2

31-Jul-16 25.9 10.9 18.4 0.7Sum 83.3Avg 25.3 12.6 19.0





August oC oC oC mm

1-Aug-16 26.9 10.9 18.9 0.6

2-Aug-16 25.3 10.5 17.9 0.9

3-Aug-16 26.6 9.1 17.9 0.0

4-Aug-16 23.7 11.7 17.7 13.0

5-Aug-16 26.3 13.6 20.0 0.0

6-Aug-16 28.6 13.2 20.9 0.0

7-Aug-16 24.9 13.0 19.0 0.0

8-Aug-16 23.1 10.3 16.7 0.8

9-Aug-16 22.4 9.3 15.9 0.0

10-Aug-16 27.9 7.2 17.6 0.0

11-Aug-16 26.1 14.7 20.4 0.8

12-Aug-16 22.2 12.5 17.4 1.0

13-Aug-16 25.2 10.1 17.7 0.2

14-Aug-16 16.9 14.3 15.6 28.3

15-Aug-16 21.1 11.8 16.5 0.2

16-Aug-16 24.5 11.1 17.8 0.3

17-Aug-16 19.9 14.9 17.4 53.8

18-Aug-16 24.4 14.9 19.7 0.0

19-Aug-16 25.0 13.0 19.0 0.8

20-Aug-16 25.3 13.2 19.3 0.3

21-Aug-16 26.3 15.8 21.1 0.0

22-Aug-16 28.1 16.5 22.3 11.5

23-Aug-16 23.4 12.5 18.0 0.0

24-Aug-16 25.1 11.6 18.4 0.2

25-Aug-16 27.9 11.3 19.6 0.0

26-Aug-16 24.6 18.3 21.5 0.0

27-Aug-16 23.2 9.7 16.5 0.2

28-Aug-16 23.5 8.1 15.8 1.5

29-Aug-16 22.2 14.3 18.3 1.2

30-Aug-16 22.7 10.9 16.8 0.0

31-Aug-16 23.5 12.1 17.8 0.4Sum 116.0Avg 24.4 12.3 18.4



Appendix 2.

ANOVA tables for the data presented in the following pages were produced from Microsoft Excel 2007 data analysis tool.

The Tukey procedure was taken from: Steele, R.G.D and J.H. Torrie, 1980. Principles and Procedures of Statistics; A Biometrical Approach. 2nd ed. 633 pp.

EFFICACY/CROP TOLERANCE PERFORMANCE FORM Page 34 of 69 Tolerance Study AAFC15-068T-200 Indaziflam / Lowbush Blueberry Appendix 2-1: Stem height (cm); 4 plots per treatment, with 25 stems per plot. June 24 2015 - Page 1 of 2

UTC Alion 375 mL/ha

9 8 6 6 4 9 10 33

9 3 9 4 9 6 9 4

7 5 5 3 9 6 5 5

7 5 7 7 7 7 4 5

8 7 8 9 4 10 9 4

10 4 7 8 5 6 2 13

5 5 5 5 4 3 3 8

4 5 9 5 12 7 3 5

6 9 8 5 6 5 10 5

5 10 9 8 6 8 4 9

5 7 5 10 6 2 5 6

6 6 10 6 6 9 7 8

2 6 10 5 4 3 4 8

4 3 4 10 6 4 6 15

5 4 5 8 5 4 4 10

6 7 7 12 6 7 6 8

6 9 6 7 8 2 5 7

8 7 11 6 9 8 7 10

8 10 7 12 7 3 10 5

5 9 5 8 7 7 9 6

6 9 6 6 9 4 18 7

5 7 3 7 10 8 5 3

6 4 7 3 9 5 4 7

4 5 6 5 8 7 6 7

6 6 8 6 5 6 8 6

Plot Means 6.08 6.4 6.92 6.84 6.84 5.84 6.52 8.16

Std. Error 0.37 0.42 0.40 0.49 0.43 0.45 0.68 1.17

Aver. 6.56 Aver. 6.84

S.E. 0.21 S.E. 0.38

EFFICACY/CROP TOLERANCE PERFORMANCE FORM Page 35 of 69 Tolerance Study AAFC15-068T-200 Indaziflam / Lowbush Blueberry Appendix 2-2: Stem height (cm); 4 plots per treatment, with 25 stems per plot. June 24 2015 - Page 2 of 2

Alion 750 mL/ha Velpar DF 2.22 kg/ha

10 6 6 5 4 7 8 4

5 6 2 1 5 8 5 6

5 5 7 3 6 5 5 5

4 7 5 4 5 6 5 6

4 5 8 5 9 5 5 4

6 4 3 5 6 6 5 5

6 7 3 9 6 6 5 6

5 7 8 5 8 6 4 7

5 5 13 6 7 4 4 8

7 6 4 6 8 4 3 8

6 9 7 3 8 8 6 3

2 8 8 3 7 8 6 6

7 7 5 5 9 8 4 4

4 7 4 5 4 7 8 6

5 9 9 8 5 5 8 6

4 8 8 9 8 8 5 9

6 8 6 3 8 8 4 8

4 2 4 5 8 8 5 5

3 6 3 8 7 8 2 5

2 7 6 8 6 12 4 4

2 4 5 4 9 10 7 5

3 2 5 7 4 5 6 9

3 11 3 5 2 6 5 3

3 6 6 5 11 5 3 5

4 6 5 7 3 7 16 5

Plot Means 4.6 6.32 5.72 5.36 6.52 6.8 5.52 5.68

Std. Error 0.37 0.41 0.49 0.41 0.43 0.37 0.53 0.34

Aver. 5.5 Aver. 6.13

S.E. 0.22 S.E. 0.22

EFFICACY/CROP TOLERANCE PERFORMANCE FORM Page 36 of 69 Tolerance Study AAFC15-068T-200 Indaziflam / Lowbush Blueberry Appendix 2-3: Stem height (cm) ANOVA; 100 data points. June 24 2015 Anova: Single Factor Stem Height (cm) June 24 2015

25 values from each of 4 plots.

SUMMARY

Groups Count Sum Average Variance Tukey

UTC 100 684 6.84 14.20 b

Alion 375 mL/ha 100 656 6.56 4.45 b

Alion 750 mL/ha 100 550 5.50 4.70 a

Velpar DF 2.22 kg/ha 100 613 6.13 4.70 ab

ANOVA

Source of Variation SS df MS F P‐value F crit

Between Groups 102.09 3 34.03 4.85 0.0025 2.63

Within Groups 2776.39 396 7.01

Total 2878.48 399

Tukey's HSD (0.05 level)

MS 7.01

r 100 replicates

MS/r 0.07

Sy 0.2648

p 4 treatments

error df 396

q .05 3.86

1.02

EFFICACY/CROP TOLERANCE PERFORMANCE FORM Page 37 of 69 Tolerance Study AAFC15-068T-200 Indaziflam / Lowbush Blueberry Appendix 2-4: Stem height (cm); 4 plots per treatment, with 25 stems per plot. July 7 2015 - Page 1 of 2

UTC Alion 375 mL/ha

14 11 15 14 10 18 11 9

17 11 13 11 13 13 14 15

14 5 14 11 9 14 13 11

5 7 19 12 14 18 11 15

14 8 13 10 11 11 10 22

15 23 13 16 11 11 14 10

13 18 14 13 12 13 6 9

13 11 5 9 12 9 8 14

16 19 12 18 10 13 11 12

11 8 16 13 10 13 14 12

7 12 9 16 14 11 17 14

6 18 11 8 12 10 11 19

7 14 10 14 17 10 14 21

11 10 11 13 12 13 7 6

14 11 12 15 12 4 10 5

6 12 9 11 11 10 11 17

14 10 10 12 11 13 12 14

7 9 16 8 12 11 15 9

6 15 10 16 14 12 5 13

10 16 12 12 13 7 18 5

10 7 14 17 14 15 8 5

12 19 11 11 16 7 11 10

12 13 11 15 12 8 11 12

8 11 18 6 14 9 16 13

14 17 18 9 7 14 13 12

Plot Means 11.04 12.6 12.64 12.4 12.12 11.48 11.64 12.16

Std. Error 0.72 0.90 0.65 0.62 0.43 0.65 0.66 0.92

Aver. 12.17 Aver. 11.85

S.E. 0.37 S.E. 0.34



7 13 8 9 12 11 4 9

16 14 11 7 18 15 12 12

11 10 17 13 16 12 11 15

14 10 8 7 11 12 15 12

9 14 15 13 14 14 13 9

15 13 10 15 10 16 8 11

12 12 13 8 13 15 9 11

13 11 10 11 12 14 10 10

11 14 16 13 14 16 11 15

7 12 7 12 17 14 10 13

13 12 10 5 14 18 10 14

12 13 12 4 20 13 12 10

14 15 12 8 6 14 12 9

15 10 11 12 7 11 7 8

9 11 13 14 12 13 9 8

12 14 8 9 12 17 7 10

8 17 12 14 13 7 6 13

9 9 12 14 13 12 9 7

8 11 6 7 16 8 12 3

8 13 9 8 9 9 11 12

8 12 15 10 14 13 10 14

12 8 6 8 14 12 14 11

9 15 15 13 13 6 22 14

8 7 13 12 9 16 21 18

8 10 12 10 11 14 22 15

Plot Means 10.72 12 11.24 10.24 12.8 12.88 11.48 11.32

Std. Error 0.56 0.47 0.61 0.61 0.65 0.60 0.91 0.64

Aver. 11.05 Aver. 12.12

S.E. 0.29 S.E. 0.36

EFFICACY/CROP TOLERANCE PERFORMANCE FORM Page 39 of 69 Tolerance Study AAFC15-068T-200 Indaziflam / Lowbush Blueberry Appendix 2-6: Stem height (cm) ANOVA; 100 data points. July 7 2015 Anova: Single Factor Stem Height (cm) July 7 2015


SUMMARY

Groups Count Sum Average Variance

UTC 100 1217 12.17 13.35

Alion 375 mL/ha 100 1185 11.85 11.48

Alion 750 mL/ha 100 1105 11.05 8.21

Velpar DF 2.22 kg/ha 100 1212 12.12 12.79

ANOVA


Between Groups 80.43 3 26.81 2.34 0.073 2.63

Within Groups 4538.17 396 11.46

Total 4618.60 399

Calculated F is less than the critical F.

There are no significant differences at the 0.05 level.


UTC Alion 375 mL/ha

17 18 23 18 18 22 22 16

18 17 22 18 18 17 19 22

18 16 22 20 15 16 17 23

15 16 29 22 15 23 18 9

17 18 20 17 15 21 24 20

16 18 26 25 16 18 18 24

16 24 16 20 18 14 13 19

16 27 16 16 20 16 14 10

18 27 18 26 15 20 17 12

10 26 20 23 16 19 17 17

21 17 14 16 24 20 26 8

11 22 15 16 15 17 17 21

15 21 18 21 21 8 23 20

12 24 11 20 19 17 10 20

18 20 8 15 12 18 15 26

15 18 15 23 12 17 18 12

22 15 5 20 16 14 15 15

23 19 21 28 15 26 23 21

14 19 13 20 17 16 26 20

17 23 16 24 18 15 27 22

15 19 22 20 24 14 19 18

19 18 18 25 20 17 16 14

21 22 16 24 21 9 22 22

22 16 18 18 16 16 16 12

17 19 28 10 15 17 17 12

Plot Means 16.92 19.96 18 20.2 17.24 17.08 18.76 17.4

Std. Error 0.66 0.71 1.13 0.81 0.63 0.78 0.87 1.01

Aver. 18.77 Aver. 17.62

S.E. 0.44 S.E. 0.42



11 22 23 14 21 16 18 16

21 20 14 10 20 23 16 20

22 18 17 10 27 25 27 16

14 16 23 12 18 22 18 21

15 16 16 22 10 18 12 12

13 20 18 19 21 20 18 16

17 19 22 18 18 25 17 17

16 23 9 18 19 19 18 16

19 19 20 14 20 24 15 18

18 15 23 14 24 26 17 19

16 17 12 19 26 27 23 19

21 17 18 21 27 23 24 20

21 18 15 12 20 18 20 15

21 18 18 18 15 17 15 16

20 22 18 18 17 21 20 12

22 20 18 21 17 22 16 14

17 22 19 20 22 25 6 12

10 15 21 14 18 16 13 15

12 16 19 18 17 13 23 15

12 22 15 20 19 16 14 16

9 20 17 15 19 9 18 17

10 12 19 31 20 10 18 14

13 19 20 16 17 13 15 21

18 13 18 23 15 18 22 17

17 14 14 21 17 17 19 19

Plot Means 16.2 18.12 17.84 17.52 19.36 19.32 17.68 16.52

Std. Error 0.82 0.60 0.69 0.93 0.77 0.99 0.86 0.53

Aver. 17.42 Aver. 18.22

S.E. 0.39 S.E. 0.41



SUMMARY


UTC 100 1877 18.77 19.35

Alion 375 mL/ha 100 1762 17.62 17.29

Alion 750 mL/ha 100 1742 17.42 14.95

Velpar DF 2.22 kg/ha 100 1822 18.22 17.20

ANOVA


Between Groups 112.19 3 37.40 2.17 0.0905 2.63

Within Groups 6810.79 396 17.20

Total 6922.98 399



EFFICACY/CROP TOLERANCE PERFORMANCE FORM Page 43 of 69 Tolerance Study AAFC15-068T-200 Indaziflam / Lowbush Blueberry Appendix 2-10: Stem frequency; 4 plots per treatment, with 25 stems per plot. June 24 2015 - Page 1 of 2

UTC Alion 375 mL/ha

1 0 0 0 0 1 0 0

1 0 0 0 0 1 1 0

1 0 1 1 1 0 0 1

0 0 1 0 1 0 0 1

0 1 1 1 1 1 1 0

1 1 1 1 1 1 0 0

1 1 0 0 1 0 1 0

1 1 0 1 1 0 1 0

0 0 1 1 0 1 1 1

0 1 1 0 1 0 1 0

0 0 0 0 0 0 1 1

0 1 1 1 1 1 0 0

0 0 1 1 0 1 1 1

0 0 1 0 1 1 0 0

0 1 0 0 1 0 1 0

1 0 1 0 1 0 0 0

1 0 0 0 1 1 1 1

0 1 0 0 0 1 1 0

1 0 0 1 1 0 0 0

0 1 1 1 1 1 1 1

0 1 1 0 1 0 1 1

0 0 1 1 1 1 0 0

1 0 0 1 1 0 1 0

0 0 1 0 1 0 1 0

0 0 0 0 0 1 0 0

Plot Means 0.4 0.4 0.56 0.44 0.72 0.52 0.6 0.32

Std. Error 0.10 0.10 0.10 0.10 0.09 0.10 0.10 0.10

Aver. 0.45 Aver. 0.54

S.E. 0.05 S.E. 0.05

EFFICACY/CROP TOLERANCE PERFORMANCE FORM Page 44 of 69 Tolerance Study AAFC15-068T-200 Indaziflam / Lowbush Blueberry Appendix 2-11: Stem frequency; 4 plots per treatment, with 25 stems per plot. June 24 2015 - Page 2 of 2


1 1 0 1 0 0 1 0

1 1 0 0 0 0 0 1

0 1 1 0 0 0 0 1

0 1 0 0 0 1 1 1

0 1 0 1 1 1 1 1

0 1 0 0 1 0 1 1

1 1 0 0 1 1 1 0

1 1 0 0 0 1 0 1

0 0 0 1 0 1 1 1

1 0 1 0 1 1 0 1

1 0 1 1 1 1 1 0

0 0 1 0 1 1 1 1

1 0 0 0 0 0 0 0

0 1 0 0 1 1 1 1

1 1 1 0 1 1 1 0

1 1 1 1 1 1 0 1

0 1 0 1 1 1 0 1

1 0 1 0 1 0 1 0

1 0 0 0 1 0 1 1

0 0 1 0 0 1 0 0

0 1 0 0 1 0 1 1

0 0 0 0 1 1 1 0

1 1 1 1 1 0 0 1

0 0 0 0 1 1 1 1

0 1 1 0 1 1 0 1

Plot Means 0.48 0.6 0.4 0.28 0.68 0.64 0.6 0.68

Std. Error 0.10 0.10 0.10 0.09 0.10 0.10 0.10 0.10

Aver. 0.44 Aver. 0.65

S.E. 0.05 S.E. 0.05

EFFICACY/CROP TOLERANCE PERFORMANCE FORM Page 45 of 69 Tolerance Study AAFC15-068T-200 Indaziflam / Lowbush Blueberry Appendix 2-12: Stem frequency ANOVA; 100 data points. June 24 2015 Anova: Single Factor Stem Frequency June 24 2015


SUMMARY


UTC 100 45 0.45 0.25 a

Alion 375 mL/ha 100 54 0.54 0.25 ab

Alion 750 mL/ha 100 44 0.44 0.25 a

Velpar DF 2.22 kg/ha 100 65 0.65 0.23 b

ANOVA


Between Groups 2.86 3 0.95 3.89 0.0092 2.63


Total 99.84 399


MS 0.24

r 100 replicates

MS/r 0.00

Sy 0.0495

p 4 treatments

error df 396

q .05 3.86

0.19

EFFICACY/CROP TOLERANCE PERFORMANCE FORM Page 46 of 69 Tolerance Study AAFC15-068T-200 Indaziflam / Lowbush Blueberry Appendix 2-13: Stem frequency; 4 plots per treatment, with 25 stems per plot. July 7 2015 - Page 1 of 2

UTC Alion 375 mL/ha

1 0 1 0 1 1 1 1

1 0 1 0 1 1 1 0

1 1 1 0 1 1 0 0

0 1 1 0 1 1 1 0

1 1 1 1 1 1 1 0

1 1 1 1 1 0 0 0

1 0 1 1 1 1 1 0

1 0 1 1 1 1 1 1

0 1 1 1 1 1 1 1

1 0 1 1 1 0 1 0

1 0 0 1 1 0 1 0

0 1 1 1 1 1 1 1

1 1 1 1 1 1 1 0

1 1 0 1 1 1 0 1

1 1 0 1 1 1 1 1

0 1 1 1 1 1 0 1

1 1 0 1 1 0 1 1

1 1 1 0 1 1 1 1

1 1 0 1 1 1 1 1

1 1 1 1 1 1 1 1

1 0 1 1 1 0 1 1

1 1 1 1 1 0 0 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 0

0 0 1 1 1 0 0 1

Plot Means 0.80 0.68 0.80 0.80 1.00 0.72 0.76 0.60

Std. Error 0.08 0.10 0.08 0.08 0.00 0.09 0.09 0.10

Aver. 0.77 Aver. 0.77

S.E. 0.04 S.E. 0.04



1 1 0 0 1 1 0 0

1 1 0 0 1 1 1 1

1 1 1 1 1 1 1 1

1 1 0 1 1 1 1 1

1 1 0 0 0 1 1 1

0 1 0 1 1 1 1 1

1 1 1 1 1 1 0 1

1 1 0 1 1 1 1 1

1 0 0 1 1 1 0 1

0 1 1 1 1 1 1 1

1 0 1 1 1 1 0 1

0 1 1 0 1 1 1 1

1 0 1 0 0 0 1 1

1 1 1 1 1 1 1 1

0 1 1 1 1 1 1 1

0 1 1 1 1 1 0 1

1 0 1 1 1 1 1 1

1 1 1 1 1 1 1 0

1 0 0 1 1 1 1 1

0 1 1 0 1 1 0 1

0 1 1 1 1 0 0 1

1 1 1 1 1 1 0 1

1 1 1 1 1 0 0 1

0 1 0 0 1 1 1 1

1 1 1 1 1 1 1 1

Plot Means 0.68 0.80 0.64 0.72 0.92 0.88 0.64 0.92

Std. Error 0.10 0.08 0.10 0.09 0.06 0.07 0.10 0.06

Aver. 0.71 Aver. 0.84

S.E. 0.05 S.E. 0.04

EFFICACY/CROP TOLERANCE PERFORMANCE FORM Page 48 of 69 Tolerance Study AAFC15-068T-200 Indaziflam / Lowbush Blueberry Appendix 2-15: Stem frequency ANOVA; 100 data points. July 7 2015 Anova: Single Factor Stem Frequency July 7 2015


SUMMARY


UTC 100 77 0.77 0.18

Alion 375 mL/ha 100 77 0.77 0.18

Alion 750 mL/ha 100 71 0.71 0.21

Velpar DF 2.22 kg/ha 100 84 0.84 0.14

ANOVA


Between Groups 0.85 3 0.28 1.61 0.1863 2.63


Total 70.30 399




UTC Alion 375 mL/ha

1 1 1 0 1 1 1 1

1 0 1 1 1 1 1 1

1 0 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 0

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 0

1 1 1 1 1 1 1 0

1 0 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 0

1 1 1 1 1 1 1 1

1 1 1 0 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 0 1 1 1 1

1 1 1 0 1 1 1 1

1 1 1 0 1 1 1 1

1 0 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

0 1 1 1 1 1 1 1

Plot Means 0.96 0.84 1.00 0.80 1.00 1.00 1.00 0.84

Std. Error 0.04 0.07 0.00 0.08 0.00 0.00 0.00 0.07

Aver. 0.90 Aver. 0.96

S.E. 0.03 S.E. 0.02



1 1 1 1 1 1 1 1

1 1 1 0 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 0 1 1 1 1

1 0 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 0

1 1 1 1 1 1 1 1

1 1 1 1 1 1 0 1

1 1 1 1 1 1 1 0

0 1 1 1 1 1 1 1

1 1 0 1 1 1 1 1

1 1 1 1 1 1 0 1

1 1 1 1 1 1 0 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

0 1 1 1 1 1 1 1

Plot Means 0.92 0.96 0.96 0.92 1.00 1.00 0.88 0.92

Std. Error 0.06 0.04 0.04 0.06 0.00 0.00 0.07 0.06

Aver. 0.94 Aver. 0.95

S.E. 0.02 S.E. 0.02



SUMMARY


UTC 100 90 0.90 0.09

Alion 375 mL/ha 100 96 0.96 0.04

Alion 750 mL/ha 100 94 0.94 0.06

Velpar DF 2.22 kg/ha 100 95 0.95 0.05

ANOVA


Between Groups 0.21 3 0.07 1.18 0.3174 2.63


Total 23.44 399



EFFICACY/CROP TOLERANCE PERFORMANCE FORM Page 52 of 69 Tolerance Study AAFC15-068T-200 Indaziflam / Lowbush Blueberry Appendix 2-19: Percent cover; 4 quadrats in each of 4 plots per treatment. Quadrats were 25 cm x 25 cm (625 cm2). August 21 2015

UTC

Alio

n 375 m

L/ha

Alio

n 750 m

L/ha

Velpar 2.22 kg/ha

1 95 90 95 100

2 95 90 95 100

3 70 100 100 95

4 90 90 100 100

Mean 87.50 92.50 97.50 98.75

S.E. 5.95 2.50 1.44 1.25

5 85 90 95 100

6 95 90 90 90

7 95 90 100 100

8 85 95 95 95

Mean 90.00 91.25 95.00 96.25

S.E. 2.89 1.25 2.04 2.39

9 90 95 95 100

10 95 75 90 95

11 90 95 80 95

12 90 90 95 90

Mean 91.25 88.75 90.00 95.00

S.E. 1.25 4.73 3.54 2.04

13 90 100 100 95

14 90 80 80 100

15 95 90 90 90

16 95 85 85 95

Mean 92.50 88.75 88.75 95.00

S.E. 1.44 4.27 4.27 2.04

a a ab b

Trt mean 90.31 90.31 92.81 96.25

Std. Error 0.53 0.47 1.03 0.44

Different letters(a, b) indicate significant differences at the 0.05 level

EFFICACY/CROP TOLERANCE PERFORMANCE FORM Page 53 of 69 Tolerance Study AAFC15-068T-200 Indaziflam / Lowbush Blueberry Appendix 2-20: Stem density as % cover ANOVA; 4 plots. August 21 2015 Anova: Single Factor % cover in a 50 cm x 50 cm quadrat

4 replicates plots, each with 4 subsamples.

SUMMARY


UTC 4 361.25 90.31 4.56 a

Alion 375 mL/ha 4 361.25 90.31 3.52 a

Alion 750 mL/ha 4 371.25 92.81 17.06 ab

Velpar DF 2.22 kg/ha 4 385.00 96.25 3.13 b

ANOVA

Source of Variation SS df MS FP‐

value F crit

Between Groups 94.82 3 31.61 4.47 0.0250 3.49


Total 179.59 15


MS 7.06

r 4 replicates

MS/r 1.77

Sy 1.3289

p 4 treatments

error df 12

q .05 4.20

5.58

*** END OF 2015 DATA ***


UTC Alion 375 mL/ha

15 28 22 27 22 30 22 38

15 23 30 23 22 33 23 39

17 36 31 30 17 30 20 42

19 25 20 28 27 25 19 37

22 30 29 26 22 20 21 44

22 27 22 34 24 25 23 25

19 30 29 33 21 25 24 23

11 26 22 32 22 20 21 23

18 26 28 30 20 22 30 22

17 40 28 30 20 25 21 18

15 24 20 25 20 22 24 21

17 27 19 30 24 27 26 18

16 29 27 27 25 27 29 25

30 27 18 24 20 25 28 20

29 27 15 32 21 29 25 20

27 34 16 28 22 26 25 23

29 32 24 28 25 32 26 28

22 23 20 24 18 29 26 25

22 18 18 22 20 26 25 34

24 23 17 25 16 27 29 31

24 28 28 30 22 27 32 24

25 21 25 31 30 26 30 25

23 31 22 35 29 29 23 22

27 33 30 27 30 28 29 30

23 32 30 27 16 21 27 30

Plot Means 21.12 28 23.6 28.32 22.2 26.24 25.12 27.48

Std. Error 1.02 0.98 1.01 0.69 0.78 0.69 0.70 1.51

Aver. 25.26 Aver. 25.26

S.E. 0.55 S.E. 0.52



25 30 38 25 24 20 25 25

24 28 36 28 32 22 27 18

23 31 47 30 25 24 26 24

15 27 43 28 26 27 28 20

22 28 37 31 25 26 22 21

30 27 37 35 26 28 23 24

23 28 32 22 22 28 26 22

23 19 24 26 30 30 19 26

26 22 23 29 30 35 29 22

23 26 27 30 33 28 23 29

22 27 22 21 26 26 29 15

20 16 24 28 24 25 27 17

26 24 20 28 26 18 22 19

19 31 26 28 33 24 30 20

25 26 22 25 25 26 26 19

27 30 24 29 26 26 26 19

24 16 25 27 18 31 19 17

16 20 25 32 25 27 15 22

22 27 22 24 24 33 22 19

21 27 24 25 24 32 30 22

22 20 23 22 25 27 20 20

25 24 25 30 27 24 22 18

19 25 24 26 27 18 33 22

26 20 30 21 26 24 33 20

22 31 34 34 27 32 36 20

Plot Means 22.8 25.2 28.56 27.36 26.24 26.44 25.52 20.8

Std. Error 0.67 0.90 1.47 0.75 0.67 0.87 0.99 0.62

Aver. 25.98 Aver. 24.75

S.E. 0.54 S.E. 0.46

EFFICACY/CROP TOLERANCE PERFORMANCE FORM Page 56 of 69 Tolerance Study AAFC15-068T-200 Indaziflam / Lowbush Blueberry Appendix 2-23: Stem height (cm) ANOVA; 100 data points. July 05 2016 Anova: Single Factor Stem Height (cm) July 5, 2016

25 values from each of 4 plots per treatment.

SUMMARY


UTC 100 2526 25.26 30.578

Alion 375 mL/ha 100 2526 25.26 27.265

Alion 750 mL/ha 100 2598 25.98 28.989

Velpar DF 2.22 kg/ha 100 2475 24.75 20.917

ANOVA


Between Groups 76.75 3 25.583 0.950 0.417 2.627

Within Groups 10667.19 396 26.937

Total 10743.94 399




UTC Alion 375 mL/ha

19 26 27 31 18 30 25 37

20 26 25 28 18 28 31 37

21 33 22 25 15 27 24 24

18 37 26 27 19 14 16 26

18 31 22 38 26 35 18 32

17 28 19 32 27 39 33 15

24 28 20 38 21 20 29 19

19 28 28 34 23 22 34 14

27 27 25 28 23 25 35 22

25 29 15 26 24 21 21 27

29 25 26 29 24 24 18 17

29 22 28 29 23 27 28 18

19 28 28 32 25 28 23 26

18 24 23 29 26 29 24 25

15 24 22 25 32 24 31 25

31 37 24 24 28 31 34 31

18 25 25 34 26 26 32 36

29 23 22 33 27 32 22 28

28 27 31 33 24 37 30 23

18 30 31 37 22 34 30 23

20 30 27 36 27 27 30 24

25 35 24 29 25 24 26 15

22 37 30 33 24 24 28 17

22 35 25 32 21 28 29 19

12 29 28 38 29 29 28 17

Plot Means 21.72 28.96 24.92 31.2 23.88 27.4 27.16 23.88

Std. Error 1.00 0.90 0.76 0.85 0.76 1.11 1.06 1.37

Aver. 26.70 Aver. 25.58

S.E. 0.57 S.E. 0.57



29 32 39 29 22 24 29 23

26 31 27 26 26 28 29 30

24 29 30 28 21 29 29 25

17 29 28 30 23 26 30 22

24 23 28 32 34 29 24 32

26 21 32 38 30 36 23 25

24 21 29 25 28 32 30 23

26 19 28 27 25 38 22 28

19 29 22 28 26 27 29 16

23 24 32 34 25 29 28 17

24 22 18 34 24 29 29 19

22 26 25 31 22 26 30 20

19 27 29 28 21 27 32 27

21 27 25 25 16 27 32 19

23 27 26 25 18 18 27 14

20 20 23 29 24 23 27 20

19 21 21 27 24 27 20 18

20 26 20 30 30 28 14 18

27 29 21 30 31 24 29 21

21 20 22 31 38 30 22 24

22 23 18 28 27 30 20 22

24 24 22 28 26 34 21 29

29 24 26 28 28 33 27 20

15 34 26 26 26 26 37 26

29 29 25 27 25 24 34 21

Plot Means 22.92 25.48 25.68 28.96 25.6 28.16 26.96 22.36

Std. Error 0.74 0.82 0.97 0.63 0.95 0.86 1.02 0.91

Aver. 25.76 Aver. 25.77

S.E. 0.45 S.E. 0.51



SUMMARY


UTC 100 2670 26.70 32.293

Alion 375 mL/ha 100 2558 25.58 32.105

Alion 750 mL/ha 100 2576 25.76 20.144

Velpar DF 2.22 kg/ha 100 2577 25.77 26.058

ANOVA


Between Groups 76.79 3 25.5958 0.9257 0.4283 2.6274

Within Groups 10949.31 396 27.6498

Total 11026.10 399




UTC Alion 375 mL/ha

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 0 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 0

1 1 0 1 1 1 1 0

1 1 0 1 1 1 0 1

1 1 1 0 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

Plot Means 1.00 1.00 0.92 0.96 1.00 0.96 0.96 0.92

Std. Error 0.00 0.00 0.06 0.04 0.00 0.04 0.04 0.06

Aver. 0.97 Aver. 0.96

S.E. 0.02 S.E. 0.02



1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 0 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 0 1 1 1 1 1

1 1 0 1 1 1 1 1

1 1 0 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 0 1 1 1 1 1

1 1 0 1 1 1 1 1

Plot Means 1.00 1.00 0.80 1.00 1.00 0.96 1.00 1.00

Std. Error 0.00 0.00 0.08 0.00 0.00 0.04 0.00 0.00

Aver. 0.95 Aver. 0.99

S.E. 0.02 S.E. 0.01



SUMMARY


UTC 100 77 0.77 0.179

Alion 375 mL/ha 100 77 0.77 0.179

Alion 750 mL/ha 100 71 0.71 0.208

Velpar DF 2.22 kg/ha 100 84 0.84 0.136

ANOVA


Between Groups 0.85 3 0.282 1.611 0.186 2.627


Total 70.30 399

Calculated F is less than the critical F. There are no significant differences at the 0.05 level.


UTC Alion 375 mL/ha

1 1 1 1 0 1 1 1

1 1 1 1 1 1 1 0

1 1 1 1 1 1 1 0

1 1 1 1 1 0 1 0

1 1 0 1 1 1 1 1

1 1 0 1 1 1 1 0

1 1 0 1 1 1 1 0

1 1 0 1 1 0 1 0

1 1 1 1 1 1 1 1

1 1 0 1 1 0 0 1

1 1 1 1 1 1 0 1

1 0 0 1 1 1 1 0

1 1 1 1 1 1 1 1

0 1 1 0 1 1 1 1

1 1 1 0 1 1 1 1

0 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 0 0 1 1 1 1

1 1 1 1 1 1 1 1

0 0 1 1 1 1 1 1

1 1 1 0 1 1 0 1

1 1 1 1 1 0 0 1

1 1 1 1 1 1 0 1

0 1 1 1 1 1 1 1

Plot Means 0.84 0.92 0.72 0.84 0.96 0.84 0.80 0.72

Std. Error 0.07 0.06 0.09 0.07 0.04 0.07 0.08 0.09

Aver. 0.83 Aver. 0.83

S.E. 0.04 S.E. 0.04



1 1 1 1 1 1 1 1

1 1 1 0 1 1 1 1

1 1 0 1 1 1 1 1

1 1 0 1 1 1 1 1

1 1 1 1 1 1 1 1

1 0 1 1 1 1 0 1

1 0 0 1 1 1 1 1

1 0 0 1 1 1 1 1

1 0 1 1 1 1 1 0

1 1 1 1 1 1 1 1

0 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1

1 0 1 0 1 1 1 0

1 1 1 1 1 0 1 0

1 0 1 1 1 0 1 1

1 0 1 1 1 1 1 1

0 1 1 1 1 0 1 1

1 1 1 1 1 0 1 1

1 1 1 1 1 0 1 1

1 1 0 1 1 1 1 1

1 1 0 1 1 1 0 1

0 1 1 1 1 1 1 1

0 1 1 1 1 1 1 1

1 1 0 1 1 1 1 1

Plot Means 0.84 0.72 0.72 0.92 1.00 0.80 0.92 0.88

Std. Error 0.07 0.09 0.09 0.06 0.00 0.08 0.06 0.07

Aver. 0.80 Aver. 0.90

S.E. 0.04 S.E. 0.03



SUMMARY


UTC 100 83 0.83 0.1425

Alion 750 mL/ha 100 83 0.83 0.1425

Velpar DF 2.22 kg/ha 100 80 0.80 0.1616

Alion 375 mL/ha 100 90 0.90 0.0909

ANOVA


Between Groups 0.54 3 0.1800 1.3393 0.2612 2.6274


Total 53.76 399



EFFICACY/CROP TOLERANCE PERFORMANCE FORM Page 66 of 69 Tolerance Study AAFC15-068T-200 Indaziflam / Lowbush Blueberry Appendix 2-33: Percent cover; 4 quadrats in each of 4 plots per treatment. August 19 2016

UTC

Alio

n 375 m

L/ha

Alio

n 750 m

L/ha

Velpar 2.22 kg/ha

1 90 95 95 95

2 95 95 95 95

3 90 95 95 95

4 95 95 95 95

Mean 92.50 95.00 95.00 95.00

S.E. 1.44 0.00 0.00 0.00

5 95 95 95 95

6 95 95 95 95

7 95 95 95 95

8 95 95 95 95

Mean 95.00 95.00 95.00 95.00

S.E. 0.00 0.00 0.00 0.00

9 95 95 95 95

10 95 95 95 95

11 95 95 95 95

12 95 95 95 95

Mean 95.00 95.00 95.00 95.00

S.E. 0.00 0.00 0.00 0.00

13 95 95 90 95

14 95 95 95 95

15 95 95 95 95

16 95 95 95 90

Mean 95.00 95.00 93.75 93.75

S.E. 0.00 0.00 1.25 1.25

Trt mean 94.38 95.00 94.69 94.69

Std. Error 0.43 0.00 0.31 0.31

EFFICACY/CROP TOLERANCE PERFORMANCE FORM Page 67 of 69 Tolerance Study AAFC15-068T-200 Indaziflam / Lowbush Blueberry Appendix 2-34: % cover ANOVA; 4 quadrats in each of 4 plots per treatment August 19 2016 Anova: Single Factor % cover in 50 cm x 50 cm quadrats: Aug 19 2016

4 quadrats in each of 4 plots per treatment

SUMMARY


UTC 16 1510 94.38 2.92

Alion 375 mL/ha 16 1520 95.00 0.00

Alion 750 mL/ha 16 1515 94.69 1.56

Velpar DF 2.22 kg/ha 16 1515 94.69 1.56

ANOVA


Between Groups 3.13 3 1.042 0.690 0.562 2.758


Total 93.75 63



EFFICACY/CROP TOLERANCE PERFORMANCE FORM Page 68 of 69 Tolerance Study AAFC15-068T-200 Indaziflam / Lowbush Blueberry Appendix 2-35: Blueberry yield (g); 4 quadrats in each of 4 plots per treatment. Quadrats were 50 cm x 50 cm. August 19 2016

UTC

Alio

n 375 m

L/ha

Alio

n 750 m

L/ha

Velpar 2.22 kg/ha

1 12 14 40 14

2 15 15 31 8

3 10 22 44 32

4 25 33 38 15

Mean 15.50 21.00 38.25 17.25

S.E. 3.33 4.38 2.72 5.15

5 24 27 55 43

6 22 45 18 43

7 56 29 29 61

8 39 45 53 38

Mean 35.25 36.50 38.75 46.25

S.E. 7.89 4.92 9.10 5.06

9 18 31 36 15

10 16 38 24 14

11 42 47 22 14

12 39 42 24 30

Mean 28.75 39.50 26.50 18.25

S.E. 6.82 3.38 3.20 3.92

13 25 39 35 26

14 15 15 8 26

15 17 49 11 25

16 23 46 14 30

Mean 20.00 37.25 17.00 26.75

S.E. 2.38 7.71 6.12 1.11

Average 24.88 33.56 30.13 27.13

S.E. 3.19 3.05 3.51 3.53

EFFICACY/CROP TOLERANCE PERFORMANCE FORM Page 69 of 69 Tolerance Study AAFC15-068T-200 Indaziflam / Lowbush Blueberry Appendix 2-36: Yield ANOVA; 4 quadrats in each of 4 plots per treatment August 19 2016 Anova: Single Factor Fruit yield (g) Aug 19 2016

Four (25 cm square) quadrats in each of 4 plots per treatment

SUMMARY


UTC 16 398 24.88 162.917

Alion 375 mL/ha 16 537 33.56 148.796

Alion 750 mL/ha 16 482 30.13 197.183

Velpar DF 2.22 kg/ha 16 434 27.13 199.583

ANOVA


Between Groups 681.42 3 227.14 1.28 0.29 2.76

Within Groups 10627.19 60 177.12

Total 11308.61 63



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