Research Journal of

Chemistry and Environment

Vol. 22 (Special Issue II), August 2018

E-ISSN: 2278 - 4527 ! PRINT-ISSN No. 0972 - 0626

Journal is indexed in SCOPUS andChemical Abstracts

ii

GOD IS.

E-ISSN: 2278 – 4527 PRINT-ISSN No. 0972-0626

RESEARCH JOURNAL OF CHEMISTRY AND ENVIRONMENT

An International Research Journal of Chemical Sciences and Environmental Sciences

Res. J. Chem. Environ., Volume 22(Special Issue II), Pages 1-350, August (2018)

Editor- in- Chief (Hon.)

Dr. D.K. Vardhan Mumbai, INDIA

Correspondence Address:

Research Journal of Chemistry and Environment

Sector AG/80, Scheme No. 54, Indore 452 010 (M.P.) INDIA

Phone and Fax: +91-731-4004000

Website: https://worldresearchersassociations.com

E-mail: info@worldresearchersassociations.com

CONTENTS

Research Papers:

1. Biosynthesis of Silver Nanoparticles using Jicama Extract and Its Application for Colorimetric

Sensing of Mercury Ions

- Firdaus M. Lutfi, Megarani Juwita, Purawiardi R. Ibrahim, Eddy Diana Rakhmawaty and Rahayu Iman

1-3

2. Synthesis and Structural Analysis of Aurivillius Phase, Ca1-xBi3+xNdTi4-xMnxO15

- Zulhadjri, Bilian Sahiga J.I., Wendari Tio Putra and Emriadi

4-9

3. Digital-Based Image Detection System in Simple Silver Nanoparticles-based Cyanide Assays

- Tambaru David, Nomi Anastasia Grandivoriana and Nitti Fidelis

10-14

4. Preparation of Mixed Matrix Polymeric Membrane for removing of contaminants in Crude Biodiesel

- Saiful, Ramli Muliadi, Maulana Ilham, Fadli and Yusuf M.

15-21

5. A new zinc (II) responsive MRI contrast agent

- Adhitiyawarman and Mark P. Lowe

22-30

6. Synthesis of Benzyl-Protected Robtein (2,3,4,4,5-Pentahydroxychalcone) as an Intermediate to (–)-

Robidanol

- Suharti, Achmadi Suminar S., Irfana Luthfan, Sugita Purwantiningsih, Wukirsari Tuti, Ilmiawati Auliya

and Arifin Budi

31-38

7. Antibacterial and antioxidant activities of pyrogallol and synthetic pyrogallol dimer

- Cynthia, Ignatia Florence, Sutanto Hery and Darmawan Akhmad

39-47

8. Preparation and Characterization of Titania/Bentonite Composite Application on the Degradation of

Naphthol Blue Black Dye

- Lubis Surya, Sheilatina, Putra Vicky Praja and Nika Syahrinta Sepia

48-53

9. Optimization of Light Exposure on Superoxide Scavenger Test of Manganese (III)-Salen Acetate

Complex - Nurrahmajanti Asiyah, Onggo Djulia and Deawati Yusi

54-57

10. Application of Molecularly Imprinted Polymer Technique on Chitosan Membranes for Increasing

Creatinine Transport Effectiveness

- Eka Sulistyaningsih

58-64

11. Total Phenolic and Flavonoid Contents and Antimicrobial activity of Acorus calamus L. Rhizome

Ethanol Extract

- Rita Wiwik Susanah, Kawuri Retno and Swantara I. Made Dira

65-70

iii

12. Optimization of process parameters for inulinase production from endophytic fungi Fusarium solani

LBKURCC67, Humicola fuscoatra LBKURCC68 and Fusarium oxysporum LBKURCC69

- Devi Silvera, Illiyin Luthfin, Ardhi Aulia, Pratiwi Nova Wahyu and Saryono

71-78

13. Morphological identification and hydrolytic enzyme-producing abilities of fungi associated with

wilting banana plants (Musa sp.)

- Saryono, Piska Finna, Sari Nurmala, Pratiwi Nova Wahyu and Ardhi Aulia

79-86

14. 20-Epibryonolic Acid from Tetrameles nudiflora Leaves

- Darmawan Akhmad, Fajriah Sofa, Megawati and Lotulung Puspa Dewi N.

87-90

15. Synthesis of fluorescent compound, 7-Hydroxy-4-methyl-2H-chroman-2-one via Pechmann

condensation of citric acid and resorcinol

- Al Anshori Jamaludin, Rahayu Diah Siti, Hidayat Ace Tatang, Hidayat Ika Wiani and Zainuddin Achmad

91-96

16. Distinguishing of Oligosaccharide Linkage Isomers by Nanoparticle-assisted Fragmentation in

MALDI-TOF MS

- Primadona Indah, Dedi, Wang Yi-Sheng and Chen Yu-Ju

97-101

17. Recombinant Anti BNP-SCFV Production in Escherichia coli and its Application for the Detection of

Heart Failure by Electrochemical Immunosensor using Screen-Printed Carbon Electrode-Gold

Nanoparticles (SPCE-GNP)

- Gaffar Shabarni, Hartati Yeni Wahyuni, Susanti Rini and Subroto Toto

102-107

18. Chemical Compositions of Banana Peels (Musa sapientum) Fruits cultivated in Malaysia using

proximate analysis

- Hassan Pyar and Peh K.K.

108-113

19. Isolation and Identification of Quercetin Derivatives and their -Glucosidase Inhibitory Acitivities

from Bryophyllum pinnatum

- Fajriah Sofa, Darmawan Akhmad, Megawati Megawati and Hanafi Muhammad

114-119

20. Influence of synthesis time on Lanthanum Silicate Apatite (La9.33Si6O26) properties

- Noviyanti Atiek Rostika, Juliandri, Agustina Shofia Utari and Malik Yoga Trianzar

120-123

21. A comparison of the fermentation performance and stress tolerance of baker’s yeast cells grown in

media with or without magnesium addition

- Rachman Saadah D., Hidayat Risma W., Safari Agus and Ishmayana Safri

124-128

22. Investigating Aluminasilicate Source in Geopolymer Compressive Strength: Surface Morphology and

Crystal Phase Study

- Anggarini Ufafa and Sukmana Ndaru Candra

129-133

23.

Optimization Density of Geopolymer Concrete based on Taguchi Method

- Sukmana Ndaru Candra, Pambudi Andry Agung and Anggarini Ufafa

134-138

24. Synthesis of 5-(4’-Chlorobenzylidene) hydantoin and N-3 Substituted 5-(4’-Chlorobenzylidene)-3-

Methylhydantoin

- Naufal Muhammad, Hamdani Ulfi Dadan, Maharani Rani, Al Anshori Jamaludin and Hidayat Ika Wiani

139-145

25. In vitro antioxidant activity of Sonneratia ovata Backer extract

- Khumaidah Laili, Nurmalasari Fatati, Putri Fatmawati Inneke, Purnomo Adi Setyo, Santoso Mardi,

Ersam Taslim and Fatmawati Sri

146-150

26. Effects of bacterium Ralstonia pickettii addition on DDT biodegradation by Daedalea dickinsii

- Purnomo Adi Setyo, Rizqi Hamdan Dwi, Fatmawati Sri, Putro Herdayanto Sulistyo and Kamei Ichiro

151-156

iv

27. The effect of Calcination Temperature of Sulfated Zirconia Catalyst for Simultaneous Reactions in

Biodiesel Production

- Andrijanto Eko and Brown Rob

157-162

28. Synthesis of Zirconia 1-D Nanomaterials from Local Zircon-Based Zr(OH)4 mediated by PEG-6000

- Septawendar Rifki, Nuruddin Ahmad, Maryani Eneng, Sutardi Suhanda and Purwasasmita Bambang

Sunendar

163-171

29. Effects of the Precipitation pH of Sodium Silicate on the Amorphous Silica Characteristics and its

capability in the Pb and Cd Adsorption

- Maryani Eneng, Septawendar Rifki and Suhanda

172-178

30. Critical Parameters of Silver Nanoparticles (AgNPs) synthesized by Sodium Borohydride Reduction

- Sholikhah Umi Nur, Pujiyanto Anung, Lestari Enny, Sarmini Endang, Lubis Hotman, Widyaningrum

Triani and Febriana Siska

179-183

31. Antibacterial Activity of Prenylated Xanthones from Pericarp of Garcinia mangostana against

Persistent Dental Infection Microorganism Enterococcus faecalis

- Setiawan Arlette Suzy, Herawati Mieke, Dewi Warta and Supratman Unang

184-188

32. Identification of a DNA Polymerase I Gene Fragment from a Local Isolate (PLS 80) from an

Underwater Hot Spring

- Febriani, Helwati H., Velayati M.A. and Iqbalsyah T.M.

189-192

33. Biohydrogen Production through Separate Hydrolysis and Fermentation and Simultaneous

Saccharification and Fermentation of Empty Fruit Bunch of Palm Oil

- Kusmardini Diah, Prasetyo Joni, Saepudin Endang and Hudiyono Sumi

193-197

34. Study Cytotoxicity of Haarlem Oil by Crystal Violet Staining Assay

- Khairan Khairan, Jamier Vincent and Jacob Claus

198-202

35. Determination of Optimum Pressure for Gd3+ Separation from Gd-DTPA with Nanofiltration

Membrane

- Rahayu Iman, Winanti Anti, Juliandri, Noviyanti Atiek Rostika, Eddy Diana Rakhmawaty, Anggraeni Anni

and Bahti Husein H.

203-205

36. Cytotoxic Effects of Cassava (Manihot esculenta Crantz), Adira-2, Karikil and Sao Pedro Petro

Varieties against P-388 Murine Leukemia Cells

- Widiastuti Diana, Mulyati Ade Heri, Eka Herlina, Siti Warnasih, Suchyadi Yudhie, Triastinurmiatiningsih

and Supratman Unang

206-208

37. Coating of Nanotube Ti-6Al-4V Alloy with Hydroxyapatite-Chitosan Composite by Electrophoretic

Deposition Method

- Charlena, Suparto Irma Herawati and Puspitawati Tuti

209-213

38. pH Effect of Gold Nanoparticles Synthesis Encapsulation in Polyamide Amine Generation 3.0

(AuNP-PAMAM G3.0)

- Pujiyanto Anung, Lestari Enny, Kurniasih Dede, Mujinah, Sholikhah Umi Nur, Gunawan Adang Hardi,

Amalia Sarah S. and Mutalib Abdul

214-219

39. Mode of action of an insecticidal oleanane glycoside on digestive enzymes of Crocidolomia pavonana

- Maharani Rani, Rizqiani Anggun Fuji, Ishmayana Safri, Hidayat Yusup and Dono Danar

220-225

40. Comparative assessment of yeast fermentation performance, ethanol tolerance and membrane fluidity

- Ishmayana Safri, Kennedy Ursula J. and Learmonth Robert P.

226-235

v

41. Preparation of ZnO/SiO2 Composite using Silica isolated from Rice Husk

- Eddy Diana Rakhmawaty, Ishmayana Safri, Noviyanti Atiek Rostika and Wahyuni Astri

236-239

42. Separation of Radioisotope Iodine-131 from Radiotellurium-131 using Dowex-1x8 resin column

chromatography

- Setiawan Duyeh, Nugraha Nabil Aulia and Hastiawan Iwan

240-244

43. Prelimimary Study of using Palm Oil Mill Effluent to produce Bio Hydrogen as Biofuel

- Prasetyo Joni, Febijanto Irhan, Murti S.D. Sumbogo and Dewi Eniya L.

245-248

44. Analysis and Characterization of Complex Compound Gadolinium-(1,4,7,10-Tetraazacyclododecane,

1-4-7-10-Tetraacetic Acid)n-Poliamidoamine Generation 3-Trastuzumab as a Novel Contrast Agent

for Magnetic Resonance Imaging

- Kusuma Yenni Nugraha Wira, Mutalib Abdul, Anggraeni Anni, Ramli Martalena, Fauzia Retna Putri and

Bahti Husein H.

249-254

45. Comparison of Different Methods for Purification of Gold Nanoparticles– Polyamidoamine

(PAMAM) Generation 4 Dendrimer

- Narsa Angga Cipta, Pujiyanto Anung, Gunawan Adang H., Yusup Iyus Maolana, Listyowati Indrarini,

Fauzia Retna Putri, Bratadiredja Marline Abdassah, Mutalib Abdul, Soetikno Ristaniah D. and

Soedjanaatmadja R. Ukun M.S.

255-259

46. Utilization of Sericin on Modification of Cotton Dyeing using Acid Dyes

- Ramadhani Puri Awaliyah and Umam Khairul

260-266

47. Toxicity of Seed oil of Azadirachta indica, Calophyllum inophyllum and their Mixture against

Crocidolomia pavonana Larvae

- Kadarusman Ratna Puji Astuti, Widayani Neneng S., Dono Danar, Hidayat Yusup, Sunarto Toto,

Hartati Sri and Maharani Rani

267-273

48. Addition of mango essence to methyl eugenol more attracted to Bactrocera dorsalis Complex

(Diptera: Tephritidae) on mango plantation in Majalengka, West Java

- Susanto Agus, Djaya Luciana and Sudrajat Rian Aprianti

274-279

49. Solubility Improvement of Antimalaria Drug through Co-Crystalization with Malic Acid

- Gozali Dolih, Alatas Fikri and Sugandi Rani

280-284

50.

Simple method and environmentally friendly ash content removal process from rice husk char using

potassium carbonate solution

- Solihudin, Rustaman and Haryono

285-288

51. Polymer Electrolyte Membrane Fuel Cell based on Sulfonated Polystyrene and Phosphoric Acid with

Biocellulose as a Matrix

- Fadlinatin Naumi, Christi L. Natanael, Iman Rahayu, Lucia Indrarti and Sunit Hendrana

289-293

52. A Voltammetric Immunosensor for Detection of HER2 using Gold Modified-Screen Printed Carbon

Electrode

- Hartati Yeni Wahyuni, Beladona Siti U. Misonia, Wyantuti Santhy and Gaffar Shabarni

294-301

53. Fast and Simultaneous Detection of Sm, Eu, Gd, Tb and Dy using combination of Voltammetry

Method and Multivariate Analysis

- Wyantuti Santhy, Pratomo Uji, Hartati Yeni Wahyuni, Anggraeni Anni and Bahti Husein H.

302-306

54. Vannamei Shrimp (Litopenaeus vannamei, Boone, 1931) Performance with Bacillus and Lactobacillus

Probiotic Formulation Consortium in Dry Preparation

- Andriani Yuli, Safitri Ratu and Fauziah Shaiyanne

307-312

vi

55. The Implementation of Natural Pigments of Pumpkin Meal to enhance the Color Quality of Koi Fish

(Cyprinus carpio)

- Yustiati Ayi, Arisya Yuni, Herawati Titin, Rachman Boedi and Andriani Yuli

313-318

56. Novel Mechanical Filter for reducing Ammonia Concentration of Silver Barb culture in a

Recirculating Aquaculture System (RAS)

- Hasan Zahidah, Rajibbusalam, Maulina Ine and Andriani Yuli

319-324

57. The Reduction of Lead in Fish Meat from Floating Net Cages in Cirata Reservoir by Citric Acid

- Junianto, Hasan Zahidah and Afrianto Eddy

325-329

58. Cytotoxic Sesquiterpenoid from the Stembark of Aglaia argentea (Meliaceae)

- Harneti Desi, Farabi Kindi, Nurlelasari, Maharani Rani, Supratman Unang and Shiono Yoshihito

330-333

59. Cellulose isolation from Gracilaria Genus and its Potential as Bioethanol Raw Material

- Satiyarti Rina Budi, Febrishaummy Indriani, Yun Yenny Febriyani and Mulyani Rahmaniar

334-336

60. Sn (IV) doped lanthanum silicate apatite structure (La9.33Si6-xSnxO26; x: 0.1; 0.3; 0.5) as an electrolyte

- Noviyanti Atiek Rostika, Eddy Diana Rakhmawaty, Hastiawan Iwan, Dzulfikar Muhammad

and Syarif Dani Gustaman

337-341

61. Determination of Individual Spectra of Sm, Eu, Gd, Tb and Dy from the UV-Vis Spectrum of

Mixture Solution

- Anggraeni Anni, Avian Mohamad Sepril, Purnama Andrew and Mutalib Abdul

342-346

62. Secondary Metabolites from Steambarks of Dysoxylum alliaceum

- Mayanti Tri, Nurcahyanti Ois, Darwati, Julaeha Euis, Farabi Kindi, Sumiarsa Dadan

and Dinata Deden Indra

347-350

Research Journal of Chemistry and Environment_______________________________Vol. 22(Special Issue II) August (2018) Res. J. Chem. Environ.

255

Comparison of Different Methods for Purification of Gold Nanoparticles– Polyamidoamine (PAMAM)

Generation 4 Dendrimer Narsa Angga Cipta1,2*, Pujiyanto Anung3, Gunawan Adang H.3, Yusup Iyus Maolana4, Listyowati Indrarini4,

Fauzia Retna Putri1, Bratadiredja Marline Abdassah5, Mutalib Abdul1, Soetikno Ristaniah D.4

and Soedjanaatmadja R. Ukun M.S.1

1. Department of Chemistry, Faculty of Mathematics and Science, Universitas Padjadjaran, Jatinangor, Sumedang, West Java, INDONESIA

2. Pharmaceutical Research and Development Laboratory of FARMAKA TROPIS, Faculty of Pharmacy, Mulawarman University, Samarinda,

East Kalimantan, INDONESIA

3. Center of Radioisotopes and Radiopharmaceuticals, National Nuclear Energy Agency, Serpong, Tanggerang, INDONESIA

4. Department of Radiology, Faculty of Medicines, Universitas Padjadjaran, Bandung, West Java, INDONESIA 5Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor, West Java, INDONESIA

*anggaciptan@yahoo.com

Abstract Gold nanoparticles (AuNP) are currently being widely

applied in cancer diagnosis as a CT Scan contrast

agent (imaging) as well asin therapy (theragnostic

agents). The polyamidoamine (PAMAM) G4

dendrimer has also been applied as a stabilizer agent

to control the size of gold nanoparticles. In the

production of AuNP-PAMAM G4 dendrimer, a solvent

and reducing agent were used. Therefore, it is

necessary to separate and purify the synthesis result of

AuNP-PAMAM G4 dendrimer from the undesired

compounds.

Separation and purification were carried out by two

different methods, namely, size exclusion column

chromatography Sephadex G25® (SE), and membrane

ultrafiltration (MU). The percent yield of AuNP that

has been synthesized was calculated by using

Inductively Couple Plasma Atomic Emission

Spectroscopy (ICP-OES) and then characterized by

using UV-Vis Spectroscopy and Transmission

Electron Microscope (TEM). The percent yield of

AuNP, which was carried out by MU, was 93.75%.

This result was better compared to using SE (81.18%).

The characteristics results from the UV-Vis spectrum

demonstrated similar spectra between separation and

purification methods using both MU and SE. The

characteristics results from TEM also showed similar

morphological results.

Keywords: Contrast agents, CT-Scan, dendrimer, gold

nanoparticles, purification.

Introduction The rapid development of nanotechnology has recently led

to research on the synthesis or manufacture of therapeutic

agents as well as the diagnosis (theragnostic) on the

nanoscale, one of which involves gold nanoparticles. The

gold nanoparticles were used as theragnostic agents,

attributable to their advantages over the conventional one

(iodine). Gold nanoparticles have higher atomic numbers

and electron densities and are not toxic in a large

concentration range, and their gold surface is easily modified

for targeted delivery1-3. The technology used in the

production of gold nanoparticles often involves top-down

and bottom-up methods. The bottom-up method involves a

stabilizer to prevent aggregation, and as a template for the

growth of nanoparticles4.

In the production of gold nanoparticles, tetrachloro auric

acid (HAuCl4) is reduced by sodium borohydride (NaBH4)

and PAMAM G4 dendrimer as a stabilizer agent for growing

the template and controlling the particle size of the gold

nanoparticles. Tetrachloroauric acid was produced by

dissolving Au foil using aqua regia/ HCl: HNO3 (3:1)6. The

excess of organic solvents and reducing agents must be

removed by using some method of separation and

purification such as size exclusion column chromatography

(SE) and membrane ultrafiltration (MU).

This work involves the comparison of two purification

methods of gold nanoparticles PAMAM G4 using SE and

MU. The optimal yield will be a consideration to determine

the most suitable method for this purification. Furthermore,

gold nanoparticles PAMAM G4 dendrimer can be used

safely as a cancer theragnostic agent and this approach can

be used as a prototype for the manufacture of a larger

production scale.

Material and Methods Materials: Au foil was purchased from PT. Antam

(Indonesia), and all chemicals were obtained from Aldrich

(USA) and Merck (Germany).

Preparation of HAuCl4 solution: Au foil was dissolved in

aqua regia (HCl: HNO3 = 3:1). The solution was stirred and

heated at 100°C (kept not to burst), and then dissolved with

demineralized water. The dissolution with demineralized

water and heating process was performed three independent

times. Finally, HAuCl4 was dissolved in 0.01 M HCl

solution.

Synthesis and characterization of AuNP-PAMAM G4

dendrimer: The prepared HAuCl4 solution (0.635 mL) was