Research Literature Pack RL 1 Lit A4

Research

Precision Instrumentation for Life Science Research

Volume Flow Measurements

Pressure & PV Loops

Telemetry

Tissue Perfusion

Courtesy of: Microsurgery Research and Training Laboratory, Dept. of Orthopedic Surgery, Columbia U

AMERICASTransonic Systems Inc.34 Dutch Mill RdIthaca, NY 14850U.S.A.Tel: +1 607-257-5300Fax: +1 [email protected]

EUROPETransonic Europe B.V.Punterweg 316222 NW MaastrichtThe NetherlandsTel: +31 43-407-7200Fax: +31 [email protected]

ASIA/PACIFICTransonic Asia Inc.6F-3 No 5 Hangsiang RdDayuan, Taoyuan County33758 Taiwan, R.O.C.Tel: +886 3399-5806Fax: +886 [email protected]

JAPANTransonic Japan Inc.KS Bldg 201, 735-4 Kita-AkitsuTokorozawa Saitama359-0038 JapanTel: +81 04-2946-8541Fax: +81 [email protected]

Research

www.transonic.com

Transonic Systems Inc. is a global manufacturer of innovative biomedical measurement equipment. Founded in 1983, Transonic sells gold standard transit-time ultrasound flowmeters and monitors for surgical, hemodialysis, pediatric critical care, perfusion, interventional radiology and research applications. In addition, Transonic provides pressure and pressure volume systems, laser Doppler flowmeters and telemetry systems.

VOLUME FLOWMake absolute volume flow measurements with Transonic Gold Standard Research Flowmeters. Whether used to measure arterial or venous blood flow in mice to calves with versatile acute/chronic perivascular Flowprobes, or to quantify flow in laboratory tubing, Transonics ultrasonic transit-time Flowmeters are reliable, easy to use and referenced worldwide as the system of choice for research applications. There is a Probe or Sensor to fit every flow application.

PRESSURE & PRESSURE-VOLUMEScisense high fidelity pressure catheters span the entire range of animal sizes from mice to cows. Minimally invasive ADVantage System pressure-volume catheters provide cardiac researchers the unique ability to measure almost every imaginable hemodynamic and contractile index of ventricular function with a single instrument. It is the only catheter-based system to accurately quantify true ventricular volume in real time.

TELEMETRYFully implantable EndoGear biotelemetry system is customizable to meet specific experimental requirements and can measure Doppler flow velocity, pressure, ECG and temperature from a single implant. PhysioGear pocket telemetry system for large animals measures volume flow with transit-time perivascular Flowprobes.

Advancing Meaningful Measurements

TISSUE PERFUSIONLaser Doppler technology measures microvascular blood flow, mass and velocity as a relative indicator of tissue perfusion. A range of surface and needle style Probes cater to specific application needs.

RL-1-fly Rev. A 12/12

RL-52-ds-A4 Rev.B 12/12

Volume Flow

Selection of compatible perivascular Flowprobes

TS420 Flow Module requires a T400 Console in order to take measurements. Consoles can hold multiple Modules of various functionality.

T403 Console with TS410 and TS420 Modules

Transonic TS420 Perivascular Flow Modules provide unprecedented waveform quality and resolution. They incorporate plug and play features with virtually maintenance-free construction for the perfect combination of sophisticated and simple. TS420 Flow Modules and compatible Flowprobes measure absolute blood flow in arteries, veins & ducts for acute and chronic cardiovascular research applications.

UNLIMITED APPLICATIONS Small Animal Cardiovascular; Even Mice!

Renal Hypertension Studies

Carotid Occlusion Thrombosis Models

Large Animal Cardiovascular

Exercise Physiology, Heart Failure Models, Pregnancy Research, Myocardial Ischemia & Reperfusion

VAD Implantation & Heart Assist Models

Novel Species: Comparative Physiology

COMPATIBLE FLOWPROBES (0.5 - 36 MM) PR/PS/V-Series Flowprobes & Nanoprobes

PAU-Series COnfidence Flowprobes for Cardiac Output and great vessels

PMP-Series - Handle style Flowprobes for acute intraoperative applications

TS420 Perivascular Flow ModulePrecision Blood Flow Measurements for In Vivo Animal StudiesGold Standard Ultrasonic Transit-Time Technology

TS420 Perivascular Flow Module Specifications

www.transonic.com

GENERAL FEATURESSize 5.125 h x 4 w x 9.062 d

Weight: 2.2 lbs.

Module fits 2 Console slots (20HP) in T402 or T403 Consoles

Power: Derives input power from 400-Series Consoles. Installation in a Console is required.

OPERATIONAL TECHNOLOGYUltrasonic Transit-time

FLOWPROBE COMPATIBILITYPS-, V-, PR-, PMP- & PAU-Series

PROBE CONNECTORFront panel 10-pin connector. Accepts research perivascular Flowprobes and extension cables with male CRA 10-pin connectors.

AUTOMATIC ADJUSTMENTSProbe size identification and corresponding flow output ranges. Volume flow calibration of applied Probe.

DIGITAL DISPLAY4-Digit (14 segment) LED displays Flow / Probe data / Error Messages

Bar Indicator Light: Displays received signal amplitude for continuous monitoring of Probe signal quality

ANALOG DISPLAYTaut-band needle (-0.2 volts to +1.2 volts ) displays Flow / Received Signal Amplitude/Calibration information

ZERO ADJUSTRecessed momentary push button to zero flow reading at stopped flow

MODE BUTTON CONTROLSSelect modes of operation of displays and outputs

MEASURE MODE: Displays current volume flow values. Indicator light bar shows received signal strength

TEST MODE: Displays Probe size & received signal amplitude

ZERO MODE: Sets outputs to zero flow to calibrate external recording device

SCALE MODE: Sets outputs to scale factor flow to calibrate external recording device

LOW RANGELow Range increases flow gain by a factor of 4

FILTER PROPERTIES 0.1, 10, 40 Hz: 2nd order

Butterworth, with a third passive pole at 160 Hz

160 Hz: 3rd order Butterworth

INVERTInvert the polarity of analog flow outputs and flow displays

SIGNAL OUTPUTS8 accessible signals via 400-Series Flowmeter Consoles back-panel terminal block: Pulsatile Volume Flow; Mean Volume Flow, Received Signal Amplitude (2); Phase (4)

ULTRASONIC FREQUENCY RANGE600 KHz to 14.4 MHz; Probe size dependent.

FLOW OUTPUTFront panel mounted BNC output connector & rear panel terminal block:

Pulsatile/Average Volume Flow

Filtering controlled by front panel selectable filters

Voltage range: -5 to + 5 volts

Output resistance: 500 Ohm

Full Range for Flow: -5 to +5 V (bidirectional flows, with range of 5 x scale factor flow)

AUTOMATIC DIGITAL PROBE ID & CALIBRATIONTS420 circuitry reads Probe operational data (size, scale & calibration) programmed in the probes EPROM.

Probes with 10 or 12-pin connectors: EPROM installed in the connector.

4-pin Probe connectors require separate EPROM keys

CAL KEY PORT: Front panel socket accepts 3-pin EPROM calibration keys for Flowprobes with 4-pin connectors. Flowmeter reads and uses CAL KEY data if both a separate calibration key and connector EPROM are present.

SYNCHRONIZATIONRear panel jumpers select synchronization mode

Self-Triggering Mode: SYNC IN to SYNC OUT jumper on each Module

Sequential Triggering Mode: SYNC IN crossed to SYNC OUT between Modules

RL-54-ds-A4 Rev.B 12/12

Volume Flow

ME-PXN Inline Flowsensor and ME-PXL Clamp-on Flowsensor on tubing

Whether you require tubing flow measurements in the animal lab or in the R&D engineering suite, Transonics TS410 Tubing Flow Module gives you precision volume flow measurements in tubing with user friendly features applicable to laboratory bench settings, in vitro and extracorporeal use.

True Volume Flow

Measurement Capability for Diverse Fluids: Blood, Saline, Water, Cell Culture, Physiological Buffers, Blood Analogs such as Glycerine/Water Solutions

Programmable Alarm Conditions:

High / Low Flow Threshold Alarm Bubble / Received Signal Alarm

On-site recalibration of Flowsensors for new parameters or conditions

ALL TYPES OF FLOW CIRCUITS Circulatory support device development (VAD

Performance & Artificial Heart)

Isolated heart and perfused organ studies

Mock circulatory models

Non-contact sterile flow measurement of biologicals and bioreactors

Flow phantoms and any experimental, or non-human clinical or process application where flow measurement in tubing is needed.

COMPATIBLE FLOWSENSORS ME-PXN Inline Flowsensors

ME-PXL Clamp-on Sterile Tubing Flowsensors TS410 Flow Module requires a T400 Console in order to take measurements. Consoles can hold multiple Modules of various functionality.

T403 Console with TS410 and TS420 Modules.

TS410 Tubing Flow ModulePrecision Volume Flow Measurements for In Vitro & Extracorporeal TubingGold Standard Ultrasonic Transit-Time Technology

www.transonic.com

TS410 Tubing Flow Module SpecificationsGENERAL FEATURESSize: 5.125 h x 4 w x 9.062 d

Weight: 2.3 lbs.

Module fits 2 Console slots (20HP) in T402 or T403 Consoles

Power: Derives input power from Transonic 400-Series Consoles. Installation in a Console is required.

OPERATIONAL TECHNOLOGYUltrasonic Transit-time

FLOWSENSOR COMPATIBILITYME-PXL- & ME-PXN-Series

SENSOR CONNECTORFront panel 16-pin connector. Accepts research Inline and Clamp-on Flowsensors and extension cables with male CC16 or CP16 connectors.

AUTOMATIC ADJUSTMENTSSensor size identification and corresponding flow output ranges. Volume flow calibration and serial number displayed of active Flowsensor.

DIGITAL DISPLAY4-Digit (14 segment) LED displays Flow / Sensor data / Error Messages

Bar Indicator Light: Displays received signal for continuous monitoring of Sensor signal quality.

LCD DISPLAYOne line 16-character alpha numeric LCD displays program parameters, Sensor and Meter status, alarm settings. Default displays Sensor serial number.

SET-UP/STATUS & PROGRAM PARAMETERSSTATUS MODE: (White labels) Status message displayed on LCD.

Sensor Status: sensor type & calibration

Meter Status: Active flowmeter settings & alarm status

Alarm Mute: Audible alarm On/Off

PROGRAM MODE: (Blue labels)

Sensor Controls: Select pre-programmed factory calibration options; Adjust Flowsensor gain to change calibration on-site.

1/4 Flow Scale: increases flow gain by factor of 4 for low flow measurements.

Calibrate Scale: sets output to 0 and 1 Volt to calibrate external recording devices with scale factor flow.

Invert flow: inverts polarity of analog outputs & flow display

Alarms Menu: 3 level program to select, set thresholds, and activate Alarms for Low Flow, High Flow and Received Signal Interruption

FILTER PROPERTIES 0.1, 10, 40 Hz: 2nd order

Butterworth, with a third passive pole at 160 Hz

160 Hz: 3rd order Butterworth

ZERO FLOW ADJUSTRecessed momentary push button to zero flow reading at stopped flow.

FLOW OUTPUTFront panel mounted BNC output connector & rear panel terminal block:

Pulsatile/Average Volume Flow

Filtering controlled by front panel selectable filters

Voltage range: -5 to + 5 volts

Output resistance: 500 Ohm

Full Range for Flow: -5 to +5 V (bidirectional flows, with range of 5 x scale factor flow)

AUTOMATIC DIGITAL SENSOR ID & CALIBRATIONTS410 reads operational data (size, scale & calibration) programmed in the sensors EPROM.

ULTRASONIC FREQUENCY RANGE600 KHz to 14.4 MHz; Sensor size dependent

SIGNAL OUTPUTS8 accessible signals via 400-Series Flowmeter Consoles back-panel terminal block: Pulsatile Volume Flow; Mean Volume Flow; Received Signal Amplitude (2); Phase (4)

SYNCHRONIZATIONRear panel jumpers select synchronization mode

Self-Triggering Mode: SYNC IN to SYNC OUT jumper on each Module

Sequential Triggering Mode: SYNC IN crossed to SYNC OUT between Modules

For Use With TS420 Flowmeter Modules

Volume Flow

Precision Perivascular Flowprobes

Absolute volume flow data from mice to large animals

Acute use and implantable Flowprobes for arteries, veins and ducts

Multiple configurations to meet experimental needs of many animal models

Unparalleled accuracy & signature high resolution flow waveforms

PS-SERIES INCLUDING NANOPROBES0.5-1.5 mm Nanoprobes are scaled to fit mouse anatomy for acute or chronic use.

2-20 mm Flowprobes offer the greatest diversity of customizable features for the perfect fit in any application.

PR-SERIES1 & 1.5 mm Flowprobes for small acute or chronic applications where a more robust design than the Nanoprobes is needed.

PAU-SERIES8-36 mm COnfidence Flowprobes with Ultrafit Liners for ascending aorta and pulmonary artery cardiac output studies.

PMP-SERIES2-14 mm Handle Flowprobes for intraoperative measurements in preclinical animal trials where devices that match those used in clinical settings are preferred.

V-SERIES0.5 & 0.7 mm Microcirculation Flowprobes for acute use only. Larger body and more robust than Nanoprobes. Requires more acoustic gel for larger lumen.

Volume Flow

PS- Nanoprobes

PS-Series

PR-Series

PMP-Series

V-Series

PAU-Series

RL-26-fly-A4 Rev. E 12/12

Volume FlowVolume Flow

Ordering PS-, PR-, PAU-, PMP- & V-Series Flowprobes

Catalog #: MA - Size Series

Examples:

MA-2PSS (Acute use 2 mm PS-Series Probe with side cable exit)

MA-0.5PSB (Acute use 0.5 mm PS-Series Nanoprobe with back cable exit)

MA-20PAU (Acute use 20 mm PAU-Series Probe)

Catalog #: MC - - - - - - Size

Application:

Examples:

MC-1.5PSL-JN-WC04-CA4S-GC (Custom 1.5 mm PS-Series Probe with lateral cable, J reflector, 4 cm cable, CA4S connector and chronic calibration)

MC-20PAU-WC60-CRS10-GAC (Custom 20 mm PAU-Series Probe with 60 cm cable, CRS10 connector and acute/chronic calibration)

Cable Orientation

Series Cable Orient.

Reflector Cable Length

Connector Calibration

Custom Configured FlowprobesCustom configured Probes are available for most Probe series to meet individual application and implant needs. Contact Customer Service for species/vessel recommendations or configure Probes from the table codes. Please note intended application for confirmation when ordering custom Probes as this will assist with determining the most ideal configuration. Custom Probes cannot be exchanged.

Standard Acute Use FlowprobesAll MA- Flowprobes come with the standard maximum cable length, CRA10 connector, and acute calibration (acute/chronic calibration for PAU-Series)

* Cable orientation and reflector style available for PS- & PR-Series only

* Cable orientation available for PS- & PR-Series only

Extension Cable

Cable Length

Cable Orientation*

Reflector Style*

Probe Size & Series

* *

*

MRI COMPATIBILITYTransonic Flowprobes can be made from nonmagnetic components for flow measurements during MRI. Contact Customer Service to special order: ceramic or brass reflector, nonmagnetic connector, Probe ID key for use with extra long extension cables.

Connector

PhysioGear

Species, Vessel

B- Back: perpendicular to vessel

S- Side: parallel to vessel

L- Lateral: for thoracotomy

Custom Ordering of Perivascular Flowprobes

CABLE ORIENTATION*

CODE ORIENTATION PS-SERIES PR-SERIES

B Back 0.5 - 20 1 - 1.5

S Side 0.5 - 20 1 - 1.5

L Lateral 0.5, 1.5 - 2.5 N/A

REFLECTOR STYLES FOR SPECIAL APPLICATIONSMA- STANDARD

CODE DESCRIPTION PS-SERIES PR-SERIES

LS L w/ slide cover 3 - 20 N/A 3-20PS

LSF L w/ slide & silicone flange 3, 4, 6 N/A

JN J w/o slide 0.5 - 16 1 - 1.5 1.5PSL

JFH J w/o slide w/ flexi-handle 0.5 - 2.5 1 - 1.5 0.5, 0.7PS

JS J w/ slide cover 2 - 16 1 - 1.5 2-2.5PS, 1-1.5PR

JSF J w/ slide & silicone flange 2, 2.5 N/A

U U reflector 3 - 20 N/A

USW U w/ wide silicone shield 6 - 20 N/A

USN U w/ narrow silicone shield 6 - 20 N/A

MRI non-magnetic reflector 2 - 20 N/A

STANDARD MAX CABLE LENGTH

CODE DESCRIPTION PS-SERIES PR-SERIES PAU-SERIES

WCxx Up to 60 cm Nanoprobes 1 - 1.5

WCxxx Up to 100 cm 2 - 20* N/A

WCxxx Up to 200 cm N/A N/A 8-36*

CONNECTORS SERIES

CODE DESCRIPTION ACUTE/CHRONIC ANIMALS ID KEY REQUIRED PS- / PR- PAU-

CRA10 10-pin Redel acute connector Acute All No all sizes

CRS10 10-pin Redel sealed connector w/ cap ChronicDog & larger No all sizes

CS12 12-pin titanium connector w/ cap ChronicDog & larger No 1 - 20

all sizes

CB12 12-pin titanium skin button w/ cap ChronicDog & larger No 1 - 20

all sizes

CM4B 4-pin mini connector w/ cap & straight cable ChronicRat, Rabbit & larger Yes all sizes N/A

CM4S 4-pin mini connector w/ cap & right angle cable ChronicRat & Rabbit Yes all sizes N/A

CA4B 4-pin micro connector w/ straight cable ChronicRat & Mouse Yes 0.5 - 10 N/A

CA4S 4-pin micro connector w/ right angle cable ChronicRat & Mouse Yes 0.5 - 10 N/A

CALIBRATION SERIES

CODE DESCRIPTION PS- / PR- PMP- / V- PAU-

GA Acute calibration all sizes

GC Chronic calibration all sizes N/A

GAC Acute/Chronic calibration N/A N/A all sizes

GX Custom calibration all sizes N/A

-P PhysioGear normalization 1-20 N/A all sizes

SILICONE FLANGE (SF) FOR CORONARY IMPLANTSMesh-reinforced silicone flange cemented around the perimeter of Probe is sutured to the myocardium to stabilize the Probe on the heart in LAD artery application; also used in umbilical artery (fetal sheep) application. Can also be used to hold coupling gel in place during acute experiments and to keep the Probe aligned on the beating heart.

SILICONE SHIELDS (USW & USN)U-reflectors on PS-Series Probes may be silicone encapsulated for chronic implant. Cushions vessel for ascending aorta and pulmonary artery implant. Stabilizes Probe on collapsible vessels (i.e. portal vein); keeps fat from infiltrating Probe.

*Determined by anatomy and surgical approach.

Wide Silicone Shield (USW) Narrow Silicone Shield (USN)

L reflector with slide

J reflector with slide

U reflector

Probe with Silicone Flange (SF)

*Substitute xx for shorter lengths

PROBE ID KEY FOR 4-PIN CONNECTORSFlowprobes configured with 4-pin connectors require Probe ID Keys. Probe scale, signal normalization and calibration gain are programmed into the serialized EPROM key. Keys plug into the port of the TS420 Module during Probe use.

CM4B (STRAIGHT) & CM4S (RIGHT ANGLE) 4-PINImplantable plastic mini connectors come with a threaded cap with suture hole, sealing gasket, and Probe ID key. Use with 4-pin extension cables. CM4B has a straight cable entry to ease tunneling subcutaneously and can be stored in an animal jacket pocket when not in use. The CM4S connector has a right angle cable entry and is used with silicone or rigid cuffs to stabilize connector in mid-scapular region. Can be used for most species including: rats, rabbits, and dogs.

CA4B (STRAIGHT) & CA4S (RIGHT ANGLE) 4-PINMinimal impact micro connectors, the 4-pins terminate the Probe cable with a bare minimum of housing. Use with 4-pin extension cables. Comes with Probe ID key. CA4B (straight cable entry) can be threaded into spring stock for use with electrical swivels. CA4S connectors have a right angle cable entry and can be stabilized in the mid-scapular region with a rigid or silicone cuff. For rats and mice.

CRA10 ACUTE REDELMA- Standard for Acute Use Probes: Plugs directly into Flowmeter or extension cable.

CRS10 SEALED REDELImplantable plastic connector has conical threaded cap with suture hole for easy subcutaneous tunneling. Cap contains sealing gasket. Plugs directly into Flowmeter or extension cable.

CS12 SEALED TITANIUMSealed Titanium Connector with pointed cap. Connector includes the Flowprobe calibration EPROM.

CB12 TITANIUM SKIN BUTTONFor long term implantation with any style Probe. The Probe ID EPROM is integrated in the connector. The connector comes with a flat Delrin cap. A pointed cap (CB12-PC) can also be ordered separately. Use with 12-pin extension cables. For dogs, pigs and other large animals.

CONNECTOR DIMENSIONS IN MM

CODE HEIGHTCAP DIAMETER

SHANK DIAMETER

FLANGE DIAMETER

CRA10 44.5 N/A 14 N/A

CRS10 40.3 15.3 11.1 N/A

CS12 32 11.5 9.4 N/A

CB12 21.5 11.5 9.4 18.3

CM4B 15 8 8 N/A

CM4S 15 8 8 N/A

CA4B 10 N/A 4.1 N/A

CA4S 10 N/A 4.1 N/A

Connectors & Accessories for Acute & Chronic Precision Perivascular Flowprobes

CALIBRATION INFORMATIONTransonic Precision Perivascular Flowprobes are each precalibrated for absolute blood flow measurement at 37C using a gravity-fed constant flow setup with water (see RL-5-tn). We calibrate Probes for their intended use to compensate for the different acoustic conditions when using an acoustic gel in acute applications versus fibrotic tissue infiltration that stabilizes the Probe in a chronic implant. Probes calibrated for acute use are calibrated to read 10% higher than Probes for chronic implant (Except PAU-Series with acute/chronic calibration). Sub-acute applications (12 days) more closely mimic an acute preparation and should be calibrated for such. Acute calibration is also recommended for Probes that are used for both acute and chronic application since acute measurements are more often interpreted in absolute terms rather than relative changes in flow over time. Custom calibration for atypical use is available (ex: for fish at 10C).

CRS10

CRA10

CS12

CB12

CA4B

CA4S

PHYSIOGEAR COMPATIBILITYTransonic Flowprobes (1-28 mm) are compatible with PhysioGear pocket telemetry system for large animals. Contact Customer Service for Flowprobe recommendations for all applications.

CM4BCM4S

EXTENSION CABLES

CATALOG # TYPE DESCRIPTION

CRA10-S-CRA10 10-pin 1.25 meter

CRA10-M2-CRA10 10-pin 2 meter (not for use with nanoprobes)

CRA10-M3-CRA10 10-pin 3 meter (not for use with nanoprobes)

CRA10-X-CRA10 10-pin Custom length for MRI

CM12-S-CRA10 12-pin 1.8 meter

CM12-X-CRA10 12-pin Custom length

CM4-S-CRA10 4-pin 1.8 meter with spring

CM4-M3-CRA10 4-pin 3 meter (not for use with nanoprobes)

CM4-X-CRA10 4-pin Custom length; no spring

CA4-S-CRA10 4-pin 1.8 meter; mouse cable; no spring

CODE DESCRIPTIONCOMPATIBLE CONNECTOR

HEIGHT (MM)

DIAMETER (MM)

AAPC102 Silicone cuff CM4S 18.9 5.4

AAPC103 Silicone cuff CA4S 15 5.4

AAPC104 Rigid cuff CM4S 19 7.1

AAPC105 Rigid cuff CA4S 9 3.7

Soft silicone cuffs aid stabilizing mini (CA4 & CM4) connectors for short term implant. Suture holes in base of flange allow attachment on top of skin. Supplied in packages of 10.

Delrin rigid cuffs convert mini connectors to skin buttons for small animal use. Suture holes allow attachment on top of skin; set screw holds connector in place. Robust for long-term or multiple use.

ACOUSTIC COUPLANT FOR ACUTE APPLICATIONSAcoustic couplant gel is used to displace the air between the Flowprobe and the vessel during acute application because air will block transmission of the ultrasound signal. Appropriate coupling gels for use with Transonic Flowprobes have acoustic properties at various temperatures that closely match blood. See RL-9-tn for complete listing.

Surgilube: (# ZC510 4.25 oz. tube) Bacteriostatic Surgical Lubricant from E. Fougera & Co.

NALCO 1181 (# APNALCO 6 oz.) Superabsorbant powder to thicken Surgilube gel for long experiments. For terminal use only.

PROBE & CONNECTOR STABILIZATION PRODUCTSMERSILENE MESH (APMERSILENE): 6 x 6 square soft interlocked polyester fiber mesh implanted under connector fortifies thin skin for cuff or button sutures.

SILICONE WRAPPING STRIP (APWRAP): Thin silicone strip reinforced with mesh wraps around outside of a Probe to stabilize the Probe on a vessel. Provides additional suture points and keeps fat from infiltrating the Probe over long implants.

PROLENE MESH (APPROLENE) & ULTRACELL SPONGE (ASPONGE): Used in chronic implant to protect the ascending aorta against long-term erosion. For use with Probes without full liners (PAX & 32, 36PAU).

Cuffs for Chronic Probe Implants

Connectors & Accessories for Acute & Chronic Precision Perivascular Flowprobes

AAPC103

AAPC105

AAPC104

AAPC102

APMERSILENE

Nanoprobes require 185 cm total cable length (probe + extension cable) to meet specification.

CRA10 Connector

EPROM ID Key

CM4S 4-pin Connector

CM4-S-CRA10 1.8 m Extension Cable with Spring

2.5PSL Probe

Silicone Wrap sutured around Flowprobe





www.transonic.com



CLEANING & STERILIZATIONFollow the directions included with each Flowprobe (I1041).

CUSTOMER SERVICEContact your local distributor or sales representative for quotations, shipping information and payment terms. Please feel free to contact us if you have questions or visit our website www.transonic.com for the most up-to-date product offerings, applications and technical support, and reference materials.

LIMITED WARRANTYTransonic Systems Inc. warrants that perivascular Flowprobes are free from defects which are the result of faulty material or workmanship by Transonic Systems Inc. for a period of six (6) months (Nanoprobes: 3 months) from their date of shipment. The warranty of Transonic shall not apply to: defects caused by abuse, neglect or misuse; damage due to accident or casualty; or un-authorized alterations or repairs made by anyone other than Transonic Systems Inc.

Transonic Systems Inc. will, at no charge to the user, either repair or replace a defective Flowprobe during its warranty period. The Buyer pays shipping charges to Transonic Systems Inc. Transonic will pay for the return shipping charges. No other warranty oral or written, expressed or implied. Transonic is not liable for incidental or consequential damages. Warranty is valid only if equipment is purchased through Transonic or a duly appointed distributor or licensed representative.

CALIBRATION CERTIFICATION & REPAIR SERVICETransonic Flowprobes are precalibrated at the factory with equipment that has been calibrated traceable to the standards of National Institute of Standards and Technology and to Transonic Systems Inc. equipment performance standards. At purchase, Flowprobes are issued a Certificate of Calibration valid for one year. Probes may be returned to Transonic for recalibration if Calibration Certification is required for GLP studies.

Transonic will also perform repairs on Flowprobes which have been damaged or cut. Contact Transonic or your local distributor for an RMA # (Return Materials Authorization).

Volume Flow

Volume Flow

RL-20-ds-A4 Rev. D 12/12

PS-Series, V-Series, PR-Series, PAU-Series & PMP-Series

Perivascular Flowprobe Specifications

PROBE SIZE & SERIES

VESSEL OD BIDIRECTIONAL FLOW OUTPUTS ACCURACY SPECIFICATIONS5ULTRASOUNDFREQUENCYMA-ACUTE

APPLICATIONMC-CHRONIC APPLICATION

RESOLUTION1LOW FLOW ( SCALE)2

STANDARD SCALE2

MAX FLOW3 (STD SCALE)

ZERO OFFSET4

ABSOLUTE ACCURACY

RELATIVE ACCURACY

mm mm ml/min1V output in ml/min

1V output in ml/min

5V output in ml/min

ml/min% of reading

% MHz

0.5PS 0.3 - 0.5 0.3 - 0.48 0.03 1.5 6 30 0.12 15 2 14.4

0.7PS 0.5 - 0.7 0.4 - 0.7 0.05 2.5 10 50 0.2 15 2 9.6

1.5PS 1.2 - 1.5 1.2 - 1.5 0.075 10 40 200 0.8 15 2 4.8

0.5V 0.25 - 0.5 N/A 0.05 2.5 10 50 0.25 15 3 7.2

0.7V 0.35 - 0.7 N/A 0.075 5.0 20 100 0.5 15 3 4.8

1PR 0.7 - 1.2 0.7 - 1.0 0.05 5 20 100 0.2 10 2 7.2

1.5PR 1.2 - 1.8 1.0 - 1.5 0.075 10 40 200 0.4 10 2 4.8

2PS 1.5 - 2.0 1.3 - 1.8 0.1 25 100 500 1 10 2 3.6

2.5PS 1.8 - 2.5 1.5 - 2.4 0.1 25 100 500 1 10 2 3.6

3PS 2.5 - 3.7 2.4 - 3.4 0.4 50 200 1 L 2 10 2 3.6

4PS 3.3 - 4.4 3.0 - 4.0 0.8 100 400 2 L 4 10 2 2.4

6PS 4.4 - 6.6 4.0 - 6.0 2.0 250 1 L 5 L 10 10 2 1.8

8PS 6.6 - 8.8 5.8 - 8.0 4.0 500 2 L 10 L 20 10 2 1.2

10PS 8.3 - 11.0 7.3 - 10.0 4.0 500 2 L 10 L 20 10 2 1.2

12PS 9.8 - 13.0 8.6 - 12.0 8.0 1 L 4 L 20 L 40 10 2 0.9

14PS 11.3 - 15.0 10 - 14 8.0 1 L 4 L 20 L 40 10 2 0.9

16PS 13.3 - 17.7 12 - 16 20.0 2.5 L 10 L 50 L 100 10 2 0.6

20PS 16.0 - 21.0 14 - 19 40.0 2.5 L 10 L 50 L 100 10 2 0.6

8PAU 6 - 8 6 - 7 4 500 2 L 10 L 20 10 2 3.6

10PAU 8 - 10 8 - 9 4 500 2 L 10 L 20 10 2 3.6

12PAU 9 - 12 9 - 11 8 1 L 4 L 20 L 40 10 2 2.4

14PAU 11 - 14 11 - 13 8 1 L 4 L 20 L 40 10 2 2.4

16PAU 12 - 16 12 - 15 20 2.5 L 10 L 50 L 100 10 2 1.8

20PAU 16 - 20 16 - 19 20 2.5 L 10 L 50 L 100 10 2 1.8

24PAU 19 - 24 19 - 23 40 5 L 20 L 100 L 200 10 2 1.2

28PAU 22 - 28 22 - 27 40 5 L 20 L 100 L 200 10 2 1.2

32PAU 25 - 32 25 - 316 80 10 L 40 L 200 L 400 10 2 0.9

36PAU 28 - 36 28 - 356 80 10 L 40 L 200 L 400 10 2 0.9

2PMP 1.5 - 2.5 N/A 0.1 25 100 500 1 15 2 3.6

3PMP 2.5 - 3.7 N/A 0.4 50 200 1 L 2 15 2 3.6

4PMP 3.3 - 4.4 N/A 0.8 100 400 2 L 4 15 2 2.4

6PMP 4.4 - 6.6 N/A 2.0 250 1 L 5 L 10 15 2 1.8

8PMP 6.6 - 8.8 N/A 4.0 500 2 L 10 L 20 15 2 1.2

10PMP 8.3 - 11.0 N/A 4.0 500 2 L 10 L 20 15 2 1.2

12PMP 9.8 - 13.0 N/A 8.0 1 L 4 L 20 L 40 15 2 0.9

14PMP 11.3 - 15.0 N/A 8.0 1 L 4 L 20 L 40 15 2 0.9

1. Resolution: represents the smallest detectable change in flow, a factor in accuracy.2. Probes operate in either low flow or normal flow scales, determined by the flow range under study. Probes measure bidirectional flow up to 5 times the

selected scale setting. The scale settings calibrates the 1 volt reference signal for data collection; the linear range of the Flowmeter is equal to 5V. By using the low flow button, measurement sensitivity is increased four fold. This linear overrange is important for the proper recording of highly pulsatile peak flows.

3. Maximum Range for each Probe reflects the highest flow rate that can be processed and displayed via the analog connector.

4. Zero offset on individual Probes is often lower than this value.5. The Absolute Accuracy percentage can be raised to relative accuracy levels by in situ calibration.6. 32-36PAU Probes are only supplied with acute liners & may require modification for chronic use.

www.transonic.com

Probe Width

Lumen

Probe Length

Reflector Depth

Physical Specifications

PROBE SIZE & SERIES

PROBE PROBE LINER PROBE SHELL CABLE

WEIGHT ID DEPTH W/ FLANGE DEPTH LENGTH LENGTH DIAMETER

grams mm mm mm mm m mm

8PAU 2.0 8 7 8 16 2.0 2.7

10PAU 2.5 10 7 8 19 2.0 2.7

12PAU 4.0 12 10 10 21 2.0 2.7

14PAU 5.5 14 12 12 24 2.0 2.7

16PAU 9.0 16 13 14 26 2.0 2.7

20PAU 14.0 18 or 20 14 15 31 2.0 2.7

24PAU 22.5 22 or 24 18 18 36 2.0 2.7

28PAU 30.3 26 or 28 19 20 41 2.0 2.7

32PAU 45 30 or 32 22 22 46 2.0 2.7

36PAU 59 34 or 36 23 23 51 2.0 2.7

PROBE SIZE & SERIES

PROBE BODY REFLECTOR HANDLE CABLE

WEIGHT LENGTH WIDTH LENGTH WIDTH DEPTH LENGTH LENGTH DIAMETER

grams mm mm mm mm mm cm m mm

0.5PS 0.09 3.2 2.3 lumen = 0.471 1.02 53 60 cm 1.0

0.7PS 0.12 3.2 2.7 lumen = 0.701 1.22 53 60 cm 1.0

1.5PS 0.23 4.25 3.75 lumen = 1.651 2.02 N/A 60 cm 1.25

0.5V 0.2 6.5 4.0 2.0 1.5 1.1 53 60 cm 1.5

0.7V 0.25 7.6 3.5 2.5 1.7 1.8 53 60 cm 1.5

1PR 0.2 6.5 4.0 2.0 1.5 1.1 N/A 60 cm 1.5

1.5PR 0.25 7.6 3.5 2.5 1.7 1.8 N/A 60 cm 1.5

2PS 0.3 8.7 3.3 3.3 2.0 2.5 N/A 1.0 1.5

2.5PS 0.3 8.7 3.3 3.3 3.2 2.5 N/A 1.0 1.5

3PS 1.2 9.0 5.0 3.5 4.0 3.7 N/A 1.0 2.0

4PS 1.5 13.3 6.0 3.8 5.5 4.4 N/A 1.0 2.0

6PS 2.7 13.5 6.7 4.0 7.8 6.6 N/A 1.0 2.5

8PS 5.0 18.8 7.5 6.0 8.2 8.8 N/A 1.0 2.5

10PS 5.3 18.8 8.5 6.0 10.0 11.0 N/A 1.0 2.5

12PS 9.3 22.5 8.5 6.2 12.0 13.0 N/A 1.0 3.0

14PS 11.6 26.2 8.5 7.5 14.5 15.0 N/A 1.0 3.0

3.16PS 16.6 36.0 10.0 9.0 17.0 17.7 N/A 1.0 3.0

20PS 20.2 31.0 9.0 9.0 21.0 21.0 N/A 1.0 3.0

2PMP 14 7 5.5 3.0 3.0 2.7 17.5 2.0 2.2

3PMP 17 9 7.4 4.0 4.0 3.5 17.5 2.0 2.2

4PMP 17 10 6.8 3.4 5.0 4.6 17.5 2.0 2.2

6PMP 17 12.7 7.7 4.1 7.8 6.6 17.5 2.0 2.2

8PMP 21 15.2 8.5 4.6 9.0 8.6 17.5 2.0 2.2

10PMP 21 16.5 9.4 5.2 11.0 10.8 17.5 2.0 2.2

12PMP 21 19.5 10 5.8 13.0 12.8 17.5 2.0 2.2

14PMP 26 25 11 7.6 14.5 17.5 17.5 2.0 2.2

Liner Depth

Shell Depth

Shell Length

ID

Probe Length

Probe Width

Reflector Width

Reflector Depth

Reflector Length

1. Lumen is the ultrasonic window within the reflector. Dimensions are approximately equal.

2. Reflector depth is the external dimension for Nanoprobes.

3. Handle comes standard with indicated MA- acute Probes. MC- chronic or custom orders are available without a handle.

For Use With TS410 Flow Modules

Volume Flow

Precision Tubing Flowsensors

Volume Flow

Measurevolumeflowinmostnon-aeratedliquidsincludingsaline,buffersolutions,blood,water&evendieselfuelwithhighresolutionandlowzerooffset.ChooseClamp-onorInlinetomatchyourflowcircuitrequirements.

ME-PXL Clamp-on Flowsensors Non-contactClamp-onSensorsdonotbreakcircuitsterility

Preclinicalandmedicalflowapparatusdesign

SmalldiameterSensorsforindustrialuseInnovativetransit-timetechnologyrevolutionizedbloodflowmeasurementinmedicaltubingapplicationswiththeseFlowsensorsthatclipontotheoutsideofflexibletubingtomeasuretheflowwithin.TheyhavebecomethestandardforOEMmedicaldesignandpreclinicalextracorporealusebyprovidingnon-invasivemeasurementwithhighaccuracyandstability.Measurementsarereliableeveninchallengingelectromagneticenvironments.

Theeasyclip-onoperationofthePXLFlowsensorsalsomaketheseSensorsidealforindustrialflowmeasurementapplicationswhenprocesstestingneedstobequick,repeatableandappliedtomultiplecircuitswithoutflowinterruption.Unlikelargediameterindustrialflowmeasurementdevices,TransonicprovideshighresolutionClamp-onSensorsforsmalldiametertubingsdownto1/8OD.

ME-PXN Inline FlowsensorsMeasureflowoverawidedynamicrange

Flexibilityfortubingcircuitsthatmayvaryorarestillinthedesignphase

HighestsensitivitySensorsforlowflowapplicationsPXNInlineFlowsensorsutilizeaschemeofultrasonicilluminationthatmakesitpossibletomanufactureaflow-throughSensorwithasmooth,cylindricalinteriorwithoutcompromisingmeasurementaccuracy.TheseFlowsensorsoffermoreflexibilityoverClamp-onSensorsasmeasurementcalibrationdoesntdependonthetypeandexactsizeoftubingonwhichtheyareused.SmalldiameterPXNInlinesareidealforlowflowisolatedheartorperfusedorganstudies.

RL-28-fly Rev. C 1/13


SENSOR SIZE

PHYSICAL SPECIFICATIONS1

DIMENSION ALONG TUBE

DEPTH LENGTH

in mm in mm in mm

2PXL 0.8 21 0.7 17 1.3 32

3PXL 0.8 21 0.7 17 1.3 32

4PXL 0.9 23 0.8 20 1.4 35

5PXL 0.9 23 0.8 20 1.4 35

6PXL 1.0 24 0.9 23 1.5 39

7PXL 1.0 26 1.0 25 1.7 42

8PXL 1.1 28 1.0 24 1.7 44

9PXL 1.3 33 1.0 25 1.8 47

10PXL 1.2 32 1.1 27 2.0 51

11PXL 1.4 35 1.1 28 2.2 56

12PXL 1.5 38 1.2 31 2.4 61

14PXL 1.6 41 1.4 36 2.6 66

16PXL 1.9 47 1.5 39 3.0 75

20PXL 2.3 58 1.8 46 3.7 93

ME-PXL Series Clamp-on Flowsensors

APPLICATIONS Artificial

Heart&VADPerformance

MedicalDevice&PumpEngineering

Manufacturing&ComplianceFlowTesting

TransonicPXLClamp-onTubingFlowsensorsclipontheoutsideofflexiblelaboratorytubing.Nophysicalcontactismadewiththefluidmedia.AthinsmearofVaselineorpetroleumjellyshouldbeappliedtothesectionoftubingwheretheSensorisappliedtoprovideagoodsealbetweenthetransducersandthetubeforbestultrasonicsignaltransmission.PXLSeriesFlowsensorscanbefactorycalibratedandprogrammedforupto4differentfluid,temperature,tubing,andflowratecombinations.Sensorsizeisdeterminedbytheoutsidediameterofthetubing.StandardPXLSensorsaresizedin1/16increments.Metricsizesareavailableformetrictubing.

Easy clip on application of PXL Sensor to tubing

1. Standard cable length is 2 meters.

SENSOR SIZE

BIDIRECTIONAL FLOW OUTPUTS SYSTEM ACCURACY SPECIFICATIONS2

ULTRASOUNDFREQUENCYRESOLUTION1

LOW FLOW ( SCALE)

STANDARD FLOW SCALE

MAX FLOW (STD SCALE)

MAX ZERO OFFSET

ABSOLUTE ACCURACY

LINEARITY

ml/min1V output in ml/min

1V output in ml/min

5V output in L/min

ml/min% of reading

% MHz

2PXL 0.5 50 200 1 4.0 10 4 3.6

3PXL 1.0 100 400 2 8.0 10 4 3.6

4PXL 1.0 100 400 2 8.0 10 4 2.4

5PXL 1.0 100 400 2 8.0 10 4 2.4

6PXL 2.5 250 1 L 5 15 10 4 2.4

7PXL 5 500 2 L 10 30 10 4 1.8

8PXL 5 500 2 L 10 30 10 4 1.8

9PXL 5 500 2 L 10 30 10 4 1.8

10PXL 10 1 L 4 L 20 60 10 4 1.2

11PXL 10 1 L 4 L 20 60 10 4 1.2

12PXL 10 1 L 4 L 20 60 10 4 1.2

14PXL 25 2.5 L 10 L 50 150 10 4 1.2

16PXL 25 2.5 L 10 L 50 150 10 4 1.2

20PXL 50 5 L 20 L 100 300 10 4 0.9Calibration is dependent on tubing material, wall thickness, ultrasound velocity of liquid flowing through the tube & temperature.1. Resolution represents the smallest detectable flow change at 0.1 Hz filter (average flow output).2. Stated system accuracy specifications apply to PXL Flowsensors with TS410 Flow Modules. (a) Absolute accuracy is comprised

of zero stability, resolution and linearity effects. Stated values apply when flow rate is greater than 5% of maximum range and zero offset is nulled. (b) If the Sensor is calibrated on-site with the system Flow Module for the tubing and liquid in use, absolute accuracy may be improved to the Linearity value. (c) On-site calibration is recommended if the Sensor is routinely used to measure flows less than 5% of the maximum range to account for non-linearities associated with flow profile.

SENSOR SIZE

TUBING SPECIFICATIONS INLINE FLOWSENSOR CALIBRATION RANGES1

TUBING ID BARB OD LOW FLOW (1/4 SCALE)2 STANDARD FLOW (FULL SCALE)

in mm in mmLOWER LINEAR

LIMIT3,4MAX MEASUREMENT

RANGE5LOWER

LINEAR LIMITMAX MEASUREMENT

RANGE5

1PXN 3/64 1.2Flexible tubing

5 ml/min -25 to +25 ml/min 10 ml/min -100 to +100 ml/min

2PXN 1/16 1.6 10 ml/min -50 to +50 ml/min 20 ml/min -200 to +200 ml/min

3PXN 3/32 2.4 25 ml/min -125 to +125 ml/min 50 ml/min -500 to +500 ml/min

4PXN 1/8 3.2 0.16 4.0 50 ml/min -250 to +250 ml/min 100 ml/min -1 to +1 L/min

5PXN 3/16 4.8 0.23 5.8 100 ml/min -500 to +500 ml/min 200 ml/min -2 to +2 L/min

6PXN 1/4 6.4 0.3 7.6 250 ml/min -1.25 to +1.25 L/min 500 ml/min -5 to +5 L/min

10PXN 3/8 9.5 0.44 11.1 500 ml/min -2.5 to +2.5 L/min 1 L/min -10 to +10 L/min

13PXN 1/2 12.7 0.58 14.7 1 L/min -5 to +5 L/min 2 L/min -20 to +20 L/min

16PXN 5/8 15.9 0.72 18.3 2.5 L/min -12.5 to +12.5 L/min 5 L/min -50 to +50 L/min6

19PXN 3/4 19.1 0.86 21.9 2.5 L/min -12.5 to +12.5 L/min 5 L/min -50 to +50 L/min6

25PXN 1 25.4 1.14 29.0 5 L/min -25 to +25 L/min 10 L/min -100 to +100 L/min6

ME-PXN Series Inline Flowsensors

APPLICATIONS IsolatedOrgan

Studies

FlowPhantoms&CirculatoryModels

VolumeFlowSensitiveApplications

1. Stated system accuracy specifications apply to PXN Flowsensors with TS410 Flow Modules

1. To meet accuracy specifications, Flowsensors should be calibrated for the flow rate range of use.2. Flowsensors calibrated for the low flow range should be used with the Flow Module in 1/4 Scale Mode.3. Measurements below the Lower Linear Limit may deviate from the stated accuracy specification.4. Custom calibration is available for average flow rates below the lower limit. This may compromise accuracy

for the max measurement range.5. Range includes zero: any flow peaks exceeding the Max Flow Value (-5 volt to +5 volt range) will be clipped.6. Standard calibration range is up to 30 L/min; contact factory for availability of higher flow rate calibrations.

PXNInlineFlowsensorsspliceintolaboratorytubingandmeasurevolumetricflowofbloodandotherfluids.Theyofferthemostflexibilityforflowmeasurementincircuitswheretubingrequirementshavenotbeenformalized.Thefour-transducerSensordesignofferspreciseandaccurateflowmeasurementforloworhighflowrates,steadystateorpulsatileflows.FlowresolutionisscaledtoSensorsize,andflowismeasuredaccuratelyacrosstheSensorsfulldynamicrangewithlittleeffectfromturbulence.TheSensorssmoothroundflowchanneliseasytocleananddoesnottrapairbubblesthatcandegradeultrasonicperformance.Sizes4PXN-25PXNhavebarbed,rigidtubingendswhichmateeasilywithflexiblelaboratorytubing.Plasticclampsmaybeappliedtotheoutsideforaddedsecurityinhighpressurecircuits.Miniaturesize1PXN-3PXNSensorsarefabricatedaroundflexibletubingwhichmaybecuttolengthforinsertionintosmalltubingcircuitsorperfusionapparati.TheflexibletubingendsmaybeexpandedtofitoverlargertubingOD(seeRL-31-tn).PXNInlineSensorscanbecalibratedandpre-programmedforupto4fluid,temperature,andflowratecombinations.

Size 4PXN - 25PXN have barbed, rigid tubing ends

SENSOR SIZE

BIDIRECTIONAL FLOW OUTPUTS SYSTEM ACCURACY SPECIFICATIONS1 PHYSICAL SPECIFICATIONS4

ULTRASOUNDFREQUENCYRESOLUTION

LOW FLOW

( SCALE)

STANDARD FLOW SCALE

MAXFLOW

(STD SCALE)

MAX ZERO OFFSET2

ABSOLUTE ACCURACY

LINEARITY3TOTAL

LENGTH W/ TUBE ENDS

CASE LENGTH W/O TUBE ENDS

at 10 Hz in ml/min

1V output in ml/min

1V output in ml/min

5V output in L/min

ml/min% of reading

% in mm in mm MHz

1PXN 0.02 5 20 100 0.4 8 2 3.9 100 0.3 8 9.6

2PXN 0.02 10 40 200 0.6 4 2 3.9 100 0.5 12 9.6

3PXN 0.05 25 100 500 1 4 2 3.9 100 0.6 14 7.2

4PXN 0.1 50 200 1 L 2 4 2 1.0 25 0.6 16 4.8

5PXN 0.2 100 400 2 L 4 4 2 1.2 32 0.8 20 3.6

6PXN 0.5 250 1 L 5 L 10 4 2 1.6 41 1.0 26 2.4

10PXN 1 500 2 L 10 L 20 4 2 2.0 51 1.3 33 1.8

13PXN 2 1 L 4 L 20 L 40 4 2 2.7 69 1.8 45 1.2

16PXN 5 2.5 L 10 L 50 L 70 4 2 3.3 83 2.1 52 0.9

19PXN 5 2.5 L 10 L 50 L 100 4 2 4.0 101 2.5 64 0.9

25PXN 10 5 L 20 L 100 L 200 4 2 5.0 128 3.1 80 0.6

2. Zero offset can be eliminated by Zero Adjustment prior to measurement

3. Within specified calibration range.

4. Standard cable length is 1.85 meters.

CALIBRATION CODES

FLUIDTEMPERATURE

23C 37C CUSTOM2

Water/Saline H2023 H2037 H20TX

Blood BL23 BL37 BLTX

Glycerine 40%, Water 60% (vol)1 GL23 GL37 GLTX

Custom Glycerine2 GLX23 GLX37 GLXTX

Krebs Solution KR23 KR37 KRTX

Custom Fluid2 FX23 FX37 FXTX

FLOW RANGE CATALOG CODE

Standard Flow SF

Low Flow LF

Custom Flow2 XF

Catalog #: ME PXN - - - Size Fluid/Temperature + Flow Rate Calibration Codes

Up to four per Flowsensor

How to Order a Flowsensor1. ME-indicatescompatibilitywithTS410FlowModules.

2. DetermineifaPXNInlineorPXLClamp-onFlowsensorisbetterforyourapplication.

a. IfPXNInline,choosetheSensorsizethatwillbestmatchthecircuitIDandanticipatedflowrates.

b. IfPXLClamp-on,choosetheSensorsizethatmatchesyourtubingOD.Thenspecifytubingtype.Supplya60cmlengthsampleifyourtubingisnotonourstocktubinglist.

3. SpecifythefollowinginformationforeitherFlowsensortype:

a. Anticipatedflowrange(averageandpeak)

b. Fluidandtemperatureforcalibration

4. Usethecodetables(right)toconstructthecatalognumber.

5. ContactCustomerServiceforanynon-standardrequests.

Example:ME4PXN-BL37SFKR37SFFX37LF,calibratedfor3differentuses:

Bloodat37Catstandardflowrate

Krebsat37Catstandardflowrate

Customfluidat37Catlowflowrate

FLOW MODULE COMPATIBILITYTS410TubingFlowModule

SENSOR SIZEPXNInlineFlowsensorsaresizedtofittheinternaldiameteroftheflexibletubinginyourcircuit.PXLClamp-onFlowsensorsclamparoundtheouterdiameterofflexibletubing.UsethetubingspecificationtablestodeterminethebestSensorsizeforyourtubingrequirements.

TUBING DIAMETER FOR PXN INLINESThePXNSensorthatmostcloselymatchesthecircuittubingIDshouldbeusedsothatperturbationsinfluiddynamicsareminimized.Reducingthetubingdiametercanaddresistancetoflow.

TUBING REQUIREMENTS FOR PXL CLAMP-ONSTransmittingtheultrasoundsignalthroughthetubingwallrequiresasnug,compressivefit,sotheSensorsizeisdependentonthetubingofthecircuit.Medicalgradeandlaboratorytubings(PVC,silicone,polyurethane)aregenerallycompatibleforusewithPXLSensors.Rigid

PVCandacrylic(Lucite)arenot.WestockcommonPVCtubingtypes.Forothertubingtypesormetricsizes,a60cmsampleisrequiredforSensorcalibration.

EXPECTED FLOW RATESTransonicFlowsensorsizesarescaledtoachievethehighestresolutioninflowmeasurement.ThesmallertheSensor,thehigherthesensitivitytolowvolumeflowsandthelowertheoffsetatzeroflow(seespecificationstable).AllPXLandPXNSensorshavetwodynamicflowranges:standardflowrange(fullscale)andlowflowrange(1/4scale).Sensorsarefactorycalibratedforthehighestaccuracyinthespecifiedflowrange.Tomeetperformancespecifications,indicateloworstandardflowwhenordering.Ifnoflowrangeisindicated,Sensorswillbecalibratedinthestandardflowrange.Calibrationforflowsbelowthelowerlinearlimitmayberequestedandwillincuranextracharge.

Considerations for Choosing the Most Appropriate Flowsensor

1. Glycerine calibration is custom for 1PXN-3PXN2. All custom requirements (X) incur an extra charge

Small 1 - 3PXNs use flexible tubing to connect with the fluid circuit

CLAMP-ON FLOWSENSOR TUBING SPECIFICATIONS

FRACTIONAL (1/16 INCH INCREMENTS) METRIC

SENSOR SIZE

TUBING IDWALL

THICKNESSTUBING

ODSTOCK

TUBING2TUBING OD SENSOR #

WITH SUFFIXmm

2PXL 3/32 1/321 1/8-5/32 A, B 4 2PXL-M4

3PXL 1/8 1/321 3/16-7/32 A 5 3PXL-M5

4PXL 1/8 1/16 1/4 A, B 67

4PXL-M64PXL-M7

5PXL 3/16 1/16 5/16 A 8 5PXL-M8

6PXL 1/4 1/16 3/8 A, B, C 9 6PXL-M9

7PXL 1/4 3/32 7/16 A, B, C 10 7PXL-M10

8PXL 3/8 5/16

1/163/32

1/2 A, BN/A 12 8PXL-M12

9PXL 3/8 3/32 9/16 A, B, C 14 9PXL-M14

10PXL 1/2 1/16 5/8 A, B 16 10PXL-M16

11PXL 1/2 3/32 11/16 A, B

12PXL 1/2 1/8 3/4 A, B 20 12PXL-M20

14PXL 5/8 11/16

1/83/32

7/8 AN/A 23 14PXL-M23

16PXL 3/4 1/8 1 A 25 16PXL-M25

20PXL 1 1/8 11/4 A

SENSOR SIZE

CLAMP-ON FLOWSENSOR CALIBRATION RANGES1

LOW FLOW ( SCALE)2 STANDARD FLOW (FULL SCALE)

LOWER LINEAR LIMIT3,4

MAX MEASUREMENT RANGE5

LOWER LINEAR LIMIT

MAX MEASUREMENT RANGE5

2PXL 50 ml/min -250 to +250 ml/min 100 ml/min -1 to +1 L/min

3PXL, 4PXL, 5PXL 100 ml/min -500 to +500 ml/min 200 ml/min -2 to +2 L/min

6PXL 250 ml/min -1.25 to +1.25 L/min 500 ml/min -5 to +5 L/min

7PXL, 8PXL, 9PXL 500 ml/min -2.5 to +2.5 L/min 1 L/min -10 to +10 L/min

10PXL, 11PXL, 12PXL 1 L/min -5 to +5 L/min 2 L/min -20 to +20 L/min

14PXL, 16PXL 2.5 L/min -12.5 to +12.5 L/min 5 L/min -50 to +50 L/min6

20PXL 5 L/min -25 to +25 L/min 10 L/min -100 to +100 L/min6

Suffix ifMetric OD

Catalog #: ME PXL - - - - Size Fluid/Temperature + Flow Rate Calibration Codes

Up to four per Flowsensor

Tubing: Example:ME6PXLBL37SFH2023LFisfor3/8ODtubing,calibratedfor2uses:

Bloodat37CatStandardFlowRatesonTygonS-50-HL(1/4IDx1/16wall)

Waterat23CatLowFlowRatesonTygonR-3603(1/4IDx1/16wall)

Considerations for Choosing the Most Appropriate Flowsensor

1. To meet accuracy specifications, Flowsensors should be calibrated for the flow rate range of use.2. Flowsensors calibrated for the low flow range should be used with the Flow Module in 1/4 Scale Mode.3. Measurements below the Lower Linear Limit may deviate from the stated accuracy specification.4. Custom calibration is available for average flow rates below the lower limit. This may compromise accuracy

for the maximum measurement range.5. Range includes zero: any flow peaks exceeding the Max Flow Value (-5 volt to +5 volt range) will be clipped.6. Standard calibration range is up to 30 L/min; contact factory for availability of higher flow rate calibrations.

1. In sizes 2-3PXL ratio of tubing wall thickness to OD must not exceed 1:5 for PVC; 1:3 for silicone

2. A= Tygon R-3603, B= Tygon S-50-HL, C= MEDIFLEX

STANDARD CONNECTOR TYPES CP16:Plastic16-pin;1-6PXN

CC16:Metal16-pin;10-25PXN&allPXLs

TS410 EXTENSION CABLESPXNscomewithastandardcablelengthof1.85meter.PXLscomewitha2metercable.ExtensioncablesareavailableforallSensorsizesexcept1-2PXN

CC16-S-CC16:Standard1meter

CC16-X-CC16:Specifiedcustomlength;upto5meterstandard

MRIsafe:specifyCP16connector

Specify type and size (ID x wall x OD)

FLUID / TEMPERATURE CALIBRATIONSincefluiddensityandtemperaturealterthetransittimeoftheultrasoundsignalandaffecttheacousticpropertiesofthetubing,wecalibratetheSensorspecificallyfortheseparameters.Operationalfluidtemperatureshouldbewithin2Cofthespecifiedcalibrationtemperature.Specifyupto4tubing,fluid,temperature,&flowratecombinationsforcalibration.FluidsamplesandMSDSinformationmayberequiredforcustomcalibrationrequests.

CALIBRATION CERTIFICATESSensorsaresuppliedwithacertificateofcalibrationforspecifieduse.Yearlyrecertificationisrecommended.

ME-PXL Clamp-on Flowsensor Parameters





www.transonic.com


Volume FlowVolume FlowVolume Flow

CLEANING & STERILIZATIONTransonicTubingFlowsensorsmaybecleanedwithasolutionofmildsoapandwarmwater(

Volume Flow

Precision Nanoprobes for MicePS-Series, PR-Series & V-Series Flowprobesfor use with TS420 Flowmeter Module

SizedtoFitMouseAnatomy

UnprecedentedCapabilityContinuousreal-timevolumeflowmeasurementsforcalculatingcardiacoutput,strokevolumeandvascularresistance.

Applicationsinclude:renalbloodflow,autoregulation,carotidthrombosisocclusion&femoralhindlimbstudies.

Foracuteorchronicapplicationswitharangeofcustomizablefeatures.

GoldStandardAccuracyUltrasonicTransit-timeTechnology

Waveformsdemonstratesignaturezerobaselinestabilityandhighresolutionevenwithflowslessthan1mL/min.

MURINE BLOOD FLOW MAPPING

VESSEL SUGGESTED PROBE

Ascending Aorta 1.5PSL

Lower Thoracic Aorta 1PR

Pulmonary Artery 1PR

Carotid Artery 0.5PS, 0.5V

Femoral Artery 0.5PS

Mesenteric Artery 0.7PS

Renal Artery 0.5PSL

Portal Vein 1PR

RL-18-fly-A4 Rev. D 11/12

Ascending AortaFor Cardiac Output

For Peripheral Vessels

VESSEL OD BIDIRECTIONAL FLOW OUTPUTS ACCURACY SPECIFICATIONS ULTRASOUND

PROBE SIZE & SERIES

MA-ACUTE APPLICATION

MC-CHRONIC APPLICATION

RESOLUTIONLOW FLOW ( SCALE)

STANDARD FLOW

MAX FLOW (STD FLOW)

ZERO OFFSET

ABSOLUTE ACCURACY

RELATIVE ACCURACY

FREQUENCY

mm mm ml/min ml/min ml/min ml/min ml/min % % MHz

0.5PS 0.3 - 0.5 0.3 - 0.48 0.03 1.5 6 30 0.12 15 2 14.4

0.7PS 0.5 - 0.7 0.4 - 0.7 0.05 2.5 10 50 0.2 15 2 9.6

1.5PS 1.2 - 1.5 1.2 - 1.5 0.075 10 40 200 0.8 15 2 4.8

1PR 0.7 - 1.2 0.7 - 1.0 0.05 5 20 100 0.2 10 2 7.2

0.5V 0.25 - 0.5 Acute use only

0.05 2.5 10 50 0.25 15 3 7.2

0.7V 0.35 - 0.7 0.075 5.0 20 100 0.5 15 3 4.8

Specifications & Accessories for Mouse Applications

PROBE SIZE & SERIES

CABLE ORIENTATION

PROBE BODY CABLE

WEIGHT LENGTH WIDTH HEIGHT LUMEN LENGTH DIAMETER

0.5PS any (B, S or L) 0.09 g 3.2 mm 2.3 mm 1.0 mm 0.47 mm 60 cm 1.0 mm

0.7PS back only 0.12 g 3.2 mm 2.7 mm 1.2 mm 0.70 mm 60 cm 1.0 mm

1.5PS lateral only 0.23 g 4.25 mm 3.75 mm 2.0 mm 1.65 mm 60 cm 1.25 mm

1PR back or side 0.2 g 6.5 mm 4.0 mm 1.1 mm 1.5 mm 60 cm 1.5 mm

0.5V back only 0.2 g 6.5 mm 4.0 mm 1.1 mm 1.5 mm 60 cm 1.5 mm

0.7V back only 0.25 g 7.6 mm 3.5 mm 1.8 mm 1.7 mm 60 cm 1.5 mm

0.5PSL Probe on Mouse Renal Artery with Flowtrace

www.transonic.com

See Tools & Techniques for Hemodynamic Studies in Rodents (RL-5-wb) for in depth application information and Perivascular Flowprobe Flyer (RL-26-fly) for more information on ordering.

WidthLumen

Length

Height

Nanoprobes (PS-Series) are very much smaller than V-Series Flowprobes.

ORDERING PRECISION PROBES MA-prefix:standardacuteconfiguration;specifysizeandcableorientation.Acute0.5PS&0.7PSandV-SeriesProbessuppliedwith5cmhandle.

MC-prefix:customorchronicconfiguration;specifysize,cableorientation&length,connectortype,&calibrationoption.

STABILIZATION ACCESSORIESForprecisionmeasurements,NanoprobesandV-Seriesshouldbestabilizedwithamicromanipulatorduringacuteuse.

CuffsforCA4:SiliconeFlange(AAPC103);RigidCuffwithsetscrew(AAPC105)

Surgicalmesilenemeshforimplantstabilization

CONNECTORSStandardacuteuseCRA10Redel

Chronicmicro4-pinconnectorscomewithacalibrationkey:CA4B(back)&CA4S(side)

EXTENSION CABLESCRA10-S-CRA10:10-pinacute;1.25meterstandard

CA4-S-CRA10:4-pinchronic;1.8meterstandard

Note: Nanoprobes must be used with the proper length extension cable (probe + cable = approx. 185 cm) to meet performance specifications.

Chronic MC1.5PSL with CA4S Connector for Mouse Ascending Aorta

MA0.7PSB

MA0.5PSB

MC0.5PSL

MC0.5PSS

MC0.7VB

MA0.5VB

Volume Flow

Precision Flowprobes for Rats & RodentsPS-Series & PR-Series for use with TS420 Flowmeter Module

Validated,Referenced,TrustedCitedinover500studiesinrats&rodents

GoldStandardaccuracy

UltrasonicTransit-timeTechnology

BloodFlowApplicationsContinuousreal-timevolumeflowmeasurementsfornumerousapplicationsincluding:cardiacoutput,renalorportalhypertension,hemorrhagicshock,ischemia/reperfusion,carotidthrombosisandstrokemodels.

Functional&FlexibleCompatiblewithelectricalswivelsandtethers

Widerangeofcustomizablefeatures

Acuteandchronicuseinvesselsfrom0.25mmdiameterandlarger

RAT BLOOD FLOW (wgt. 250-350 g)

VESSEL SUGGESTED PROBE

Ascending Aorta 2.5PS, 3PS

Abdominal Aorta 2PS

Pulmonary Artery 2PS

Carotid Artery 1PR, 0.7PS

Femoral Artery 1PR, 0.7PS

Mesenteric Artery 1PR

Renal Artery 1PR, 0.7PS

Portal Vein 1.5PR, 2PS

Hepatic Artery 0.5PS

Vena Cava 2PS, 2.5PS

RL-19-fly-A4 Rev. C 12/12

Ascen

dingAorta

PROBE SIZE & SERIES

VESSEL OD BIDIRECTIONAL FLOW OUTPUTS ACCURACY SPECIFICATIONS ULTRASOUND

MA-ACUTE APPLICATION

MC-CHRONIC APPLICATION

RESOLUTIONLOW FLOW ( SCALE)

STANDARD FLOW

MAX FLOW (STD FLOW)

ZERO OFFSET

ABSOLUTE ACCURACY

RELATIVE ACCURACY

FREQUENCY

mm mm ml/min ml/min ml/min ml/min ml/min % % MHz

0.5PS 0.3 - 0.5 0.3 - 0.48 0.03 1.5 6 30 0.12 15 2 14.4

0.7PS 0.5 - 0.7 0.4 - 0.7 0.05 2.5 10 50 0.2 15 2 9.6

1.5PS 1.2 - 1.5 1.2 - 1.5 0.075 10 40 200 0.8 15 2 4.8

1PR 0.7 - 1.2 0.7 - 1.0 0.05 5 20 100 0.2 10 2 7.2

1.5PR 1.2 - 1.8 1.0 - 1.5 0.075 10 40 200 0.4 10 2 4.8

2PS 1.5 - 2.0 1.3 - 1.8 0.1 25 100 500 1 10 2 3.6

2.5PS 1.8 - 2.5 1.5 - 2.4 0.1 25 100 500 1 10 2 3.6

3PS 2.5 - 3.7 2.4 - 3.4 0.4 50 200 1 L 2 10 2 3.6

Specifications & Accessories for Rat ApplicationsORDERING PRECISION PROBES MA-prefix:standardacuteconfiguration;specifysizeandcableorientation.Acute0.5PS&0.7PSProbesaresuppliedwitha5cmhandle.

MC-prefix:customorchronicconfiguration;specifysize,cableorientation&length,connectortype,&calibrationoption.

Probesize:approximatevesseldiameterinmm.

ProbeCableOrientation:

Back(B):perpendiculartovessel.

Side(S):paralleltovessel.

Lateral(L):forascendingaortaviathoracotomy

STABILIZATION ACCESSORIESForprecisionmeasurements,Nanoprobesshouldbestabilizedwithamicromanipulatorduringacuteuse.

CuffsforCA4:SiliconeFlange(AAPC103);RigidCuffwithsetscrew(AAPC105)

CuffsforCM4:SiliconeFlange(AAPC102);RigidCuffwithsetscrew(AAPC104)

Surgicalmesilenemeshforimplantstabilization

CONNECTORSStandardacuteuse:CRA10Redel

Chronicmini4-pinconnectorscomewithserializedcalibrationkeys.

CA4B(back)&CA4S(side)

CM4B(back)&CM4S(side)

EXTENSION CABLESCRA10-S-CRA10:10-pinacute;1.25meterstandardlength

CM4-S-CRA10:4-pinwithspring;1.8meterstandardlength

CA4-S-CRA10:4-pinnarrowgauge;1.8meterstandard

Note: Nanoprobes must be used with the proper length extension cable (probe + cable = approx. 185 cm) to meet performance specifications.

www.transonic.com

Chronic MC2.5PSL with CM4S connector

3PSS

Rigid Delrin Cuff with CA4S connector for chronic Probes

See Tools & Techniques for Hemodynamic Studies in Rodents (RL-5-wb) for in depth application information and Perivascular Flowprobe Flyer (RL-26-fly) for more information on ordering.

2PSB

2.5PSB

1.5PRB

1PRS

Probes pictured larger than actual size.

Volume Flow

RL-34-fly-A4 Rev. B 1/13

A completely new concept in ultrasonic Flowprobe design

Reusabletransducershellwitheasy-to-useflexibleUltrafitLiners

Gold standard measurement using X-pattern transit-time ultrasound illumination

Immediate,accuratebeat-to-beatflowmeasurementswithaminimumofcouplinggel

High resolution quality and uninterrupted signal continuity during intraoperative use & post implant recovery

Incrementallysizedlinersforoptimalvesselfit

Vesselprotectionandcushioningforthedurationoftheimplant

Compatible with TS420 Flowmeters and PhysioGear pocket telemetry system

For Ascending Aorta, Pulmonary Artery, Cardiac Output & other vessel sites

CustomcalibrationavailableforgraftsandVADs

COnfidence Flowprobes

PAU-Series Flowprobes and Ultrafit LinersFor TS420 Flowmeters

Transducer Shell

Chronic Liner Acute Liner

PROBE SIZE & SERIES

ULTRAFIT LINERS

VESSEL OD BIDIRECTIONAL FLOW OUTPUTS ACCURACY SPECIFICATIONS4ULTRASOUND FREQUENCYMA-

ACUTEMC-CHRONIC

RESOLUTION1LOW FLOW ( SCALE)2

STANDARD SCALE2

MAX FLOW (STD SCALE)

ZERO OFFSET3

ABSOLUTE RELATIVE

mm mm mm ml/min ml/min ml/min ml/min ml/min % % MHz

8PAU 8 6 - 8 6 - 7 4 500 2 L 10 L 20 10 2 3.6

10PAU 10 8 - 10 8 - 9 4 500 2 L 10 L 20 10 2 3.6

12PAU 12 9 - 12 9 - 11 8 1 L 4 L 20 L 40 10 2 2.4

14PAU 14 11 - 14 11 - 13 8 1 L 4 L 20 L 40 10 2 2.4

16PAU 16 12 - 16 12 - 15 20 2.5 L 10 L 50 L 100 10 2 1.8

20PAU 18, 20 16 - 20 16 - 19 20 2.5 L 10 L 50 L 100 10 2 1.8

24PAU 22, 24 19 - 24 19 - 23 40 5 L 20 L 100 L 200 10 2 1.2

28PAU 26, 28 22 - 28 22 - 27 40 5 L 20 L 100 L 200 10 2 1.2

32PAU 30, 32 25 - 32 25 - 315 80 10 L 40 L 200 L 400 10 2 0.9

36PAU 34, 36 28 - 36 28 - 355 80 10 L 40 L 200 L 400 10 2 0.9

1. Resolution: represents the smallest detectable change in flow, a factor in accuracy.2. Flowprobes operate in one of two scales: low flow or normal flow, determined by the range of flow under study. Flowprobes measure bidirectional flow up

to 5 times the selected scale setting. The scale settings calibrates the 1 volt reference signal for data collection; the linear range of the Flowmeter is equal to 5 volts. By using the low flow button, measurement sensitivity is increased by a factor of four. This linear overage is important for the proper recording of highly pulsatile peak flows.

3. Zero offset on individual probes is often lower than this value.4. The Absolute Accuracy percentage can be raised to relative accuracy levels by in situ calibration.5. 32PAU and 36PAU Probes are only supplied with acute liners and may require modification for chronic use.

COnfidence Flowprobe Ordering & Specifications

www.transonic.com

ULTRAFIT LINERSUltrafitLinersarerecommendedforsingleuseonly.

COnfidenceFlowprobes (size 8 - 28 mm) are sold with one acute (open) and one chronic (closed) UltrafitLinerofeachsizeavailablepershell.Sizes32&36mmaresuppliedwithacutelinersonly(maybemodifiedforchronicuse).

Replacementlinerssuppliedinpacketsoffiveofeachsizeandstyle.

Custom Catalog #: MC - PAU - - - GAC - Size

Example:

MC-16PAU-WC60-CRS10-GAC(Custom16mmPAU-SeriesProbewith60cmcable,CRS10connectorandacute/chroniccalibration)

Cable Length

Connector PhysioGear

Acute Catalog #: MA - PAUSize

Custom Flowprobe (MC-20PAU-WC200-CS12-GAC) with cable and connector.

Acute (open) liner

Chronic (closed) liner

Acuteconfigurationhasa2metercable & CRA10 Connector

Acute Liners Re-order #: PAUL - A

Chronic Liners Re-order #: PAUL - C Shell Size

Shell Size

Liner Size

Liner Size

Probes come with one of each available liner type & size.


CardiovascularPhysiology

HeartFailureModeling

VentricularFunction&CardiacAssist

ExercisePhysiology

EfficacyofNewCardiacSurgeryTechniques

UltrasoundTransit-timeTechnology

Non-constrictiveFlowprobes

ProbeSizesforMicetoCalves

A c c u r a t e R e p e a t a b l e V a l i d a t e d

Instantaneousflowwaveformsareusedforcalculatingaveragecardiacoutput,strokevolume,peakflow,aorticinputimpedance,systemicvascularresistanceandheartrate.Flowmeasurementswithpressuredataareusedindeterminationofpressurevolumerelationsincardiacfunctionforlargeandsmallanimalmodels.

Volume Flow

Real-time Cardiac Output

Ascending Aorta & Pulmonary Artery Direct Cardiac Output Measurements for Acute & Chronic Applications

Ascending Aorta

Thein vivomeasurementstandardforcontinuouscardiacoutputagainstwhichallothertechnologiesarecompared.Visitwww.transonic.com forapplicationspecificreferences.

PS-Series Probe for small animal models

www.transonic.com

Acute PAU-Series Probe for large animal models

ASCENDING AORTA / PULMONARY ARTERY FLOWPROBE RECOMMENDATIONS

APPLICATION WEIGHT PROBE SIZE & STYLE* CABLE LENGTH CONNECTOR CALIBRATION CUSTOMIZATION

Pig: Acute 25-35 kg MA-16PAU, MA-20PAU WC200 (2 m) CRA10

PAU-Series

GAC- acute/chronic

PAU-Series

Momentary Acute Use: acute open liners

Extended Acute Use: closed liners

Chronic Use: chronic liners

Pig: Chronic25-50 kg MA-16PAU, MA-20PAU

WC50 (50 cm) CRS10, CS12, CB1260-80 kg MA-24PAU, MA-28PAU

Dog: Acute

8-12 kg MA-14PAU, MA-16PAU

WC200 (2 m) CRA10





Dog: Chronic MC-nPAU* WC50 (50 cm) CS12, CRS10

Sheep: Acute35-45 kg MA-16PAU

WC200 (2 m) CRA1045-60 kg MA-20-PAU

Sheep: Chronic35-50 kg MC-20PAU WC40 (40 cm) CRS10, CS12,

CB1250-60 kg MC-24PAU WC50 (50 cm)

Lamb: Acute6-8 kg MA-8PAU, MA-10PAU

WC200 (2 m) CRA1012-15 kg MA-12PAU, MA-14PAU

Lamb: Chronic MC-nPAU* WC100 (1 m) CS12, CRS10

Monkey: Chronic 4 kg

MC-6PSS, MC-8PSS, MC-8PAU, MC-10PAU WC38 (38 cm) CS12, CB12 GAC-

U reflector with silicone for PS-

Rabbit: Acute 3-4.5 kg MA-4PSB WC100 (1 m) CRA10 GA- acute

Rabbit Chronic MC-6PSB WC30 (30 cm) CM4B, CM4S GC- chronic

Rat: Acute

< 270 g MA-2PSB

WC100 (1 m) CRA10 GA- acute Choose cable orientation

270-400 g MA-2.5PSB, MA-2.5PSL

> 400 g MA-3PSB, MA-3PSL

Rat: Chronic MC-nPS* WC10 (10 cm) CM4S, CA4S GC- chronic

Mouse: Acute 17-50 g MA-1.5PSL WC60 (60 cm) CRA10 GA- acute

Mouse: Chronic 17-50 g MC-1.5PSL WC04 (4 cm) CA4S GC- chronic

DOG, PIG, SHEEP & CALF MODELS USE PAU-SERIESCOnfidenceFlowprobes(PAU)obtainahighlevelofaccuracyusingX-pattern,fullflowilluminationwhichallowsuniformsensingofturbulentflow.Ultrafitlinerscushionthevesselandminimizeneedforacousticgel.

Application Recommendations for Large & Small Animals

*n = size based on vessel diameter

All recommendations are based on vessel diameter estimates. Probe choice will depend on surgical approach, species and weight. MA-Probes are configured for standard acute application. Order MC-Probes for any customization or chronic configuration options.

CHRONIC IMPLANTChooseloosefittingProbessothatvesselfills75%-85%oftheProbe

PS-Series:Choosecableconfiguration(side,lateralorback)forpreferredsurgicalapproach

Chooseconnector&cablelength

Selectvesselprotectionmethod:

PAU-Series:UltrafitlinerspadthevesselfromtheProbeedges

PS-Series:siliconeshield(4&6mm)&siliconepaintforProbereflectoredge(2,2.5&3mm)

Allow3-5daysforfibrotictissuegrowthtoprovidegoodcoupling

ACUTE OPEN-CHESTChooseclosefittingProbesforeaseinmaintainingacousticcoupling

UseTransonicapprovedcouplinggelforsignaltransmission

AvoidplacingPS-SeriesProbesdirectlyonthecurvatureoftheaorta

RABBIT, RAT & MOUSE MODELS USE PS-SERIESLittleisolationbetweentheascendingaortaandPAisrequiredtoencirclethevesselwiththeProbesreflector.TheProbetransducerhousingfitseasilyinthethoraciccavity.


Acute&chronicmyocardialhibernation&ischemiamodels

Experimentalrevascularization

CABGpatency&techniques

Stunnedmyocardium

Goldstandardflowmeasurementfornewmyocardialperfusionassessmenttechnologies

Non-constrictiveFlowprobes

DirectvolumeflowinmL/min

A c c u r a t e R e p e a t a b l e V a l i d a t e d

Chronic Dog LCx

Volume Flow

Coronary Artery Blood Flow Measurements

Continuous High Resolution Volume Flow Measurements for Acute & Chronic Applications Using PS-Series & PR-Series Flowprobes

Transonicisthein vivomeasurementstandardforcoronaryarterybloodflowmeasurementsagainstwhichallothertechnologiesarecompared.CoronarybloodflowresearchwithTransonicFlowprobeslaidthefoundationforclinicalsurgicalproceduresandpharmaceuticaltherapiesdevelopedforthesuccessfultreatmentofcoronaryarterydisease.Visitwww.transonic.com tosearchourextensivelistofapplicationspecificreferences.

Pig Coronary Artery

Side View: Probe stabilized with silicone flange

www.transonic.com

FLOWPROBE RECOMMENDATIONS

APPLICATION WEIGHT (KG) LAD LCx SF-SILICONE FLANGE CABLE LENGTH CONNECTOR CALIBRATION

Pig: Acute 25 - 35 MA-2.5PSB MA-3PSB optional WC100 (1 m) CRA10 GA- acute

Pig: Chronic 25 - 35 MC-2.5PSSMC-3PSS MC-3PSS suggested WC50 (50 cm)CM4B, CRS10, CS12 GC- chronic

Piglet: Acute 1.8 - 4 MA-1PRB optional WC60 (60 cm) CRA10 GA- acute

Dog: Acute10 - 15 MA-1.5PRB

optional WC100 (1 m) CRA10 GA- acute18 - 25 MA-2PSB MA-2.5PSB

Dog: Chronic

8 - 12 MC-1.5PRB

suggested WC40 (40 cm) CM4B, CS12, CB12 GC- chronic16 - 25 MC-2PSS MC-2.5PSS

20+ MC-2.5PSS MC-3PSS

Sheep: Acute35 - 45 MA-3PSB

optional WC100 (1 m) CRA10 GA- acute50 - 60 MA-4PSB

Sheep: Chronic35 - 45 MC-3PSS

suggested WC60 (60 cm) CRS10, CS12 GC- chronic50 - 60 MC-4PSS

Baboon: Chronic 10 - 15 MC-2.5PSS suggested WC75 (75 cm) CM4B, CS12 GC- chronic

LowzerooffsetandstablebaselinemakeTransonicthetechnologyofchoiceforcoronaryocclusionmodels.Highresolutionwaveformsareunaffectedbyelectromagneticnoise.Non-magneticMRIsafeFlowprobescanbeordered.TransonicsurgicalFlowprobesarenowusedwidelyduringCABGsurgery,bothoff(beatingheart)andonCPB.

ACUTE OPEN-CHESTChooseaclosefittingProbeforeaseinmaintainingacousticcoupling

UseTransonicapprovedcouplinggelforsignaltransmission(Surgilube;NALCOsuperabsorbant)

StabilizeProbeonbeatingheartwithsiliconecoronaryflange(alsoholdsgelinplace)

CHRONIC IMPLANTChoosealoosefittingProbe,sothatvesselfills75%-85%ofProbelumen

ChoosesidecableconfigurationforseatingProbeinpositiononvessel;providestrainreliefloopincable

Specifysiliconecoronaryflangeforstabilizationonmyocardium

Choosepreferredchronicstyleconnector&cablelengthforprotocol

Tissueingrowthprovidesexcellentsignalcouplingwithin3-5days

See surgical protocol RL-1-sp for more details.

Application Recommendations for LAD, LCx Coronary Artery

1PRS 2PSS2.5PSS

2.5PSB1.5PRB 4PSS3PSB

All recommendations are based on vessel diameter estimates. Probe choice will depend on surgical approach, species and weight. MA-probes are configured for standard acute application. Order MC-probes for any customization or chronic configuration options. See Perivascular Flowprobe Flyer (RL-26-fly) for complete ordering information.

Coronary Flowprobes

Probe with SF- Silicone Flange

Pressure

Pressure Measurement System for Basic Science Research

Pressure

ForoperationofallScisensePressureCatheters(singleanddualpressure)

Simpleelectroniccalibration

Compatiblewithvirtuallyanydataacquisitionsystemandsoftwarepackage

Scisense SP200

SP-1-fly-A4 Rev. A 2/13

SP200 Design & Technology

OPTIMIZED FOR YOUR RESEARCHTheSP200PressureSystemisdesignedtoprovidestable,accurate,high-frequencyresponsepressuremeasurementswhenusedwithScisensesolid-statePressureCatheters.

Withtwo(2)pressurechannels,pushbuttoncontrols,pre-amplifiedoutputsforintegrationwithanydataacquisitionsystem,andoptimizeddampeningcoefficient,theSP200isyourdependableandflexiblesolutionforpressuredetectioninvirtuallyanyresearchapplication.

THE SCISENSE PRESSURE CATHETER DIFFERENCE

Nofluids,flushing,air-bubblesorinterference:

Fluid-filledcathetersrequireconstantmaintenanceandmonitoringtoensuretherearenoleaks,bubbles,orblockagesthatmaycausemeasurementartifacts.Scisensesolid-statePressureCathetersarequicktocalibrateandwilldetectpressureaccurately,freeofartifacts.

Optimalfrequencyresponse:

Higherfrequencypressurewavesmeasuredbyinadequatedeviceswillshowattenuationortaper-off.OurPressureCathetersshowaflatfrequencyresponsewellbeyondtherequirementsofrodentheartrates(600BPM,10Hz).

AccuratedP/dtmeasurement:

MaximumdP/dtisaclassicindicyofcardiaccontractility.Tobeconfidentinthiscalculation,researchersmustbesurethatsignalsarefreeofartifactandattenuation.UsingaScisensePressureCatheterassuresaccuratesignaldetectionandcalculationofdP/dt.

COMMON APPLICATIONS & PARAMETERS

Scisense Pressure Catheter compared to a reference trace and a fluid-filled pressure catheter at 300 BPM (5 Hz). The fluid-filled catheter shows large distortion and wave artifact compared to the Scisense trace which reflects the true pressure waveform of the reference sensor.

HeartRate

Max&MindP/dt

DiastolicPressure

SystolicPressure

DevelopedPressure

ContractilityIndex

RelaxationTime

Tau

MAP

SystemicBloodPressure

VenousBloodPressure

Wedge&PAPressure

AtrialPressure

VentricularPressure

Pulse-WaveVelocity

InterstitialPressures

GastricPressures

Urogenital

Features & Benefits

Simplecontrols

Push-buttoncalibrationandbalancecontrolsmakeset-upquickandeasy

Electroniccalibration

Atwo-pointlinearcalibrationensuresaccuratedatareportinginmmHg

Pre-amplifiedanalogoutputs

Canbeintegratedwithvirtuallyanydataacquisitionsystem(5Vrangeminimum)withouttheneedforadditionalamplification

Twopressureinputs

PermitsuseofDual-PressureCathetersortwoSinglePressureCatheterssimultaneously

Calculatepressuregradientsandpulse-wavevelocity

Systemintegration

InterfacetheSP200withothermeasurementdevicessuchastransit-timeflow,echocardiography, andsonomicrometrysystems(additionalcablesmayberequired)

ESSENTIAL DESIGN ELEMENTS OF SCISENSE CATHETERSSolid-statepressuresensor

Sensorisside-mountedandrecessedwithinhousingforprotectionandsmoothnavigationwithinvessels

Ventedtoatmosphere,allowingforbarometricreference

Highlysensitive-accuratelyrespondstominuteandrapidchangesinpressure

Tubingmaterials

Idealbalanceofflexibilityandrigidityallowsforeasyinsertionandmaneuverability

Smoothandwillnotfrayovertime-noirritationtovesselliningorvalves

Tipoptions-straight,curved,angled,andpigtailoptionsareavailabletoassistwithnavigationandplacement





www.transonic.com


PressurePressure

LIMITED WARRANTY & RECALIBRATION Transonicwarrantsforaperiodoftwo(2)yearsfromdateofshipmentthatSP200hardware,cables,powersupply,and

associatedhardwareaccessories,purchasedthroughTransonicoritsdulyappointeddistributororlicensedrepresentative,arefreefromdefectsthatresultfromfaultymaterialorworkmanshipbyTransonic.

Transonicwarrantsforaperiodoftwelve(12)monthsfromdateofshipmentthatCatheterslessthantwoFrench(2F)arefreefromdefectsthatresultfromfaultymaterialorworkmanshipbyTransonic.WarrantyisonlyvalidifCathetersarepurchasedthroughTransonicoritsdulyappointeddistributororlicensedrepresentative.

TheTransonicwarrantydoesnotapplyto:defectscausedbyabuse,neglectormisuse;damageduetoaccidentorcasualty;orunauthorizedalterationsbyanyoneotherthanTransonicoranappointedrepaircenter.

TheBuyerpaysshippingchargestotheTransonicplantorrepaircenter;Transonicwillpayforreturnshipmentcharges.

Ifmeasurementaccuracyisquestionedduringthetwoyearwarrantyperiod,TransonicshallinspectandrecalibrateSP200hardwarefreeofchargeprovidedthattheBuyerpaysforallshippingcharges.

CATALOG # NAME DESCRIPTION

FFS-095-DP01SP200 Pressure Foundation System

Includes all items below as a complete system:Control Unit, Power Supply, Power Cord, (2) HDMI catheter cables, (2) BNC-BNC cables

FP095B SP200 Pressure Control UnitControl unit for operation of all Scisense Pressure Catheters.Provides two (2) pre-amplified pressure signals via BNC output.

FS-PCM50UT04 XP Power Supply Medical class isolated power supply.

FC-PCNA* North American Power CordConnects XP power supply to power source. *Alternate country specific power cords are available - please specify when ordering.

FC-HDMI-V3Scisense HDMI Catheter Cable (30AWG - 10 FT)

Connects all Scisense Pressure or PV Catheters to SP200 or ADV500 control units (not for use with older legacy units).

FC-BNC-6 BNC-BNC Cable (6ft) BNC cable for connection of control unit to most data acquisition systems.

Ordering Information

Pressure-Volume

Pressure-Volume Measurement System for Cardiac Function Research in All Sizes of Hearts

Pressure-Volume

Includes Conductance and improved Admittance volume mode operation

True volume in real-time when using Admittance mode

Variable Segment Length (VSL) Catheters ensure proper fit and accurate results

Compatible with virtually any data acquisition system and software package

Scisense ADV500

RPV-1-fly-A4 Rev. A 2/13

Pressure-VolumePressure-Volume

In the realm of heart function and hemodynamics, scientists have historically relied on systemic blood pressure, blood flow, and ventricular pressure to report changes in heart performance.

Applications in this field of study include phenotyping, the study of drugs and their possible cardiac-protective effects, and the genetic/environmental factors linked to heart disease.

Advancing the limits of science, we strive for better understanding, better treatments, and ultimately possible cures for heart disease. To achieve this goal researchers need to quantify performance, including subtle changes in contractility, elastance, power, energetics and efficiency. Fortunately, there is an answer to this demand - the Pressure-Volume Loop.

By plotting ventricular pressure and volume in the XY plane, a researcher can study individual cardiac cycles or a family of loops. Ejection fraction, cardiac output, max & min dP/dt, stroke volume, and tau are just a small example of the many parameters that can be reported. Additionally, valuable measurements of contractility and elastance are obtained by creating pressure-volume relationship curves. Derived only by PV Loop analysis, these relationships are crucial to cardiovascular studies to generate a full report of heart function.

Simply put, PV loops are essential in the study of hemodynamics and cardiac function.

Why PV Loops?

ADV500 Design & Technology

SOFTWARE MODULESFlexibility is important. This is why the ADV500 has been developed with individualized options for animal operation mode and volume measurement process. This design allows researchers to have a customized system specific to their needs and budget.

There are 3 software modules available, all designed to improve system accuracy and user experience over the basic conductance operation mode:

Rodent Admittance Module

Large Animal Admittance Module

Variable Segment Length (VSL) Catheter Module

ADMITTANCE VOLUME... THE NEW GOLD STANDARDAdmittance is our patented technology for calculating absolute volume in real-time.

Part 1: Automatic Isolation of the Blood Signal

Using a traditional PV Catheter inserted into the ventricle, Admittance allows for the detection of overall conductance and parallel conductance coming from the surrounding muscle tissue. We call this muscle measurement Phase. This is extremely important because the muscle contribution changes during the cardiac cycle and during the course of an experiment - therefore, it must be removed in order to accurately report volume. With parallel conductance automatically quantified, the system can isolate the blood signal.

Part 2: Conversion to Volume

Taking things one step further, the ADV500 on-board processor uses Weis equation1 to convert the isolated blood signal to true volume: the result is a real-time streaming signal presented in L or mL. When paired with an appropriate data acquisition system, researchers can view PV loops and calculate hemodynamic reports in real-time. This allows you to confirm quality data and start analysis at the bench-top, ultimately increasing throughput.

VARIABLE DESCRIPTION

ESP End-Systolic Pressure

EDP End-Diastolic Pressure

ESV End-Systolic Volume

EDV End-Diastolic Volume

HR Heart Rate

Max dP/dt Maximum Derivative of Pressure

Min dP/dt Minimum Derivative of Pressure

Max dV/dt Maximum Derivative of Volume

Min dV/dt Minimum Derivative of Volume

CO Cardiac Output

EF% Ejection Fraction

SV Stroke Volume

SW Stroke Work

Ea Arterial Elastance

maxPwr Maximum Power

plPwr Preload Adjusted Power

Eff Efficiency

PE Potential Energy

PVA Pressure-Volume Area

ESPVR End-Systolic PV Relationship

EDPVR End-Diastolic PV Relationship

PRSW Preload Recruitable Stroke Work

E(t) Time-Varying Elastance

Tau Isovolumic Relaxation Constant

PRESSURE-VOLUME LOOP MEASUREMENTS

BASELINE PRESSURE-CONDUCTANCE DATA FROM A HEALTHY MOUSE

In Conductance mode, the system outputs two signals: Pressure (mmHg) and Magnitude (S or mS).

Magnitude data can be converted to volume using traditional calibration techniques, which include a hypertonic saline injection to remove parallel conductance, and the use of Baans equation1 to convert data to volume units.

BASELINE PRESSURE-VOLUME DATA FROM A HEALTHY MOUSE

In Admittance mode, the system outputs four signals: Pressure (mmHg), Volume (L or mL), Phase (degrees), and Magnitude (S or mS).

No post processing is required to obtain accurate volume. In addition, researchers can view the Phase signal to confirm proper and consistent Catheter location in the center of the ventricle.

HIGH-RESOLUTION ECHO COMPARISON FROM A HEALTHY MOUSE

On the far left, XY plots show Admittance (blue) and Conductance (black) PV loops in two different scenarios: central Catheter position (top) and off-center position (bottom). Vertical dashed lines show end-systole and end-diastole, as measured by high-resolution echo. In both scenarios, Admittance data better correlates with echocardiography.

CORRELATION WITH MRI IN SHAM AND HF PIGS

Bland Altman plots show strong correlation between MRI volumes and Admittance volume measurements in sham pigs (blue dots) and pigs following 2 hour myocardial infarction by left circumflex coronary artery occlusion (red dots).

Features & Benefits

Suitable for use in a variety of animal models and different heart sizes

Provides the flexibility needed to customize your research program

Offers both traditional conductance and improved Admittance Volume calculation modes

Get the latest, most accurate PV loop technology while having access to traditional methods

Continue to collaborate with researchers even if they have older equipment

Variable Segment Length (VSL) Catheters provide flexibility

Ensures an accurate fit for larger hearts and dilation studies

One Catheter is suitable for a wider range of applications and animal types

Pre-amplified analog outputs (Pressure, Volume, Phase, Magnitude, Pressure 2)

Can be integrated with virtually any data acquisition system ( 5V range minimum)

Includes Additional Volume (AdVol) Admittance software

Recalculate volume while having control over the coefficients in Weis equation

Digital controls and LCD display

Intuitive menu design makes set-up and system use easy

Key system settings are displayed during acquisition to keep the researcher informed

Two pressure inputs

Acquire PV loops and blood pressure simultaneously via two Catheters

Calculate pressure gradients or pulse-wave velocity

The ADV500 system is both effective and reproducible in measuring LV function and dysfunction in the mouse, without the need for complicated interventions to calibrate the measurements.

James E. Clark Ph.D, Kings College London

This system is easy to use and provides clear and concise control features. The ability to generate true-volume in real time makes the system invaluable.

Pamela Lucchesi Ph.D, Nationwide Childrens Hospital

Pressure-VolumePressure-VolumePressure-Volume

LIMITED WARRANTY & RECALIBRATION Transonic warrants for a period of two (2) years from date of shipment that ADV500 hardware, cables, power supply, and

associated hardware accessories, purchased through Transonic or its duly appointed distributor or licensed representative, are free from defects that result from faulty material or workmanship by Transonic

Transonic warrants for a period of twelve (12) months from date of shipment that Catheters less than two French (2F) are free from defects that result from faulty material or workmanship by Transonic. Warranty is only valid if Catheters are purchased through Transonic or its duly appointed distributor or licensed representative.

The Transonic warranty does not apply to: defects caused by abuse, neglect or misuse; damage due to accident or casualty; or unauthorized alterations by anyone other than Transonic or an appointed repair center.

The Buyer pays shipping charges to the Transonic plant or repair center; Transonic will pay for return shipment charges.

If measurement accuracy is questioned during the two year warranty period, Transonic shall inspect and recalibrate ADV500 hardware free of charge provided that the Buyer pays for all shipping charges.

Ordering Information

REFERENCE1. Porterfield JE, Kottam AT, Raghavan K, Escobedo D, Jenkin JT, Larson ER, Trevino RJ, Valvano JW, Pearce JA, Feldman MD. Dynamic correction for parallel

conductance, Gp, and gain factor, , in invasive murine left ventricular volume measurements. J Appl Physiol 107: 16931703, 2009.





www.transonic.com

Transonic Systems Inc. is a global manufacturer of innovative biomedical measurement equipment. Founded in 1983, Transonic sells gold standard transit-time ultrasound flowmeters and monitors fo

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