Nature | www.nature.com | 1
Article
Reversing a model of Parkinson’s disease with in situ converted nigral neurons
Hao Qian, Xinjiang Kang, Jing Hu, Dongyang Zhang, Zhengyu Liang, Fan Meng, Xuan Zhang, Yuanchao Xue, Roy Maimon, Steven F. Dowdy, Neal K. Devaraj, Zhuan Zhou, William C. Mobley, Don W. Cleveland & Xiang-Dong Fu ✉
In the format provided by the authors and unedited
Supplementary information
https://doi.org/10.1038/s41586-020-2388-4
Nature | www.nature.com/nature
anti-Actb
Figure 1 panel c
50KD
37KD
70 KD
Figure 1 panel d
anti-Actb
anti-PTB
0d 2d 4d 7d 14d 21d
0d 2d 4d 7d 14d 21d
0d 2d 4d 7d 14d 21d
Short exposure
Long exposure
50KD
37 KD
Figure 7 panel a
37 KD
mAstrocyte+PTB-ASO
1# 2# 3# 4# 5#
Note: Red boxes indicate thebands usedfor mainfigure-4.
Short exposure
Long exposure
anti-ACTB
anti-BRN2
70 KD
50KD
37 KD
ExtendeddataFigure 1 panel c
anti-Actb
anti-PTB
0d 2d 4d 7d 14d 21d
0d 2d 4d 7d 14d 21d
0d 2d 4d 7d 14d 21d
anti-ACTB
anti-nPTB
Short exposure
Long exposure
ExtendeddataFigure 1 panel eHDF
70KD
50KD
37KD
70 KD
50 KD
37 KD
ExtendeddataFigure 1 panel ecortical astrocyte
0d 2d 4d 7d 14d 21d
0d 2d 4d 7d 14d 21d
0d 2d 4d 7d 14d 21danti-Actb
anti-nPTB
Short exposure
Long exposure
72 KD
52KD
42 KD
0d 2d 4d 7d 14d 21d
anti-ACTB
anti-nPTB0d 2d 4d 7d 14d 21d
anti-ACTB
anti-PTB
ExtendeddataFigure 1 panel ehuman astrocyte
70 KD
50KD
37 KD
70 KD
50KD
37 KD
ExtendedDataFig.18panel c
anti-Actb
anti-TH