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Lecture 8 Slides

First, a little cell biology….

fig 19-1

The mitochondrion (plural mitochondria)

fig 19-1

Krebs cyclefeatures andfunctions…

fig 16-13

making citrate asymmetric

Stereochemistry of isocitrate production

box 16-3

misprint

Stereochemistry of isocitrate production

box 16-3

misprint

Stereochemistry of isocitrate production: corrected

box 16-3

correction

Stereochemistry of isocitrate production

but…. WHY?

Why isn’t this reaction random?

Stereochemistry of isocitrate production

Alexander “Sandy”Ogston

this result implies enzyme has asymmetric “landing site”

this result implies enzyme has asymmetric “landing site”

Stereochemistry of isocitrate production

box 16-3

Stereochemistry of isocitrate production

box 16-3

implies enzyme has asymmetric “landing site”

but…

Stereochemistry of isocitrate production

box 16-3

also means the substrate, citrate, was MADE asymmetrically!

is this hard to imagine?box 16-3

CH2-COO-

fig 16-8

Citrate synthase…

Krebs cycle and energy revenue

fig 16-13

Krebs cycle and energy revenue

table 16-1

fig 16-14

a molecular antique fromthe days whenbefore it wasa cycle…

a fermentation!

NADH

FADH2

the amphibolic Krebs cycle…

fig 16-15

the amphibolic Krebs cycle: anapleurotic reactions

fig 16-15

the anaplerotic reactions

table 16-2

the anaplerotic reactions

table 16-2

an example: pyruvate carboxylase

table 16-2

Biotin! activation of CO2 in pyruvate carboxylase

fig 16-16

fig 16-16

Biotin! activation of CO2 in pyruvate carboxylase

fig 16-16

Biotin! activation of CO2 in pyruvate carboxylase

fig 16-16

Biotin! activation of CO2 in pyruvate carboxylase

fig 16-16

Biotin! activation of CO2 in pyruvate carboxylase

fig 16-16

Biotin! activation of CO2 in pyruvate carboxylase

Regulationof the Krebscycle at multiple reactions…

fig 16-18

three bigenergy drops areregulated

Regulationof the Krebscycle...

three bigenergy drops areregulated

fig 16-18

Regulationof the Krebscycle...

three bigenergy drops areregulated

fig 16-18

Regulationof the Krebscycle...

three bigenergy drops areregulated

fig 16-18