R.LIA ISWARA MS,SpMKDept of MicrobiologyDept of Microbiology

FK USU

DNA VIRUS:1. Herpes simplex virus (HSV)2. Cytomegalo virus (CMV)3. Papiloma virus (HPV)4. B19 virus (Parvo virus) (B19)5. Hepatitis B virus (HBV)

Virus Diseases Properties Transmission

Clinical findings

Laboratory diagnosis 

Treatment

Herpes  ‐Genital  ‐DNA ‐sexual  ‐vesicular  Isolation: AcycloviSimplex virus‐2 (HSV)

Herpes

‐Neonatal Herpes

‐Double stranded

intercourse

‐ Contact with 

eruption

‐jaundice

‐Vesiclefluid

‐skin swab

r

Herpes‐100 nm

with herpetic lessions in the birth 

‐HepatosplenoMegali

‐skin swab

Inoculation:‐tissue 

canal (during birth)

‐skin lesions

cultures

Serology:  Nt, CF, 

‐encephalitis

Nt, CF, ELISA

Virus Diseases Properties

Transmission

Clinical findings

Laboratory diagnosis

Prevention  & control

Cytomegalovirus (CMV)

Cytomegalic‐ Inclusion‐Disease (CID)

DNA ‐Transplacentally in utero

‐Mental retardation‐Jaundice‐Hepato

Isolation:‐urine‐throat swab

‐Ganciclovir‐Acyclovir(CMV) (CID) ‐Hepato

spleenomegali‐Blood 

Inoculation:‐CPE, 

clovir

dyscrasia(thrombocytopeniahaemolytic

swollen  cell

‐Serology:Nt, CF, haemolytic

anemia‐MicrocephaliM t

Nt, CF, ELISA

‐Motorneurondiseases

Virus Diseases Properties Pathogenesis

Clinical findings

Laboratory diagnosis

Prevention  & control

Parvovirus( B 19)

‐erythemainfectiosum (fifth disease) / 

‐DNA‐Singlestranded 

‐Trans placental‐Respiratory route

‐Hydropsf etalis/Fetal death (due 

‐Isolation:Throat swab

‐PCR disease) / ”slapped cheek”‐Aplastic

ry route‐Blood trans fusions 

death (due to fatal anemia) 

‐PCR 

anemia‐ Hydropsfetalis and fetal death fetal death 

Virus Diseases Properties

Transmission

Clinical findings

Laboratory diagnosis

Prevention  & control

Human PapilomaVirus  (HPV)

‐Genital Condyloma

‐Cervical

‐DNA‐Double strandedCircular

‐ Sexual intercourse

‐differentiation of epithelial

‐PCR No vaccine

(HPV) Cancer (type 16,18,31,33,35,39

Circular‐ 55 nm

epithelialcell

Virus Diseases Properties

Transmission

Clinical findings

Laboratory diagnosis

Prevention  & control

Hepatitis B Virus (HBV)

‐Hepatitis B

Incubation period:

‐ds DNA ‐Parenteral‐Sexual intercourse‐trans 

jaundice Serology:HBs Ag

‐Vaccine:Recombivax‐Hyper period:

50‐180 days /avg, 60‐90‐Chronic 

‐trans placental

‐Hyper immune globulin:Hepatitis 

carriers: Hepatocellular Carcinoma

B immunogloblin

Carcinoma

THANK YOU

R.LIA ISWARA MS,SpMKDept of MicrobiologyDept of Microbiology

FK USU

RNA VIRUS:1. Rubivirus (rubella virus)2. Human Immunodeficiency virus (HIV)3. Measles virus4. Mumps virus

Virus Diseases Properties Transmission

Clinical findings

Laboratory diagnosis 

Prevention

‐Rubellavirus (Rubivirus)

‐Rubella (German measles)‐Incubati

‐RNA ‐50‐75nm

‐Respiratory‐trans placentally

‐Triad Congenital defect:cataract  

Serology: HI testIgG rising titer

Vaccine:Life attenuated  virusvirus) ‐Incubati

on period: 14‐21 days

cataract, nerve deafness, cardiac 

titerIgMCFT

ed  virus

abnormality (The Rubella Syndrome)Syndrome)

Virus Diseases Properties Transmission

Clinical findings

Laboratory diagnosis 

Prevention

‐HIV ‐AIDS ‐ ssRNA ‐Sexualcontact‐Trans placentally

‐primaryinfection(viremia :8‐12 weeks)

‐Serology:ELISA,‐Western blot

No vaccine is availableplacentally weeks)

‐clinical latency (10 years)

blot‐Rapid test

available

‐AIDS: immunosuppression, chronic chronic diarrhea, weight loss, Kaposi 

 sarcoma, lymph adenopathy. (2‐3 years)

Virus Diseases Properties Transmission

Clinical findings

Laboratory diagnosis 

Prevention

‐Measles ‐Measles ‐RNA  ‐Respiratory ‐Fetal death Isolation: in  Measles ‐Rubeola‐Morbili

‐Lifelong 

‐120‐250nm ‐trans placentally

‐Fever, conjunctivitis, cough, kopling

cell cultureSerology : rising titer greater than 

vaccine, one dose  s.c.

‐Lifelong immunity

‐Incubatio

koplingspots in buccal

greater than 4 fold Life 

attenuated 

n period: 10‐14 days

vaccine

Virus Diseases Properties Transmission

Clinical findings

Laboratory diagnosis 

Prevention

‐Mumps  ‐Mumps  ‐RNA  ‐ Droplet  ‐Fever,  Serology: Mumps virus parotitis

epidemica‐Lifelong immunity

‐110‐170 nm‐Envelope particles

infection malaise, anorexia, tender swelling 

CFT Culture:saliva, spinal fluid  

vaccine,

Life attenuatimmunity

‐Maternal antibody passes the

swelling Parotid gland

spinal fluid, urinr

attenuated vaccine

placenta, immunity first 6 month of month of life.‐Incubation period: 8  d18‐21 days