Rm path. (rabi 12 13)

A. K. MishraJr. Scientist -cum- Asstt. Prof.

Tirhut College of Agriculture (RAU)

Dholi, Muzaffarpur, BIHAR

Research Highlights of

AICRP on Rapeseed & Mustard Pathology

Name of the experiment: Screening of Brassica AVT-1 & AVT-2 entries against different diseases under Natural and Artificial conditions.

Technical Programme:No. of test entries : 31Plot Size : 3m long single rowSpacing : 30cm X 10 cmReplication : 2Date of sowing : 09.11.2012Disease observation : % severity recorded at (90-100 DAS)

Year of start: 2012

No. of years conducted: 1 Year

Brief research highlights:

Alternaria Blight (Alternaria brassicae)

Symptoms Disease reaction

Natural condition Artificial condition

Leaves Pods Leaves Pods No lesion Immune - - - -

<5% leaf/pod covered by lesion HR - - - -5-10% leaf/pod covered by lesion

R - - - -

11-25% leaf/pod covered by lesion

MR - 17 - 5

26-50% leaf/pod covered by lesion

S 27 14 9 24

>50% leaf/pod covered by lesion HS 4 - 22 2

Disease intensity (%) 30.00-61.00 15.50-47.50 34.00-75.00 19.50-51.50

Yellow Sarson (Susceptible check) showed HS reaction.

Plan of work for the ensuing season: - To be continued with new entries.

Recommendation based on concluded trial: - Nil

New area of research identified and new research projects formulated; if any with details:- Nil

Symptoms Disease reaction Natural condition Artificial conditionNo disease R 24 15Upto 25% stem girdled MR 4 325-50% stem girdled MS 3 1151-75% stem girdled S - 2>75% stem girdled HS - -

Disease intensity (%) 7.50-32.50 12.50-65.00

Sclerotinia Stem Rot (Sclerotinia sclerotiorum)


Name of the experiment: Uniform Disease Nursery (UDN) trial for major disease of rapeseed- mustard under natural condition.

Technical Programme:No. of test entries : 54Plot Size : 3m long single rowSpacing : 30cm X 10 cmReplication : 2Date of sowing : 09.11.2012Disease observation : % severity recorded at (90-100 DAS)

Year of start: 2012


Symptoms Disease reaction


White Rust(Albugo candida)

Leaves PodsNo lesion Immune - - 9

<5% leaf/pod covered by lesion HR - - 125-10% leaf/pod covered by lesion R - - 2811-25% leaf/pod covered by lesion MR - - 526-50% leaf/pod covered by lesion S 42 53 ->50% leaf/pod covered by lesion HS 12 1 -

Disease intensity (%) 33.00-67.50 30.50-56.00 1.00-17.50


Symptoms Disease reaction No disease R 43Upto 25% stem girdled MR 325-50% stem girdled MS 851-75% stem girdled S ->75% stem girdled HS -

Disease intensity (%) 17.50-35.00



Plan of work for the ensuing season: - To be continued with new entries.Recommendation based on concluded trial: - NilNew area of research identified and new research projects formulated; if any with details:- Nil

Name of the experiment: National Disease Nursery (NDN) for Alternaria blight under artificial inoculated condition. Technical Programme:No. of test entries : 23Plot Size : 3m long single rowSpacing : 30cm X 10 cmReplication : 2Date of sowing : 09.11.2012Disease observation : % severity recorded at (90-100 DAS)

Year of start: 2012No. of years conducted: 1 Year

Symptoms Disease reaction Leaves Pods

No lesion Immune - -<5% leaf/pod covered by lesion HR - -

5-10% leaf/pod covered by lesion R - -11-25% leaf/pod covered by lesion MR - -26-50% leaf/pod covered by lesion S 12 23>50% leaf/pod covered by lesion HS 11 -


Alternaria Blight (Alternaria brassicae)Brief research highlights:



Name of the experiment: National Disease Nursery (NDN) trial for Sclerotinia Stem Rot under artificial condition. Technical Programme:No. of test entries : 16Plot Size : 3m long single rowSpacing : 30cm X 10 cmReplication : 2Date of sowing : 09.11.2012Disease observation : % severity recorded at (90-100 DAS)


Symptoms Disease reaction No disease R 3Upto 25% stem girdled MR 525-50% stem girdled MS 651-75% stem girdled S 2>75% stem girdled HS -

Disease intensity (%) 12.50-55.00





Name of the experiment: Screening of IVT entries of Brassica against Alternaria Blight under natural condition.Technical Programme:No. of test entries :116Plot Size : 3m long single rowSpacing : 30cm X 10 cmReplication : 2Date of sowing : 08.11.2012Disease observation : % severity recorded at (90-100 DAS)


Symptoms Disease reaction Leaves Pods

No lesion Immune - -<5% leaf/pod covered by lesion HR - -

5-10% leaf/pod covered by lesion R - -11-25% leaf/pod covered by lesion MR - -26-50% leaf/pod covered by lesion S 60 113>50% leaf/pod covered by lesion HS 56 3






Name of the experiment: Integrated Disease Management of Alternaria Blight of mustard.Technical Programme:Design: RBD Plot size: 5m X 3m Replication : 3 Cv.: VarunaBasal soil application of N100 P40 K40 per ha Date of sowing : 08.11.2012Year of start: 2010No. of years conducted: 3 Year Treatments

T1 Seed treatment with Trichoderma harzianum @ 10g/ kg seed followed by foliar spray of Pseudomonas fluorescences (oil based) @10 ml /l water at the flower initiation stage.

T2 ZnSO4 soil application @15kg/ha + Borax soil application @10kg/ha + Sulphur soil application @20kg/ha.

T3 Removal of three lower leaves

T4 Seed treatment with mixture of ipridione +carbendazim (1:1) @ 2g/kg seed followed by 2 foliar sprays of carbendazim + [email protected]%

T5 ZnSo4 soil application @15kg/ha+ Borax soil application @10kg/ha + Sulphur soil application @20kg/ha + 2 foliar sprays of carbendazim + [email protected]%

T6 ZnSO4 soil application @15kg/ha + Borax soil application @10kg/ha + Sulphur soil application @20kg/ha + foliar spray of Pseudomonas fluorescence (oil based) @10 ml /l water

T7 Removal of three lower leaves + foliar spray of Ridomil MZ72 WP@ 0.2%T8 Seed treatment with mixture of ipridione +carbendazim (1:1) @ 2g/kg seed followed by removal of

three lower leavesT9 Seed treatment with propiconazole@ 0.1 % followed by its foliar spray @0.1%

T10 Control

Brief research highlights: All the treatments significantly controlled the severity of AB over control. Lowest disease intensity of 30.47% and 22.80% was recorded on leaves and pods respectively with highest yield of 1208 Kg/ha. in treatment T7 (Removal of three lower leaves + foliar spray of Ridomil MZ 72 WP@ 0.2%) but highest ICBR of 1:5.21 was recorded in treatment T9 (Seed treatment with Propiconazole@ 0.1 % followed by its foliar spray @0.1%.Plan of work for the ensuing season: - concludedRecommendation based on concluded trial: Seed treatment with Propiconazole @0.1% followed by its foliar spray @0.1% at flower initiation stage can be recommended to manage Alternaria blight (Alternaria brassicae) disease of mustard. New area of research identified and new research projects formulated; if any with details:- New experiment formulated

Treatments Intensity on leaves

(%)

Intensity on pods

(%)

Yield (Kg/ha)

ICBR

T1 Seed treatment with Trichoderma harzianum @ 10g/ kg seed followed by foliar spray of Pseudomonas fluorescences (oil based) @10 ml /l water at the flower initiation stage.

52.47 41.97 847 1:(-)0.14

T2 ZnSO4 soil application @15kg/ha + Borax soil application @10kg/ha + Sulphur soil application @20kg/ha.

50.97 43.87 850 1:(-)0.05

T3 Removal of three lower leaves. 47.83 37.00 958 1:1.12T4 Seed treatment with mixture of Ipridione +Carbendazim (1:1) @ 2g/kg

seed followed by 2 foliar sprays of Carbendazim + [email protected]%45.07 36.50 1027 1:1.99

T5 ZnSo4 soil application @15kg/ha+ Borax soil application @10kg/ha + Sulphur soil application @20kg/ha + 2 foliar sprays of Carbendazim + [email protected]%

33.10 28.10 1117 1:(-)0.45

T6 ZnSO4 soil application @15kg/ha + Borax soil application @10kg/ha + Sulphur soil application @20kg/ha + foliar spray of Pseudomonas fluorescence (oil based) @10 ml /l water

42.33 37.17 1033 1:(-)0.29

T7 Removal of three lower leaves + foliar spray of Ridomil MZ 72 WP@ 0.2%

30.47 22.80 1208 1:2.65

T8 Seed treatment with mixture of Ipridione + Carbendazim (1:1) @ 2g/kg seed followed by removal of three lower leaves

50.83 44.23 863 1:(-)0.30

T9 Seed treatment with Propiconazole@ 0.1 % followed by its foliar spray @0.1%

38.50 31.33 1075 1:5.21

T10 Control. 59.60 48.20 822CD(p=0.05)

8.53 7.51 137.71

CV (%) 11.05 11.80 8.21SEm(±) 2.87 2.52 46.34

Table: Integrated Disease Management of Alternaria Blight of Mustard.

Evaluation of fungicides against major diseases of rapeseed-mustard.

Design-RBD; Plot size- 5 m x 3 m; Replications- 03; Variety-Varuna

Seed should be treated with Apron 35 1. SD (6g/kg seed) before sowing in all the treatments.First and second spray should be applied 45 and 60 DAS, respectively. Treatments:T1= Spray of Mancozeb (0.2%)

T2= Spray of Metalaxyl 8%+ Mancozeb 64% WP (0.2%)

T3= Spray of Hexaconazole 25 EC (0.05%)

T4= Spray of Difenconazole 25 EC (0.05%)

T5= Spray of Propiconazole 25 EC (0.05%)T6=Seed treatment with Propiconazole@ 0.1 % followed by its foliar spray @0.1%

T7= T1 followed by T2

T8= T1followed by T3


T10= T1followed by T5


T12= Water spray (Control)

(New Expt.)Technical Programme:

Observations: Disease Incidence/ IntensityYield/plot (kg) Yield (t/ha)

Name of the experiment: Disease monitoring on Farmers’ field.

Year of start: 2012



Plan of work for the ensuing season: - To be continued.

Recommendation based on concluded trial: Nil

New area of research identified and new research projects formulated; if any with details:- Nil

Disease recorded Samastipur Muzaffarpur


0-15%Mean= 2.25%

0-10%Mean= 1.40%

White Rust(Albugo candida)

0-15%Man = 1.54%

0-5%Mean = 1.22%

Sclerotinia Stem Rot(Sclerotinia sclerotiorum)

0-80%Mean= 6.82%

0-75%Mean = 8.97%

ALTERNARIA BLIGHT

SCLEROTINIA ROT

WHITE RUST

Date post:	14-Jul-2015
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Rm path. (rabi 12 13)

Technology