Roscoff Culture Collection

Souchothèque de Bretagne

Station Biologique de RoscoffScientist in charge: D. Vaulot (DR1 CNRS)

Curator: Florence Le Gall (AI CNRS)

Number of unique strains : 516

Backup cultures not taken into account

Class CountBacteria Cyanophyceae 149Chlorophyta Chlorophyceae 9

Trebouxiophyceae 30Prasinophyceae 100

Chlorarachniophyta Chlorarachniophyceae 4Cryptophyta Cryptophyceae 5Rhodophta Bangiophyceae 4Haptophyta Pavlophyceae 2

Prymnesiophyceae 30Stramenopiles Bacillariophyceae 20

Bolidophyceae 8Eustigmatophyceae 4Pelagophyceae 11Chrysophyceae 6Dictyochophyceae 13Pinguiophyceae 5Fucophyceae 1Bicosoecid 5Heterotrophic Stramenophiles 3

Alveolates Dinophyceae 8Ciliés 1

Others Euglenophyceae 1Euglenozoa 1Telonema 3Unknown 93

Class CountBacteria Cyanophyceae 149Chlorophyta Chlorophyceae 9

Trebouxiophyceae 30Prasinophyceae 100

Chlorarachniophyta Chlorarachniophyceae 4Cryptophyta Cryptophyceae 5Rhodophta Bangiophyceae 4Haptophyta Pavlophyceae 2

Prymnesiophyceae 30Stramenopiles Bacillariophyceae 20

Bolidophyceae 8Eustigmatophyceae 4Pelagophyceae 11Chrysophyceae 6Dictyochophyceae 13Pinguiophyceae 5Fucophyceae 1Bicosoecid 5Heterotrophic Stramenophiles 3

Alveolates Dinophyceae 8Ciliés 1

Others Euglenophyceae 1Euglenozoa 1Telonema 3Unknown 93

Culture characterization

Done PlannedGene sequences 18S partial SBR 142 19

18S full SBR 8216S full Nick 50psbA Oded Beja 17Silicon transporter Pascal Lopez 12

Pigments HPLC SBR/Mikel 119Microscopy LM SBR 232

EM Wenche/SBR 31

Done PlannedGene sequences 18S partial SBR 142 19

18S full SBR 8216S full Nick 50psbA Oded Beja 17Silicon transporter Pascal Lopez 12

Pigments HPLC SBR/Mikel 119Microscopy LM SBR 232

EM Wenche/SBR 31

Planning for coming months

TEM on heterotrophic flagellates from Blanespriority to the smallest ones

Purify cultures which contain contaminants such as heterotrophic flagellates

Purification on plate (0,3% agar)

Serial dilutions (1 cell/tube) Coccoids

Flagellates

Continue to sequence 18S rDNA RCC strains

Indian Ocean cruise planned in may 2003:• Screening of precultures• Isolation of new strains

Marine bacteria (hyperthermophiles)LMBE (IFREMER) G. Barbier

Centralized data base accessible through webCentralized data base accessible through web

Fungi and bacteria related to food scienceESMISAB (UBO) Y. Tirilly

PicoplanktonSBR (CNRS, UPMC) D. Vaulot

Marine bacteria (hyperthermophiles)LMM (UBO) D. Prieur - coordinator

4000 à 5000 strains4000 à 5000 strains

DiatomsISOMER (U Nantes) J.M. Robert

Microalgae of economical interestIFREMER (A. Muller-Feuga)

Microalgae(Coccoilthophorids..)

Algobank (U Caen) C. Billard

MicroalgaeENS Paris J.Houmard