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EXPERTISE, CAPABILITIES & INFRASTRUCTURES
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BBIIOOPPHHYYSSIICCAALL CCHHAARRAACCTTEERRIISSAATTIIOONN OOFF PPRROOTTEEIINNSS –– PPrrooff.. VVííccttoorr MMuuññoozz
EXECUTIVE SUMMARY
From basic research, the group aims to contribute with advances which help to understand protein behavior since 2000. Such as it is known, this protein behavior is the cause of many diseases (Alzheimer´s, Parkinson´s, cystic fibrosis, and many forms of cancer). The group focuses on investigations around Conformational and Functional Behavior of Proteins through a multidisciplinary approach, borrowing
concepts and methodologies from condensed matter physics, physical chemistry, molecular spectroscopy, computer science, protein chemistry, and molecular and cell biology.
During last years, the group has provided experimental evidence for a mechanism of protein folding called as “downhill folding”. Dr. Victor Muñoz has been pioneering various techniques, both computational and experimental, to study this mechanism as well as to gain insights into the general process of protein folding.
CURRENT RESEARCH THEMES OF THE GROUP
Specific projects currently developed by the group are:
♦ Exploration of protein folding ensembles
♦ Structure prediction and design through calculation of folding free energy surfaces
♦ Understanding downhill folding and testing the molecular rheostat hypothesis
♦ New concepts and approaches in protein aggregation and refolding
1. NMR of Protein BBL; 2. Energy Landscape of folding protein
SPECIFIC SKILLS
In the investigations there are employed kinetic techniques (stopped-flow, continuous flow, temperature-jump), steady state microscopy (fluorescence absorption, circular dichroism), nuclear magnetism resonance, single molecule studies and high performance computing to answer question concerning the intimate behavior of proteins.
From these techniques, our group is able to perform the
characterization of stability and kinetics of protein folding with the maximum accuracy that current technology allows.
We are able to perform:
♦ Characterization and understanding of secondary and folding protein structure.
♦ Studies on the effects of environmental conditions (salts, denaturants, pH, etc.) on proteins folding.
♦ Studies on protein aggregation and refolding through.
♦ Bioinformatic data management techniques. See more
COMPLEMENTARY SKILLS
♦ Molecular Biology Standard Techniques. ♦ Cell Biology Standard Techniques ♦ Protein Production
EQUIPMENT OR FACILITIES
OUR TEAM
Dr. Muñoz has been a member of the faculty of the Dpt. Chemistry & Biochemistry at the University of Maryland (USA)
since 2000 and has won the Searle Scholar Award, and the David and Lucille Packard Fellowship for Science and Engineering. He moved to Spain in 2007 as a professor at the Center for Biological Investigations of the CSIC at Madrid. He was elected to the European Molecular Biology Organization in 2009.
The further group´s personnel is formed by 6 doctors in different science fields (physics, chemistry and biology), 4 Ph.D. students that include skills such as pharmacy and
bioinformatics.
Click here to see selected publications
COLLABORATIONS & PARTNERSHIPS
Prodestech. Prodestech is a scientific consortium devised to make specific advancements towards the goals of Protein Design and Engineering technologies in a synergistic way wherein the group is the coordinator. The project is funded since 2009 by Spanish Ministry of Science & Technology through the Consolider Programme.
We are used to collaborate with many groups and companies worldwide in projects related with proteins.
We are looking for joining to consortiums or offer research or consultancy services interested in joining to projects where we could contribute with our know-how and technical skills on the conformational and functional behavior of proteins.
CONTACT
We are based on the Biological Investigations Center – CSIC. In case you were interested in our collaboration, please feel free to contact us at:
Principal Investigator: [email protected]
Tech Transfer: [email protected]
Tel: +34 91 837 31 12 Ext: 4389/4438
Fax: +34 91 536 04 32
c/ Ramiro de Maeztu, 9. 28040 Madrid. CIB-CSIC Lab 004
http://tmg.cib.csic.es/
PPRROOTTEEIINN EENNGGIINNEEEERRIINNGG && EENNEERRGGEETTIICCSS –– PPrrooff.. JJoossee MMaannuueell SSaanncchheezz--RRuuiizz
EXECUTIVE SUMMARY
The group has many years experience in the application of calorimetric approaches biological problems and in the study of the structure-energetics relationship in proteins as a basis for the application of protein engineering to biotechnologically relevant problems.
We aim to get closer to society through collaborations with public and private institutions devoted to applied research
and trough the transfer of the developed technology to the industrial sectors.
Mapping of E. coli thioredoxin 3D structure
CURRENT RESEARCH THEMES OF THE GROUP
The group is currently carrying out active research in the following topics:
♦ Analysis of irreversible protein denaturation and protein kinetic stability.
♦ Surface electrostatic design in protein engineering.
♦ Experimental determination of energy landscapes for
ultrafast protein folders.
♦ The molecular basis of misfolding and mistargeting diseases.
♦ The relationship between protein biophysics and protein evolution.
♦ “Resurrection” and experimental characterization of Precambrian proteins.
♦ Application of evolutionary approaches to protein engineering.
SPECIFIC SKILLS
The group has a strong background in the application of microcalorimetry to biological problems and also in biochemical kinetics, bioinformatics, bioenergetics, protein folding and protein engineering.
GENERAL AND COMPLEMENTARY SKILLS
Other expertise includes molecular biology and directed evolution.
EQUIPMENT OR FACILITIES
Capillary differential scanning calorimeters with autosampler units for enhanced throughput determination of protein stability
OUR TEAM
Dr. Jose Manuel Sanchez-Ruiz is Full Professor of Physical Chemistry at the University of Granada. He has published
about 100 scientific papers appearing cited 2742 in the Thompson ISI. He is currently a member of the Editorial Board of Biochemical Journal and previously was a member of the Editorial Board of Biophysical Chemistry for 7 years.
The group is formed by 5 doctors, 6 Ph.D students (5 chemists, 1 chemical engineer, 5 biologists) and 1 technician.
COLLABORATIONS & PARTNERSHIPS
The group has a broad expertise in collaborative work with research groups of prestige, including groups at Columbia University (single molecule analysis of enzyme catalysis), Johns Hopkins (protein electrostatics) Bergen (14-3-3 proteins) and NIH (ultrafast folders), as well as with the groups in the Prodestech consortium. We also collaborate in studies of protein engineering and related biotechnological
and biomedical applications with private companies, such as Noricum or Novozymes.
Prodestech. Prodestech is a scientific consortium devised to make specific advancements towards the goals of Protein Design and Engineering technologies in a synergistic way wherein the group participates actively. The project is funded since 2009 by Spanish Ministry of Science & Technology through the Consolider Programme.
We are open to make available our broad knowledge and multidisciplinary technical skills on proteins and specifically on protein refolding in any type of projects with the aim of going forward in the global knowledge at this field.
We are interested in joining to consortiums, offer research or consultancy services and, in general, taking part in projects where we can contribute with our know-how and technical skills.
CONTACT
We wish to offer our know-how and research services as CRO or simply to set up new partnerships in order to open new research lines within our knowledge field.
In case you were interested in our collaboration, please feel free to contact us at:
Principal Investigator: [email protected]
Tech Transfer: [email protected]
Tel: +34 958 24 31 89 Fax: +34 958 27 28 79
http://prodestech.cib.csic.es/members/group-2
SSYYNNTTHHEETTIICC MMIICCRROOBBIIAALL MMAACCRROOMMOOLLEECCUULLAARR AASSSSEEMMBBLLIIEESS –– PPrrooff.. RRaaffaaeell GGiirraallddoo
EXECUTIVE SUMMARY
Our group aims to contribute minimal microbial model systems for pre-clinical applications (screening and diagnostic tools) in the area of protein amyloidosis, which is related to many human degenerative diseases.
Over a decade, our basic research on proteins bearing Winged-Helix (WH) domains involved in replication initiation of bacterial plasmids and yeast chromosomes has allowed
us to unravel the molecular basis for a ligand (DNA)-promoted and chaperone (Hsp70)-modulated conformational remodeling, building a switch between functional activation or amyloid aggregation. We are now interested in the development of application such as microbial (bacterial and yeast)-based biosensors for the screening of small molecules capable to inhibit or control protein amyloidosis
CURRENT RESEARCH THEMES OF THE GROUP
Specific projects currently developed by the group are:
♦ Conformational activation and primers of synthetic protein and protein-DNA macromolecular complexes
♦ New microbial model systems for tackling protein amyloidosis.
More info at the group web.
SPECIFIC SKILLS
The group has expertise on in vivo studies (bacterial and yeast genetics), including fluorescence and immune-electron microscopies, and in vitro approaches such as biochemistry and biophysics of complex systems: protein assemblies, protein-DNA interactions or surface adsorption (using techniques such as electrophoresis, CD and fluorescence spectroscopies, protein and nucleic acid chemistry, electron microscopy, analytical centrifugation and light scattering)
♦ Screening of amyloidosis inhibitors and modulators in microbial systems.
♦ Engineering amyloid assemblies as novel nano-tools in vitro and as functional scaffolds in vitro.
♦ Synthetic biology of ligand-induced conformational switches (design of protein devices to artificially control DNA replication and amyloid assembly; protein arrays engineering; and feasibility assessment on microbial modules.
GENERAL AND COMPLEMENTARY SKILLS
♦ Cell Biology (bacteria and yeast)
♦ Molecular Biology
♦ Biophysics
EQUIPMENT OR FACILITIES
Apart from the equipment typical in a Molecular Biology laboratory (to handle with DNA and proteins), the group has access to the common facilities at CIB-CSIC, including electron and confocal microscopes, analytical ultracentrifuge, fluorimeter, spectropolarimeter, mass spectrometers, DNA and peptide synthesizers, plate readers, etc.
OUR TEAM
Dr. Rafael Giraldo is internationally recognized on the study of the Molecular Biology of bacterial plasmids and in the fields of protein-DNA interactions and Synthetic Biology.
The group is currently formed by 2 senior scientists, 3 postdoctoral researchers, 1 Ph.D. student and 1 technician.
Click here to see selected publications
COLLABORATIONS & PARTNERSHIPS
Inside the CIB we work in collaboration with several groups and fields: S. Moreno (immuno-cytochemistry/iEM), G. Rivas (cytomimmetic chemistry), V. Muñoz (FTIR spectroscopy), A. Romero (X-ray crystallography), E. de Alba (NMR) and O. Llorca (EM reconstruction of amyloid fibers).
Within Spain research sector we collaborate with: J.M. Valpuesta, CNB-CSIC (EM on chaperones), F. Moreno, CNB-CSIC, Madrid (AFM on amyloid fibers), P. Chacón, IQFR-CSIC, Madrid (in silico studies on amyloid fibers), F. De la Cruz, Cantabria U. (Engineering conjugative relaxases). We are an active partner of the PRODESTECH Consortium, project funded since 2009 by Spanish Ministry of Science & Technology through the Consolider Programme.
We also maintain synergistic collaborations along worldwide: Ariel Lindner, CRI-París, France (microfluidics), Igor Konieczny, IFB-Gdansk, Poland (Structure and assembly of Rep proteins), Ana Paula Ulian-Araújo, IFSC-Sao Paulo, Brasil (Septin amyloidosis) and Rosalba Lagos, U. Santiago, Chile (Microcin amyloidosis).
We are looking for joining to consortiums or offer research or consultancy services interested in joining to projects (i.e. our expertise fits perfectly on the 7FP´s call HEATLH.2013 Functional validation in animal and cellular models of genetic determinants of diseases and ageing processes) where we could contribute with our broad know-how and technical skills.
CONTACT
In case you were interested in our collaboration, please feel free to contact us at:
Principal Investigator: [email protected] Tech Transfer: [email protected] Tel: +34 91 837 31 12 Ext: 4348/4349 Fax: +34 91 536 04 32 c/ Ramiro de Maeztu, 9. 28040 Madrid. CIB-CSIC Lab 347
http://www.cib.csic.es/en/grupo.php?idgrupo=61
SSTTRRUUCCTTUURRAALL BBIIOOPPHHYYSSIICCSS OOFF CCEELLLL DDEEAATTHH BBYY NNMMRR –– DDrr.. EEvvaa ddee AAllbbaa
EXECUTIVE SUMMARY
In our group we aim at gaining an in-depth understanding on the molecular bases of programmed cell death (apoptosis) using an approach based on Structural Biology and Biophysics. The dysfunction of apoptotic pathways derives in several pathologies including cancer, autoimmune and neurodegenerative disorders.
The group focuses its research in determining protein 3D
structure by high-resolution nuclear magnetic resonance (NMR) and other biophysical methods together with protein biochemistry, molecular biology and peptide synthesis. The final objective is to study structure (molecular and atomic), dynamics, protein interactions, recognition and oligomerization, of proteins involved in cell death pathways, protein-membrane binding, connections between order/disorder and function in natively unfolded proteins, coupled folding and binding and protein membrane structure, mechanisms and intrinsically disordered proteins in general.
Electrostatic surface of gpW
CURRENT RESEARCH THEMES OF THE GROUP
Specific current projects currently developed by the group are:
♦ Biophysics of intrinsically disordered proteins. E.g., members of the BCL-2 family of apoptosis regulator proteins.
♦ Structure, dynamics and oligomerization of modular proteins of the Death Domain superfamily.
SPECIFIC SKILLS
Our group has strong expertise in all aspects of multidimensional homo- and hetero-nuclear solution NMR experiments using magnetic field NMR spectrometers (600 MHz spectrometers), with a solid background in several NMR fields:
♦ Pulse sequence programming and implementation
♦ NMR data acquisition, processing and analysis
♦ Protein structure calculation
♦ Refinement and analysis of protein dynamics from magnetic relaxation measurements
Tentative structural model of Harakiri´s operating mode
Moreover, we have a strong expertise for combining this NMR data with other techniques such as AFM, CD, fluorescence microscopy, ELISA, etc. to provide deeper analysis.
GENERAL AND COMPLEMENTARY SKILLS
♦ Molecular biology standard techniques
♦ Protein and peptide production (CIB Service)
SIGNIFICANT EQUIPMENT
♦ 600 MHz Spectrometer
OUR TEAM
Dra. Eva de Alba is an independent researcher at CIB since 2007 and was staff scientist at the National Institutes of Health (USA) from 2002 to 2007. Besides about 25 articles (707 cites in SCOPUS), she has written several book chapters and several reviews in NMR methodology, as well as being awarded in 2006 with a Marie Curie International Reintegration Grant to move from the USA to CIB-CSIC
(Spain).
The group is also formed by four post-doctoral scientists with multidisciplinary training.
Click here to see selected publications.
COLLABORATIONS & PARTNERSHIPS
Prodestech. Prodestech is a scientific consortium devised to make specific advancements towards the goals of Protein Design and Engineering technologies in a synergistic way wherein the group participates actively. The project is funded since 2009 by Spanish Ministry of Science & Technology through the Consolider Programme.
We are open to make available our broad knowledge and multidisciplinary technical skills on proteins and NMR in any type of projects (better if related with our disease fields) with the aim of going forward in the global knowledge at this field.
CONTACT
The group is based in the CIB, belonging to CSIC. In case you were interested in our collaboration, please feel free
to contact us at:
Principal Investigator: [email protected]
Tech Transfer: [email protected]
Tel: +34 91 837 31 12 Ext: 4390
Fax: +34 91 536 04 32
c/ Ramiro de Maeztu, 9. 28040 Madrid. CIB-CSIC Lab B05
http://www.cib.csic.es/en/grupo.php?idgrupo=69
TTIISSSSUUEE EENNGGIINNEEEERRIINNGG GGRROOUUPP.. PPrrooff.. JJoosséé LLuuiiss LLóóppeezz LLaaccoommbbaa
EXECUTIVE SUMMARY
The Tissue Engineering Group (TEG) of UCM develops different clinical applications for recombinant human bone morphogenetic proteins (rhBMPs) in tissue engineering since 1996. In order to reach main goals the group acquired multidisciplinary skills in the areas of cell biology, materials and biological factors.
CURRENT RESEARCH THEMES OF THE GROUP
Our going research focus on the field of tissue engineering employing production and manipulation of biologically active factors developed in cell lines as well as develop therapeutic applications based on new materials.
Target therapeutic areas are:
♦ Bone Tissue
♦ Articular Cartilage
♦ Nervous System
Three-dimensional studies, cell visualization and location in scaffolds
SPECIFIC SKILLS
The technological expertise of the Dr. Lacomba´s group includes protein purification and molecular biology, cell culture, cells assays and labeling, microarrays and quantitative PCR, microscopy (normal, surface, fluorescence, confocal and interferometric), SEM, ESEM,
μCT techniques, and development and manufacturing of biopolymeric scaffoldings.
On the other hand we are used to develop fieldwork performing animal surgery especially in ovine, in order to apply developed therapies in bone regeneration.
Regeneration of the bone treated with our carrier activated with
rgBMP-2 in the control sheep.
GENERAL AND COMPLEMENTARY SKILLS
Cell and molecular biology general skills are regularly applied in our lab under strict quality standards.
OUR TEAM
The group is headed by Dr. José Luis López-Lacomba and Dr. José Vicente Sanz Casado. Dr. Lacomba is Full Professor at the UCM since 1989, after work at University of Granda (Ph.D.), John Hopkins University (Mariland, US), and Biozentrum der Universität (Basel, Switzerland). He has published around 25 research articles (cited 521 times in the
Thompson ISI) and is the investigator of the patents developed by Noricum.
Click here to see selected publications
COLLABORATIONS & PARTNERSHIPS
Since its creation the group has been supported by private companies –such as Centro de Oseointegración M-C, and Osfarma– and foundations –MICINN, FIB MMA– until, as
consequence of the application of the research into a patent, it was created a spin-off named Noricum in order to exploit clinical applications.
Prodestech. Prodestech is a scientific consortium devised to make specific advancements towards the goals of Protein Design and Engineering technologies in a synergistic way wherein the group participates actively. The project is funded since 2009 by Spanish Ministry of Science & Technology through the Consolider Programme.
We are open to make available our broad knowledge and multidisciplinary technical skills on proteins and specifically on protein refolding in any type of projects with the aim of going forward in the global knowledge at this field.
We are looking for joining to consortiums or offer research or consultancy services interested in joining to projects where we could contribute with our know-how and technical skills
CONTACT
In case you were interested in our collaboration, please feel free to contact us at:
Principal Investigator: [email protected]
Tech Transfer: [email protected]
Tel: +34 91 394 32 88
Instituto de Estudios Biofuncionales. Pº Juan XXIII, 1. Basement Level. 28040, Madrid
http://portal.ucm.es/web/ingenieria-de-tejidos/inicio
STRUCTURAL BIOLOGY OF MACROMOLECULAR ASSEMBLIES. Prof. Antonio Romero & Prof. Guillermo Gallego
EXECUTIVE SUMMARY
Our research group aims to unveil the structural basis of human diseases, covering from basic research in protein function to drug development. For this we use X-ray crystallography and electron microscopy, combined with other biophysical and biochemical techniques. Our investigations focus on bacterial infection and resistance, as well as on repair, transcription and growth factors associated
with tumorigenic and neurological processes.
CURRENT RESEARCH LINES
♦ Proteins involved in bacterial antibiotic resistance
♦ Structural studies on the bacterial secretion machinery
♦ Structure of DNA repair factors
♦ Structural basis of transcription
♦ Discovery of lead-compounds to develop novel drugs that inhibit the FGF signaling pathway and other proteins
♦ Formation of the cell nucleus
SPECIFIC SKILLS
♦ X-ray crystallography
♦ Electron microscopy
♦ Isolation of macromolecular complexes
♦ Structure-based drug design
OXA24 by X-ray New drug against FGF RNA Pol III by EM
GENERAL & COMPLEMENTARY SKILLS
THE GROUP
Prof. Antonio Romero carried out his Ph.D. at the Universidad Complutense de Madrid and postdocs at the University of Rennes (France) and the Max-Planck Institute of Biochemistry in Munich (Germany). Specific research interests focus on the structural biology of proteins involved in antibiotic resistance.
Prof. Guillermo Giménez-Gallego was part of the team that isolated and described the fibroblast growth factor (FGF) at
Merck. He showed that FGFs are important pro-inflammatory proteins and led the discovery of inhibitors that are now in clinical trials against different inflammatory diseases. He also discovered Iberotoxin and Charibdotoxin, two essential tools in electrophysiology. His publications have been cited above 6000 times. He was director of CIB for 8 years.
Dr. Carlos Fernández-Tornero obtained his PhD at the Universidad Autónoma de Madrid. After a postdoc at EMBL-Grenoble in crystallography and EM of transcription complexes, he became staff scientist at EMBL-Heidelberg. Since 2010 he leads a research team at CIB-CSIC.
The group is further constituted by 5 postdocs in multidisciplinary fields, 2 PhD students and 3 technicians.
Click here to see selected publications
EQUIPMENT & FACILITIES
♦ X-ray diffractometer
♦ Crystallization robots
♦ Molecular biology equipment
♦ Protein purification equipment
COLLABORATIONS & PARTNERSHIPS
Prodestech. Prodestech is a scientific consortium devised to make specific advances in the fields of protein design and engineering technologies, where the group participates actively. The project is funded since 2009 by the Spanish Ministry of Science & Technology through the Consolider Programme.
The group has established national and international
collaborations with experts in different fields, such as microbiology, cell biology, yeast genetics, native mass spectrometry, bioinformatics, crystal growth, and clinical applications.
Industrial collaborations have been developed during the years with several pharmaceutical companies, including Rovi, CEMTRO, IPF-Pharmaceuticals, PharmaMar and NeuroPharma.
CONTACT
We aim to adapt ourselves to the new changing world betting for the excellence on basic research, including collaborations with other research groups and close contacts with the industrial area.
We are open to share our broad knowledge and multidisciplinary technical skills in structural biology, in order to deepen our knowledge on the molecular basis of human diseases. We seek to participate in research consortiums where our expertise can contribute to achieve specific goals.
Scientific contact: [email protected],
[email protected], [email protected]
Tech transfer: [email protected]
Tel: +34 91 837 31 12 Ext: 4244/4378/4327
Fax: +34 91 536 04 32
Nano-crystallization robot
X-ray generator Visualization system
♦ Molecular biology
♦ Bioinformatics
♦ Protein biochemistry
♦ Other biophysical methods
Centro de Investigaciones Biológicas - CSICRamiro de Maeztu 9, 28040 Madrid (Spain)
http://www.cib.csic.es/en/grupo.php?idgrupo=40
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