Micro- and Nano-Techniques for Analysis and Characterization of Proteins PROGRAM Madrid, 22-23 July 2019 MONDAY, 22 JULY 8:30-9:00 REGISTRATION Lobby 9:00-9:25 WELCOME FROM CSIC-CNRS/ INTRODUCTION TO THE WORKSHOP Piano Conference Room ELENA DOMÍNGUEZ.Vice-president of International Affair of CSIC. LAURA MOLINARI Coopération Bilatérale Derci-CNR. MÓNICA MARTÍN-LANUZA Coordinator of International Cooperation and Resources of CSIC. JOSÉ CARLOS DIEZ-MASA Institute or Organic Chemistry (IQOG-CSIC). 9:30-10:30 SESION 1 Piano Conference Room Chair: JOËLLE VINH and GERMAN RIVAS 9:30-9:50 MYRIAM TAVERNA Team “Proteins and Nanotechnology in Analytical Science” and extreme pre-concentration of disease biomarkers C. CROSNIER DE LASSICHÈRE, T. TRAN, M. ARAYAS-FARIAS, T.D. MAI, M. TAVERNA. Institut Galien Paris-Sud, Université Paris-Sud, Châtenay-Malabry, France. 9:50-10:10 FERNANDO CORRALES Proteomics: translating genes into cellular functions to understand biology and disease ALBERTO PARADELA 1 , VERÓNICA AMBAO 2 , IGNACIO GRANERO 1 , LAURA GUERRERO 1 , FERNANDO J. CORRALES 1 . 1 Centro Nacional de Biotecnología, CSIC, CIBEREHD, Madrid, Spain. 2 Centro de Investigaciones Endocrinológicas CONICET, Buenos Aires, Argentina. 10:10-10:30 STÉPHANIE DESCROIX Microfluidic development for bioanalysis and clinics STÉPHANIE DESCROIX 1,2,3 1 Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, CNRS UMR168, 75005 Paris, France. 2 Sorbonne niversités, UPMC Univ Paris 06, 75005 Paris, France. 3 Institut Pierre- Gilles de Gennes, 75005 Paris, France. 10:30-12:00 COFFEE PAUSE AND POSTER VISIT Billiards Basement 12:05-12:45 SESSION 2 Piano Conference Room Chair: MYRIAM TAVERNA and JOSÉ MARÍA VALPUESTA 12:05-12.25 JOËLLE VINH Comprehensive glycosylation mapping of a highly glycosylated hormone using high resolution mass spectrometry of glycoproteoforms and glycopeptides combined to matrix-assisted glycan population analysis NICOLAS ESKENAZI, CHIARA GIANGRANDE, OPHELIA DJIMATEY, JOËLLE VINH. Biological Mass Spectrometry and Proteomics (SMBP),USR 3149 CNRS - ESPCI Paris, PSL University, Paris France. 12:25-12:45 GERMAN RIVAS Biochemical reconstruction from the bottom- up of minimal bacterial division machineries in cytomimetic environments GERMÁN RIVAS. CIB-CSIC, Madrid, Spain. 12:50-14:15 FLASH COMMUNICATIONS 1 Piano Conference Room Chair: ANNE VARENNE and MERCEDES DE FRUTOS 12:50-13:00 LAURA TRAPIELLA Optical imaging nanoprobes based on clickable- zwitterionic copolymer capped quantum dots for in vivo targeting: application to α,β-integrins for cancer diagnosis LAURA TRAPIELLA-ALFONSO 1, THOMAS PONS 2 , BICH-THUY DOAN 1, 3 , FANNY D’ORLYÉ 1 , NICOLAS LEQUEUX 2 , AND ANNE VARENNE 1 1 i-CLeHS CNRS 2027, Chimie ParisTech, PSL Research University, Paris, France. 2 LPEM CNRS 8213, ESPCI, PSL Research University, Paris, France. 3 Plataforme Imageries du Vivant, Faculté de Pharmacie, Université Paris Descartes. 13:00-13:10 LAURA GÓMEZ RUIZ Therapeutic immunoglobulins analyzed by capillary electrophoresis. LAURA GOMEZ-RUIZ, ÁNGEL PUERTA, JOSÉ CARLOS DIEZ-MASA, MERCEDES DE FRUTOS. Institute of Organic Chemistry (IQOG-CSIC), Madrid, Spain. 13:00-13:10 SMANTHA BOURG Development of aptamer-based microfluidic systems for the detection of protein biomarkers of cancer SAMANTHA BOURG, FANNY D’ORLYÉ, SOPHIE GRIVEAU, ALBERTO FRACASSI DA SILVA, ANNE VARENNE, FETHI BEDIOUI. Chimie Paris Tech, PSL Université Paris, CNRS 2027, Institute of Chemistry for Life and Health Sciences, (i-CLeHS). 13:10-13:20 MARTA JAÉN Analysis of protein alterations after treatment of KM12SM cells with an Anti-cadherin 17 monoclonal antibodies using global proteomic analysis MARTA JAÉN 1 , RUBÉN A. BARTOLOMÉ 1 , VIVIAN DE LOS RÍOS 1 , MARI CARMEN GARCÍA 2 , LAURA PINTADO 1 , ÁNGELA MARTÍN-REGALADO 1 , JUAN IGNACIO IMBAUD 2 AND J. IGNACIO CASAL 1 . 1 Department of Molecular Biomedicine. Centro de Investigaciones Biológicas (CSIC). 28040, Madrid, Spain. 2 Protein Alternatives SL. 28760, Tres Cantos, Madrid, Spain. 13:20-13:30 MERIEM DADOUCH In-line middle-up analysis of monoclonal antibodies by capillary electrophoresis MERIEM DADOUCH 1 , YOANN LADNER 2 , CLAUDIA BICH 3 , MARION LARROQUE 4 , CHRISTIAN LARROQUE 5 , JACQUES MOREL 6 , PIERRE- ANTOINE BONNET 7 , CATHERINE PERRIN 8 . 1 Institut des Biomolécules Max Mousseron (IBMM), UMR 5247-CNRS- UM-ENSCM, Université de Montpellier, Montpellier, France. 2 Institut des Biomolécules Max Mousseron (IBMM), UMR 5247-CNRS-UM- ENSCM, Université de Montpellier, Montpellier, France. 3 Institut des Biomolécules Max Mousseron (IBMM), UMR 5247-CNRS- UM-ENSCM, Université de Montpellier, Montpellier, France. 4 Institut de Recherche en Cancérologie de Montpellier (IRCM), INSERM U1194, Montpellier, France. 5 Institut de Recherche en Cancérologie de Montpellier (IRCM), INSERM U1194, Montpellier, France. 6 Département de Rhumatologie, Université de Montpellier, Hôpital Lapeyronie, 34295, Montpellier cedex 5, France. 7 Institut des Biomolécules Max Mousseron (IBMM), UMR 5247-CNRS-UM- ENSCM, Université de Montpellier, Montpellier, France. 8 Institut des Biomolécules Max Mousseron (IBMM), UMR 5247-CNRS-UM- ENSCM, Université de Montpellier, Montpellier, France. 13:30-13:40 CRISTINA SANTIAGO PERTUSA Development of 2D array of microfluidic wells to induce cell aggregation CRISTINA SANTIAGO PERTUSA, ITZIAR GONZÁLEZ GÓMEZ. Institute for Physical and Information Technologies, CSIC, Madrid, Spain. 13:40-13:50 BENOîT CHOVELON Mirror-image aptamer kissing complex for arginine-vasopressin sensing BENOÎT CHOVELON 1, 2, EMMANUELLE FIORE 1 , PATRICE FAURE 2 ,3, ERIC PEYRIN 1 , CORINNE RAVELET 1 . 1 DPM UMR 5063, F-38041 Grenoble; CNRS, DPM UMR 5063, University Grenoble Alpes, F-38041 Grenoble, France. 2 Département de Biochimie, Toxicologie et Pharmacologie, CHU de Grenoble site Nord - Institut de biologie et de pathologie, F-38041 Grenoble, France. 3 Laboratory of Hypoxy Physiopathology Study Inserm U1042, University Grenoble Alpes, 38700 La Tronche, France. 14:00-15:40 LUNCH TIME Dining Room 15:45-17:05 SESSION 5 Piano Conference Room Chair: ERIC PEYRIN AND MIGUEL HERREO 15:45-16:05 IGNACIO CASAL Unraveling the “dark” proteome in cancer metastasis IGNACIO CASAL, RUBÉN A. BARTOLOMÉ, MARTA JAÉN, LAURA PINTADO, ÁNGELA MARTIN-REGALADO. Department of Molecular Biomedicine. Centro de Investigaciones Biológicas (CSIC). 28040 Madrid. Spain. 16:05-16:25 CATHERINE PERRIN Capillary electrophoresis: from separation to nano-enzymatic reactor. A powerful tool for the quality control of therapeutic proteins YOANN LADNER 1 , MERIEM DADOUCH 1 , CLAUDIA BICH 1 , GAËLLE COUSSOT 1 , CHRISTIAN LARROQUE 2 , CATHERINE PERRIN 1 . 1 Institut des Biomolécules Max Mousseron (IBMM), UMR 5247-CNRS- UM-ENSCM, Université de Montpellier, Montpellier, France. 2 Institut de Recherche en Cancérologie de Montpellier (IRCM), INSERM U1194, Montpellier, France. 16:25-16:45 JOSÉ REQUEJO-ISIDRO Lipid-protein interplay. A quantitative view through the microscope lens JOSÉ REQUEJO-ISIDRO. Centro Nacional de Biotecnología (CNB), CSIC, Madrid. Spain 16:45-17:05 FLORENCE GONNET Coupling of different techniques with mass spectrometry to probe biomolecular interactions FLORENCE GONNET, ANASTASIIA HALUSKINA, ANNA MŁYNARCZYK WILLIAM BUCHMANN, RÉGIS DANIEL. Univ Evry, CNRS, Université Paris-Saclay, Laboratoire Analyse et Modélisation pour la Biologie et l’Environnement (LAMBE), Evry, France. 17:10 FAREWELL FROM CSIC-CNRS Organized by:
Transcript
Micro- and Nano-Techniques for Analysis and Characterization of Proteins
PROGRAMMadrid, 22-23 July 2019
MONDAY, 22 JULY8:30-9:00 REGISTRATIONLobby
9:00-9:25 WELCOME FROM CSIC-CNRS/ INTRODUCTION TO THE WORKSHOPPiano Conference RoomELENA DOMÍNGUEZ.Vice-president of International Affair of CSIC.LAURA MOLINARI Coopération Bilatérale Derci-CNR.MÓNICA MARTÍN-LANUZA Coordinator of International Cooperation and Resources of CSIC.JOSÉ CARLOS DIEZ-MASA Institute or Organic Chemistry (IQOG-CSIC).
9:30-10:30 SESION 1Piano Conference RoomChair: JOËLLE VINH and GERMAN RIVAS
9:30-9:50 MYRIAM TAVERNATeam “Proteins and Nanotechnology in Analytical Science” and extreme pre-concentration of disease biomarkersC. CROSNIER DE LASSICHÈRE, T. TRAN, M. ARAYAS-FARIAS, T.D. MAI, M. TAVERNA.Institut Galien Paris-Sud, Université Paris-Sud, Châtenay-Malabry, France.
9:50-10:10 FERNANDO CORRALESProteomics: translating genes into cellular functions to understand biology and diseaseALBERTO PARADELA1, VERÓNICA AMBAO2, IGNACIO GRANERO1, LAURA GUERRERO1, FERNANDO J. CORRALES1.1Centro Nacional de Biotecnología, CSIC, CIBEREHD, Madrid, Spain.2Centro de Investigaciones Endocrinológicas CONICET, Buenos Aires, Argentina.
10:10-10:30 STÉPHANIE DESCROIXMicrofl uidic development for bioanalysis and clinicsSTÉPHANIE DESCROIX1,2,3 1Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, CNRS UMR168, 75005 Paris, France. 2Sorbonne niversités, UPMC Univ Paris 06, 75005 Paris, France. 3Institut Pierre-Gilles de Gennes, 75005 Paris, France.
10:30-12:00 COFFEE PAUSE AND POSTER VISITBilliards Basement
12:05-12:45 SESSION 2Piano Conference RoomChair: MYRIAM TAVERNA and JOSÉ MARÍA VALPUESTA
12:05-12.25 JOËLLE VINHComprehensive glycosylation mapping of a highly glycosylated hormone using high resolution mass spectrometry of glycoproteoforms and glycopeptides combined to matrix-assisted glycan population analysisNICOLAS ESKENAZI, CHIARA GIANGRANDE, OPHELIA DJIMATEY, JOËLLE VINH.Biological Mass Spectrometry and Proteomics (SMBP),USR 3149 CNRS - ESPCI Paris, PSL University, Paris France.
12:25-12:45 GERMAN RIVAS Biochemical reconstruction from the bottom-up of minimal bacterial division machineries in cytomimetic environmentsGERMÁN RIVAS. CIB-CSIC, Madrid, Spain.
12:50-14:15 FLASH COMMUNICATIONS 1Piano Conference RoomChair: ANNE VARENNE and MERCEDES DE FRUTOS
12:50-13:00 LAURA TRAPIELLAOptical imaging nanoprobes based on clickable-zwitterionic copolymer capped quantum dots for in vivo targeting: application to α,β-integrins for cancer diagnosisLAURA TRAPIELLA-ALFONSO1, THOMAS PONS2, BICH-THUY DOAN1, 3, FANNY D’ORLYÉ1, NICOLAS LEQUEUX2, AND ANNE VARENNE1
1i-CLeHS CNRS 2027, Chimie ParisTech, PSL Research University, Paris, France. 2LPEM CNRS 8213, ESPCI, PSL Research University, Paris, France.3Plataforme Imageries du Vivant, Faculté de Pharmacie, Université Paris Descartes.
13:00-13:10 LAURA GÓMEZ RUIZTherapeutic immunoglobulins analyzed by capillary electrophoresis.LAURA GOMEZ-RUIZ, ÁNGEL PUERTA, JOSÉ CARLOS DIEZ-MASA, MERCEDES DE FRUTOS. Institute of Organic Chemistry (IQOG-CSIC), Madrid, Spain.
13:00-13:10 SMANTHA BOURGDevelopment of aptamer-based microfl uidic systems for the detection of protein biomarkers of cancerSAMANTHA BOURG, FANNY D’ORLYÉ, SOPHIE GRIVEAU, ALBERTO FRACASSI DA SILVA, ANNE VARENNE, FETHI BEDIOUI.Chimie Paris Tech, PSL Université Paris, CNRS 2027, Institute of Chemistry for Life and Health Sciences, (i-CLeHS).
13:10-13:20 MARTA JAÉN Analysis of protein alterations after treatment of KM12SM cells with an Anti-cadherin 17 monoclonal antibodies using global proteomic analysisMARTA JAÉN1, RUBÉN A. BARTOLOMÉ1, VIVIAN DE LOS RÍOS1, MARI CARMEN GARCÍA2, LAURA PINTADO1, ÁNGELA MARTÍN-REGALADO1, JUAN IGNACIO IMBAUD2 AND J. IGNACIO CASAL1.1Department of Molecular Biomedicine. Centro de Investigaciones Biológicas (CSIC). 28040, Madrid, Spain. 2Protein Alternatives SL. 28760, Tres Cantos, Madrid, Spain.
13:20-13:30 MERIEM DADOUCHIn-line middle-up analysis of monoclonal antibodies by capillary electrophoresisMERIEM DADOUCH1, YOANN LADNER2, CLAUDIA BICH3, MARION LARROQUE4, CHRISTIAN LARROQUE5, JACQUES MOREL6, PIERRE-ANTOINE BONNET7, CATHERINE PERRIN8.1Institut des Biomolécules Max Mousseron (IBMM), UMR 5247-CNRS-UM-ENSCM, Université de Montpellier, Montpellier, France. 2Institut des Biomolécules Max Mousseron (IBMM), UMR 5247-CNRS-UM-ENSCM, Université de Montpellier, Montpellier, France. 3 I n s t i t u t des Biomolécules Max Mousseron (IBMM), UMR 5247-CNRS-UM-ENSCM, Université de Montpellier, Montpellier, France. 4Institut de Recherche en Cancérologie de Montpellier (IRCM), INSERM U1194, Montpellier, France. 5Institut de Recherche en Cancérologie de Montpellier (IRCM), INSERM U1194, Montpellier, France. 6Département de Rhumatologie, Université de Montpellier, Hôpital Lapeyronie, 34295, Montpellier cedex 5, France. 7Institut des Biomolécules Max Mousseron (IBMM), UMR 5247-CNRS-UM-ENSCM, Université de Montpellier, Montpellier, France. 8 I n s t i t u t des Biomolécules Max Mousseron (IBMM), UMR 5247-CNRS-UM-ENSCM, Université de Montpellier, Montpellier, France.
13:30-13:40 CRISTINA SANTIAGO PERTUSADevelopment of 2D array of microfl uidic wells to induce cell aggregationCRISTINA SANTIAGO PERTUSA, ITZIAR GONZÁLEZ GÓMEZ.Institute for Physical and Information Technologies, CSIC, Madrid, Spain.
13:40-13:50 BENOîT CHOVELONMirror-image aptamer kissing complex for arginine-vasopressin sensingBENOÎT CHOVELON1, 2, EMMANUELLE FIORE1, PATRICE FAURE2 ,3, ERIC PEYRIN1, CORINNE RAVELET1.1DPM UMR 5063, F-38041 Grenoble; CNRS, DPM UMR 5063, University Grenoble Alpes, F-38041 Grenoble, France. 2Département de Biochimie, Toxicologie et Pharmacologie, CHU de Grenoble site Nord - Institut de biologie et de pathologie, F-38041 Grenoble, France. 3Laboratory of Hypoxy Physiopathology Study Inserm U1042, University Grenoble Alpes, 38700 La Tronche, France.
14:00-15:40 LUNCH TIMEDining Room
15:45-17:05 SESSION 5 Piano Conference RoomChair: ERIC PEYRIN AND MIGUEL HERREO
15:45-16:05 IGNACIO CASALUnraveling the “dark” proteome in cancer metastasisIGNACIO CASAL, RUBÉN A. BARTOLOMÉ, MARTA JAÉN, LAURA PINTADO, ÁNGELA MARTIN-REGALADO.Department of Molecular Biomedicine. Centro de Investigaciones Biológicas (CSIC). 28040 Madrid. Spain.
16:05-16:25 CATHERINE PERRINCapillary electrophoresis: from separation to nano-enzymatic reactor. A powerful tool for the quality control of therapeutic proteinsYOANN LADNER1, MERIEM DADOUCH1, CLAUDIA BICH1, GAËLLE COUSSOT1, CHRISTIAN LARROQUE2, CATHERINE PERRIN1.1Institut des Biomolécules Max Mousseron (IBMM), UMR 5247-CNRS-UM-ENSCM, Université de Montpellier, Montpellier, France. 2Institut de Recherche en Cancérologie de Montpellier (IRCM), INSERM U1194, Montpellier, France.
16:25-16:45 JOSÉ REQUEJO-ISIDROLipid-protein interplay. A quantitative view through the microscope lensJOSÉ REQUEJO-ISIDRO.Centro Nacional de Biotecnología (CNB), CSIC, Madrid. Spain
16:45-17:05 FLORENCE GONNETCoupling of different techniques with mass spectrometry to probe biomolecular interactionsFLORENCE GONNET, ANASTASIIA HALUSKINA, ANNA MŁYNARCZYK WILLIAM BUCHMANN, RÉGIS DANIEL.Univ Evry, CNRS, Université Paris-Saclay, Laboratoire Analyse et Modélisation pour la Biologie et l’Environnement (LAMBE), Evry, France.
17:10 FAREWELL FROM CSIC-CNRS
Organized by:
13:10-13:20 LORENA CARMONAUnderstanding liver regeneration. Proteomic and phosphoproteomic analysis.LORENA CARMONA, MATÍAS ÁVILA, ROSANA NAVAJAS, ANTONIO RAMOS, SERGIO CIORDIA, FERNANDO CORRALES. Centro Nacional de Biotecnología, CSIC, CIBEREHD, Madrid, Spain.
13:20-13:30 LUIS HERNÁNDEZ ALVAREZCell monolayer manipulation with low Intensity ultrasoundsLUIS HERNÁNDEZ ÁLVAREZ1, ICÍAR GONZÁLEZ GÓMEZ1, JULIE EARL2.1Institute for Physical and Information Technologies, CSIC, Madrid, Spain. 2Oncology Department, Ramón y Cajal Hospital, Madrid, Spain.
16:30-17:50 SESSION 3Piano Conference RoomChair: STÉPHANIE DESCROIX and ITZIAR GONZÁLEZ
16:30-16:50 CLAIRE SMADJADeep view into analytical strategies to address the quality control of therapeutic monoclonal antibodiesMINH THANH LE1, E. JACCOULET2, T. TRAN1, M. TAVERNA1, C. SMADJA1.1Institut Galien Paris-Sud, Châtenay-Malabry, France. 2Hôpital européen Georges Pompidou, Paris, France.
16:50-17:10 ANA CUERVO The structures of T7 bateriophage portal and tail machinery unveil the mechanism for retention and ejection of viral DNAANA CUERVO1†, MONTSERRAT FÀBREGA-FERRER2,3†, CRISTINA MACHÓN 2,3, JOSÉ JAVIER CONESA 1,4, FRANCISCO JAVIER FERNÁNDEZ5, ROSA PÉREZ-LUQUE2,3, MAR PÉREZ-RUIZ1, JOAN POUS2, MARÍA CRISTINA VEGA, JOSÉ LOPEZ CARRASCOSA1, MIQUELL COLL2,3.1Centro Nacional de Biotecnología, CSIC, Darwin 3, Madrid, 28049, Spain. 2Institute for Biomedical Research (IRB Barcelona), Baldiri Reixac 10, Barcelona, 08028, Spain. 3Institut de Biologia Molecular de Barcelona (IBMB-CSIC), Baldiri Reixac 10, Barcelona. 4Present address: ALBA Synchrotron Light Source, 08290 Barcelona, Spain. 08028, Spain. 5Centro de Investigaciones Biológicas (CIB-CSIC), Ramiro de Maeztu 9, Madrid, 28040, Spain.
17:10-17:30 MARIE LECOEUR Interest of capillary isoelectric focusing for protein characterization and quantitationMARIE LECOEUR1, ANNE VARENNE2, PIERRE GAREIL3, CLAUDE VACCHER4, JEAN-PAUL BONTE5, JEAN-FRANÇOIS GOOSSENS6, CATHERINE FOULON7.1Univ Lille, Faculté de Pharmacie, Groupe de Recherche sur les Formes Injectables et Technologies Associées - EA GRITA 7365, Lille, France. 2Chimie ParisTech PSL, Ecole Nationale Supérieure de Chimie, Unité de Technologies Chimiques et Biologiques pour la Santé, UMR 8258 CNRS, INSERM 1022, Paris, France. 3 ,4 ,5Retired professors. 6Univ Lille, Faculté de Pharmacie, Groupe de Recherche sur les Formes Injectables et Technologies Associées - EA GRITA 7365, Lille, France. 7Univ Lille, Faculté de Pharmacie, Groupe de Recherche sur les Formes Injectables et Technologies Associées - EA GRITA 7365, Lille, France.
17:30-17:50 GERARDO ÁLVAREZHigh-resolution tandem mass spectrometry for multi-allergen analysis of fi ning-related egg and milk proteins in red wines.GERARDO ÁLVAREZ-RIVERA1, JESSY PAVÓN2, KAREM HENRIQUEZ2, 3, MARIO ARANDA2, 3, ALEJANDRO CIFUENTES1, MIGUEL HERRERO1
1Laboratory of Foodomics, Institute of Food Science ResearchCIAL, CSIC, Nicolás Cabrera 9, 28049 Madrid, Spain. 2Laboratory of Advanced Research on Foods and Drugs, Department of Food Science and Technology, Faculty of Pharmacy, University of Concepcion, Chile. Barrio Universitario s/n, Concepcion, Chile. 3Center for Biotechnology, University of Concepcion, Barrio Universitario s/n, Concepcion, Chile.
18:00-18:30 CULTURAL TALKPiano Conference Room CAMINO ÁLVAREZThe Residencia de Estudiantes: An Epoch in the Contemporary Culture of SpainFundación Residencia de Estudiantes.
18:30 FREE TIME
20:30 DINNER AT THE EMPERATRIZ HOTELCalle López de Hoyos, 4 (Close to Paseo de la Castellana)
TUESDAY, 23 JULY9:00-10:20 SESION 4Piano Conference RoomChair: CLAIRE SMADJA and FERNANDO CORRALES
9:00-10:20 ERIC PEYRINSelection of Tau protein-binding DNA aptamers by using the capillary electrophoresis partitioning techniqueERIC PEYRIN, EMMANUELLE FIORE, CORINNE RAVELET.Univ. Grenoble Alpes, DPM UMR 5063, CNRS, F-38041 Grenoble, France.
9:20-9:40 MERCEDES DE FRUTOSRelevant glycoproteins in health and food: cooperation of liquid chromatography and capillary electrophoresis for analysis of their isoformsMERCEDES DE FRUTOS, ANGEL PUERTA, SERGIO LOPEZ-DUQUE, LAURA GOMEZ-RUIZ, RAQUEL SAEZ-BROX, JOSE CARLOS DIEZ-MASA. Institute of Organic Chemistry (IQOG-CSIC), Madrid, Spain.
9:40-10:00 FANNY D’ORLYÉFrom online capillary isoelectric focusing-electrospray ionization mass spectrometry towards. Integrated paper-based electrophoretic device for protein characterizationMENEL BEN FREJ, FANNY D’ORLYÉ, ANNE VARENNE.i-CLeHS CNRS 2027, Chimie ParisTech, PSL Research University, Paris, France.
10:00-10:20 JOSE MARÍA VALPUESTAStructural and functional analysis of the role of the chaperonin CCT in mTOR complex assemblyJORGE CUÉLLAR1, W. GRANT LUDLAM2, NICOLE C. TENSMEYER2, TAKUMA AOBA2, MADHURA DHAVALE2, CÉSAR SANTIAGO1, M. TERESA BUENO-CARRASCO1, MICHAEL J. MANN2, REBECCA L. PLIMPTON2, AMAN MAKAJU3, SARAH FRANKLIN3, BARRY M. WILLARDSON2*, JOSÉ M. VALPUESTA1*
1Centro Nacional de Biotecnología, Campus de la Universidad Autónoma de Madrid, 28049 Madrid, Spain. 2Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602. USA. 3Department of Internal Medicine, Nora Eccles Harrison Cardiovascular Research and Training Institute, University of Utah, Salt Lake City, UT 84112. USA.
10:20-11:40 COFFEE PAUSE AND POSTER VISITBilliards Basement
11:45-12:25 SESSION 5 Piano Conference RoomChair: CATHERINE PERRIN and IGNACIO CASALS
11:45-12:05 ANNE VARENNEAnalytical devices for biomedical applicationsFANNY D’ORLYÉ, LAURA TRAPIELLA, SOPHIE GRIVEAU, FETHI BEDIOUI, BICH-THUY DOAN, ANNE VARENNE.i-CLeHS CNRS 2027, Chimie ParisTech, PSL Research University, Paris, France.
12:05-12:25 ITZIAR GONZÁLEZUltrasounds and microfl uidics for cell sorting and separation: development of lab-on-chip devices of low-cost fabricationITZIAR GONZÁLEZ.Institute for Physical and Information Technologies, CSIC, Madrid, Spain.
12:30-13:50 FLASH COMMUNICATIONS 2 Piano Conference RoomChair: ANNE VARENNE and MERCEDES DE FRUTOS
12:30-12:40 ÁNGEL DE LA PUERTAInfl uence of the purifi cation process on the features of the target glycoproteinÁNGEL PUERTA1, DIANA NAVARRO-CALDERON1, ANDRAS ACS2,3, NOEMI FARINA-GOMEZ1, EDUARDO ALBERS-ACOSTA4, CARLOS OLIVIER4, LASZLO DRAHOS2, JOSÉ CARLOS DIEZ-MASA1, KAROLY VEKEY2,5, MERCEDES DE FRUTOS1.1Institute of Organic Chemistry (IQOG-CSIC), Madrid, Spain. 2MS Proteomics Research Group, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary. 3Semmelweis University, Budapest, Hungary. 4Department of Urology, Hospital La Princesa, Madrid, Spain. 5Szent Istvan University, Ybl Miklos Faculty of Architecture and Civil Engineering, Budapest, Hungary.
12:40-12:50 THAN DUC MAIPurpose-made instrumentation: a versatile and adaptive tool for diagnostical applicationsTHANH DUC MAI1, CLAIRE SMADJA1, PETER C HAUSER2, STÉPHANIE DESCROIX2, MYRIAM TAVERNA1.1Institut Galien Paris Sud, UMR 8612, Protein and Nanotechnology in Analytical Science (PNAS), CNRS, Univ. Paris-Sud, Univ. Paris-Saclay, 5 rue Jean Baptiste Clément, 92290 Châtenay-Malabry, France. 2University of Basel, Department of Chemistry, Spitalstrasse 51, 4056 Basel, Switzerland. 3Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, CNRS UMR168, 75005 Paris, France.
12:50-13:00 SERGIO CUELLA RUIZ Characterization and analysis of a Lab-On-Chip device of polymeric base for sorting of particles or cells using ultrasoundsSERGIO CUÉLLAR RUIZ, ITZIAR GONZÁLEZ. Institute for Physical and Information Technologies, CSIC, Madrid, Spain
