Sartobind® Ion ExchangeMembrane Adsorber capsules, 4 mm bed height

Sartobind Capsules for Economic Production of Therapeutic Proteins

High speed polishingSartobind SingleSep® capsules are designed to remove charged contaminants from therapeutic proteins at accelerated flow rates by ion exchange membrane chroma-tography. The high throughput is a direct result of negligible mass transfer effects and is made possible by the >3 µm macroporous membrane with 4 mm bed height.

Approved technology In 2001 Campath®* received FDA approval. This monoclonal antibody is polished by application of Sartobind ion exchange Q modules in flow through mode (DNA| endotoxin|leached protein A|virus removal). It was the first time that a Membrane Adsorber has been accepted in the produc-tion of a therapeutic protein. This shows not only Sartorius’ position as the pioneer in adsorptive membrane chromatography but also displays that this technology is proven and tested.

* Campath is a trademark of Genzyme Corporation

Efficient– Higher throughput (g/h) for trace

impurity removal

Economical– Saves capital– No hardware investment & maintenance– No column packing, testing,

regeneration– No re-use validation– Less unspecific binding – higher yield– Less labor – Buffer consumption may be

decreased 95%

Easy to use– Disposable– Simple and fast set up– Handling like a filter capsule

Controlled qualitySartobind SingleSep capsules are designed, developed and manufactured in accord-ance with an ISO 9001 certified Quality Management System. They have passed Plastic Class VI, particles and extractables test according to current United States Pharmacopoeia (USP) and are tested for protein binding capacity prior to release. Each package contains a certificate of quality.

A validation guide is available upon request: Order No.: 85032-536-72.

Ion Exchange Membranes

Sartobind QThe workhorse to remove negatively charged contaminants: DNA, host cell proteins, viruses and endotoxins.

Sartobind SFor the removal of positively charged contaminants such as host cell proteins.

Sartobind STIC®

In addition to conventional anion exchange chemistries, Sartobind STIC PA (primary amine) ligand operates at high salt condi-tions to decrease need for dilution (e.g. of cation exchange pools) when removing negatively charged impurities. With a binding capacity of 1.4 mg/cm2 the salt tolerant adsorber is a specialist for (host cell) protein removal. MVM virus removal at 150 mM NaCl salt result in >4 LRV and DNA removal in >3 LRV.

Capsule designThe Sartobind SingleSep looks like a standard filter capsule except that the adsorbing membrane is reeled on a core to form a cylinder. The flow is from top into the outside channel and then perpen-dicular through the membrane layers to the center of the cylinder and leaves the capsule at the outlet.

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l Fig. 1: Sartobind 30 inch capsule connected to a disposable bag.

IEX gel column

MaterialsLabourConsumables

Materials

Labour

Consumables

Hardware costs  

t

80% less

IEX Membrane Adsorber Capsule

Flow path/ 4 mm bed height

Sartobind capsule

Fig. 2: Cost comparison of columns and Membrane Adsorbers for polishing application

Fig. 3: Schematic flow pattern and cross section of capsulesa) Sartobind pico 0.08 ml b) 10“ capsule 180 ml

a)

b)

Application Area

In a generic downstream process for the production of a monoclonal antibody, the main capturing steps are followed by the polishing step(s).

The later the anion exchanger is applied in the process, the less contaminants have to be removed.

The positively + charged ligand of the Q adsorbers binds negatively _ charged contaminants. Neutral or positively + charged product runs through (see Figure).

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Cell removal|Clarification

Affinity Chromatography

Cation Exchange Chromatography

Anion Exchange Adsorber Polishing Step (flowthrough): Removal of DNA, HCP, Endotoxins

Virus Clearance

Ultra-|Diafiltration

Sartobind usage in monoclonal antibody production:

Endotoxin

DNA Virus

Host cell protein

2 7 12

pH of buffer

MAb

_ _

_

Protein A_ _

_

+

+

pl

Fig. 4: Isoelectric points and charges of MAbs and impurities

Binding capacity on Sartobind ion exchanger membranes

IEX ligand Type of molecule Binding capacity Binding capacity Ref. chemistry per ml per cm2

Q BSA >29 mg >0.8 mg 1

STIC PA BSA 50 mg 1.4 mg 4

Q DNA 5.6 mg 0.16 mg 2

STIC PA DNA 10 mg 0.3 mg 4

Q Adenovirus up to 1.5+1013 up to 8+1011 Viral Particles 3

S Lysozyme >29 mg >0.8 mg 1

1 ml = 36 cm2 IEX membrane1 Sartorius quality control for minimum static

binding capacity2 J. K. Walter, (Boehringer-Ingelheim Pharma) in:

Bioseparation and Bioprocessing, G. Subramanian (ed.) Wiley VCH, 1998, Vol. II p. 447–460

3 Measured with Vivapure AdenoPack 500. Order no.: VS-AVPQ501

4 Measured in high salt buffer, 150 mM NaCl, Sartobind STIC PA manual, Order no.: 85032-536-77

5

The use of conventional chromatography columns for flow through (FT) anion exchange chromatography requires high flow rates. Optimized production columns need a certain diameter and bed height to achieve a large throughput and therefore have a large bed volume. That is the reason why columns are typically oversized.

Due to the cylindrical construction of the adsorber the high flow rate of the membrane is combined with a large frontal surface resulting in very high throughput and keeping the bed volume small. Typical process data show that operation time can be reduced by about 80% and buffer reduction can reach 95%. Protein loading in FT can be two orders of magnitude higher than with columns 6, 7.

Comparison of Q packed bed chro matography and Q Membrane Adsorber6

Q Resin Q Membrane Adsorber

Flow rate 100–150 cm/h 450–600 cm/h

Protein loading (flow through) 50–100 g/l >3,000 g/m2 or >10.9 kg/l

Buffer used 100% 5%

Cleaning validation Yes No

6 J.X. Zhou, T. Tressel (Amgen Inc.), Membrane Chromatography as a Robust Purification System for Large-Scale Antibody Production, BioProcess Int. 09, 2005, p. 32–37.

7 H. Knudsen et al. Membrane Ion Exchange Chromatography for Process-Scale Antibody Purification, J. Chromatography A 907, 2001, 145–154

Virus clearanceThis study5 also demonstrates excellent virus log reduction values with Sartobind Q. This enables membrane adsorber technology to replace a Q column without compromising critical viral clearance capabilities.

Murine Pseudorabies Reo Virus Minute Virus Leukemia Virus type 3 of Mice Virus (MuLV) (PRV) (Reo-3) (MVM)

50 ml virus > 5.57 ± 0.25 > 5.67 ± 0.17 > 7.28 ± 0.30 > 6.77 ± 0.24 spiked protein sample

500 ml virus 6.29 ± 0.25 > 5.67 ± 0.23 > 7.53 ± 0.29 4.41 ± 0.37 spiked protein| buffer solution

5 R. Zhang (Abgenix Inc.) et al, Viral Clearance Feasibility Study with Sartobind Q Membrane Adsorber for Human Antibody Purification, BioProduction 2004, Munich Germany 26–27 October 2004.

Fig. 5: No size exclusion effects for viruses

Fig. 6: Oversized column compared to Sartobind capsule for polishing application

Capsules vs. Columns

6

Dimensions and connections

Capsule with 4 mm pico nano mini 5” 10”, 20”, 30” mega bed height

Connector Luer Lock Luer Lock Sanitary 3’’ Sanitary 11’’ Sanitary 11’’ Sanitary 1’’

Capsule 31+ 11 mm 37+ 33 mm 87+45 mm 270+70 mm 365+100 mm (10”) 910+190 mm (height+diameter) 620+100 mm (20”) 870+100 mm (30”)

Inlet connector female female 25 mm outer d 50.5 mm outer d 50.5 mm outer d 50.5 mm outer d 10 mm inner d 15 mm inner d 36 mm inner d 36 mm inner d

Outlet connector as above as above as above 50.5 mm outer d as above as above 19 mm inner d

Hose barb version

Capsule (height+width) n. a. n. a. 103+45 mm 190+70 mm n. a. n. a.

Hose barb connectors n. a. n. a. 6–12 mm 14.5 mm n. a. n. a.

Recommended internal n. a. n. a. 6–10 mm 12 mm n. a. n. a. diameter of flexible tube

n.a. = not available Related products are Sartobind MA units for laboratory use and the Sartobind System Jumbo line with 8 mm bed height (Sartobind nano 3 ml, 150 ml and Jumbo 5 l) with void volume optimization for bind & elute and polishing.

Technical data

Membrane

Base material Stabilized reinforced cellulose

Membrane thickness 275 µm

Pore size (nominal) >3 µm

Ion exchanger types – strong anion Q (quaternary ammonium) R-CH2-N+(CH3)3

– strong cation S (sulfonic acid) R-CH2-SO3-

– anion exchanger (primary amine) -NH2

Capsule materials

Outer cage, inner core, Polypropylene end caps, capsule housing, nonwovens

O-ring in vent valve* Silicone Bed height 4 mm

* only with 10”, 20”, 30” and mega capsules

Operation

Depyrogenation 1 N NaOH 30–60 min at 20°C

Autoclaving 121°C for 30 minutes for one cycle only

Maximum operating pressure 4 bar, 0.4 MPa, 58 psi 5 bar, 0.5 MPa, 72.5 psi (Sartobind STIC PA 5“)

Fig. 7: Sartobind pico 0.08 ml for process development and virus studies

Fig. 8: Sartobind Q SingleSep nano 1 ml

Ordering Information

Sartobind Q capsulesOrder no. Chemistry Description Quantity Bed Bed Nominal Frontal Protein Recom- Approx. adapter inlet volume height membrane surface* binding mended Capsule and outlet [ml] [mm] area area capacity** flow rate weight [cm2] [g] [l/min]Sartobind pico92IEXQ42DD-11--D Q Luer female 10 0.08 4 2.9 cm2 0.19 0.002 0.002 1.5

Sartobind nano92IEXQ42DN-11 Q Luer female 1 1 4 36 cm2 2.4 0.029 0.03 10 g

92IEXQ42DN-11--A Q Luer female 4 1 4 36 cm2 2.4 0.029 0.03 10 g

Sartobind mini92IEXQ42D4-OO--A Q hose barb 4 7 4 250 cm2 20 0.2 0.2 30 g

92IEXQ42D4-SS--A Q sanitary flange 4 7 4 250 cm2 20 0.2 0.2 30 g

Sartobind 5 inch92IEXQ42D9-OO--A Q hose barb 4 70 4 2,500 cm2 160 2 1.9 220 g

92IEXQ42D9-SS--A Q sanitary flange 4 70 4 2,500 cm2 160 2 1.9 230 g

Sartobind 10 inch92IEXQ42D1-SS Q sanitary flange 1 180 4 6,600 cm2 450 5.3 5.0 800 g

Sartobind 20 inch92IEXQ42D2-SS Q sanitary flange 1 360 4 1.3 m2 900 10.5 10 1.4 kg

Sartobind 30 inch92IEXQ42D3-SS Q sanitary flange 1 540 4 2 m2 1,350 15.8 15 2 kg

Sartobind mega92IEXQ42DC3SS Q sanitary flange 1 1620 4 6 m2 4,050 48 50 5.6 kg

* Frontal surface area = average cylinder surface ** Typical dynamic binding capacity at 10% breakthrough was measured with BSA for Q | PA and lysozyme for S chemistry.

For Sartobind STIC PA 150 mM NaCl was added to the buffer.

7

8

Sartobind STIC capsulesOrder no. Chemistry Description Quantity Bed Bed Nominal Frontal Protein Recom- Approx. adapter inlet volume height membrane surface* binding mended Capsule and outlet [ml] [mm] area area capacity** flow rate weight [cm2] [g] [l/min]Sartobind pico92STPA42DD-11--D STIC PA Luer female 10 0.08 4 2.9 cm2 0.19 0.004 0.002 1.5 g

Sartobind nano92STPA42DN-11--A STIC PA Luer female 4 1 4 36 cm2 2.4 0.050 0.03 10 g

Sartobind 5 inch92STPA42D9-FF--A STIC PA sanitary flange 4 70 4 2500 cm2 160 3.5 1.9 230 g

Sartobind 10 inch92STPA42D1-SS STIC PA sanitary flange 1 180 4 6600 cm2 450 9 5 800 g

Sartobind 30 inch92STPA42D3-SS STIC PA sanitary flange 1 540 4 2 m2 1,350 27 15 2 kg

Sartobind mega92STPA42DC3SS STIC PA sanitary flange 1 1620 4 6 m2 4,050 81 50 5,6 kg

Sartobind S capsulesSartobind pico92IEXS42DD-11--D S Luer female 10 0.08 4 2.9 cmÇ 0.19 0.002 0.002 1.5

Sartobind nano92IEXS42DN-11 S Luer female 1 1 4 36 cm2 2.4 0.025 0.03 10 g

92IEXS42DN-11--A S Luer female 4 1 4 36 cm2 2.4 0.025 0.03 10 g

Sartobind mini92IEXS42D4-OO--A S hose barb 4 7 4 250 cm2 20 0.175 0.2 30 g

92IEXS42D4-SS--A S sanitary flange 4 7 4 250 cm2 20 0.175 0.2 30 g

Sartobind 5 inch92IEXS42D9-OO--A S hose barb 4 70 4 2,500 cm2 160 1.75 1.9 220 g

92IEXS42D9-SS--A S sanitary flange 4 70 4 2,500 cm2 160 1.75 1.9 230 g

Sartobind 10 inch92IEXS42D1-SS S sanitary flange 1 180 4 6,600 cm2 450 4.6 5.0 800 g

Sartobind 30 inch92IEXS42D3-SS S sanitary flange 1 540 4 2 m2 1,350 15.8 15 2 kg

* Frontal surface area = average cylinder surface ** Typical dynamic binding capacity at 10% breakthrough was measured with BSA for Q | PA and lysozyme for S chemistry.

For Sartobind STIC PA 150 mM NaCl was added to the buffer.

9

AccessoriesOrder no. Description5ZGI--0001 Stainless steel holder for one 10, 20 or 30 inch capsule, 3 legs

5ZGLG-0004 Stainless steel holder for three 10, 20 or 30 inch capsules, 3 legs

5ZALB-0002 Stainless steel distribution adapter for three 10, 20 or 30 inch capsules

9ZAIAM0001 Stainless steel legs for mega, 3 items

1ZAOGV0003 Adapter Sanitary 25 mm 1–3“ to UNF10–32 to connect mini to LC systems, 2 items

1ZA---0004 Adapter Luer male to UNF – 10–32 female, PEEK, 1 item

Fig. 10: A Sartobind mega capsule on stainless steel legs

Fig. 9: Adapter to connect mini with sanitary connection to liquid chromatography systems

Sales and Service ContactsFor further contacts, visit www.sartorius-stedim.com

EuropeGermanySartorius Stedim Biotech GmbHAugust-Spindler-Strasse 1137079 Goettingen

Phone +49.551.308.0Fax +49.551.308.3289

Sartorius Stedim Systems GmbHRobert-Bosch-Strasse 5 – 7 34302 Guxhagen

Phone +49.5665.407.0Fax +49.5665.407.2200

FranceSartorius Stedim FMT S.A.S.ZI des PaludsAvenue de Jouques – CS 9105113781 Aubagne Cedex

Phone +33.442.845600Fax +33.442.845619

Sartorius Stedim France SASZI des PaludsAvenue de Jouques – CS 7105813781 Aubagne Cedex

Phone +33.442.845600Fax +33.442.846545

AustriaSartorius Stedim Austria GmbHFranzosengraben 121030 Vienna

Phone +43.1.7965763.18Fax +43.1.796576344

BelgiumSartorius Stedim Belgium N.V.Leuvensesteenweg, 248/B1800 Vilvoorde

Phone +32.2.756.06.80Fax +32.2.756.06.81

HungarySartorius Stedim Hungária Kft.Kagyló u. 52092 Budakeszi

Phone +36.23.457.227Fax +36.23.457.147

ItalySartorius Stedim Italy S.p.A.Via dell’Antella, 76/A50012 Antella-Bagno a Ripoli (FI)

Phone +39.055.63.40.41Fax +39.055.63.40.526

NetherlandsSartorius Stedim Netherlands B.V.

Phone +31.30.60.25.080Fax +31.30.60.25.099

filtratie.nederland@sartorius-stedim.com

PolandSartorius Stedim Poland Sp. z o.o.ul. Wrzesinska 7062-025 Kostrzyn

Phone +48.61.647.38.40Fax +48.61.879.25.04

Russian FederationLLC “Sartorius ICR”Uralskaya str. 4, Lit. B199155, Saint-Petersburg

Phone +7.812.327.5.327Fax +7.812.327.5.323

SpainSartorius Stedim Spain, S.A.U.Avda. de la Industria, 32Edificio PAYMA28108 Alcobendas (Madrid)

Phone +34.902.110.935Fax +34.91.358.96.23

SwitzerlandSartorius Stedim Switzerland AGRingstrasse 24 a8317 Tagelswangen

Phone +41.52.354.36.36Fax +41.52.354.36.46

U.K.Sartorius Stedim UK Ltd.Longmead Business CentreBlenheim Road, EpsomSurrey KT19 9 QQ

Phone +44.1372.737159Fax +44.1372.726171

AmericaUSASartorius Stedim North America Inc.5 Orville Drive, Suite 200Bohemia, NY 11716

Toll-Free +1.800.368.7178Fax +1.631.254.4253

ArgentinaSartorius Argentina S.A. Int. A. Ávalos 4251 B1605ECS Munro Buenos Aires

Phone +54.11.4721.0505 Fax +54.11.4762.2333

BrazilSartorius do Brasil Ltda Avenida Senador Vergueiro 2962 São Bernardo do Campo CEP 09600-000 - SP- Brasil

Phone +55.11.4362.8900Fax + 55.11.4362.8901

MexicoSartorius de México S.A. de C.V.Circuito Circunvalación Poniente No. 149Ciudad Satélite53100, Estado de MéxicoMéxico

Phone +52.5555.62.1102Fax +52.5555.62.2942

Asia|PacificAustraliaSartorius Stedim Australia Pty. Ltd.Unit 5, 7-11 Rodeo DriveDandenong South Vic 3175

Phone +61.3.8762.1800Fax +61.3.8762.1828

ChinaSartorius Stedim Biotech (Beijing) Co. Ltd.No. 33 Yu’an RoadAirport Industrial Park Zone BShunyi District, Beijing 101300

Phone +86.10.80426516Fax +86.10.80426580

Sartorius Stedim Biotech (Beijing) Co. Ltd.Shanghai Branch Office3rd Floor, North Wing, Tower 1No. 4560 Jin Ke RoadPudong District, Shanghai 201210

Phone +86.21.68782300Fax +86.21.68782332 | 68782882

Sartorius Stedim Biotech (Beijing) Co. Ltd.Guangzhou Representative OfficeUnit K, Building 23Huihua Commerce & Trade BuildingNo. 80 Xianlie Middle RoadGuangzhou 510070

Phone +86.20.37618687 | 37618651Fax +86.20.37619051

IndiaSartorius Stedim India Pvt. Ltd.#69/2-69/3, NH 48, JakkasandraNelamangala Tq562 123 Bangalore, India

Phone +91.80.4350.5250Fax +91.80.4350.5253

JapanSartorius Stedim Japan K.K.4th Fl., Daiwa Shinagawa North Bldg.8-11, Kita-Shinagawa 1-chomeShinagawa-ku, Tokyo, 140-0001 Japan

Phone +81.3.4331.4300Fax +81.3.4331.4301

MalaysiaSartorius Stedim Malaysia Sdn. Bhd.Lot L3-E-3B, Enterprise 4Technology Park MalaysiaBukit Jalil57000 Kuala Lumpur, Malaysia

Phone +60.3.8996.0622Fax +60.3.8996.0755

SingaporeSartorius Stedim Singapore Pte. Ltd.1 Science Park Road, The Capricorn, #05-08A,Singapore Science Park IISingapore 117528

Phone +65.6872.3966Fax +65.6778.2494

South KoreaSartorius Korea Biotech Co., Ltd.8th Floor, Solid Space B/D, PanGyoYeok-Ro 220, BunDang-GuSeongNam-Si, GyeongGi-Do, 463-400

Phone +82.31.622.5700Fax +82.31.622.5799

www.sartorius-stedim.com

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Sales and Service ContactsFor further contacts, visit www.sartorius-stedim.com

EuropeGermanySartorius Stedim Biotech GmbHAugust-Spindler-Strasse 1137079 Goettingen

Phone +49.551.308.0Fax +49.551.308.3289

Sartorius Stedim Systems GmbHRobert-Bosch-Strasse 5 – 7 34302 Guxhagen

Phone +49.5665.407.0Fax +49.5665.407.2200

FranceSartorius Stedim FMT S.A.S.ZI des PaludsAvenue de Jouques – CS 9105113781 Aubagne Cedex

Phone +33.442.845600Fax +33.442.845619

Sartorius Stedim France SASZI des PaludsAvenue de Jouques – CS 7105813781 Aubagne Cedex

Phone +33.442.845600Fax +33.442.846545

AustriaSartorius Stedim Austria GmbHFranzosengraben 121030 Vienna

Phone +43.1.7965763.18Fax +43.1.796576344

BelgiumSartorius Stedim Belgium N.V.Leuvensesteenweg, 248/B1800 Vilvoorde

Phone +32.2.756.06.80Fax +32.2.756.06.81

HungarySartorius Stedim Hungária Kft.Kagyló u. 52092 Budakeszi

Phone +36.23.457.227Fax +36.23.457.147

ItalySartorius Stedim Italy S.p.A.Via dell’Antella, 76/A50012 Antella-Bagno a Ripoli (FI)

Phone +39.055.63.40.41Fax +39.055.63.40.526

NetherlandsSartorius Stedim Netherlands B.V.

Phone +31.30.60.25.080Fax +31.30.60.25.099

filtratie.nederland@sartorius-stedim.com

PolandSartorius Stedim Poland Sp. z o.o.ul. Wrzesinska 7062-025 Kostrzyn

Phone +48.61.647.38.40Fax +48.61.879.25.04

Russian FederationLLC “Sartorius ICR”Uralskaya str. 4, Lit. B199155, Saint-Petersburg

Phone +7.812.327.5.327Fax +7.812.327.5.323

SpainSartorius Stedim Spain, S.A.U.Avda. de la Industria, 32Edificio PAYMA28108 Alcobendas (Madrid)

Phone +34.902.110.935Fax +34.91.358.96.23

SwitzerlandSartorius Stedim Switzerland AGRingstrasse 24 a8317 Tagelswangen

Phone +41.52.354.36.36Fax +41.52.354.36.46

U.K.Sartorius Stedim UK Ltd.Longmead Business CentreBlenheim Road, EpsomSurrey KT19 9 QQ

Phone +44.1372.737159Fax +44.1372.726171

AmericaUSASartorius Stedim North America Inc.5 Orville Drive, Suite 200Bohemia, NY 11716

Toll-Free +1.800.368.7178Fax +1.631.254.4253

ArgentinaSartorius Argentina S.A. Int. A. Ávalos 4251 B1605ECS Munro Buenos Aires

Phone +54.11.4721.0505 Fax +54.11.4762.2333

BrazilSartorius do Brasil Ltda Avenida Senador Vergueiro 2962 São Bernardo do Campo CEP 09600-000 - SP- Brasil

Phone +55.11.4362.8900Fax + 55.11.4362.8901

MexicoSartorius de México S.A. de C.V.Circuito Circunvalación Poniente No. 149Ciudad Satélite53100, Estado de MéxicoMéxico

Phone +52.5555.62.1102Fax +52.5555.62.2942

Asia|PacificAustraliaSartorius Stedim Australia Pty. Ltd.Unit 5, 7-11 Rodeo DriveDandenong South Vic 3175

Phone +61.3.8762.1800Fax +61.3.8762.1828

ChinaSartorius Stedim Biotech (Beijing) Co. Ltd.No. 33 Yu’an RoadAirport Industrial Park Zone BShunyi District, Beijing 101300

Phone +86.10.80426516Fax +86.10.80426580

Sartorius Stedim Biotech (Beijing) Co. Ltd.Shanghai Branch Office3rd Floor, North Wing, Tower 1No. 4560 Jin Ke RoadPudong District, Shanghai 201210

Phone +86.21.68782300Fax +86.21.68782332 | 68782882

Sartorius Stedim Biotech (Beijing) Co. Ltd.Guangzhou Representative OfficeUnit K, Building 23Huihua Commerce & Trade BuildingNo. 80 Xianlie Middle RoadGuangzhou 510070

Phone +86.20.37618687 | 37618651Fax +86.20.37619051

IndiaSartorius Stedim India Pvt. Ltd.#69/2-69/3, NH 48, JakkasandraNelamangala Tq562 123 Bangalore, India

Phone +91.80.4350.5250Fax +91.80.4350.5253

JapanSartorius Stedim Japan K.K.4th Fl., Daiwa Shinagawa North Bldg.8-11, Kita-Shinagawa 1-chomeShinagawa-ku, Tokyo, 140-0001 Japan

Phone +81.3.4331.4300Fax +81.3.4331.4301

MalaysiaSartorius Stedim Malaysia Sdn. Bhd.Lot L3-E-3B, Enterprise 4Technology Park MalaysiaBukit Jalil57000 Kuala Lumpur, Malaysia

Phone +60.3.8996.0622Fax +60.3.8996.0755

SingaporeSartorius Stedim Singapore Pte. Ltd.1 Science Park Road, The Capricorn, #05-08A,Singapore Science Park IISingapore 117528

Phone +65.6872.3966Fax +65.6778.2494

South KoreaSartorius Korea Biotech Co., Ltd.8th Floor, Solid Space B/D, PanGyoYeok-Ro 220, BunDang-GuSeongNam-Si, GyeongGi-Do, 463-400

Phone +82.31.622.5700Fax +82.31.622.5799

www.sartorius-stedim.com