Compound Light Microscope Uses light Image appears upside down
and backwards Under Low Power field of view is larger (4 times)
Under High Power field of view is smaller (4 times)
Slide 4
How will the f appear in the field of view of a compound
microscope?
Slide 5
Upside Down and Backwards
Slide 6
FOV in Low Power: 4 cells are visible How many cells will be
visible in high power?
Slide 7
FOV in High Power: 1 cell is visible!
Slide 8
Microscope Rules: Always start with low power Field of view is
large and bright Helps you find specimen Use the coarse adjustment
knob to focus
Slide 9
Switch to High Power to see detail Adjust diaphragm (light) for
smaller field of view Field of view is smaller and darker Helps see
fine detail Only use fine adjustment knob to focus
Slide 10
Total Magnification: Eyepiece # X Objective # = Total
Magnification # What is the highest total magnification in this
diagram? Answer: 10 X 40 = 400x Eyepiece 10x 40x 10x
Slide 11
What is the total magnification under low power in the diagram?
Eyepiece 10x 40x 10x Answer: 10 X 10 = 100x
Slide 12
Electron Microscope A major breakthrough because they can
magnify 250,000 xs Shows much more detail than compound light
microscopes Shows great detail of inner structures Ex) The inside
of mitochondria in a cell!
Slide 13
Microdissection tools/instruments Special micro-tools (very
tiny) Used to take apart cells Ex) Removing a nucleus from one cell
and putting inside another cell! Used in genetic engineering
Slide 14
Use of Staining Staining allows for cell parts to be seen The
nucleus for example becomes more visible under the microscope when
a stain is used Iodine is often used to stain cells
Slide 15
Centrifuge (Ultracentrifuge) Spins at high speeds to separate
substances by density Heavier particles end up in the bottom of the
test tube
Slide 16
Chromatography Separates colors Chromatography paper is used
Ex) Plant pigments can be separated using chromatography
Slide 17
Gel Electrophoresis Used to separate bands of DNA DNA bands of
different sizes move at different rates DNA bands move because of
the charge Used in forensic investigation (CSI) Also used to
determine paternity
Slide 18
Gel Electrophoresis How do you know these samples are from
different people? Whats responsible for the movement of DNA?
Slide 19
Which two species of bear are most similar? How do you
know?
Slide 20
Which direction should the slide be moved to center the
cell?
Slide 21
The diagram below shows the letter h under low power, which
diagram shows the high power field of view? 1. 2.3.4.
Slide 22
How many micrometers in diameter does each cell measure?
Slide 23
How many millimeters in diameter does each cell measure?
Slide 24
Slide 25
In which direction should the slide be moved to center the
cell?
Slide 26
Name 2 unsafe practices shown below.
Slide 27
Which lab technique was used to separate the substances in the
test tube?