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Screening, identification and characteristics
of a quinclorac degrading strain Arthrobacter
sp. MC-10
CHEN De-xin Ph.D.
Qingzhou Tobacco Research Institute of CNTC
Email:[email protected]
Tel:(86)532 66715507 Fax:(86)532 88701032
15 Oct 2014 Québec Canada
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Occurrence of tobacco quinclorac injury
Control methods of tobacco quinclorac injury
Research on quinclorac degrading bacteria
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Screening, identification and characteristics of a quinclorac
degrading strain Arthrobacter sp. MC-10
Main Content
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1 Occurrence of tobacco quinclorac injury
1.1 Quinclorac Profile
Formula: C10H5CI2NO2;
Name: 3,7-dichloroquinoline-8- carboxylic aeid
Hormone type herbicides, mainly used in paddy
Thermal stability, light stability, stable in acidic medium
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1 Occurrence of tobacco quinclorac injury
1.2 Long vinca, serious impact
Half-life is 35.6 d
No significant effect on the growth of
tobacco after 269 d
Injury symptoms: Leaves curl back,
become narrow and leathery; In severe
cases, loss of economic value
Residues
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2 Control methods
Physical control: Activated carbon adsorption
Chemical control: Quicklime neutralize
Pharmacy management: Ultra-sensitive protein, Brassinolide
Cultivation measures: Crop rotation, deep overburden
Control methods can not prevent injury effectively
How to control tobacco quinclorac injury more simple and
rapidly
Degrading bacteria filter
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3 Research of quinclorac degrading bacteria
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3.1 Screening of degrading bacteria MC-10
100mL 50mg/L
quinclorac
inorganic salt
culture
Add in
28℃, 150
r/minculti
vatd 7d
Adapter
50mg/L
quinclorac
inorganic
salt culture
5% inoculum 5g soil
samples
Separation of
pure strains
enrichment culture four
times
Expand
trainning in
LB culture
Preliminary get 12
degrading bacteria
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3.2 Determination of degradation
High performance
liquid chromatography
(HPLC)
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3.2 Determination of degradation
Strain
No.
Quinclorac
concentration
after 7 days
(mg/L)
Degradation
rate Strain No.
Quinclorac
concentration
after 7 days
(mg/L)
Degradatio
n rate
MC-01 15.15 23.7% MC-07 15.81 20.4%
MC-02 16.51 16.9% MC-08 5.19 73.5%
MC-03 17.03 14.3% MC-09 17.85 10.2%
MC-04 17.99 9.5% MC-10 1.63 91.3%
MC-05 6.07 69.1% MC-11 8.51 56.9%
MC-06 8.25 58.2% MC-12 11.35 42.7%
CK 19.89 -
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3 Research of quinclorac degrading bacteria 3.3 HPLC chromatograms of MC-10
MC - 10
degradation
quinclorac
Control quinclorac
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3.4 Identification of MC-10
Colony morphology of MC-10
Morphology
Electron microscopy
Molecular Biology
Arth
robacte
r sp.
TEM images of
MC-10
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MC-10 (KJ619485)
Arthrobacter mysorens (JDXVZ4J3015)
Arthrobacter arilaitensis (JDWR1MZG014)
Arthrobacter bergerei (JDXE6GBR014)
Arthrobacter gangotriensis (JDXMGYCS015)
Arthrobacter woluwensis (JDX71G7Y014)
Arthrobacter globiformis (JDXTH1W9014)
Arthrobacter stackebrandtii (JDX9A2VP014)
Arthrobacter phenanthrenivorans (JDXE5NUD015)
Arthrobacter chlorophenolicus (JDXGT54S014)
Arthrobacter aurescens (JDXB2BMZ014)
Arthrobacter nicotinovorans (JDXX0VJU015)
Arthrobacter crystallopoietes (JDXJ4KJ2015)
Arthrobacter luteolus (JDXUVES7014)
Arthrobacter castelli (JDXFJWV3015)
Arthrobacter cumminsii (JDXKFYRN014)
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99
98
100
97
66
67
79
52
0.005
MC-10 (KJ619485)
Phylogenetic tree of Strain MC-10
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3 Research of quinclorac degrading bacteria
3.5 Characterization of MC-10
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Optimum degradation
temperature:30℃
Optimum degradation
pH:7
Optimum inoculum:5%
Optimum
degradation
conditions
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3.5 Characterization of MC-10 2014
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Reduce the toxic effects on root, promote root
development
Alleviate the effects of quinclorac injury, alleviate
leaf curl
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3.6 Alleviate the effects of quinclorac injury
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A:Normal tobacco plants; B:Toxic soil and MC-10;
C: Toxic soil
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1. Field Application of MC-10
2. Industrialized breeding
2 Problem
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