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SE3D Education Bioprinting for next generation STEM students
SE3D Education
Bioprinting for next generation STEM students
History of Bioprinting
1983 – Charles Hull invented stereolithography
1990 – Gabor Forgacs explored the use of 3D printing into tissue engineering
1999 – Wake Forest Institute of Regenerative Medicine used a 3D printer to build a synthetic scaffold of a human bladder
2003 – Inkjet printer used to deposit biological materials (Thomas Boland)
2007 – Organovo, the first bioprinting company, was founded
2009 – The first commercial bioprinter, NovoGen MMX was introduced
From CAD to Object
3D printing
Bioprinting
© Copyright 2016 SE3D Education
How does a bioprinter work?
z
x y
Bio-ink
Bioprinting process
polymer
hydrogel
cells
in vitro tissue
Bioprinting© Copyright 2016 SE3D Education
BIOPRINTING APPLICATIONS
Bioprinted
Organs
Medical
Devices
Artificial
Tissue
Biofuels
Drug
Discovery
Meat
Production
Introduction© Copyright 2016 SE3D Education
r3bEL – The Classroom Bioprinter
1. Engage students in exciting and cutting-edge science
2. Increase hands-on learning for every student
3. Provide opportunities for students to innovate in the biosciences
© Copyright 2016 SE3D Education
Bioink: hydrogels for bioprinting
Alginate gelation
Drug delivery Wound dressing
Hydrogels are network of polymer chains containing >90% water.• Natural
- alginate, agarose, collagen
• Synthetic- pAAm, PEG
• Preparation: chemical / thermal crosslinking
Applications:
Bioink© Copyright 2016 SE3D Education
Enzymes and their applicationsWhat are enzymes?
Enzymes are proteins that act as catalysts and
facilitate complex biochemical reactions
Applications: Screening for kinase inhibitors as
potential cancer drugs
Introduction to Enzymes
ABTS ABTS* (oxidized) (substrate) (Blue product)
HRP (enzyme)
Example:
HER2 kinase inhibitors
reduce the overall HER2
activity &tumor growth
© Copyright 2016 SE3D Education
Today’s activity: Bioprinted enzyme arrays
… . … . … . … . … . … .
Top view
Side viewR3BEL Side view
Top view
Step 1: Setup printer Step 2: Print enzymes Step 3: Print substrate Step 4: See reaction!!
Lab activity© Copyright 2016 SE3D Education
Other Workshops for TeachersOur Commitment to Teachers
Enzyme Lab
Algae Lab
E. Coli Lab
Chocolate Printing
3D Modeling
- Creating enzyme arrays to learn more about how enzymes work
- Creating algae arrays to understand photosynthesis
- Creating bacteria arrays to understand how antibiotics are developed
- Printing chocolate to learn more about chocolate chemistry
- 3D tools for creating models for printing
For more information about our workshops, visit us at http://www.se3d.com/workshops.html
Bioprinted algae arrays
RESULTS
Lab activity
DATA ANALYSIS
© Copyright 2016 SE3D Education
Be in the Forefront of Classroom Technology Innovation
Bioprinter: $2,500 (pilot)
• Compact and lightweight
• Heated platform 25 – 80 °C
• Heated extruder 25 – 100 °C
• Syringe volume 5 mL
• Syringe needle sizes 14 – 28 G
Available for one-year minimum lease $95/month
1. BioTech Starter Kit• Alginate
• HRP (enzyme)
• ABTS/H2O2 (substrates)
2. GreenTech Starter Kit • Agarose
• Algae
• Bicarbonate indicator
Each kit makes 25-100 experiments
BioKits: $75-250
To request more information or a quotation, please email us [email protected]
SE3D Bioprinting Curriculum Tracks
BIOPRINTING LAB MODULES
Track IIGreen Technology
Track IPharmaceutical
Track IIITissue Engineering
EnzymesBasics AlgaeProteins Bacteria
CurriculumFor more information about our curriculum, email us [email protected]
Pilot School ProgramSpring/Fall 2016• 25% on bioprinter purchase ($2500 only)• Exclusive preview of full curriculum• 10% discount on first year curriculum• Publicity and media coverage via SE3D
marketing/PR campaigns• Free consultation of personalized learning• Invitation of our bioprinting conference 2017 • Be the school that LEADS
To participate in our pilot program, email Dr. Maya Lim directly [email protected]
