Sealing - BioTools · Power: 300W Sealing Temp: 60-200°C Sealing Time: 0 - 10 seconds Connection:...

You can choose between adhesive and heat sealing solutions. Both offer a wide selection of materials to choose from depending on your application requirements. See page 83 for application dependent selection of adhesive seals and page 73 for heat seals.

All 4titude® seals are certified free from nucleases and human genomic DNA.

Adhesive Seals:

Most adhesive seals are supplied with convenient tabs on both ends for easy application. These tabs also enable easy peeling to remove a seal without leaving adhesive residue on the sealing surface. For best sealing results we offer a roller, see page 53 to ensure even pressure is applied, for complete and secure application of all our adhesive seals.

Heat Seals:

Heat seals are available as sheets, for manual or semi-automatic heat sealers, as well as in roll formats for automated sealers. Heat sealing offers 100% effective sealing integrity and is a quick and cost-effective sealing solution.

Depending on throughput we recommend our semi-automatic 4s3 heat sealer page 64 or automated A4S sealer page 62 for applying your heat seal.

Sealing4titude® offers the widest range of plate sealing solutions on the market.

All seals are certified free from nucleases and human genomic DNA

| www.4ti.co.uk60 +44 (0)1306 884885 | www.4ti.co.uk60 +44 (0)1306 884885

Heat SealingHeat sealing is the gold standard plate sealing method, minimising sample loss and maximising sample security, for PCR, qPCR, storage and other applications.

IMAGES?

The sealing performance of heat seals is superior to cap, mat and adhesive sealing with clear reductions in sample loss and cross contamination. The optimized sealing performance allows use of smaller reaction volumes leading to reagent savings.

Heat sealing is the most cost efficient way to seal plates for a wide range of applications. Per well comparisons show a 3 to 5 five fold reduction in costs for qPCR application (list price comparison of Clear Weld Seal against caps and qPCR seal) and at least a 50% reduction for storage solutions (list price comparison of Peel Seal against caps and PCR seal). Costs can be further reduced by changing to use of the material format, using rolls rather than sheets.

The application of a heat seals is much faster than caps or adhesive seals. Typical sealing times are around 2,5 seconds. Depending on throughput the use of a semi-automatic or fully automatic heat sealer can guarantee a reproducible sealing quality by standardising the sealing parameters, time, temperature and pressure. Sealing performance variability between different users can be eliminated.

4titude® offers a wide choice of heat seals in both roll and sheet format. Different seals can be chosen to optimize the use depending on plate material, desired permeability or resistance, ability to peel or pierce the seal material and visualisation through the material. Please refer to the comparison table on page 73 for an overview of our heat sealing range.

All seals are certified free from nucleases and human genomic DNA contamination.

The best sealing results can be obtained by using flat, rigid plates with a raised rim design like our FrameStar® 2-component plates and reliable, high quality sealing instruments such as the 4s3 semi-automated heat sealer or the 4AS sealer.

For details of the instruments see page 62 and 64. For a demonstration or trial of a heat sealer please contact us at [email protected].

www.4ti.co.uk | 61 +44 (0)1306 884885 www.4ti.co.uk | 61 +44 (0)1306 884885

Automatic Heat Sealer A4SThe A4S automatic heat sealer completes our heat sealing range by providing the perfect solution for automated heat sealing of medium to high-throughput or full automation applications.

Crucially the A4S does not require an air supply allowing for flexible use on standard lab benches as stand-alone unit, or integrated within a robotic set-up. The sealer has a rapid heating element, for fast start-up, and has a stand-by function, to save energy when not in use.

The A4S sealer works with a wide range of plates from 384well and 24well PCR plates, to deep well or storage plates with an SBS footprint. Different plate heights, from 3mm to 60mm, can be accommodated with minimal adjustments on the instrument. Plates are presented in portrait format and the plate position on the shuttle allows good access for robotic integration.


Feature:

• No air supply needed

• Colour-Touch-screen/Institutive user interface

• Saved sealing protocols

• Auto stand-by function to save energy

• Compatible with a wide range of the plate types, including 1536well PCR plates

• Rapid heating element for fast start-up

• Optional cover

• 2 roll positions depending on available space and roll size

• Sealing cycle time of less than 15sec

• Up to 5000 seals without manual intervention

Standard size, 78mm width rolls, can be loaded in either of two roll positions depending on roll length and available space enabling the entire instrument to fit under the gantree of many liquid handling robots.

The A4S doesn’t produce any sealing material waste. The roll width covers an SBS footprint microplate and the seal is cut at the end of the plate leaving only minimal overhangs to allow for easy removal of the seal, but not interfering with plate stacking.

For our range of sealing materials see page 66 to 72. Sample rolls are available on request.

Technical Specification

Weight: 25 kg

Voltage: 110/230V

Maximum power consumption: 750W

Power saving mode: less than 20W

Sealing Temp: 120-200C

Sealing Time: 0-10sec

Connection: RS-232 serial port The modern user interface has a colour touchscreen, intuitive controls and can save individual sealing protocols for future use.

4ti-0665 A4S Automatic Sealer 1

Code Description Quantity


Features and Benefits

• Automaticsealingprocessforunmatchedsealingconsistency

• Variabletemperatureandtimesettingsforeasyoptimisationofsealingconditions

• Simple,singlebuttonoperation

• Rapidheatingelementforfaststart-up

• Real-timetemperaturedisplay

• Autostand-byfunctiontosaveenergy

• Autoswitch-offfunctionforaddedsafety

• Compatiblewithwidevareityofconsumables

• RS-232serialportenablesunittobeintegratedintoautomatedsystems

• Fullrangeofheatsealsavailable

• Smallfootprint

• Lowtemperaturesealingforusewithadhesiveseals

Comparedtolessautomatedheatsealersonthemarketthe4s3removesanyuservariationfromsealapplicationensuringareproduciblesealtimeandtimeagainandenablingconfidenceintheconsistencyofsealingresults.

Thepressureappliedduringsealingisconstantandthetimeandtemperaturecanbeadjusted.Thiswillenablethesealofchoicetobeattainedreproducibly.Aneasilypeelablesealorastrongsealforstoragecanbeachievedwithoutfearofunderoroversealing.

Damagetomicroplatescausedbyoversealingispreventedenablingrepeatedresealingofplates.

The4s3iscompatiblewithanystandardSBSfootprintplateandcanalsobeusedtosealrackedtubes/tubestrips.

4titude®offersthelargestrangeofheatsealingmaterialsavailable,withpeelable,piercableandpermeablesealingfilmandfoils.

Forademonstrationortrialofa4s3pleasecontact4titudeoryourlocaldistributor.

set temperature

1

set time

2

press “operate”

4

load plate & seal

3

plate is sealed automatically

5

remove sealed plate

6

4s3The4s3isasemi-automaticheatsealercompatiblewithawiderangeofsealingmaterialsandplatesincludingPCR,qPCR,assayandstorageplates.

NEW


Heat Sealing

Plate FormatsThe4s3iscompatiblewithalltypesofSBSfootprintplates:

• SBSstandardfootprintpolypropylene&polystyreneplates,useadapter4ti-0615

• SBSdeep-wellplates(96or384-well),useadapter4ti-0615

• 96wellPCRplates(skirted,semi-skirtedornon-skirted),useadapter4ti-0625

Adapters and Sealing AidsThe4s3employsauniqueandcost-savingadaptersystem.Deep-wellplatesandmostotherskirtedplatescanbesealedonthestandardplatecarrier(4ti-0615,suppliedwiththeunit).Forall96wellPCRplatesegmentsandtubestrips,anadditionalplatecarrier(4ti-0625,optionalextra)mustbeused.Bothplatecarrierscanbecombinedforeasierhandlingofshallowwellplates,suchas384-wellPCRplates.

Forimprovedsealingoffilmsorfoilsthathaveatendencytocurl,weoffersealingaids.Aweightedplaten(4ti-0602)forusewithdeep-wellplatesissuppliedwiththeunitandasealingframeforusewithallotherplatedesignsissuppliedwhenorderingtheoptional4ti-0625adapter.

Real Time DisplayTherapidheatingelementallowsforaquickstart-upwithinminutes.Thetemperatureisdisplayedinreal-timesotheheating-upprocesscanbemonitored.

Energy Saving FunctionsWhentheunitisleftidle,itautomaticallyswitchesintostand-bymode,duringwhichthetemperatureoftheheatingelementisreducedto60°C,tosaveenergy.Whenthesealerisleftidleforlongerperiods,itswitchesoffautomatically,foraddedsafety.Thetimesatwhichtheunitswitchesintostand-byandswitch-offmodescanbeadjustedtosuittheusers’needs.

RS-232 Serial Port for Automated IntegrationTheadditionofaserialportatthebackoftheunitmeansthe4s3canbeintegratedintoanexistingLIMSsystemwhereittrulybecomesanautomated,walk-awayheatsealer.

The4s3isSILAcompatibleandadriverforplugandplayuseinintegrationsystemsisavailable

4ti-0625: 96-well PCR plate adapter

4ti-0615: Standard plate adapter

4ti-0615 & 4ti-0625 interlocked for easy handling of shallow well plates

4ti-0616: Roche 1536-well plate adapter

4ti-0612: Sealing frame

4ti-0616 & 4ti-0625 interlocked for easy handling of Roche 1536-well plates

4ti-0602: Weighted sealing platen

Real-time temperature display

Technical Specification

Weight:9kg

Dimensions:310 x 275 x 181mm (HxDxW)

Voltage:110/230V

Power:300W

SealingTemp:60-200°C

SealingTime:0 - 10 seconds

Connection:RS-232 Serial port

4ti-0655 4s3HeatSealer

Includesuniversalpowersupply,powercord,manual,standardplate

adapter(4ti-0615)andweightedsealplaten(4ti-0602)

4ti-0615 Adapterfordeep-welland384-wellPCRplates

4ti-0616 AdapterforRoche1536-wellPCRplates

4ti-0602 Weightedsealplatentoholddown

sealingsheetswithatendencytocurl

4ti-0625 Adapterfor96-wellPCRplates,includessealingframe(4ti-0612)

4ti-0612 Sealingframeforusewith4ti-0625adapter

Ordering Information

Sealing Accessories and Tools

Code Description

www.4ti.co.uk | 65 +44 (0)1306 884885

Hea

t Se

alin

g

PEELABLE

A

UTOCLAVABLE

121o

ClearsealisanopticallyclearfilmwhichformsaneasilypeelableyetPCR/qPCRtightseal.Itsopticalclarityallowsuseforallopticalapplications.Theclearsealcanbeusedtosealmostplatetypesincludingpolypropylene,polyethylene,polystyreneandCOCplates.Availableassheetsoronarollforautomatedsealers.

Clear Seal

Applications

• qPCRorotheropticalapplications(e.g.fluorescenceorcolorimetricassays)

• Shorttermcompoundstorage

• Suitableformostplatematerials

• Availableinsheetorrollformatforautomatedsealers

• FreefromDNase,RNaseandhumangenomicDNA

Clearweldsealisanopticallyclearpolymerfilmwhichformsapermanentbondtopolypropyleneplatesthatcannotbepeeledandisdifficulttopierce.Thesealhasagoodsolventresistanceforsafelongtermstorage.

Samplecanbeaccessedifrequiredusingabladeorneedle.

NowalsoavailableasFrameSealproductseepage75

Clear Weld Seal Mark II

Applications

• PCR

• waterbaththermalcyclers

• qPCRandotheropticalapplications(e.g.fluorescenceandcolorimetricassays)

• Storageanddisposalofhazardousmaterials

• RecommendedforusewithDMSO

• Availableassheetsorinrollformatforautomatedsealers


*(110Cwithpressurisedheatedlid)

SE

AL

INT

EG

R

ITY TEMPERATU

RE

RA

NG

E

-80°

80°

*

*Onceautoclaved,notrecommendedforPCRapplications

SE

AL

INT

EG

R

ITY TEMPERATU

RE

RA

NG

E

-80°

110°

*

Code Pack Size/Dimensions

4ti-0573 610mx78mmroll1Approx5000seals

4ti-0573S Trialroll15mx78mm



4ti-0575 100sheets(125mmx78mm)

4ti-0575/FS SealingFrame:50seals/frameperpack



4ti-0540/REMP 500mx78mmroll3Approx4200seals




4ti-0542S Trialroll

4ti-0541 100sheets(125mmx78mm)5mx115mm

NEW

| www.4ti.co.uk66 +44 (0)1306 884885

Heat Sealing

TheClearSeal3730isaclearpolyesterbackedfilmwhichisparticularlythin.Itsealstopolypropylene,polystyrene,polyethyleneandCOCmicroplates.Thissealhasgoodopticalclaritywithmoderatesolventresistance.Thethinnatureofthissealenablesittobeeasilypiercedwithanauto-samplerneedle,ortheprobefromtheABi3730sequencer.

Thesealisonlyavailableinrollformat,butarollwithperforatedsheetscanbeusedforlowthroughput.

Clear Seal 3730

Applications

• PCR

• qPCRandotheropticalapplications(e.g.fluorescenceandcolorimetricassays)

• Piercablebyautosamplesneedles

• PiercablebytheABI3730

• Availableinrollformatforautomatedsealers


PIERCEABLE

ThispopularlaminatematerialgivesapeelableheatsealtoPPandCOCplates.Itcanbepeeleddirectlyfromtheplatefollowingremovalfromthefreezerandisagoodbarriertoaqueoussolutions.Thesealshowsmoderateresistancetosolventsatroomtemperatureandformsaweld-sealtopolyethyleneplates(cannotbepeeled).

Peel Seal

Applications

• Verylowtemperaturecompoundstorage.

• Mid-term(<5days)roomtemperaturecompoundstorage

• PCR

• Availableassheetsorinrollformatforautomatedsealers


1 Compatible with Thermo Fisher ALPS 300™ / ALPS 3000™ /

KBiosystems Wasp™ / KBioscience FlexiSeal and Cube2 Compatible with Agilent (Velocity 11) PlateLoc®

3 Compatible with REMP Portrait Heat Sealer (PHS)4 Compatible with REMP Landscape / Stacking Heat

Sealers (LHS/SHS)

COC = Cyclic Olefin Copolymer

SE

AL

INT

EG

R

ITY TEMPERATU

RE

RA

NG

E

-20°

80°

*(110C with pressurised heated lid)

*

*(110CwithpressurisedPCRheatedlid)

A

UTOCLAVABLE

121o

PEELABLE

SE

AL

INT

EG

R

ITY TEMPERATU

RE

RA

NG

E

-200°

90°

*






4ti-0581 Rollof1000perforatedsheets125mmx78mm







www.4ti.co.uk | 67 +44 (0)1306 884885

Hea

t Se

alin

g

Pierce Seal

PEELABLE

ThislaminatematerialgivesapeelableheatsealtopolypropyleneandCOCplates.Itcanbepeeleddirectlyfromtheplateimmediatelyafterremovalfromthefreezer.TheDMSOresistantpeelsealisanexcellentbarriertoaqueoussolutionsandsolvents,inparticularDMSO,evenatroomtemperature.

Itformsaweld-sealtopolyethyleneplates(cannotbepeeled),requiringabladeorneedleforaccess.

DMSO Resistant Peel Seal

Applications

• LowandroomtemperaturecompoundstoragewithDMSO*andotherorganicsolvents

• 100%DMSOcanbestoredatroomtemperaturefor12monthswithnodeteriorationofthesealquality

• Availableinsheetandrollformatforautomatedsealers


Thisfoil-basedheatsealissuitablefortopolypropyleneandpolystyreneplatesandcanbeeasilypiercedwithamanualorautopipettetip.Ithasverygoodsolventresistance,includingtoDMSO.Sealscanbeappliedontopofeachotherallowingmultipleaccess/resealingofsamples.Abluecolourprintidentifiesthenon-sealingsurfaceforeaseofapplication.Thissealremainsintacttoveryhightemperatures.

Applications

• PCR

• LowandroomtemperaturecompoundstoragewithDMSOandotherorganicsolvents

• Sampleshipping



SE

AL

INT

EG

R

ITY TEMPERATU

RE

RA

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-80°

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A

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PIERCEABLE

SE

AL

INT

EG

R

ITY TEMPERATU

RE

RA

NG

E

-20°

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4ti-0530S Trialroll1



4ti-0532S Trialroll2


| www.4ti.co.uk68 +44 (0)1306 884885

Heat Sealing

TheINDISealisafoil-basedheatsealthatsealstopolypropyleneandissuitableforlowtemperaturestorage.Thesealconsistsof96perforatedindividualsealingcapsinafoilframe.Theframeisremovedafterheatsealingleavingeachwellwithanindividualairtightseal.Thesealshavetabsforeasyremovalbyhandorwithforceps,withoutleavinganyresidue.

Mainlydevelopedforusewithtubesinclusterrackssomeplatescanalsobesealed.

Re-sealingandpiercingarepossible.

96 INDI™ Seal

Applications

• Compoundstorage

• Sampleshipping


AnaluminiumheatsealthatsealstoPPandPSplatesandcanbepiercedwithapipettetiporpeeledbyhand.GoodsolventresistanceincludingDMSO.Resealingontoanexistingsealispossible.Aredcolourprintidentifiesthenon-sealingsurfaceforeaseofapplication.

Foil Seal

Applications

• Lowtemperaturecompoundstorage

• Short-termroomtemperaturecompoundstorage

• PCR

• Resealable



PEELABLE

A

UTOCLAVABLE

121o

PIERCEABLE

SE

AL

INT

EG

R

ITY TEMPERATU

RE

RA

NG

E

-0°

110°

PIERCEABLEPEELABLE

SE

AL

INT

EG

R

ITY TEMPERATU

RE

RA

NG

E

-195°

120°


4ti-0543 INDISealforindividualtubesealing

(125mmx78mmsheets) 50sheets








4ti-0536 100sheets(125mmx78mm)1 Compatible with Thermo Fisher ALPS 300™ / ALPS 3000™ /

KBiosystems Wasp™ / KBioscience FlexiSeal and Cube2 Compatible with Agilent (Velocity 11) PlateLoc®

3 Compatible with REMP Portrait Heat Sealer (PHS)4 Compatible with REMP Landscape / Stacking Heat

Sealers (LHS/SHS)


www.4ti.co.uk | 69 +44 (0)1306 884885

Hea

t Se

alin

g

Thisisaheavydutylaminatematerialthatsealstopolypropylenetogiveaverystrongbutpeelableseal.Thissealaffordsahighdegreeofsampleprotection.GoodsolventresistanceincludingDMSO.

Thermal Bond

Applications

• Longtermstorage,includingstorageandtransportationatlowtemperature

• Storageoforganicsolvents,acidsandalkalinesincludingDMSO

• PCR

• Suitableforwaterbaththermalcyclers

• Availableinsheetandrollformatsforautomatedsealers


A

UTOCLAVABLE

121o

PEELABLE

SE

AL

INT

EG

R

ITY TEMPERATU

RE

RA

NG

E

-200°

110°

Thepolystyrenefoilsealcreatesastrong,butpeelablesealtopolystyreneplates.Thesealcanalsobepiercedwithapipettetipandhasagoodsolventresistance,includingtoDMSO.Resealingontoanexistingsealispossible.

Polystyrene Foil Seal

Applications

• Lowtemperaturecompoundstorage

• Short-termroomtemperaturecompoundstorage

• Resealingispossible




PIERCEABLEPEELABLE

SE

AL

INT

EG

R

ITY TEMPERATU

RE

RA

NG

E

-20°

110°





4ti-0591 100sheets(125mmx78mm)5mx78mm

| www.4ti.co.uk70 +44 (0)1306 884885

Heat Sealing

Thisisafoil-basedheatsealwhichiscoatedonthesealingsurfacewithalowautofluorescenceblackpigmentforusewithlightsensitivesamplesorforfluorescentassays.TheBlackSealformsapeelablesealtopolystyreneplates.

Black Seal

Applications

• Fluorescentandotherlightsensitiveassays.

• Pierceswithaforcegreaterthan10N(ASTMD1000)

• Availableinsheetandrollformatsforautomatedsealers


1 Compatible with Thermo Fisher ALPS 300™ / ALPS 3000™

/ KBiosystems Wasp™ / KBioscience FlexiSeal and Cube2 Compatible with Agilent (Velocity 11) PlateLoc®





4ti-0527 100sheets(125mmx78mm

PEELABLE

SE

AL

INT

EG

R

ITY TEMPERATU

RE

RA

NG

E

-20°

80°

PIERCEABLE

ThisheatsealhasasmallerporesizethanthediscontiunedGasPermeableSeal(4ti-0596)whichstillallowsforgaseousexchange,butlimitsevaporation.Handlinginautomatedsealersisimproved.TheGasPermeableHeatSealMk2issuitableforcellcultureandisavailableassterileproductforthisapplication.

Gas Permeable Seal Mk 2

Applications

• Cellculture

• Overnightincubation

• Seedandinsectstorage

• Permeabilityrateis>20g/m2/24h

• Poresizeis<20µm

• Availableinsheetandrollformatforautomatedheatsealers


A

UTOCLAVABLE121o

PEELABLE

PIERCEABLESTERILE

SE

AL

INT

EG

R

ITY TEMPERATU

RE

RA

NG

E-20°

80°


4ti-0598 GasPermeableSealMk2roll(610mx78mm)1Approx5000seals 1roll

4ti-0598/ST GasPermeableSealMk2roll,sterile(610mx78mm)1

Approx5000seals 1roll

4ti-0599 GasPermeableSealMk2roll(500mx115mm)2Approx6200seals 1roll

4ti-0599/ST GasPermeableSealMk2roll,sterile(500mx115mm)2

Approx6200seals 1roll

4ti-0597 GasPermeableSealMk2sheets(125mmx78mm) 100sheets

4ti-0597/ST GasPermeableSealMk2sheets,sterile(125mmx78mm) 100sheets

(10packsx10sheets)

www.4ti.co.uk | 71 +44 (0)1306 884885

Hea

t Se

alin

g

Gas Permeable Clear Seal

Applications

• Insectstudies

• Seedstorage



PEELABLE

SE

AL

INT

EG

R

ITY TEMPERATU

RE

RA

NG

E

-80°

110°

Thisisaclearpolymerfilmformingapeelablesealtopolypropylene,polyethylene,polystyreneandCOCplateswithgoodopticalclarityandmoderatesolventresistance.Thefilmhas3mmslitsacrosstheentirewidthoftherollorsheet,allowinggaseousexchange.DuetoitswidertemperaturetolerancecomparedtotheGasPermeableHeatSealitcanbeusedforstoragee.g.forseedsorinsects.


4ti-0540/SLIT 480mx78mmroll1Approx4000seals

4ti-0541/SLIT 100sheets(125mmx78mm)

1 Compatible with Thermo Fisher ALPS 300™ / ALPS 3000™

/ KBiosystems Wasp™ / KBioscience FlexiSeal and Cube2 Compatible with Agilent (Velocity 11) PlateLoc®


| www.4ti.co.uk72 +44 (0)1306 884885

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www.4ti.co.uk | 73 +44 (0)1306 884885

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ti.c

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Heat Seal Selection Table

| www.4ti.co.uk74 +44 (0)1306 884885

Code Description Qty

4ti-0575/FS ClearWeldSealMkIIFrameSealTM,stackableheatsealframes 50

4ti-0541/FS ClearSealFrameSealTM 50

4ti-0531/FS PierceSealFrameSealTM 50COMING SOON

FrameSealTMutilisesasheetofstandard4titude®heatsealmaterialmountedontoadisposable,rigid,plasticframe.AFrameSealTMcanbeplacedeithermanuallyorwitharoboticgripperontoawiderangeofskirtedandsemi-skirtedmicroplatesincludingPCRplatesandarecompatiblewiththe4S3heatsealer.Aftersealing,thereistheoptiontoremovetheframebytearingalongpre-cutlinesleavingtheheatsealinplaceontheplatesurface.

FrameSeal™

Application example:

OurClearWeldSealMkII(4ti-0575)heatsealingfilmisnowavailablepre-mountedtocreateaFrameSeal.TogetherwithFraunhofferIPAInstituteFrameSealTMhasbeenusedinafullyintegratedsolutionforsealing1536wellPCRplatesbeforeanalysisofRNAiscreeningwithaRocheLightcycler1536.

Usingaroboticarm,asingleFrameSealTMisplacedontoa1536wellPCRplate.Byadjustingthepositionoftherobotgripper,theplateandFrameSealTMcombinationarethentransferredtothedrawofa4S3HeatSealerwhichsealsthepartviaasoftwarecommandthroughitsRS232port.

Afterthesealingprocesshasbeencarriedout,thesealedplateistransferredtotheDe-Framer,whichseperatestheframefromthefilmateachpointalongtheedgeofthefilm.Theframecanthenberemovedleavingthesealedplatetobeplacedinthecycler.

TogethertheheatsealerandtheFrameSealTMpresentedhereare:

• Themostcosteffectiveautomatedheatsealingsolutionavailable

• Anapproachinlinewithdemandsofdiagnosticsforasterile,single-useconsumable

• Themostcompactinstrumentforintegration

4s3™shownnexttotheDe-Framer

Automationcell,showingthe4LAB™,4s3™andDe-Framerfullyintegrated

Key advantages

• Keyadvantagescomparedtosheetsofsealingfilmorrollmaterial,aretheeaseofhandlingandtheabilitytosteriliseandimprovethequalitycontrolofeverysingleseal.

• TheFrameSealTMalsoenablestheuseofsheetedsealingmaterialinautomation.

NEW

COMING SOON


AdhesiveSealing

Adhesivesealsprovideaversatile,yetrobust,methodforprotectingsamples.Thesealsaresuppliedassheetsandsomearealsoavailableinrollformat.Mostadhesivesealsareprovidedwithendtabsforeasyapplicationandremoval.

AlloursealsareproducedandprocessedunderstrictlycontrolledenvironmentalconditionsandcertifiedfreefromDNase,RNaseandhumangenomicDNA.

Tochoosethemostsuitablesealpleaserefertocomparisontablepage83whichdescribestherecommendedapplicationsandtechnicalfeaturesofeachseal.

Toobtainthebestsealingresultswithadhesivesealswestronglyrecommendedtheuseofhighqualityplateslikeourswithraisedrimsealingringsforoptimalsealingintegrityandguaranteedflatness.Toimprovesealapplicationweoffersupplementaryproductslikethesealrollerpage53andthecompressionpadseepage52.

WehaveaddedthreenewsealstoourrangeformorespecialisedapplicationsincludinggaspermeablesealsandanInterSealTMideallysuitedforkitmanufacturers,p82.

4titude®offerawiderangeofsealsfromgeneralmicroplatecoverstospecialisedgaspermeablesealsforcellcultureapplications.


Adhesive Sealing

Applications

• PCR

• qPCRandotheropticalapplications

• lowtemperaturestorageandtransportation

• availableinsheetandrollformat


PCR Seal

For best results

For all adhesive seals, the best

sealing results are achieved using the

adhesive seal roller (4ti-0502).

For best results




PEELABLE

Applications

• SuitableforqPCRandotheropticalapplications

• Forusewith96-wellplatesonly


Q-Stick™ qPCR Seal



PEELABLE

ThePCRsealisadurabletransparentpolyesterfilmwithastrongadhesivelayer.Thissealenablesahighsealintegrityandefficientlypreventssampleevaporation.ItisrecommendedforPCR,qPCRandotheropticalapplications(e.g.fluorescenceorcolorimetricmeasurements)aswellassamplestorage.

Adhesivefreeendtabsfacilitateapplicationandremoval.Thesealscanbeeasilypeeledfromtheplatewithoutleavinganystickyresidue.

* (with pressurised heated PCR lid)

SE

AL

INT

EG

R

ITY TEMPERATU

RE

RA

NG

E

-20°

110°

*


4ti-0500 100 sheets (135mm x 80mm)

4ti-0501 1 laminated roll (100m x 80mm) Approx 700 seals

ThisuniquesealcombinesthestrongsealingintegrityofourPCRsealwithimprovedopticalpropertiesthanksto96adhesive-freewindows.ItisrecommendedforqPCRandotheropticalapplications(e.g.fluorescenceorcolorimetricmeasurements).

Theuseofadhesivefreeendtabsenableseasyalignmentofthewindowsona96wellplateaswellaseasyremovaloftheseal.

* (with pressurised heated PCR lid)

SE

AL

INT

EG

R

ITY TEMPERATU

RE

RA

NG

E

-20°

110°

*

www.4ti.co.uk | 77 +44 (0)1306 884885

Adh

esiv

e Se

alin

g

For best results




For best results




• Noadhesivetocomeintocontactwithsamples

• EnhancedopticalclaritysuitableforqPCR

• Suitableforallplatetypes

• Peelablewithoutleavingstickyresidueontheplatewhenthesealispeeledoff

• SuitableforqPCRandotheropticalapplications

• Availableinsheetandrollformat


qPCR Seal

Thisopticallyclearsealwasspecificallydevelopedforopticalapplications,particularilyqPCR.Itisnon-tackytoskinandglovesenablingeaseofhandlingandapplicationandpositioning.Thissealisapressureactivatedseal.Thepolyesterfilmhasadhesiveheldwithincapsulesontheunderside,andpressuremustbeappliedtothetop-side,aroundtheraised-wellrimstoactivatetheadhesive.

PEELABLE

Applications

• PCRandotherhightemperatureapplications

• Samplestorage




PCR Foil Seal

PIERCEABLE PEELABLE

SE

AL

INT

EG

R

ITY TEMPERATU

RE

RA

NG

E

-40°

120°

*(with pressurised heated PCR lid)

SE

AL

INT

EG

R

ITY TEMPERATU

RE

RA

NG

E

-40°

110°

*



4ti-0561 1roll(100mx80mm)Approx700seals

Thisaluminiumfoilsealhadastrongacrylicadhesivewhichproducesasealofhighintegrity.ThissealwasdevelopedforPCRandotherhightemperatureapplicationsduetoitseffectivenessinpreventingsampleevaporation.ThePCRFoilSealisapiercableseal.Whenpierced,theirregularmannerinwhichthefoiltearspreventstheformationofavacuum.

Thesealhasperforatedendtabsforeasyapplicationandremovalbypeeling.




| www.4ti.co.uk78 +44 (0)1306 884885

Adhesive SealingApplications

• Suitablefor96wellplates(autosamplers,HPLC,DNAsequencers)

• Piercableforsampleretrieval


• Endtabsforeaseofapplicationandremoval


Pierceable Film*




PIERCEABLE PEELABLE

SE

AL

INT

EG

R

ITY TEMPERATU

RE

RA

NG

E

-20°

110°

Applications

• Eukaryoticcellculture,bacterialcultureandovernightincubations.

• MoistureVapourTransmissionRateis6200g/m2/day

• AirPorosityis15sec/100cc/in2



Gas Permeable Seal

STERILE

PIERCEABLE PEELABLE

SE

AL

INT

EG

R

ITY TEMPERATU

RE

RA

NG

E

-20°

80°

Thisadhesivesealisperforatedwithmicroporessothatitispermeabletogasesbutprovidesabarriertobacteriaandothersolidcontaminants.Thegaspermeablesealisaconvenientoptionforcoveringcellculturesandcanbepiercedorpeeledwithoutanyremainingstickyresidue.

Endtabsfacilitateeaseofapplicationandremoval.

Thisstrongadhesivesealwasdevelopedtofacilitateeasysampleremovalwithamanualorautomatedsystem.Thesealisatransparentpolyesterfilmwithastrongadhesivelayer.96adhesivefreewindowshavebeencross-cut.Thisenablesaccesstothesamplewellswithatiporprobewithminimalpressure.

SampleevaporationisminimizedwhenautosamplerssuchasHPLCandcapillarysequencersareused.

For best results




For best results






4ti-0515/ST 50sterilesheets(5packsof10sheets,135mmx80mm)

4ti-0515/R 1roll(100mx80mm)Approx700seals

*NotavailableintheUSA

www.4ti.co.uk | 79 +44 (0)1306 884885

Adh

esiv

e Se

alin

g

*NotavailableintheUSA

• Suitableforeukaryoticcellculture,bacterialcultureandlong-termliveassays

• Suitablefor96or384wellplates

• MoistureVapourTransmissionRateis1g/m2.d.bar

• AirPorosityis600cm3/m2.d.bar

• Peelableleavingnoresidue

• Endtabsforeaseofapplicationandremoval


Gas Permeable Moisture Barrier Seal

PEELABLE

STERILEPIERCEABLE

SE

AL

INT

EG

R

ITY TEMPERATU

RE

RA

NG

E

-20°

80°

Applications

• Suitableforbacterialorcellculture.

• MoistureVapourTransmissionRateis4200g/m2/day

• AirPorosityis10sec/100cc/in2

• Strongsolventresistance

• Availableinsheetorrollformat


Air-O-Seal

Air-O-Sealisahydrophobic,gaspermeable,adhesivesealwhichismadefromtapewithanacrylateadhesive.Air-O-SealhasamuchlowermoisturetransferratethanourstandardGasPermeableAdhesiveSeal(4ti-0515).Itisresistanttoacids,DMSO,detergents,ethanol,glycerol,ispropanol,sodiumhypochlorite(bleach)andsuitableforbacterialorcellculture.



4ti-0517S 100sterilesheets(10packsof10sheets,135mmx80mm)

4ti-0517R 1roll(150mx80mm)Approx1250seals

STERILEPEELABLE

SE

AL

INT

EG

R

ITY TEMPERATU

RE

RA

NG

E

-20°

30°

Thisisastrongadhesivesealthathas96or384adhesivefreewindows.ThesewindowsallowairandCO2exchange,butpreventmoistureevaporationfromthesample.

Thesealisprimarilyusedincellcultureforeukaryoticorbacterialcells.Itisultraclearforimagingwithouthavingtoremovetheseal.

For best results




For best results





4ti-0516/96 50sheets(5packsof10sheets,137mmx77mm),96round-wellonly

4ti-0516/384 50sheets(5packsof10sheets,137mmx77mm),384square-wellonly

NEW

| www.4ti.co.uk80 +44 (0)1306 884885

Adhesive SealingApplications

• Suitableformediumtolong-term(<5yrs)storageofsamplescontainingahighconcentration(<80%)ofDMSO.

• Strongadhesiveseal



Peelable Solvent Resistant Foil


4ti-0512 100sheet(122mmx80mm)

PEELABLE

SE

AL

INT

EG

R

ITY TEMPERATU

RE

RA

NG

E

-20°

80°

Applications

• Generalmicroplatecover

• Reducesshorttermsampleevaporation

• Eliminateriskofcontamination(Aqueoussamplesonly)


• Availableinsheetandrollformats


Microplate Seal

PEELABLE

SE

AL

INT

EG

R

ITY TEMPERATU

RE

RA

NG

E

-20°

80°

Thissealisbasedonanaluminiumfoilwithafilmlayerandsiliconeadhesive.Thislaminatehasaveryhighlevelofsolventresistanceandisrecommendedforlongtermsamplestorage.

Asingleendtabaidsapplicationandremovalbypeeling.

Thistransparentpolyesterbasedfilmhasamediumstrengthadhesive.Itisdesignedasalowcostsealingoptionandusefulfortemporarystorageandasacoverforapplicationssuchascentrifugation.

Endtabsallowforeasyapplicationandremoval.Thissealisremovablewithoutresidueontheplate.

For best results




For best results





4ti-0510 100sheet(130mmx80mm)

4ti-510/R 1roll(110mx80mm)Approx800seals

www.4ti.co.uk | 81 +44 (0)1306 884885

Adh

esiv

e Se

alin

g

Applications

• Bacterialculture

• Cellculture

• Moisturevapourtransmissionrateof450gms/m2/day



Applications

• Idealforkitmanufacturerstoallowflexibleaccesstoindividualwells

• ForusewithVariStrips(inaframe)orBreak-A-Wayplates

• Re-sealingwithouttheneedforaheatsealer

• Endtabsforeasyapplicationandremoval

Double Skin Breathable Film

InterSeal


4ti-0518 100sheets(135x83mm)


4ti-0519 100sheets(148mmx98mmincludingliner)

PEELABLE

PIERCEABLE PEELABLE

SE

AL

INT

EG

R

ITY TEMPERATU

RE

RA

NG

E

0°

40°

SE

AL

INT

EG

R

ITY TEMPERATU

RE

RA

NG

E

-20°

110°

Thissealisanultrathinopaquemembranewhichisgaspermeablewhilstprovidinganeffectivebarriertomoisture.AirandCO2exchangeispossible,butevaporationisprevented.

Endtabsaidapplicationofthissealanditsremovalbypeeling.

Adoublesidedblackadhesivemicroplatefilmtofacilitatethesealing,accessing(piercing)andresealingofVariStrips(4ti-0753)priortoPCR.

Thestripsorplatesarefilledwithreagentandsealedwith4ti-0530(PierceSeal).TheIntersealisoverlaidandthestripsorplatesprocessedwiththePlateCutter4ti-3000intoindividuallysealedstrips.ThestripscanthenbetransportedandopenedatacustomersitebypeelingtheprotectedlineroftheInterSealoffandaccessingthesamplethroughthepierceseal.Thestripscanthenbesealedwithafoilorfilmwhichisappliedtotheexposedblackadhesivewithouttheneedforaheatsealer.

Also see

Variplates tm (page 42-43)

plate cutter (page 53)

NEW

NEW

| www.4ti.co.uk82 +44 (0)1306 884885

Adhesive Seal C

omparison Table

PC

R S

ea

l

p7

7

Q-S

tick™ fo

r q

PC

R

p7

7

qP

CR

Se

al

p78

PC

R Fo

il Se

al

p78

Pie

rcab

le F

ilm

p7

9

Ga

s Pe

rme

ab

le

Se

al

p7

9

Ga

s Pe

rme

ab

le

Mo

isture

B

arrie

r Se

al

p8

0

Air-O

-Se

al

p8

0

Pe

ela

ble

S

olve

nt

Re

sistan

t Foil

p8

1

Micro

pla

te S

ea

l

p8

1

Do

ub

le S

kin

Bre

ath

ab

le F

ilm

p8

2

Inte

rSe

al

p8

2

Ap

plica

tion

PC

Rq

PC

R,

fluo

resce

nce.

96

-we

ll m

icrop

late

s o

nly

qP

CR

& o

the

r flu

ore

scen

t a

pp

licatio

ns

PC

R &

sam

ple

sto

rag

eS

am

ple

a

pp

licatio

n

or re

trieval.

96

-we

ll pla

tes

on

ly.

Ce

ll & b

acte

rial

cultu

reE

uka

ryotic

cell cu

lture,

ba

cteria

l

cultu

re a

nd

lo

ng

-term

live

assays.

Ba

cteria

l or ce

ll cu

lture

Micro

pla

te

sea

ling

co

nta

inin

g

solve

nts in

cl. D

MS

O

Aq

ue

ou

s sa

mp

le sto

rag

eB

acte

ria cu

lture

Dia

gn

ostic kit

pro

du

ction

Sp

ecia

l Pro

pe

rties

Go

od

op

tical

clarity

Discre

et o

ptica

l w

ind

ow

s for 9

6-

we

ll pla

tes

Go

od

op

tical

clarity

Irreg

ula

r te

arin

g w

he

n

pie

rced

p

reven

ts fo

rma

tion

of

vacu

um

Pre

-cut w

ith

cross w

hich

re

du

ces tip

or

pro

be

be

com

ing

clo

gg

ed

Ga

s pe

rme

ab

le

pro

vidin

g

ba

rrier to

b

acte

ria &

o

the

r solid

co

nta

min

an

ts

Ga

s pe

rme

ab

le

tha

t allo

ws a

ir a

nd

CO

2

excha

ng

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ut p

reven

ts m

oistu

re

evap

ora

tion

.

Air-O

-Se

al h

as a

mu

ch lo

we

r

mo

isture

tra

nsfe

r rate

th

an

ou

r Ga

s P

erm

ea

ble

A

dh

esive

Se

al

(4ti-0

515

) an

d

is suita

ble

for

ba

cteria

l or ce

ll cu

lture.

Hig

h so

lven

t re

sistan

ceM

ed

ium

stre

ng

th

tran

spa

ren

t se

al

Th

in g

as

pe

rme

ab

le, m

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re

ba

rrier se

al.

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ws a

ir an

d

CO

2

excha

ng

e

bu

t preve

nts

evap

ora

tion

.

2 se

alin

g

surfa

ces

Op

tical w

ind

ow

s

Min

Tem

p-2

0°C

-20

°C-8

0°C

-40

°C-8

0°C

-20

°C-2

0°C

-20

°C-2

0°C

-20

°C0

°C-2

0°C

Ma

x Tem

p110

°C110

°C110

°C12

0°C

110°C

80

°C8

0°C

30

°C8

0°C

80

°C4

0°C

110°C

Ste

rile•

•

Pie

rcab

le•

••

••

Pe

ela

ble

••

••

••

••

••

••

RN

ase

/ Dn

ase

free

••

••

••

••

••

••

Me

asu

rem

en

ts

(mm

)*

135

mm

x 80

mm

133

mm

x 76m

m14

0m

m x 7

7m

m13

0m

m x 8

0m

m13

5m

m x 7

7m

m13

5m

m x 8

0m

m13

7m

m x 7

7m

m13

5m

m x 8

0m

m12

2m

m x 8

0m

m13

0m

m x 8

0m

m13

5m

m x 8

3m

m14

8m

m x 9

8m

m

100

she

ets

4ti-0

50

04

ti-05

65

4ti-0

56

04

ti-05

50

4ti-0

56

64

ti-05

154

ti-05

164

ti-05

174

ti-05

124

ti-05

104

ti-05

18

4ti-0

519

www.4ti.co.uk | 83 +44 (0)1306 884885

Date post:	11-May-2021
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