Selection and UtilizationSelection and Utilizationof of MinipigsMinipigs for Biomedical Researchfor Biomedical Research
Mr. Hsien-Pin ChuTaitung Animal Propagation Station, Livestock Research Institute
Lanyu pig
The local breed usually has a Smaller body sizeSlower growth rate Less lean meat contentadapting characteristics:heat tolerancedisease-resistanceroughage toleranceearly maturity
Agriculture biodiversity
Origin
Development of experimental mini-pig
Introduction policy made by government
Not found in main Island. Finally found in Lanyu islet.
Germplasm Introduction1
3, NTU, 1975
416, TLRI, 1980
Prof. Tang-yuan Lee,Prof. Yuang-yi SungDepartment of Animal Husbandry,National Taiwan University.Searching for indigenous mini-pig1975
Lanyu islet
The aboriginal tribe: Tao
416, 1980
1
3, 1975
Missions of minipig (TLRI)
Conservation
Utilization
Preserve agricultural
biodiversity
Maintain genetic
diversity
Basic investigationsBreeding plansExtension
Experimental animal
Lanyu pigGermplasm
The basic conservation herd was established in 1980.
4 males and 16 females
Gerplasm Collection
The random mating population was appointed as national germplasm conservation herd by Council of Agriculture since 1987. 15 males and 45 females
Gerplasm Maintenance
1980Introduction
(416)
Binlang pig,(Lanyu 400,2011)
2001
1989 Lanyu
Duroc
Mitsai pig(Lanyu 50, 2003)
1993Spotty coat color
selection
Spotty Lanyu pig(Lanyu 100,2003)
Lanyu pig GPI-CRC- PGD homologue
genotype line(Lanyu 300, 2008)
GPI-CRC-PGDhomologue selection
Lanyu pig conservation line(Lanyu 200,2008)
199250% Lanyu,50% Duroc
White coat colorselection
Minipig Breeds of TLRI Selection and Registration
Breed Breed code Genetic composition
Lanyu pig GPI-CRC-PGD homologue genotype line Lanyu 300Lanyu 300 100% Lanyu100% Lanyu
Spotty Lanyu pig Lanyu 100Lanyu 100 100% Lanyu100% Lanyu
Binlang pig Lanyu 400Lanyu 400 100% Lanyu100% Lanyu
Mitsai pig Lanyu 50Lanyu 50 50% Lanyu50% Lanyu50% Duroc50% Duroc
20082008
20032003
20102010
20032003
Breed Basic information
Lanyu pig GPILanyu pig GPI--CRCCRC--PGD PGD homologue genotype linehomologue genotype line
weight at birthweight at birth0.6~0.8 kg0.6~0.8 kg
weight at sexual maturityweight at sexual maturity20~25 kg20~25 kg
Sexual maturitySexual maturity3~4 months3~4 months
Spotty Lanyu pigSpotty Lanyu pig
Binlang pigBinlang pig
Mitsai pigMitsai pig
weight at birthweight at birth0.7~0.9 kg0.7~0.9 kg
weight at sexual maturityweight at sexual maturity27~33 kg27~33 kg
Sexual maturitySexual maturity5 months5 months
Reproductive parameters of swine, and miniature swine with the adultweights of Micropig and Minipig.
Parameter SwineMiniature swine
Micropig MinipigWeight at birth (kg) 1.30 0.45~0.60 0.60~1.00Weight at sexual maturity (kg) 90~120 15~18 25~30Weight at body maturity (kg) 200~300 35~55 70~90Sexual maturity (month) 6 4~5 6
Estrus cycle (day) 14 14 14
Length of estrus (day) 3 3 3
Length of pregnancy (day) 114 114 114
Litter size 10~14 5~8 5~8
Age at weaning (day) 28~35 28~35 28~35aYucatan micropig, Gottingen and Sinclair minipigbYucatan and Hanford minipig
The Laboratory Swine,2000 by Peter Bollen PJA; AK Hansen & HJ Rasmussen. CRC Press.
Lanyu minipig breeds
Conservation line Maintain genetic diversity
homologue genotype line Increase genetic homozygasityGPI-CRC-PGD
ConservationlineConservationline MaintaingeneticdiversityMaintaingeneticdiversity
Genetics MonitoringThe genetic variation and genetic distance in each individual of the conservation were monitored each generation by molecular biological techniques.
Population managementThe well-established genetic relationship of Lanyu pigs could be applied for maintaining genetic diversity of the conservation herd.
Gene Chromosome Genotype Function
GPI 6th BB Glucose phosphate isomerase
CRC 6th CC Calcium Release Channel
PGD 6th AA 6-Phosphogluconate dehydrogenase
LanyupigGPILanyupigGPICRCCRCPGDhomologuesgenotypelinePGDhomologuesgenotypeline
registered as a new breed in 2008.
CRC-CC Type (Normal type)free of stress-shock syndrome
and suitable to be laboratory animal.
Breeding history of Spotty Lanyu pig
Breeding history of Spotty Lanyu pig
E EP
E
EP
E E E EP
E EP EPEP
Under random mating system
E EP
E EP
E dominance, black
EPblack spotting
e recessive, red
E E E EP
Breeding history of Spotty Lanyu pig
EPEP
EP EP
EP
EP
Isolated breeding
EPEPEPEP
EPEP EPEP
EPEP
Breeding history of Spotty Lanyu pig
New breed from hybrid
50Duroc50Lanyu
Breeding history of Mistai pig
Coat color with its frequency()
0001000
F6
08.100
F5
04.41.721.1
F4
031.81.441.5
F3
018.043.531.0
F2
26.201.30
F1
72.5
7.5
25.3
72.8
91.9
Brown-black strips
Brown-white stripes
Solid black Solid Brown With spots
Breeding history of Mistai pig
28
BinlangpigBinlangpig
Binlang Village, Beinan Township, Taitung County, Taiwan
29
Breeding history of Binlang pig
E EP
E EP
E
EP
E E E EP
E EP EP EP
E EP
Breeding history of Binlang pig
EP EP
EP EP
EP EP
EP
EP
EP EPEP EP
EP EP EP EP
Breeding history of Binlang pig
Ep
e
EpEp
Ep,e
Ep e
Ep,e e,e
Ep,e
Ep,e
Breeding history of Binlang pig
e,e
e
e
e e
e,e
e,e
e,e e,e
e,e
Breeding history of Binlang pig
Extension for biomedical research
2004/9/20
Holding symposium
2009/9/22
Holding discussion meeting
Holding workshop of Operative technique
2010/8/21~22
Holding international conference Minipig for Biomedical Research--
38
RFID
tag & reader
The system was based on 134.2 KHz RFID piglet ear tag, RFID reader, wireless
mass data collection device and wireless internet access environment.
40
http://minipigs.angrin.tlri.gov.tw/
41
42
Figure 1. Number of Minipig (TLRI) used for laboratory animals from 2004 to 2010
Category No. Item User*
Surgery 524
Medical deviceUrological technologyLaparoscopeStem cell technologyNew Drug DevelopmentAllotransplantationRegenerationTeaching testCardiology researchOperation technology
NCKUH, MMH, NTUCM, TVGM, FEMH, CGMH, KMUH, TSGH,MMH, CSMUH,NDMC
Dentistry 56 Regeneration NTUVM, TMUH, TVGM
Emergency 78 TrainingOperation test FAST, NTUVM, NTUCM
Internal Medicine 228
Cardiac vascular checkdiabetes therapyCancer therapyCardio cell regenerationNew Drug Development
CGMH, NTUCM, NCKUH,CGMH,NTUVM
Anesthesiology 96 Teaching test MMH, CMMC,NDM
Orthopedics 82 AllotransplantationRegeneration CGMH, CMUH, NTUVM
Radiology 35 Medical image NHRI
Transgenic engineering 23 New Drug Development ATIT
Pharmacology 10 Teaching test YCHM
TotalTotal 11321132 37 projects37 projects 17 users17 users
Table 1. Utilization of minipigs for biomedical research from 2005 to 2010
Table 1. (continued)
NDMC: National Defense Medical CenterNTUH: National Taiwan University HospitalNCKUH: National Cheng Kung University hospital,KCGMH: Kaohsiung Chang-Gung Memorial Hospital,KMUH: Kaohsiung Medical University hospital,NTUVM: School of Veterinary Medicine NTU,TMMH: Taipei Mackay Memorial Hospital,CMMC: Chi Mei Medical Center,NHRI: National Health Research Institutes,TMUH: Taipei Medical University & Hospital,TVGH: Taichung Veterans General Hospital,CMUH: China Medical University Hospital,CSMUH: Chung Shan medical University Hospital,FAST: Formosa Association for the Surgery of Trauma,YCHM: Yuh-Ing Junior College of Health Care & Management,ATIT: Animal Technology Institute Taiwan.
*:
46
Journal of Surgical Research 153: 268-237, 2009 (SCI; IF.=1.875, Surgery 49/148)
Kaohsiung ChangKaohsiung Chang--Gung Memorial HospitalGung Memorial Hospital
Team Team
YurYur--Ren Kuo, M.D. Ph.D. Ren Kuo, M.D. Ph.D. et al.et al.
Swine hemi-facial composite tissue allotransplantation: A model to study immune rejection.
47
Mesenchymal stem cells prolong composite tissue allotransplant survival in a swine model.
Transplantation 87: 1769-1777, 2009. (SCI; IF=3.816, Surgery 10/148)
Kaohsiung ChangKaohsiung Chang--Gung Memorial HospitalGung Memorial Hospital
Team Team
YurYur--Ren Kuo, M.D. Ph.D. Ren Kuo, M.D. Ph.D. et al.et al.
Intramyocardial Peptide Nanofiber Injection Improves Postinfarction Ventricular Remodeling and Efficacy of Bone
Marrow Cell Therapy in Pigs
Circulation 2010;122;S132-S141
National Cheng Kung University hospital,National Cheng Kung University hospital,
Team Team
Patrick C.H. Hsieh, MD, PhDPatrick C.H. H
