SelexION® and SelexION®+ Technology Site Planning Guide

SelexION® and SelexION®+ Technology

for 5500, 6500, and 6500+ Series SystemsSite Planning Guide

December 2015RUO-IDV-01-1831-C | D5024211 E

This document is provided to customers who have purchased SCIEX equipment to use in the operation of such SCIEXequipment. This document is copyright protected and any reproduction of this document or any part of this document isstrictly prohibited, except as SCIEX may authorize in writing.

Software that may be described in this document is furnished under a license agreement. It is against the law to copy, modify,or distribute the software on any medium, except as specifically allowed in the license agreement. Furthermore, the licenseagreement may prohibit the software from being disassembled, reverse engineered, or decompiled for any purpose. Warrantiesare as stated therein.

Portions of this document may make reference to other manufacturers and/or their products, which may contain parts whosenames are registered as trademarks and/or function as trademarks of their respective owners. Any such use is intended onlyto designate those manufacturers' products as supplied by SCIEX for incorporation into its equipment and does not implyany right and/or license to use or permit others to use such manufacturers' and/or their product names as trademarks.

SCIEX warranties are limited to those express warranties provided at the time of sale or license of its products and are SCIEX’ssole and exclusive representations, warranties, and obligations. SCIEX makes no other warranty of any kind whatsoever,expressed or implied, including without limitation, warranties of merchantability or fitness for a particular purpose, whetherarising from a statute or otherwise in law or from a course of dealing or usage of trade, all of which are expressly disclaimed,and assumes no responsibility or contingent liability, including indirect or consequential damages, for any use by the purchaseror for any adverse circumstances arising therefrom.

For research use only. Not for use in diagnostic procedures.

AB Sciex is doing business as SCIEX.

The trademarks mentioned herein are the property of AB Sciex Pte. Ltd. or their respective owners.

AB SCIEX™ is being used under license.

© 2015 AB Sciex

AB Sciex Pte. Ltd.Blk 33, #04-06Marsiling Ind Estate Road 3Woodlands Central Indus. Estate.SINGAPORE 739256

Site Planning GuideSelexION® and SelexION®+Technology

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Chapter 1 Introduction...............................................................................................................................4Customer Site Planner Responsibilities..............................................................................................................................4FSE Responsibilities............................................................................................................................................................5

During Installation.......................................................................................................................................................5Operator Familiarization..............................................................................................................................................5

Technical Support..............................................................................................................................................................5

Chapter 2 Site Planning Checklist..............................................................................................................6Customer Information........................................................................................................................................................6Requirements.....................................................................................................................................................................6

Site Layout Requirements............................................................................................................................................6Electrical Requirements................................................................................................................................................7Gas Supply Requirements............................................................................................................................................9Ventilation and Waste-Collection Requirements........................................................................................................10Environmental Requirements.....................................................................................................................................10Solutions and Equipment Requirements....................................................................................................................11Product Familiarization..............................................................................................................................................11

Comments and Exceptions...............................................................................................................................................12Signoff.............................................................................................................................................................................12

Appendix A Site Requirements................................................................................................................13Site Layout Requirements................................................................................................................................................13

Weights and Dimensions...........................................................................................................................................13Installation Site Clearances........................................................................................................................................13

Electrical Requirements....................................................................................................................................................14Mains Supply Connections.........................................................................................................................................14Mains Supply Fluctuations.........................................................................................................................................14Protective Earth Conductor........................................................................................................................................14Uninterruptible Power Supply or Power Conditioner..................................................................................................15System Electrical Specifications..................................................................................................................................15

Gas Supply Requirements................................................................................................................................................16Optional Gas Generators...........................................................................................................................................18

Ventilation and Waste Collection Requirements..............................................................................................................19Environmental Requirements...........................................................................................................................................20

BioSafety Requirements.............................................................................................................................................21Sound Pressure Level.................................................................................................................................................21Vibration....................................................................................................................................................................22

Customer-Supplied Solutions and Equipment..................................................................................................................22

Appendix B Equipment Safety Categories..............................................................................................24

Revision History........................................................................................................................................25

SelexION® and SelexION®+Technology

Site Planning Guide

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Contents

This guide is for the site planner, the individual responsible for preparing the facility for the installation of theSelexION

® differential mobility separation technology device for 5500 and 6500 series instruments or the SelexION

®+

differential mobility separation technology for 6500+ series instruments.

The information in this guide supplements the information contained in the Site Planning Guide for the massspectrometer. The laboratory must meet the conditions detailed in both the mass spectrometer Site PlanningGuide and this Site Planning Guide before the Field Service Employee (FSE) can install the SelexION

® or

SelexION®+ technology.

Customer Site Planner ResponsibilitiesComplete the Site Planning Checklist on page 6, in consultation with facilities services personnel (gas,electrical, ventilation, and information technology [IT]), and return it to the FSE before the completion date. Referto Signoff on page 12.

Note: The FSE will follow up if the checklist is not received prior to the scheduled installation date.

• Verify that adequate space and the required shipping facilities are available. Refer to Site LayoutRequirements on page 13.

• Provide all required electrical receptacles. Refer to Electrical Requirements on page 14.

• Provide all required regulators, shut off valves, and gases. Refer to Gas Supply Requirements on page16.

• Provide and install all required vents and ventilation devices. Refer to Ventilation and Waste CollectionRequirements on page 19.

• Verify that the requirements for the operating environment are met. Refer to Environmental Requirementson page 20.

• Provide all required solutions and laboratory equipment, including all fittings, and sample tubing for the liquidchromatography (LC) equipment, unless purchased from SCIEX. Refer to Customer-Supplied Solutionsand Equipment on page 22.

• When the shipment arrives, inspect the packaging for damage and then contact SCIEX Customer Service orthe local FSE to schedule the installation.


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1Introduction

FSE Responsibilities• Review the checklist and discuss outstanding issues with the site planner.

• Test and qualify the system to the specifications in the Installation Checklist and Data Log.

During InstallationThe FSE unpacks the system (with the assistance of customer staff), sets up the system, and then confirms itsoperation. When the system reaches proper vacuum, the FSE conducts system performance tests.

Operator FamiliarizationDuring installation, the FSE provides a system and software overview, reviews data, and provides some basicoperator familiarization.

Note: Online training is available at sciex.com/education .

Technical SupportSCIEX and its representatives maintain a staff of fully-trained service and technical specialists located throughoutthe world. They can answer questions about the system or any technical issues that might arise. For moreinformation, visit the Web site at sciex.com.


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Introduction

#

#

Customer Information

Organization

Address

City

Country

Zip/Postal codeTelephone

FaxSite planner contactname

E-mail address

Requirements

Site Layout RequirementsRefer to Site Layout Requirements on page 13.

CompleteRequirement

The measured building clearances can accommodate the equipment and crate dimensions.

If the requirements cannot be met, then contact the FSE.


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2Site Planning Checklist

Electrical RequirementsRefer to Electrical Requirements on page 14.

N/ACompleteRequirement

Installation of electrical supplies and fixtures complies with local regulations andsafety standards.

One branch circuit is provided for the SelexION® differential mobility separation

technology device for 5500 and 6500 series instruments or the SelexION®+

differential mobility separation technology for 6500+ series instruments. It canbe connected to the same branch circuit as the acquisition computer.

The mains supply voltage does not fluctuate more than ±10% from the nominalvoltage.

The mains supply includes a correctly installed protective earth conductor.

(Optional) A customer-supplied UPS or power conditioner is provided for thesystem. The UPS or power conditioner must deliver 230 VAC, 50 Hz or 60 Hz,4000 VA (minimum) for 5500 systems, or 5000 VA (minimum) for 6500 and 6500+

systems. Refer to Uninterruptible Power Supply or Power Conditioneron page 15.

Note: SCIEX sells and supports a number of UPS power protection units formass spectrometer systems that are custom configured to provide a total backuppower solution. Contact an SCIEX sales representative for more information.

A qualified electrician has determined the appropriate AC mains supplyconfiguration based on the system electrical specifications found in SystemElectrical Specifications on page 15.


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Site Planning Checklist

Electrical Requirements (International)

Refer to Electrical Requirements on page 14.


Electrical installations use locally approved standard connections and cables.

Electrical Requirements (North America)

Refer to Electrical Requirements on page 14.


The branch circuit for the SelexION® or SelexION

®+ technology is 15 A, 200 VACto 240 VAC (208 VAC typical), 50 Hz or 60 Hz. Receptacles for these branchcircuits are equipped with CSA/NEMA 6-15R straight-blade receptacles.


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Gas Supply RequirementsRefer to Gas Supply Requirements on page 16.


Installation of gas supplies and connections complies with local regulations andsafety standards.

Gas 1/Gas 2 is available:• Zero-grade air or a SCIEX-recommended gas generator

• 5500 systems: Delivery pressure of 100 psi (6.89 bar), maximum, withflows up to 22 L/min

• 6500/6500+ systems: Delivery pressure of 100 psi (6.89 bar), maximum,with flows up to 26 L/min

Source exhaust gas is available:• Clean, dry, and oil-free air or ultra-high purity (UHP) nitrogen (99.999%) or

a SCIEX-recommended gas generator

• 5500 systems: Delivery pressure of 55 psi (3.79 bar) minimum with flowsup to 17 L/min

• 6500/6500+ systems: Delivery pressure of 55 psi (3.79 bar) minimum withflows up to 25 L/min

Curtain GasTM supply is available:• UHP nitrogen or a SCIEX-recommended gas generator

• 5500 systems: Delivery pressure of 60 psi (4.14 bar), maximum, with flowsup to 13 L/min

• 6500/6500+ systems: Delivery pressure of 60 psi (4.14 bar), maximum,with flows up to 18 L/min


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Ventilation and Waste-Collection RequirementsRefer to Ventilation and Waste Collection Requirements on page 19.

CompleteRequirement

Installation of plumbing and ventilation fixtures complies with local regulations and safetystandards.

Ventilation of the laboratory environment in which the mass spectrometer will be used complieswith local regulations and the air exchange rate is appropriate for the work performed.A minimum of 10 air exchanges/hour is required.

A negative flow vent is provided with a total flow rate capacity of 283 L/min (10 cfm), asmeasured at the inlet to the laboratory ventilation system.

A smooth fitting is provided for the roughing pump, with an outside diameter (o.d.) of 3.2 cm(1.25 inches). The vent is within 1.5 m (60 inches) of the exhaust port on the roughing pumpand at least 1 m (40 inches) above the floor.

A fitting is provided for the source exhaust drain bottle with an o.d. of 2.5 cm (1.0 inch). Thevent is within 1.5 m (60 inches) of the source exhaust drain bottle.

Environmental RequirementsRefer to Environmental Requirements on page 20.


The size of the laboratory is at least 31.7 cubic meters (1120 cubic feet).

An ambient temperature of 15 °C to 30 °C (59 °F to 86 °F) is maintained.Over time, the temperature remains within a range of with the rate of the changein temperature not exceeding 2 °C (3.6 °F) per hour. Ambient temperaturefluctuations exceeding the limits might result in mass shifts in spectra.

Relative humidity is 35% to 50%, non-condensing.

Air conditioning provides a minimum of• 5500 systems: 6500 Btu/hr

• 6500/6500+ systems: 8700 Btu/hr


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BioSafety Requirements

Refer to BioSafety Requirements on page 21.

CompleteRequirement

The site is not designated as BioSafety Level 3 (BSL-3) or BioSafety Level 4 (BSL-4).

Solutions and Equipment RequirementsRefer to Customer-Supplied Solutions and Equipment on page 22.

CompleteRequirement

All required solutions and bottles are available.

Product Familiarization


An account has been obtained on the SCIEX Learning Portal, and the onlinelearning modules have been completed.Go to sciex.com/LearningPortal and then click the link to request an account.When the account information is received, complete the online learning modules.

System documentation has been obtained and reviewed.


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#

Comments and Exceptions

Signoff

Completion date(yyyy-mm-dd)

Site planner contact name

I acknowledge that all of the installation requirements, as specified in this document, havebeen met.

Return date(yyyy-mm-dd)

FSE name

FSE e-mail


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Site Layout RequirementsReturn to checklist.

Weights and Dimensions

WeightLengthWidthHeightEquipment

130 kg (285 lbs)79 cm (32 inches)79 cm (32 inches)59 cm (24inches)

Mass spectrometer

13 kg (29 lbs)53 cm (21 inches)66 cm (26 inches)19 cm (8 inches)SelexION® controller

module


5500 systems:Instrument bench


6500/6500+ systems:Instrument bench withgas generator


6500/6500+ systems:Instrument bench withoutgas generator

Installation Site Clearances

Clearance RequiredSide

1 m (39 inches) for service accessFront, left, and right

0.7 m (28 inches) for service accessBack


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ASite Requirements

Electrical RequirementsReturn to checklist.

WARNING! Electrical Shock Hazard. Use only qualified personnel for the installationof all electrical supplies and fixtures, and make sure that all installations adhere tolocal regulations and safety standards.

WARNING! Electrical Shock Hazard. Do not connect the SelexION®or SelexION

®+

technology to the same branch circuit as either the mass spectrometer or theroughing pump.

The total combined system (mass spectrometer, roughing pump, and SelexION® or SelexION

®+ technology) powerconsumption is• 5500 systems: 3400 VA (50 Hz or 60 Hz) at 230 VAC

• 6500/6500+ systems: 4600 VA (50 Hz or 60 Hz) at 230 VAC

Mains Supply Connections• Provide a branch circuit for the SelexION

® or SelexION

®+ technology. The SelexION® controller module can be

connected to the same branch circuit as the acquisition computer.

• For installations outside North America, use locally approved standard connections and cables.

• For installation in North America, use a 15 A branch circuit equipped with NEMA 6-15R straight blade receptacles(208 VAC or 240 VAC).

An external line transformer is not required.

Mains Supply FluctuationsIn areas where the mains supply is subject to voltage fluctuations exceeding of the nominal value a powerconditioner is required. High or low voltages can adversely affect the electronic components of the massspectrometer. Refer to Uninterruptible Power Supply or Power Conditioner on page 15.

Protective Earth Conductor

WARNING! Electrical Shock Hazard. Do not intentionally interrupt the protectiveearth conductor. Any interruption of the protective earth conductor will create anelectrical shock hazard.

The mains supply must include a correctly installed protective earth conductor that must be installed or checkedby a qualified electrician before the system is connected.


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Site Requirements

Uninterruptible Power Supply or Power ConditionerIf the SelexION

® or SelexION

®+ technology is powered by a UPS or power conditioner, then it must meet thefollowing specifications.

Table A-1 UPS and Power Conditioner Requirements

RequirementSpecification

200 VAC to 240 VAC True on line (double conversion)Output voltage

50 Hz or 60 HzFrequency

Pure sine-waveWaveform

3 × nominal currentMinimum peak current

< 3%Output voltage distortion

Circuit breakerOutput protection

• 5500 systems: 4000 VA

• 6500/6500+ systems: 5000 VA

Minimum power requirement

System Electrical Specifications

ValueSpecification

200 VAC to 240 VACNominal input voltage

± 10% of nominalInput voltage fluctuation

50 Hz or 60 HzFrequency

5 AMaximum input current

1200 VAMaximum input power


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Site Requirements

Gas Supply RequirementsReturn to checklist.

WARNING! Radiation Hazard, Biohazard, or Toxic Chemical Hazard. Use onlyqualified personnel for the installation of all gas supplies and connections,and make sure that all installations adhere to local regulations and safetystandards.

CAUTION: Potential System Damage. Regulate each supply separately at the massspectrometer to prevent over pressure damage to the mass spectrometer.

CAUTION: Potential System Contamination. Use compression fittings for gas line connections.Do not use liquid pipe sealant. If threaded fittings must be used, then Teflon tape can beused to seal the threads. Do not use soldered fittings unless the tubing is thoroughlycleaned afterwards.

The 5500 or 6500 series system equipped with the SelexION® technology or the 6500+ series system equipped

with the SelexION®+ technology requires specific gas flows. Use high-pressure liquid nitrogen dewars or

SCIEX-recommended gas generators.

Figure A-1 Recommended Gas Connections


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Site Requirements

TubingFlow Rate(max)

PressureDescriptionGasItem

1/4 inch (0.64 cm)• 5500systems: 22L/min

• 6500/6500+

systems: 26L/min

100 psi (6.89 bar)maximum

Zero grade air onlyGas 1/Gas 21


• 6500/6500+

systems: 18L/min


UHP N2 onlyCurtain GasTM

supply; CAD gas2


• 6500/6500+

systems: 25L/min


Clean, dry, oil-freeair or UHP N2

Source exhaust3

Note: Gas pressures and flow rates must be measured at the inlet to the mass spectrometer.

Note: Gas quality, flows, and pressures must meet the requirements in Figure A-1, or a SCIEX-approved gasgenerator must be used.

Note: The air input fitting on the back panel is a quick connect, shown in Figure A-2. The nitrogen input fittingis a 1/4-inch Swagelok, shown in Figure A-3.


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Site Requirements

Figure A-2 Quick-connect Fitting

Figure A-3 Swagelok Connection and Tubing

Optional Gas GeneratorsGas generators are available from SCIEX. Refer to the documentation supplied by the manufacturer for specificationsand for the number and type of outlets required.


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Site Requirements

Ventilation and Waste Collection RequirementsReturn to checklist.

WARNING! Radiation Hazard, Biohazard, or Toxic Chemical Hazard. Makesure that the mass spectrometer is properly vented and that good generallaboratory ventilation is provided. Adequate laboratory ventilation is requiredto control solvent and sample emissions and to provide for safe operationof the mass spectrometer.

WARNING! Radiation Hazard, Biohazard, or Toxic Chemical Hazard. Use onlyqualified personnel for the installation of plumbing and ventilation fixtures,and make sure that all installations follow local bylaws and regulations.

CAUTION: Potential System Damage. Do not connect the source exhaust hose to the vent.The connection must be made by a qualified FSE.

• Provide a ventilation system with a total negative flow rate capacity of 283 L/min (10 cfm) at item 1 in FigureA-4 and a minimum of 10 air exchanges per hour.

• Provide a smooth fitting for the roughing pump, with an outside diameter (o.d.) of 3.2 cm (1.25 inches). Thevent must be within 1.5 m (60 inches) of the exhaust port on the roughing pump, and at least 1 m (40 inches)above the floor. Refer to Figure A-4.

• Provide a fitting for the source exhaust drain bottle with an o.d. of 2.5 cm (1.0 inch). The vent must be within1.5 m (60 inches) of the source exhaust drain bottle. Refer to Figure A-4.


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Site Requirements

Figure A-4 Vent Connections

DescriptionItem

Vent1

Source exhaust hose: 2.5 cm (1 inch) inside diameter (i.d.)2

Roughing pump exhaust hose: 3.2 cm (1.25 inches) i.d.3

Source exhaust drain bottle. Make sure that the bottle is secured at all times to prevent spills.4

Source exhaust drain tubing: 1.6 cm (0.625 inch) i.d.5

Note: Source exhaust hose connections at the drain bottle, mass spectrometer, and the lab vent are securedwith hose clamps.

Environmental RequirementsReturn to checklist.

• Minimum room size of 31.7 cubic meters (1120 cubic feet)


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Site Requirements

• An ambient temperature of 15°C to 30°C (59°F to 86°F)Over time, the temperature must remain within a range of 4 °C (7.2 °F), with the rate of the change intemperature not exceeding 2°C (3.6°F) per hour. Ambient temperature fluctuations exceeding the limits mightresult in mass shifts in spectra.

• Relative humidity from 35% to 50%, non-condensing

• Cooling and venting for the mass spectrometer and roughing pump: 16 000 Btu/hr

• 5500 systems: 6500 Btu/hr

• 6500/6500+ systems: 8700 Btu/hr

Note: Operation of the mass spectrometer at altitudes higher than 2000 m (6400 feet) above sea level mightimpact mass spectrometer operation.

BioSafety RequirementsThe site must not be designated BioSafety Level 3 (BSL-3) or BioSafety Level 4 (BSL-4). SCIEX does not install,service, or repair SCIEX systems in areas designated BSL-3 or BSL-4.

Sound Pressure Level

ValueSound Pressure

5500 Systems

65 dBAAverage sound pressure level

68 dBAMaximum sound pressure level (at the ion source)

6500 and 6500+ Systems

64 dBAAverage source pressure level

74 dBAMaximum sound pressure level (at the ion source)

A sound pressure level of 85 dBA above a reference sound pressure of 20 μPa is at present regarded by manyauthorities as the threshold at which a hazard might be caused. Special means, such as the use of protective earpieces, can make a higher sound pressure level non-hazardous to the operator.

SCIEX recommends that the sound pressure level be measured or calculated by the user both at the operator'sposition in normal use and at whatever point 1 m from the enclosure of the equipment has the highest soundpressure level.


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Site Requirements

Vibration• The packaged mass spectrometer was tested to confirm that the effects of transportation do not affect mass

spectrometer performance upon arrival at the customer destination.

• The mass spectrometer is designed to operate in any laboratory environment that accommodates precisionanalytical instrumentation.

Customer-Supplied Solutions and EquipmentReturn to checklist.

WARNING! Toxic Chemical Hazard. Refer to chemical product Safety Data Sheetsand follow all safety guidelines when handling, storing, and disposing of chemicals.

Note: Do not use polyethylene vials or bottles.

In addition to the standard solutions required for the mass spectrometer, the following solutions must be suppliedfor use with the SelexION

® or SelexION

®+ technology.

• MS-grade acetonitrile

• MS-grade methanol

• MS-grade acetone (2 L)

• MS-grade isopropanol (2 L)

Table A-2 Modifier Consumption at 20 psi Curtain GasTM Flow Rate (µL per minute)

ConcentrationModifier

10%3.0%1.5%

962.558267.928131.924Methanol

1250.425348.056171.378Acetonitrile

1821.856507.115249.696Isopropanol

1746.182486.051239.324Acetone


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Site Requirements

Table A-3 Modifier Consumption at 20 psi Curtain GasTM Flow Rate (mL per hour)


10%3.0%1.5%

57.75316.0767.915Methanol

75.02520.88310.283Acetonitrile

109.31130.42714.982Isopropanol

104.77129.16314.359Acetone

Table A-4 Modifier Consumption at 20 psi Curtain GasTM Flow Rate (mL per 24 hours)


10%3.0%1.5%

1386.083385.817189.971Methanol

1800.611501.201246.784Acetonitrile

2623.473730.245359.562Isopropanol

2514.502699.913344.627Acetone


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Site Requirements

CategoryDescription

Pollution Degree 2Equipment pollution degree

Overvoltage Category IIMains supply transient overvoltage

Note: Environments with a Pollution Degree 2 rating include laboratories and sales and commercial areas.

For more information, refer to the International Electrotechnical Commission standards IEC 61010-1 and IEC 60364.


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BEquipment Safety Categories

DateReason for ChangeRevision

June 2015First release of document. Based on D5024211 B, with new templateapplied, addition of 6500+ and SelexION

®+ technology information, andupdates to ventilation and environmental requirements.

A

September 2015Correct gas specification for Gas 1/Gas 2.B

December 2015Correct gas specification for Curtain GasTM flow and restore missing tubingfor Curtain GasTM flow.

C


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Revision History

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