Semen Preparation Semen Preparation using the Zavos Swim-using the Zavos Swim-

up Column (ZSC)up Column (ZSC)

Dr. Panayiotis M. Zavos, Ed.S., Ph.D.Dr. Panayiotis M. Zavos, Ed.S., Ph.D.President and CEOPresident and CEOZavos Diagnostic Laboratories, Inc.Zavos Diagnostic Laboratories, Inc.Lexington, KentuckyLexington, KentuckyUSAUSA

““We did all this for you”We did all this for you”

Click to continueClick to continue

Zavos Swim-up ColumnZavos Swim-up Column

The ZSC-II Kit The ZSC-II Kit includes ZSC-II, includes ZSC-II, plastic pipette, plastic pipette,

and 1mL syringe and 1mL syringe with needle.with needle.

Click to continueClick to continue

Zavos Swim-up ColumnZavos Swim-up Column

Additional Additional materials:materials: SpermPrep MediaSpermPrep Media LabTop incubatorLabTop incubator IUI CatheterIUI Catheter

Click to continueClick to continue

Zavos Swim-up ColumnZavos Swim-up Column

Remove ZSC Remove ZSC from the pack from the pack and using sterile and using sterile techniques, techniques, uncap the ZSC.uncap the ZSC.

Click to continueClick to continue

Zavos Swim-up ColumnZavos Swim-up Column

Add 2mL of semen Add 2mL of semen directly into the bottom directly into the bottom cone of the ZSC using cone of the ZSC using the plastic pipette.the plastic pipette.

Click to continueClick to continue

Zavos Swim-up ColumnZavos Swim-up Column

If the volume of semen If the volume of semen is less than 2mL and is less than 2mL and does not reach the top does not reach the top of the cone, then gently of the cone, then gently add SpermPrep Media add SpermPrep Media up to the top of the up to the top of the cone to make up the cone to make up the difference.difference.

Click to continueClick to continue

Zavos Swim-up ColumnZavos Swim-up Column

Using the syringe with Using the syringe with needle, add 0.7mL of needle, add 0.7mL of SpermPrep Media on the SpermPrep Media on the outside of the cone up to outside of the cone up to the top of the cone until the top of the cone until the media touches and the media touches and covers the semen.covers the semen.

““This allows the healthy sperm to swim This allows the healthy sperm to swim out of the semen and into the media.”out of the semen and into the media.”

Click to continueClick to continue

Zavos Swim-up ColumnZavos Swim-up Column

Place the ZSC Place the ZSC in the LabTop in the LabTop Incubator which Incubator which is set at 35°Cis set at 35°C

Click to continueClick to continue

Zavos Swim-up ColumnZavos Swim-up Column

Leave the ZSC Leave the ZSC in the LabTop in the LabTop Incubator for 45-Incubator for 45-60 minutes. 60 minutes.

““This allows adequate This allows adequate numbers of high quality numbers of high quality sperm to swim into the sperm to swim into the over layered media.”over layered media.”

Click to continueClick to continue

Zavos Swim-up ColumnZavos Swim-up Column

Aspirate all the media, Aspirate all the media, which should contain which should contain all the motile and all the motile and healthy sperm, from healthy sperm, from the outside of the cone.the outside of the cone.

Click to continueClick to continue

Zavos Swim-up ColumnZavos Swim-up Column

Simply, attach the Simply, attach the syringe to the catheter syringe to the catheter of your choice (TomCat of your choice (TomCat shown) and perform shown) and perform the Intra Uterine the Intra Uterine Insemination (IUI).Insemination (IUI).

““It is that simple!”It is that simple!”

Click to continueClick to continue

Zavos Swim-up ColumnZavos Swim-up ColumnTo order your ZSC Kits, LabTop To order your ZSC Kits, LabTop

incubator, SpermPrep Media, and IUI incubator, SpermPrep Media, and IUI Catheters, please call us at Catheters, please call us at

1-800-998-4567 or1-859-278-68061-800-998-4567 or1-859-278-6806

You may also e-mail us you order at You may also e-mail us you order at info@zdlinc.cominfo@zdlinc.com

or order online at or order online at www.zdlinc.comwww.zdlinc.com