Date post: | 07-Aug-2015 |
Category: |
Documents |
Upload: | nitin-kanwale |
View: | 14 times |
Download: | 2 times |
A Novel lipoprotein-mimic nanocarrier composed of the modified protein and lipid for tumor cell targeting delivery
Presented By:
Mr. Nitin P. Kanwale M. Pharm. (Quality Assurance)
MVP-SAMAJ’S COLLEGE OF PHARMACY, NASHIK02.
Content
Introduction
Synthesis of Modified protein
Preparation of Modified Lipid Nanocarrier
Characterisation
Cytotoxicity Study
Conclusion
References
Objective of Research
Develop the Nanocomplex that mimic the structure of lipoprotein
To achieve Tumour cell targeting
To overcome shortages of lipoprotein
Modified protein-lipid nanocomplex
The mP-LNC was composed of a shell of selectable targeting ligand modified protein with amphiphilic phospholipid and a core of hydrophobic lipids.
Synthesis and characterization of ursodeoxycholic acid modified BSA
The carboxyl group of ursodeoxycholic acid (UA) was reactivated by EDC and NHS and then covalently attached to the lysine residues of BSA.
• Degree of substitution of modified BSA • Molecular weight of modified BSA
• Surface tension measurement of modified BSA
Preparation of modified protein–lipid nanocomplex (mP-LNC)
LNP was composed of PC (phosphatidylcholine), MCT (medium chain triglycerides), octadecylamine (OL) and hydrophobic drug. nP-LNC was prepared by incubating natural BSA with LNP. mP-LNC included uP-LNC and cP-LNC, respectively prepared by incubating UA modified BSA (uP) and CH modified BSA (cP) with LNP.
Characterization of mP-LNC and LNP
Morphology, particle size and zeta potential
Determination of loading efficiency
WcWN *
100DLE%=
Panels a–c were the fluorescence microscopy images of L02 cells after incubation with (A) LNP, (B) c60P-LNC and (C) u60P-LNC (with 100 μg/mL of nanoparticle concentration)at 37 °C for 2 h. Panels d–f were the fluorescence microscopy images of Bel 7402 cells after incubation with (D) LNP, (E) c60P-LNC and (F) u60P-LNC (with 100 μg/mL of nanoparticleconcentration) at 37 °C for 2 h. Panels g–i were the fluorescence microscopy images of HepG2 cells after incubated with (G) LNP, (H) c60P-LNC and (I) u60P-LNC (with 100 μg/mL ofnanoparticle concentration) at 37 °C for 2 h.
Conclusion
A novel modified protein–lipid Nano complex was developed inthis study.The targeting effect of the protein–lipid Nano complex was preliminarily proved by using coumarin-6 as fluorescence probe in normal and cancer liver cells. It was observed that the uptake of uP-LNC in hepatoma cells significantly increased compared with that of cP-LNC or LNP, and was much more in cancer cells than in normal cells. It indicated that the recognition of UA and bile acid receptor played an important role in the targeting delivery.
Review
References
Ying Xu, Xuefeng Jin, Qineng Ping , Juan Cheng, Minjie Sun, A ⁎novel lipoprotein-mimic nanocarrier composed of the modified protein and lipid for tumor cell targeting delivery, Journal of Controlled Release 146 (2010) 299–308